IS1071
- Family Tn3
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
M65135 | Y | Alcaligenes sp. | Ralstonia metallidurans CH34 Ralstonia eutropha Alcaligenes sp. BRC60 plasmid pBRC60 Alcaligenes sp. BRC60 Burkholderia cepacia st 2A Comamonas testosteroni |
DNA section
IS Length : 3201 bp
Ends
IR Length : 110
IRL : GGGGTCTCCTCGTTTTCAGTGCAATAAGTGACGGTACGCAAAGCTAGCAC
IRR : GGGGTCTCCTCGTTTTCAGTGCAATAAGTGACGGTACGAAAAGCTAGCAC
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|
DNA sequence
GGGGTCTCCTCGTTTTCAGTGCAATAAGTGACGGTACGCAAAGCTAGCACTGGCGCGGGGGTGGTCTGGGTAGACCGTTGATTTCATTGACTTTCCTGTT
CGCTTTGTAAACGGGTATGGTGGCCTCCCACTTTTGAGGTTCACGATGCAGGGTTGGCACACAACGTTTTTGGGGATGCGTGGGCTCCCCCGCGATATCA
GCGACTTCGAGATGAAGGCATTTTTCACCTTCGATGGTGCCGAGCGCGACGCAATCAATGCACGCCGAGGTGATTCCCACAAGCTTGGTCTGGCGCTCCA
TATTGGTTTCCTGCGCATGAGTGGGCGTTTGCTCGGTGCCTTTCGGGTAATTCCAGTAGCCTTGTGGCGCCACCTTGGCAACGAGCTTGGCATTGCAGCA
CCAGAAGTCGCCTCGCTGAGAGCCATGTATGAACGCGGGCGCACGCTATTCGATCACCAACAAGTAGCCTGCACGGTCCTTGGATTCCAGTGGATGAGCG
AGCACCAGCGCCGCTCACTGGTACGTGAACTGCGCGACGAAGTGGCCGGCTGCGACCGCGATCAGCTACTCGTGCGGGCGCGTCAATGGCTGTACAAGAA
CAAGCTGGTGATCGTGCACGAGCGGGCAATTCGGACACTGATTGCGGCGGCACTTGCCCAGCTTGAAGTTGAAACAGGCACCGCCATCGCCGCCAGCGTT
GATCCAGCAACACTTGATCGCTGGCGAGCCTCAGTTTCAGAGCTGCGCCCAGATGGACAAACCCAGCAGAGTTGGCTATGGGCTGCACCGGCGAAACACT
CAACCCGCCAAATCAGCGAGGTACTGGAGCGCATCGACCTGCTTTACACGCTGGACGTTCATAAGCACCTGGCAGACATCCCCGATCTCATCTTGCGCCG
CTACGCGCGCCGACTTGTCTCCAGGCCGCCCTCAGCCGGAGCCAAGATCAAAGAGCCAGCGCGCACCGTGGAGGTCGCATGCTTTCTTCGGTATTGCCTG
TTCACCACCACAGACCAGTTGATCCTTATGGTGCAGCGCCGGATCGCCGATCTGTGGCGTCAGGCTGCCGCCGATGTCCCCGCTACCGTCAATTGGGCCG
CAATGTACAAAACGCTGCTCGGCGAACTTGTTGCCTTGAGCGCGCAAGGTGCGGTGCCAGATGCTGAGTTGCGTGCCCGTCTTGAAGCCTTGATCACCGA
AACCCAGAAACGCAAACCACCGAGCAGGGCCTCCCTGGTCCGCGAGGGATTGATTGATGGAATTCGCCCCGTGCGGTCGTTGCTCGTCGCCATTGCAAAG
CTGCCCTGGCAGGCCACCGGCGAGCATCCTGCCATCGAGTACCTTGCCAAGCTGCAAGCTTTATATCTCAAAGGATCCAGAAAGCTGCCAGTTGAAGTGG
TGGCACCAAGTCTGGGAATGATCTGGCAGGTTTCGATCTCCAGCCCAGACCGGGAACGGGCGTTTCAGGCGTTGGAGGTGGCCACCCTGTTTGCCCTGCG
CCGCGCGGTGCGCAATGGCTCGGTCTGGATTGAGCACAGCCTGAGCTTTCGGGGTCGTGCGCGCTTGTTCTTCACGGACGAGCGTTGGCAGGCAGAGTCC
