Mobile element type:
Transposon
Name:
TnTin1
Synonyms:
Tn7203
Accession:
TnTin1-CP002021
Family:
Tn3
Group:
Tn4651
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Thiomonas intermedia K12
Date of Isolation:
2010
Country:
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-32 | + | 32 | GAGGGTCGGC AGGGATTCAT GTAAAAAACC GC |
| IRR | 6092-6123 | - | 32 | GAGGGTCGGC AGGGATTCAT GTAAAAAACC GC |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res_acc_IR1 | TnTin1 | 1168-1176 | + | 9 | TAGTATGTA |
| res_acc_IR1 | TnTin1 | 1186-1194 | - | 9 | TAGTATGTA |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpT | TnTin1 | 43-1143 | - | Accessory Gene | Resolvase |
| istA | ISThsp2 | 1519-3048 | + | Transposase | None |
| istB | ISThsp2 | 3041-3859 | + | Accessory Gene | ATPase Transposition Helper |
| tnpA C-ter | TnTin1 | 4054-6075 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpT | TnpT | TnTin1 | 366 | 43-1143 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Comment: | enhances recombination (resolution)||integrase-like protein | ||||||||||
| Protein Sequence: | MARAGIYKSE VLRARDKLLV MGRNPSIDAV REELGTGSKS TIHRYLKEIE EEEGGATGTR IAVSEAIQDL VGRLAARVNE EAEERVTAAQ ARHAEQLSQQ QQAAAALKTE AQSTRQQLEH TQRILAEEKT AHGKTSEALS RKTLECTQVA QQVADLQERL AAEERHRQSL EEKHQHARQA LEHFRESTKE QRDQDQRKHE QQVQYLQAEL RSVNETLATK QQQAVHTLQD NARLLGDLSR AQGDLHQVQE EVRGLRPLQD ELGFAQRRTE ELGRRLVEQE AAVQQLNASN EQLQAKVNDL ITAKQQLELA LAAAKSSVAA QEQVVASMLE RFSATVPSPD ASAKDPIEGA EKSAKRNEKK PDATRT |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | istA | IstA | ISThsp2 | 509 | 1519-3048 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | VITMDMIGKI RRMHFRQNKS VREIARSTGL SRNTVRTWLR QPGDVVPRYA ARRTQPSRKL APYVEWLRQA VAIDAQRPKA QRRTARALHA ELKRQGYDGA YSRVTDLLRA WPQADGTSAV HAFVPLTFAL GEAFQFDWSE ESMVVGGVPY RVQVAHVKLC ASRAFWLGAY PSQGHEMLFD AHTQAFAAFG GVPHRGIYDN MKTAVDKVHP RKKRDVNARF AAMCSHYLFD PDFCNVASGW EKGVVEKNVQ DSRRRVWIEA LSTPWRSFAE LNAWLAMRCR GLWQEIFHPE FSQFTVAEML EHEQPHLMPM PTAFDGYVDN TVKVSSTCLV AMAGNRYSVP CELAGRRVST RVYPTEVVVV HDGVCVARHA RLANRGQTIY DWQHYVPLIQ RKPGALRNGA PFADLPQPLQ QLRQALLRQD GGDRLMAQVL ALVPASGLEA VLVAAALVLE ATAPSGRISV EHVVNVMGRL QTGPQPAQVT TALTMADPPR ADTARYDRLR VTDEERDHA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | istB | IstB | ISThsp2 | 272 | 3041-3859 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | ATPase Transposition Helper | ||||||||||
| Comment: | ATPase | ||||||||||
| Protein Sequence: | MREVIAELKA LRLHGMAGAW ADLQGLGTNA RLDAAQWLVE HLLQAEQEDR AVRSVRHQIL SARFPVHRDL AGFDFDASRV DRTLVGQLAS MAFTEATHNV VLVGGPGTGK SHLGTAIGVA GITQHGKRVR FYSTVDLVNA LEQEKAQGKA GRIAASLLRM DWVILYELGY LPFSQAGGAL LFHLLSKLYE HTSVLITTNL AFGEWSSVFI DAKMTTALLD RLTHHCHILE TGNESYRFRH STETAKTRIK AREQRRSGTA QAPQSAEPET PL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA C-ter | TnpA C-ter | TnTin1 | 673 | 4054-6075 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | VGQLKDILSA YQLDGTDTQR VEAISTTLVA EVDELLNECE QHLAYAGRNH LPFLLQPYKM VRAQLLNCID IASPKASSED LVVERLMEAL SKLRDNRADT VPLDMLGLSE DTDLRWMSAQ WRKLVLVKPA GKGRAESVRR RYFELAVMHA VKDDLKSGDL FIKFGERYDD YREQLVDDET FERELGDYGQ VTGIETEPGA FVSKLKSFMA LRAMEIDAGF PENAHAEIVD GRLILRKPPR SDIVEAAAHI DGMITERMEA ASIVDVMIDT ERWLDLHKLF RPLAGTDSRL EDLRMRVITT LFCYGCNLGP VQTAKSIKGL SRRQISWLNL KYVSEDLLDK AIVKVINAYN KFELPGYWGT GKHASADGTK WNLYEQNLLS EHHIRYGGYG GIGYYHVSDK FIALFSHFIS CGTYEGIHIL DGLMSNESDI RPDTIHGDTQ AQSYPVFALA HLLGIQLMPR IRGIQDLKFH RPQAGTVYQN INALFSDVID WQLIELHLPA MLRVAVSIKT GKITPSAILR RLGTYSRKNK LYFAFVELGK VIRTMFLLSY IGDVGLRKVI HAETNKSEQF NGFAGWSFFG GEGIIAENIR HEQRKVIKYN HLVANMIILH NVVGMTRVLR ELRDEGTEIT PEILGGLAPF RTAHINRFGD YTLDFRRKIG PLDFEATIIP MES |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| ISThsp2-MH257753 | ISThsp2 | insertion sequence | 1413-4053 | + | 2641 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRL | ISThsp2 | 1413-1440 | + | 28 | TGTTCGTGCC GAACGAAAAT TGACCCAC |
| IRR | ISThsp2 | 4026-4053 | - | 28 | TGTTCGTGCC GATGGAATTT TGACCCAC |