Difference between revisions of "General Information/ IS derivatives of Tn3 family transposons"
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− | '''<big>A</big>'''nother source of ambiguity for classification purposes occurs in the Tn''3'' family (see section “[[Transposons families|Tn families]]” and "[[Transposons families/Tn3 family|Tn''3'' family]]") [[:Image:1.15.1.png|(Fig.10.1]] and [[:Image:1.15.1.png|10.2)]] <ref><nowiki><pubmed>26350313</pubmed></nowiki></ref>. Tn''3'' family members are quite variable. They include a number of diverse passenger genes that can represent entire operons, notably mercury resistance, or individual genes involved in antibiotic resistance, breakdown of halogenated aromatics, or virulence [e.g. <ref><nowiki><pubmed>10477306</pubmed></nowiki></ref> | + | '''<big>A</big>'''nother source of ambiguity for classification purposes occurs in the Tn''3'' family (see section “[[Transposons families|Tn families]]” and "[[Transposons families/Tn3 family|Tn''3'' family]]") [[:Image:1.15.1.png|(Fig.10.1]] and [[:Image:1.15.1.png|10.2)]] <ref><nowiki><pubmed>26350313</pubmed></nowiki></ref>. Tn''3'' family members are quite variable. They include a number of diverse passenger genes that can represent entire operons, notably mercury resistance, or individual genes involved in antibiotic resistance, breakdown of halogenated aromatics, or virulence [e.g. <ref><nowiki><pubmed>10477306</pubmed></nowiki></ref>]. They often carry integron recombination platforms, enabling them to incorporate additional resistance genes by recruiting integron cassettes <ref><nowiki><pubmed>16845431</pubmed></nowiki></ref>. Members are quite characteristic: they have long relatively well conserved IR and a particularly long Tpase (950 to 1025 aa). They also encode a site-specific recombination (“resolution”) system necessary for completion of their transposition ([https://www.asmscience.org/content/book/10.1128/9781555817954.chap14 Grindley 2002] - Chapter 14 from [https://www.asmscience.org/content/book/10.1128/9781555817954 Mobile DNA II] book). There are a number of different resolution systems associated with different members of this family [[:Image:1.15.2.png|(Fig.10.2)]] [https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS1071 IS''1071''], [[:Image:1.15.1.png|(Fig.10.1)]] composed of Tn''3''-like IR and Tpase gene but lacking both the site-specific recombination system and passenger genes was identified many years ago<ref><nowiki><pubmed>1656436</pubmed></nowiki></ref><ref><nowiki><pubmed>8117095</pubmed></nowiki></ref>. This clearly accords with the definition of an IS. Several other examples have now been identified (e.g. [https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISVsa19 IS''Vsa19''], [https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISShfr9 IS''Shfr9''], [https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISBusp1 IS''Busp1'']). |
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|[[Image:1.15.1.png|thumb|center|510x510px|'''Fig.10.1.''' '''Tn''3'' family characteristics.'''<br />Transposons are shown as yellow boxes with their names on the left and their lengths on the right. Terminal inverted repeats are shown as black arrowheads within the box. Transposition-related genes (transposases and resolvase recombinases are shown as purple arrows), passenger genes as red arrows, for antibiotic resistance orange/yellow arrows for heavy metal resistance, bright yellow for plant pathogenicity genes orange for toxin/antitoxin genes and blue for integron integrase genes. Resolution sites are shown in green. | |[[Image:1.15.1.png|thumb|center|510x510px|'''Fig.10.1.''' '''Tn''3'' family characteristics.'''<br />Transposons are shown as yellow boxes with their names on the left and their lengths on the right. Terminal inverted repeats are shown as black arrowheads within the box. Transposition-related genes (transposases and resolvase recombinases are shown as purple arrows), passenger genes as red arrows, for antibiotic resistance orange/yellow arrows for heavy metal resistance, bright yellow for plant pathogenicity genes orange for toxin/antitoxin genes and blue for integron integrase genes. Resolution sites are shown in green. | ||