AAGAAACACTATGCCCGTCTATCGTTACCCAGCAAGGCTGCCACTTTCTTGAAGCCTTTGCTGGCCAGAGTAACTGCCGGTGTCGATGCGGTGGCCGCTG
CAGCCCGCAGTGGCGTACTGCGCGTGGATGATGAACTCCATTTGTCGCCATTGCCCGCAGAGGACGAAGACCCAGAAGTGACCAAGCTGCGCGCGGCTTT
GGATCACCGCATCGGTGAGGTTCAATTGCCGGAAGTGATTCTGGCCGTTGACGCCCAGGTGCGCTTTAGCTGGATCATGCTCGGACGTGAGCCGCGCTCT
ACCGACGAGCTGCTGATGGTCTATGCCGGCATCATGGCCCACGGCACCAGTCTGACTGCGGTCGAATGCGCGCGCATGATTCCGCAATTGTCTGCCACCA
GCATTCGCCAGGCCATGCGCTGGGCGCGGGACGAACGGCGTCTGAGCCAGGCCTGCCAGGCTGTGCTGGAATTCATGCAGCGACACCCGATTGCCGCCAC
CTGGGGGCGGTCCGATTTGGCATCTTCTGACATGATGAGCATGGAGACCACCAAACGGGTGTGGCAAGCCCGGCTTGATCCTCGGCGCAACACACCTTCC
ATTGGAATCTACTCCCATGTAAAAGACCGGTGGGGCATCTTCCATGCGCAGCCCTTTGTGCTCAATGAGCGCCAGGCGGGCGTGGCCATTGAAGGTGTCA
TCCGCCAAGAAAAGCTGGAGACCAGCCAGCTTGCTGTGGATACCCATGGCTACACCGACTTTGCCATGTCACATGCCCGTTTGCTTGGTTTTGATCTTTG
CCCGCGGTTGAAGGAACTCAAACAGCGCCACCTCTTTGTGCCACGCGGCACCAAAGTGCCCGCAGAAATCGCTGCGGTGTGCGAAGCCAATGTCGACGTC
GCTTTGATCGAAAAGCATTGGGATAGTCTGGTGCACCTGGCAGCCTCGGTCATGAGCGGACATGCCAGTGCGGTGGCAGCTCTTGCGCGGTTCGGTTCTG
CCGCCCAGGGCGATCCAATCTATGAGGCTGGCGTGCAATTGGGGCGGTTGCTGCGTACGGCGTTTTTGGCTGACTACTTTGTCAAGGACGCTTTCAGGAA
CGAGTTGCGCCGGGTGCTCAATCGGGGCGAGGCTGTTAACGCCCTCAAGCGCGCCATTTATACCGGCCGGATCAGCCCGGCGCAGGCCAAACGTGTCGAT
GAAATGCAGGCTGTGGCCGATGCGTTGAGCCTGATGGCCAACATCGTGATGGCGTGGAATACCTCACAGATGCAGGCGGTCCTGGATCGCTGGTCGAACC
GCCGCCAGGTCATTCCACCGGAACTGATCGGGAAGATTGCGCCCACCAGGCTGGAGAGCATCAACTTGCGGGGTGTGTTTCGCTTCCCGGTTGACCGCTA
TGCTGACCAAATCCTGCCTTCGCGGCCAAATGCATCGATAACTGGCACCAATGGATGAAACCGACCACGGTTTGACGCCACGAATCGCAGATTTGAAAGT
GAACAGGAAAGTCAATGAAATCAACGATCTACCAACACCACCTCCGCGCCAGTGCTAGCTTTTCGTACCGTCACTTATTGCACTGAAAACGAGGAGACCC
C
CGCTTTGTAAACGGGTATGGTGGCCTCCCACTTTTGAGGTTCACGATGCAGGGTTGGCACACAACGTTTTTGGGGATGCGTGGGCTCCCCCGCGATATCA
GCGACTTCGAGATGAAGGCATTTTTCACCTTCGATGGTGCCGAGCGCGACGCAATCAATGCACGCCGAGGTGATTCCCACAAGCTTGGTCTGGCGCTCCA
TATTGGTTTCCTGCGCATGAGTGGGCGTTTGCTCGGTGCCTTTCGGGTAATTCCAGTAGCCTTGTGGCGCCACCTTGGCAACGAGCTTGGCATTGCAGCA
CCAGAAGTCGCCTCGCTGAGAGCCATGTATGAACGCGGGCGCACGCTATTCGATCACCAACAAGTAGCCTGCACGGTCCTTGGATTCCAGTGGATGAGCG
AGCACCAGCGCCGCTCACTGGTACGTGAACTGCGCGACGAAGTGGCCGGCTGCGACCGCGATCAGCTACTCGTGCGGGCGCGTCAATGGCTGTACAAGAA
