Difference between revisions of "General Information/ IS derivatives of Tn3 family transposons"
Line 1: | Line 1: | ||
− | '''<big>A</big>'''nother source of ambiguity for classification purposes occurs in the Tn''3'' family (see section “[[Transposons families|Tn families]]” and "[[Transposons families/Tn3 family|Tn''3'' family]]") [[:Image:1.15.1.png|(Fig.10.1]] and [[:Image:1.15.1.png|10.2)]] <ref | + | '''<big>A</big>'''nother source of ambiguity for classification purposes occurs in the Tn''3'' family (see section “[[Transposons families|Tn families]]” and "[[Transposons families/Tn3 family|Tn''3'' family]]") [[:Image:1.15.1.png|(Fig.10.1]] and [[:Image:1.15.1.png|10.2)]] <ref><pubmed>26350313</pubmed></ref>. Tn''3'' family members are quite variable. They include a number of diverse passenger genes that can represent entire operons, notably mercury resistance, or individual genes involved in antibiotic resistance, breakdown of halogenated aromatics, or virulence [e.g. <ref><pubmed>10477306</pubmed></ref>]. They often carry integron recombination platforms, enabling them to incorporate additional resistance genes by recruiting integron cassettes <ref><pubmed>16845431</pubmed></ref>. Members are quite characteristic: they have long relatively well conserved IR and a particularly long Tpase (950 to 1025 aa). They also encode a site-specific recombination (“resolution”) system necessary for completion of their transposition ([https://www.asmscience.org/content/book/10.1128/9781555817954.chap14 Grindley 2002] - Chapter 14 from [https://www.asmscience.org/content/book/10.1128/9781555817954 Mobile DNA II] book). There are a number of different resolution systems associated with different members of this family [[:Image:1.15.2.png|(Fig.10.2)]] [https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS1071 IS''1071''], [[:Image:1.15.1.png|(Fig.10.1)]] composed of Tn''3''-like IR and Tpase gene but lacking both the site-specific recombination system and passenger genes was identified many years ago<ref><pubmed>1656436</pubmed></ref><ref><pubmed>8117095</pubmed></ref>. This clearly accords with the definition of an IS. Several other examples have now been identified (e.g. [https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISVsa19 IS''Vsa19''], [https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISShfr9 IS''Shfr9''], [https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISBusp1 IS''Busp1'']). |
<center> | <center> | ||
{| | {| | ||
Line 13: | Line 13: | ||
'''C)''' [https://tncentral.ncc.unesp.br/cgi-bin/tn_report.pl?id=Tn4430-X07651.1 Tn''4430''] has a similar configuration as [https://tncentral.ncc.unesp.br/cgi-bin/tn_report.pl?id=Tn501-Z00027 Tn''501''] but its resolvase is a tyrosine recombinase and its res site, upstream of the resolvase has a more complex structure | '''C)''' [https://tncentral.ncc.unesp.br/cgi-bin/tn_report.pl?id=Tn4430-X07651.1 Tn''4430''] has a similar configuration as [https://tncentral.ncc.unesp.br/cgi-bin/tn_report.pl?id=Tn501-Z00027 Tn''501''] but its resolvase is a tyrosine recombinase and its res site, upstream of the resolvase has a more complex structure | ||
− | '''D)''' [https://tncentral.ncc.unesp.br/cgi-bin/tn_report.pl?id=TnXax1-AE008925 Tn''Xax1''] carries two divergent genes, ''tnpS'' (a serine recombinase) and ''tnpT'' (a helper gene). The resolution site lies between the two genes and has a complex structure (figure derived from <ref | + | '''D)''' [https://tncentral.ncc.unesp.br/cgi-bin/tn_report.pl?id=TnXax1-AE008925 Tn''Xax1''] carries two divergent genes, ''tnpS'' (a serine recombinase) and ''tnpT'' (a helper gene). The resolution site lies between the two genes and has a complex structure (figure derived from <ref><pubmed>32234815</pubmed></ref>). |alt=]] |
|} | |} | ||
</center> | </center> | ||
==Bibliography== | ==Bibliography== | ||
<references /> | <references /> |
Revision as of 18:30, 9 August 2021
Another source of ambiguity for classification purposes occurs in the Tn3 family (see section “Tn families” and "Tn3 family") (Fig.10.1 and 10.2) [1]. Tn3 family members are quite variable. They include a number of diverse passenger genes that can represent entire operons, notably mercury resistance, or individual genes involved in antibiotic resistance, breakdown of halogenated aromatics, or virulence [e.g. [2]]. They often carry integron recombination platforms, enabling them to incorporate additional resistance genes by recruiting integron cassettes [3]. Members are quite characteristic: they have long relatively well conserved IR and a particularly long Tpase (950 to 1025 aa). They also encode a site-specific recombination (“resolution”) system necessary for completion of their transposition (Grindley 2002 - Chapter 14 from Mobile DNA II book). There are a number of different resolution systems associated with different members of this family (Fig.10.2) IS1071, (Fig.10.1) composed of Tn3-like IR and Tpase gene but lacking both the site-specific recombination system and passenger genes was identified many years ago[4][5]. This clearly accords with the definition of an IS. Several other examples have now been identified (e.g. ISVsa19, ISShfr9, ISBusp1).
Bibliography
- ↑ Nicolas E, Lambin M, Dandoy D, Galloy C, Nguyen N, Oger CA, Hallet B . The Tn3-family of Replicative Transposons. - Microbiol Spectr: 2015 Aug, 3(4); [PubMed:26350313] [DOI]
- ↑ Liebert CA, Hall RM, Summers AO . Transposon Tn21, flagship of the floating genome. - Microbiol Mol Biol Rev: 1999 Sep, 63(3);507-22 [PubMed:10477306] [DOI]
- ↑ Mazel D . Integrons: agents of bacterial evolution. - Nat Rev Microbiol: 2006 Aug, 4(8);608-20 [PubMed:16845431] [DOI]
- ↑ Nakatsu C, Ng J, Singh R, Straus N, Wyndham C . Chlorobenzoate catabolic transposon Tn5271 is a composite class I element with flanking class II insertion sequences. - Proc Natl Acad Sci U S A: 1991 Oct 1, 88(19);8312-6 [PubMed:1656436] [DOI]
- ↑ Wyndham RC, Nakatsu C, Peel M, Cashore A, Ng J, Szilagyi F . Distribution of the catabolic transposon Tn5271 in a groundwater bioremediation system. - Appl Environ Microbiol: 1994 Jan, 60(1);86-93 [PubMed:8117095] [DOI]
- ↑ Lima-Mendez G, Oliveira Alvarenga D, Ross K, Hallet B, Van Melderen L, Varani AM, Chandler M . Toxin-Antitoxin Gene Pairs Found in Tn3 Family Transposons Appear To Be an Integral Part of the Transposition Module. - mBio: 2020 Mar 31, 11(2); [PubMed:32234815] [DOI]