IS1071

  • Family Tn3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
M65135 Y Alcaligenes sp.
Ralstonia metallidurans CH34
Ralstonia eutropha
Alcaligenes sp. BRC60 plasmid pBRC60
Alcaligenes sp. BRC60
Burkholderia cepacia st 2A
Comamonas testosteroni
DNA section
IS Length : 3201 bp

Ends


IR Length : 110

IRL : GGGGTCTCCTCGTTTTCAGTGCAATAAGTGACGGTACGCAAAGCTAGCAC
IRR : GGGGTCTCCTCGTTTTCAGTGCAATAAGTGACGGTACGAAAAGCTAGCAC

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GGGGTCTCCTCGTTTTCAGTGCAATAAGTGACGGTACGCAAAGCTAGCACTGGCGCGGGGGTGGTCTGGGTAGACCGTTGATTTCATTGACTTTCCTGTT
CGCTTTGTAAACGGGTATGGTGGCCTCCCACTTTTGAGGTTCACGATGCAGGGTTGGCACACAACGTTTTTGGGGATGCGTGGGCTCCCCCGCGATATCA
GCGACTTCGAGATGAAGGCATTTTTCACCTTCGATGGTGCCGAGCGCGACGCAATCAATGCACGCCGAGGTGATTCCCACAAGCTTGGTCTGGCGCTCCA
TATTGGTTTCCTGCGCATGAGTGGGCGTTTGCTCGGTGCCTTTCGGGTAATTCCAGTAGCCTTGTGGCGCCACCTTGGCAACGAGCTTGGCATTGCAGCA
CCAGAAGTCGCCTCGCTGAGAGCCATGTATGAACGCGGGCGCACGCTATTCGATCACCAACAAGTAGCCTGCACGGTCCTTGGATTCCAGTGGATGAGCG
AGCACCAGCGCCGCTCACTGGTACGTGAACTGCGCGACGAAGTGGCCGGCTGCGACCGCGATCAGCTACTCGTGCGGGCGCGTCAATGGCTGTACAAGAA
CAAGCTGGTGATCGTGCACGAGCGGGCAATTCGGACACTGATTGCGGCGGCACTTGCCCAGCTTGAAGTTGAAACAGGCACCGCCATCGCCGCCAGCGTT
GATCCAGCAACACTTGATCGCTGGCGAGCCTCAGTTTCAGAGCTGCGCCCAGATGGACAAACCCAGCAGAGTTGGCTATGGGCTGCACCGGCGAAACACT
CAACCCGCCAAATCAGCGAGGTACTGGAGCGCATCGACCTGCTTTACACGCTGGACGTTCATAAGCACCTGGCAGACATCCCCGATCTCATCTTGCGCCG
CTACGCGCGCCGACTTGTCTCCAGGCCGCCCTCAGCCGGAGCCAAGATCAAAGAGCCAGCGCGCACCGTGGAGGTCGCATGCTTTCTTCGGTATTGCCTG
TTCACCACCACAGACCAGTTGATCCTTATGGTGCAGCGCCGGATCGCCGATCTGTGGCGTCAGGCTGCCGCCGATGTCCCCGCTACCGTCAATTGGGCCG
CAATGTACAAAACGCTGCTCGGCGAACTTGTTGCCTTGAGCGCGCAAGGTGCGGTGCCAGATGCTGAGTTGCGTGCCCGTCTTGAAGCCTTGATCACCGA
AACCCAGAAACGCAAACCACCGAGCAGGGCCTCCCTGGTCCGCGAGGGATTGATTGATGGAATTCGCCCCGTGCGGTCGTTGCTCGTCGCCATTGCAAAG
CTGCCCTGGCAGGCCACCGGCGAGCATCCTGCCATCGAGTACCTTGCCAAGCTGCAAGCTTTATATCTCAAAGGATCCAGAAAGCTGCCAGTTGAAGTGG
TGGCACCAAGTCTGGGAATGATCTGGCAGGTTTCGATCTCCAGCCCAGACCGGGAACGGGCGTTTCAGGCGTTGGAGGTGGCCACCCTGTTTGCCCTGCG
CCGCGCGGTGCGCAATGGCTCGGTCTGGATTGAGCACAGCCTGAGCTTTCGGGGTCGTGCGCGCTTGTTCTTCACGGACGAGCGTTGGCAGGCAGAGTCC
AAGAAACACTATGCCCGTCTATCGTTACCCAGCAAGGCTGCCACTTTCTTGAAGCCTTTGCTGGCCAGAGTAACTGCCGGTGTCGATGCGGTGGCCGCTG
CAGCCCGCAGTGGCGTACTGCGCGTGGATGATGAACTCCATTTGTCGCCATTGCCCGCAGAGGACGAAGACCCAGAAGTGACCAAGCTGCGCGCGGCTTT
GGATCACCGCATCGGTGAGGTTCAATTGCCGGAAGTGATTCTGGCCGTTGACGCCCAGGTGCGCTTTAGCTGGATCATGCTCGGACGTGAGCCGCGCTCT
ACCGACGAGCTGCTGATGGTCTATGCCGGCATCATGGCCCACGGCACCAGTCTGACTGCGGTCGAATGCGCGCGCATGATTCCGCAATTGTCTGCCACCA
GCATTCGCCAGGCCATGCGCTGGGCGCGGGACGAACGGCGTCTGAGCCAGGCCTGCCAGGCTGTGCTGGAATTCATGCAGCGACACCCGATTGCCGCCAC
CTGGGGGCGGTCCGATTTGGCATCTTCTGACATGATGAGCATGGAGACCACCAAACGGGTGTGGCAAGCCCGGCTTGATCCTCGGCGCAACACACCTTCC
ATTGGAATCTACTCCCATGTAAAAGACCGGTGGGGCATCTTCCATGCGCAGCCCTTTGTGCTCAATGAGCGCCAGGCGGGCGTGGCCATTGAAGGTGTCA
TCCGCCAAGAAAAGCTGGAGACCAGCCAGCTTGCTGTGGATACCCATGGCTACACCGACTTTGCCATGTCACATGCCCGTTTGCTTGGTTTTGATCTTTG