CAAGCTGGTGATCGTGCACGAGCGGGCAATTCGGACACTGATTGCGGCGGCACTTGCCCAGCTTGAAGTTGAAACAGGCACCGCCATCGCCGCCAGCGTT
GATCCAGCAACACTTGATCGCTGGCGAGCCTCAGTTTCAGAGCTGCGCCCAGATGGACAAACCCAGCAGAGTTGGCTATGGGCTGCACCGGCGAAACACT
CAACCCGCCAAATCAGCGAGGTACTGGAGCGCATCGACCTGCTTTACACGCTGGACGTTCATAAGCACCTGGCAGACATCCCCGATCTCATCTTGCGCCG
CTACGCGCGCCGACTTGTCTCCAGGCCGCCCTCAGCCGGAGCCAAGATCAAAGAGCCAGCGCGCACCGTGGAGGTCGCATGCTTTCTTCGGTATTGCCTG
TTCACCACCACAGACCAGTTGATCCTTATGGTGCAGCGCCGGATCGCCGATCTGTGGCGTCAGGCTGCCGCCGATGTCCCCGCTACCGTCAATTGGGCCG
CAATGTACAAAACGCTGCTCGGCGAACTTGTTGCCTTGAGCGCGCAAGGTGCGGTGCCAGATGCTGAGTTGCGTGCCCGTCTTGAAGCCTTGATCACCGA
AACCCAGAAACGCAAACCACCGAGCAGGGCCTCCCTGGTCCGCGAGGGATTGATTGATGGAATTCGCCCCGTGCGGTCGTTGCTCGTCGCCATTGCAAAG
CTGCCCTGGCAGGCCACCGGCGAGCATCCTGCCATCGAGTACCTTGCCAAGCTGCAAGCTTTATATCTCAAAGGATCCAGAAAGCTGCCAGTTGAAGTGG
TGGCACCAAGTCTGGGAATGATCTGGCAGGTTTCGATCTCCAGCCCAGACCGGGAACGGGCGTTTCAGGCGTTGGAGGTGGCCACCCTGTTTGCCCTGCG
CCGCGCGGTGCGCAATGGCTCGGTCTGGATTGAGCACAGCCTGAGCTTTCGGGGTCGTGCGCGCTTGTTCTTCACGGACGAGCGTTGGCAGGCAGAGTCC
AAGAAACACTATGCCCGTCTATCGTTACCCAGCAAGGCTGCCACTTTCTTGAAGCCTTTGCTGGCCAGAGTAACTGCCGGTGTCGATGCGGTGGCCGCTG
CAGCCCGCAGTGGCGTACTGCGCGTGGATGATGAACTCCATTTGTCGCCATTGCCCGCAGAGGACGAAGACCCAGAAGTGACCAAGCTGCGCGCGGCTTT
GGATCACCGCATCGGTGAGGTTCAATTGCCGGAAGTGATTCTGGCCGTTGACGCCCAGGTGCGCTTTAGCTGGATCATGCTCGGACGTGAGCCGCGCTCT
ACCGACGAGCTGCTGATGGTCTATGCCGGCATCATGGCCCACGGCACCAGTCTGACTGCGGTCGAATGCGCGCGCATGATTCCGCAATTGTCTGCCACCA
GCATTCGCCAGGCCATGCGCTGGGCGCGGGACGAACGGCGTCTGAGCCAGGCCTGCCAGGCTGTGCTGGAATTCATGCAGCGACACCCGATTGCCGCCAC
CTGGGGGCGGTCCGATTTGGCATCTTCTGACATGATGAGCATGGAGACCACCAAACGGGTGTGGCAAGCCCGGCTTGATCCTCGGCGCAACACACCTTCC
ATTGGAATCTACTCCCATGTAAAAGACCGGTGGGGCATCTTCCATGCGCAGCCCTTTGTGCTCAATGAGCGCCAGGCGGGCGTGGCCATTGAAGGTGTCA
TCCGCCAAGAAAAGCTGGAGACCAGCCAGCTTGCTGTGGATACCCATGGCTACACCGACTTTGCCATGTCACATGCCCGTTTGCTTGGTTTTGATCTTTG
CCCGCGGTTGAAGGAACTCAAACAGCGCCACCTCTTTGTGCCACGCGGCACCAAAGTGCCCGCAGAAATCGCTGCGGTGTGCGAAGCCAATGTCGACGTC
GCTTTGATCGAAAAGCATTGGGATAGTCTGGTGCACCTGGCAGCCTCGGTCATGAGCGGACATGCCAGTGCGGTGGCAGCTCTTGCGCGGTTCGGTTCTG
CCGCCCAGGGCGATCCAATCTATGAGGCTGGCGTGCAATTGGGGCGGTTGCTGCGTACGGCGTTTTTGGCTGACTACTTTGTCAAGGACGCTTTCAGGAA