− | Note that Tn''1071'' carries only a transposase gene and therefore could be considered an Insertion sequence.|alt=|border]] | + | Note that [https://tncentral.ncc.unesp.br/cgi-bin/tn_report.pl?id=Tn1071-M65135 Tn''1071''] carries only a transposase gene and therefore could be considered an Insertion sequence.|alt=|border]] |
|[[Image:1.15.2.png|thumb|center|510x510px|'''Fig.10.2.''' Tn''3'' family resolvase configuration. | |[[Image:1.15.2.png|thumb|center|510x510px|'''Fig.10.2.''' Tn''3'' family resolvase configuration. | ||
'''A)''' Tn''3'' has divergent transposase and resolvase (serine recombinase) genes with a res (resolution, recombination site between the divergent genes. It includes 3 sites at which resolvase binds (I, II and III). | '''A)''' Tn''3'' has divergent transposase and resolvase (serine recombinase) genes with a res (resolution, recombination site between the divergent genes. It includes 3 sites at which resolvase binds (I, II and III). | ||
− | '''B)''' Tn''501'' carries co-linear transposase and resolvase genes with the res site upstream of the resolvase. | + | '''B)''' [https://tncentral.ncc.unesp.br/cgi-bin/tn_report.pl?id=Tn501-Z00027 Tn''501''] carries co-linear transposase and resolvase genes with the res site upstream of the resolvase. |
− | '''C)''' Tn''4430'' has a similar configuration as Tn''501'' but its resolvase is a tyrosine recombinase and its res site, upstream of the resolvase has a more complex structure | + | '''C)''' [https://tncentral.ncc.unesp.br/cgi-bin/tn_report.pl?id=Tn4430-X07651.1 Tn''4430''] has a similar configuration as [https://tncentral.ncc.unesp.br/cgi-bin/tn_report.pl?id=Tn501-Z00027 Tn''501''] but its resolvase is a tyrosine recombinase and its res site, upstream of the resolvase has a more complex structure |
− | '''D)''' Tn''Xax1'' carries two divergent genes, ''tnpS'' (a serine recombinase) and ''tnpT'' (a helper gene). The resolution site lies between the two genes and has a complex structure (figure derived from <ref><nowiki><pubmed>32234815</pubmed></nowiki></ref>). |alt=]] | + | '''D)''' [https://tncentral.ncc.unesp.br/cgi-bin/tn_report.pl?id=TnXax1-AE008925 Tn''Xax1''] carries two divergent genes, ''tnpS'' (a serine recombinase) and ''tnpT'' (a helper gene). The resolution site lies between the two genes and has a complex structure (figure derived from <ref><nowiki><pubmed>32234815</pubmed></nowiki></ref>). |alt=]] |
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==Bibliography== | ==Bibliography== | ||
<references /> | <references /> |
Revision as of 14:28, 9 August 2021
Another source of ambiguity for classification purposes occurs in the Tn3 family (see section “Tn families” and "Tn3 family") (Fig.10.1 and 10.2) [1]. Tn3 family members are quite variable. They include a number of diverse passenger genes that can represent entire operons, notably mercury resistance, or individual genes involved in antibiotic resistance, breakdown of halogenated aromatics, or virulence [e.g. [2]]. They often carry integron recombination platforms, enabling them to incorporate additional resistance genes by recruiting integron cassettes [3]. Members are quite characteristic: they have long relatively well conserved IR and a particularly long Tpase (950 to 1025 aa). They also encode a site-specific recombination (“resolution”) system necessary for completion of their transposition (Grindley 2002 - Chapter 14 from Mobile DNA II book). There are a number of different resolution systems associated with different members of this family (Fig.10.2) IS1071, (Fig.10.1) composed of Tn3-like IR and Tpase gene but lacking both the site-specific recombination system and passenger genes was identified many years ago[4][5]. This clearly accords with the definition of an IS. Several other examples have now been identified (e.g. ISVsa19, ISShfr9, ISBusp1).