CCCGCGGTTGAAGGAACTCAAACAGCGCCACCTCTTTGTGCCACGCGGCACCAAAGTGCCCGCAGAAATCGCTGCGGTGTGCGAAGCCAATGTCGACGTC
GCTTTGATCGAAAAGCATTGGGATAGTCTGGTGCACCTGGCAGCCTCGGTCATGAGCGGACATGCCAGTGCGGTGGCAGCTCTTGCGCGGTTCGGTTCTG
CCGCCCAGGGCGATCCAATCTATGAGGCTGGCGTGCAATTGGGGCGGTTGCTGCGTACGGCGTTTTTGGCTGACTACTTTGTCAAGGACGCTTTCAGGAA
CGAGTTGCGCCGGGTGCTCAATCGGGGCGAGGCTGTTAACGCCCTCAAGCGCGCCATTTATACCGGCCGGATCAGCCCGGCGCAGGCCAAACGTGTCGAT
GAAATGCAGGCTGTGGCCGATGCGTTGAGCCTGATGGCCAACATCGTGATGGCGTGGAATACCTCACAGATGCAGGCGGTCCTGGATCGCTGGTCGAACC
GCCGCCAGGTCATTCCACCGGAACTGATCGGGAAGATTGCGCCCACCAGGCTGGAGAGCATCAACTTGCGGGGTGTGTTTCGCTTCCCGGTTGACCGCTA
TGCTGACCAAATCCTGCCTTCGCGGCCAAATGCATCGATAACTGGCACCAATGGATGAAACCGACCACGGTTTGACGCCACGAATCGCAGATTTGAAAGT
GAACAGGAAAGTCAATGAAATCAACGATCTACCAACACCACCTCCGCGCCAGTGCTAGCTTTTCGTACCGTCACTTATTGCACTGAAAACGAGGAGACCC
C
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
2913 bp970 aa1463058+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MQGWHTTFLGMRGLPRDISDFEMKAFFTFDGAERDAINARRGDSHKLGLALHIGFLRMSGRLLGAFRVIPVALWRHLGNELGIAAPEVASLRAMYERGRT
LFDHQQVACTVLGFQWMSEHQRRSLVRELRDEVAGCDRDQLLVRARQWLYKNKLVIVHERAIRTLIAAALAQLEVETGTAIAASVDPATLDRWRASVSEL
RPDGQTQQSWLWAAPAKHSTRQISEVLERIDLLYTLDVHKHLADIPDLILRRYARRLVSRPPSAGAKIKEPARTVEVACFLRYCLFTTTDQLILMVQRRI
ADLWRQAAADVPATVNWAAMYKTLLGELVALSAQGAVPDAELRARLEALITETQKRKPPSRASLVREGLIDGIRPVRSLLVAIAKLPWQATGEHPAIEYL
AKLQALYLKGSRKLPVEVVAPSLGMIWQVSISSPDRERAFQALEVATLFALRRAVRNGSVWIEHSLSFRGRARLFFTDERWQAESKKHYARLSLPSKAAT
FLKPLLARVTAGVDAVAAAARSGVLRVDDELHLSPLPAEDEDPEVTKLRAALDHRIGEVQLPEVILAVDAQVRFSWIMLGREPRSTDELLMVYAGIMAHG
TSLTAVECARMIPQLSATSIRQAMRWARDERRLSQACQAVLEFMQRHPIAATWGRSDLASSDMMSMETTKRVWQARLDPRRNTPSIGIYSHVKDRWGIFH
AQPFVLNERQAGVAIEGVIRQEKLETSQLAVDTHGYTDFAMSHARLLGFDLCPRLKELKQRHLFVPRGTKVPAEIAAVCEANVDVALIEKHWDSLVHLAA
SVMSGHASAVAALARFGSAAQGDPIYEAGVQLGRLLRTAFLADYFVKDAFRNELRRVLNRGEAVNALKRAIYTGRISPAQAKRVDEMQAVADALSLMANI
VMAWNTSQMQAVLDRWSNRRQVIPPELIGKIAPTRLESINLRGVFRFPVDRYADQILPSRPNASITGTNG

 

Blast result :
Comments
IS1071 is a Tn3 family member lacking the resolvase gene. It is also found as part of plasmid pCPE3. Tn5271 is a 17kb composite transposon coding for chlorobenzoate degradation. It is delineoted by IS1071R and IS1071L.
99% DNA similar to IS1071 of Burkholderia cepacia st 2A (accession number AF029344 and AF029345 ) and 100% identical to IS1071 of Comamonas testosteroni (Accession number M65135 ).
Additional Accession number : AF225973 and AF225974 , Ralstonia eutropha, 100% DNA similar to the sequence of the database.
In Ralstonia metallidurans CH34, there is an insertion of 3 bp between position 554 and 555, this generate an insertion of one amino acid.
References
1] D. di Gioia, M. Peel, F. Fava and R. Campbell Wyndham (1998). Appl. Environ. Microbiol. 64, 1940-1946
2] Poh, R.P.C, Bruce, I.J. and Smith, A.R.W. (2001) Direct submission GenBank
3] Nakatsu,C., Ng,J., Singh,R., Straus,N. and Wyndham,C. (1991) PNAS. 88 (19), 8312-8316
4] Clement,P., Pieper,D.H. and Gonzalez,B. (2001) Microbiology 147 (Pt 8), 2141-2148