CGAGTTGCGCCGGGTGCTCAATCGGGGCGAGGCTGTTAACGCCCTCAAGCGCGCCATTTATACCGGCCGGATCAGCCCGGCGCAGGCCAAACGTGTCGAT
GAAATGCAGGCTGTGGCCGATGCGTTGAGCCTGATGGCCAACATCGTGATGGCGTGGAATACCTCACAGATGCAGGCGGTCCTGGATCGCTGGTCGAACC
GCCGCCAGGTCATTCCACCGGAACTGATCGGGAAGATTGCGCCCACCAGGCTGGAGAGCATCAACTTGCGGGGTGTGTTTCGCTTCCCGGTTGACCGCTA
TGCTGACCAAATCCTGCCTTCGCGGCCAAATGCATCGATAACTGGCACCAATGGATGAAACCGACCACGGTTTGACGCCACGAATCGCAGATTTGAAAGT
GAACAGGAAAGTCAATGAAATCAACGATCTACCAACACCACCTCCGCGCCAGTGCTAGCTTTTCGTACCGTCACTTATTGCACTGAAAACGAGGAGACCC
C
Protein section
ORF number : 1
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
2913 bp | 970 aa | 146 | 3058 | + | No |
Chemistry : DDE
ORF sequence :
MQGWHTTFLGMRGLPRDISDFEMKAFFTFDGAERDAINARRGDSHKLGLALHIGFLRMSGRLLGAFRVIPVALWRHLGNELGIAAPEVASLRAMYERGRT
LFDHQQVACTVLGFQWMSEHQRRSLVRELRDEVAGCDRDQLLVRARQWLYKNKLVIVHERAIRTLIAAALAQLEVETGTAIAASVDPATLDRWRASVSEL
RPDGQTQQSWLWAAPAKHSTRQISEVLERIDLLYTLDVHKHLADIPDLILRRYARRLVSRPPSAGAKIKEPARTVEVACFLRYCLFTTTDQLILMVQRRI
ADLWRQAAADVPATVNWAAMYKTLLGELVALSAQGAVPDAELRARLEALITETQKRKPPSRASLVREGLIDGIRPVRSLLVAIAKLPWQATGEHPAIEYL
AKLQALYLKGSRKLPVEVVAPSLGMIWQVSISSPDRERAFQALEVATLFALRRAVRNGSVWIEHSLSFRGRARLFFTDERWQAESKKHYARLSLPSKAAT
FLKPLLARVTAGVDAVAAAARSGVLRVDDELHLSPLPAEDEDPEVTKLRAALDHRIGEVQLPEVILAVDAQVRFSWIMLGREPRSTDELLMVYAGIMAHG
TSLTAVECARMIPQLSATSIRQAMRWARDERRLSQACQAVLEFMQRHPIAATWGRSDLASSDMMSMETTKRVWQARLDPRRNTPSIGIYSHVKDRWGIFH
AQPFVLNERQAGVAIEGVIRQEKLETSQLAVDTHGYTDFAMSHARLLGFDLCPRLKELKQRHLFVPRGTKVPAEIAAVCEANVDVALIEKHWDSLVHLAA
SVMSGHASAVAALARFGSAAQGDPIYEAGVQLGRLLRTAFLADYFVKDAFRNELRRVLNRGEAVNALKRAIYTGRISPAQAKRVDEMQAVADALSLMANI
VMAWNTSQMQAVLDRWSNRRQVIPPELIGKIAPTRLESINLRGVFRFPVDRYADQILPSRPNASITGTNG
LFDHQQVACTVLGFQWMSEHQRRSLVRELRDEVAGCDRDQLLVRARQWLYKNKLVIVHERAIRTLIAAALAQLEVETGTAIAASVDPATLDRWRASVSEL
RPDGQTQQSWLWAAPAKHSTRQISEVLERIDLLYTLDVHKHLADIPDLILRRYARRLVSRPPSAGAKIKEPARTVEVACFLRYCLFTTTDQLILMVQRRI
ADLWRQAAADVPATVNWAAMYKTLLGELVALSAQGAVPDAELRARLEALITETQKRKPPSRASLVREGLIDGIRPVRSLLVAIAKLPWQATGEHPAIEYL
AKLQALYLKGSRKLPVEVVAPSLGMIWQVSISSPDRERAFQALEVATLFALRRAVRNGSVWIEHSLSFRGRARLFFTDERWQAESKKHYARLSLPSKAAT
FLKPLLARVTAGVDAVAAAARSGVLRVDDELHLSPLPAEDEDPEVTKLRAALDHRIGEVQLPEVILAVDAQVRFSWIMLGREPRSTDELLMVYAGIMAHG
