Mobile element type:
Transposon
Name:
TnTsp1
Synonyms:
Tn7204
Accession:
TnTsp1-NC_014154
Family:
Tn3
Group:
Tn4651
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Thiomonas intermediaK12
Date of Isolation:
2010
Country:
Germany
Molecular Source:
plasmid pTINT01
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGCTGGTC TGTCGTAGCT TTCCAAAGGA CAGCAGGT |
| IRR | 4538-4575 | - | 38 | GGGGTAGAGC AAGCAGTCGT GTCACCAGCC GCCATTTC |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | TnTsp1 | 836-966 | + | 131 | GCCTGTCGGA AAACATTTGA TTGTCGACAG ATCTTCAACA GCCCTCTGCA CCAAGCTCCG AGTGGCCGCA AAATTGTGCG GAAAACTGTG TCGCCAGACG CTACCATGCG GAAACCGCGT CTTGATGGTT T |
| res_site_I | TnTsp1 | 836-864 | + | 29 | GCCTGTCGGA AAACATTTGA TTGTCGACA |
| res_site_II | TnTsp1 | 898-941 | + | 44 | TGGCCGCAAA ATTGTGCGGA AAACTGTGTC GCCAGACGCT ACCA |
| res_site_III | TnTsp1 | 942-966 | + | 25 | TGCGGAAACC GCGTCTTGAT GGTTT |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| parE | TnTsp1 | 176-415 | - | Passenger Gene | Toxin |
| parD | TnTsp1 | 457-735 | - | Passenger Gene | Antitoxin |
| tnpR | TnTsp1 | 989-1564 | + | Accessory Gene | Resolvase |
| tnpA | TnTsp1 | 1569-4535 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | parE | ParE | TnTsp1 | 79 | 176-415 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Toxin | ||||||||||
| Sequence Family: | ParE_toxin (Pfam:PF05016) | ||||||||||
| Target: | DNA gyrase | ||||||||||
| Protein Sequence: | MWDYIAADNP RAAARMDEIF SDAAARLIQH PMLGKPGKIP GTRELIPHES YRLVYQIDGE TVWILTLVHT ARLWPPVRD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | parD | ParD | TnTsp1 | 92 | 457-735 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antitoxin | ||||||||||
| Sequence Family: | parD (PDB:4Q2U) | ||||||||||
| Comment: | RelB/ParD/CcdA/DinJ | ||||||||||
| Protein Sequence: | MSKQAVFTMK LEPELRAEFM AEAEAAHRPA SQVLRELMRE FVQRQRESRE YDEFLRRKVE AGRASMRAGL GRSNDEVEAE FAARRASVAR QS |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | TnTsp1 | 191 | 989-1564 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MRVSSDSDRQ STDLQRDALL AAGVDPRHLF EDYASGAKDD RAGLVQALEF VRPGDVLVVW KLDRLGRSLS HLLTIVTSLK DKRVAFRSLT ENLDTTTPSG EFLFQVFGAL AQYERALIQE RVVAGLAAAR KRGRIGGRPQ AITGEKLDAI IAALEGGMSK AAVCRNFGVK RTTLIETLTR AGWPASVPPG A |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | TnTsp1 | 988 | 1569-4535 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MADGGGSKED QWVLAPAERE LVMTKNRANR LGFAILLTFF RDRGRFPRDE TEVEVQGIAA LAKQLDAPAP IDGEAFLTGR TAERLRGEIR VRFGFREATV ADAEMLTEWL RDHVAGEVGG DIEPMIERLE GRCRELAIEP PKPDRMERIA RSALRSHEDR FHSCVYGRLP PATRERLDAL LRPEESGHGE SAVEDAQGEA AGNAPAVLLK LRGSPGRPSL ASMQDELAKL ELIRGIELSA DLFDRTSPRD LERCRQRVSV EVPRDLRRHP DAARLTWLAA FVHLRARSLT DDLVDLLIET IHQIGARAER KVERELLEDL KRVSGKQNLL FNLADATLAQ PDGVVRDVVF PVVGEQTLRD LVKEWKATGP TYRITLRTVI RNSYQGHYRR IVPTLLAALV FRSNNDRHRP VMDALALVKR FADTKVHTFP ADIEVPLDGV VRGLWREAVM ETDAAGRDRV NRVTYEIAVL EALRERLRCK EIWVVGANRY RNPDDDLPAD FEQNREDYYR ALNLPLDVER FIADLQAEMR AALSTFDAGL KKNPSVRLSS KGGGWITLTP LDAQPDPPNL TALKAELNVL WPMTSLLDMV KETDLRLSFT DALKSPTSYE SMDRSVLQPR LLLCLHGLGT NAGLQRMAGL DSGTTARDLA YVRRRYISVD AMRRAIAIVA DGTLQARNPA IWGSGTTACA SDSKHFGAWD QNLTTQWHVR YGGRGVMIYW HVERSSLCIH SQLKSPSSSE VASMIEGVIH HCTEMEVDRQ YVDSHGQSTV AFAFCRLLGF QLLPRLKAIH SQKLYRPETG KADVYANLQQ ILTKPIDWDS VRQQYDQMVK YATALRLGTA DTEAILRRFT KKNVQHPTYK AFAELGKAIK TIFLCRYLHD EALRREINEG LNVVEQWNGA TDFVFFARRG EMASNRREDH EVSMLALHLI QNCMVYINTL MIQKVLALPH WQGRFTPRDY AALTPLIWEH VNPYGRFDLD MNTRLDLP |
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