TSLTAVECARMIPQLSATSIRQAMRWARDERRLSQACQAVLEFMQRHPIAATWGRSDLASSDMMSMETTKRVWQARLDPRRNTPSIGIYSHVKDRWGIFH
AQPFVLNERQAGVAIEGVIRQEKLETSQLAVDTHGYTDFAMSHARLLGFDLCPRLKELKQRHLFVPRGTKVPAEIAAVCEANVDVALIEKHWDSLVHLAA
SVMSGHASAVAALARFGSAAQGDPIYEAGVQLGRLLRTAFLADYFVKDAFRNELRRVLNRGEAVNALKRAIYTGRISPAQAKRVDEMQAVADALSLMANI
VMAWNTSQMQAVLDRWSNRRQVIPPELIGKIAPTRLESINLRGVFRFPVDRYADQILPSRPNASITGTNG
Blast result :
Comments
IS1071 is a Tn3 family member lacking the resolvase gene. It is also found as part of plasmid pCPE3. Tn5271 is a 17kb composite transposon coding for chlorobenzoate degradation. It is delineoted by IS1071R and IS1071L.
99% DNA similar to IS1071 of Burkholderia cepacia st 2A (accession number AF029344 and AF029345 ) and 100% identical to IS1071 of Comamonas testosteroni (Accession number M65135 ).
Additional Accession number : AF225973 and AF225974 , Ralstonia eutropha, 100% DNA similar to the sequence of the database.
In Ralstonia metallidurans CH34, there is an insertion of 3 bp between position 554 and 555, this generate an insertion of one amino acid.
99% DNA similar to IS1071 of Burkholderia cepacia st 2A (accession number AF029344 and AF029345 ) and 100% identical to IS1071 of Comamonas testosteroni (Accession number M65135 ).
Additional Accession number : AF225973 and AF225974 , Ralstonia eutropha, 100% DNA similar to the sequence of the database.
In Ralstonia metallidurans CH34, there is an insertion of 3 bp between position 554 and 555, this generate an insertion of one amino acid.
References
1] D. di Gioia, M. Peel, F. Fava and R. Campbell Wyndham (1998). Appl. Environ. Microbiol. 64, 1940-1946
2] Poh, R.P.C, Bruce, I.J. and Smith, A.R.W. (2001) Direct submission GenBank
3] Nakatsu,C., Ng,J., Singh,R., Straus,N. and Wyndham,C. (1991) PNAS. 88 (19), 8312-8316
4] Clement,P., Pieper,D.H. and Gonzalez,B. (2001) Microbiology 147 (Pt 8), 2141-2148
2] Poh, R.P.C, Bruce, I.J. and Smith, A.R.W. (2001) Direct submission GenBank
3] Nakatsu,C., Ng,J., Singh,R., Straus,N. and Wyndham,C. (1991) PNAS. 88 (19), 8312-8316
4] Clement,P., Pieper,D.H. and Gonzalez,B. (2001) Microbiology 147 (Pt 8), 2141-2148