Mobile element type:
Transposon
Name:
TnThsp9
Synonyms:
Tn7202
Accession:
TnThsp9-FP475957.1
Family:
Tn3
Group:
Tn3
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Thiomonas sp. str. 3As
Date of Isolation:
2010
Country:
Molecular Source:
plasmid pTHI
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-47 | + | 47 | GGGGTTCGGG GAGCAATGGA ACAGCCAACC GACTTAAGCG CTCGGCA |
| IRR | 6171-6215 | - | 45 | GGGGTTCGGG GAGCAATGGA ACAGCCAACC GACTTAAGCC CTGGC |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | TnThsp9 | 975-1111 | + | 137 | AGTCTGCTTT ACTCGTTGAA AGAGAATAAT ATCGAACTTT GATTCATCAA ACCACAAAGT TAGACGAATT GCGGTGCTTC ACCGTTTGCC CGTCAGGTCG CGCGGCAGAG TCTTGTAAAA CGAAGGTTTT ATCGAAC |
| res_site_III | TnThsp9 | 975-1004 | + | 30 | AGTCTGCTTT ACTCGTTGAA AGAGAATAAT |
| res_site_II | TnThsp9 | 1009-1048 | + | 40 | AACTTTGATT CATCAAACCA CAAAGTTAGA CGAATTGCGG |
| res_site_I | TnThsp9 | 1084-1111 | + | 28 | GTCTTGTAAA ACGAAGGTTT TATCGAAC |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpR | TnThsp9 | 48-968 | - | Accessory Gene | Resolvase |
| RHH_6 | TnThsp9 | 1148-1417 | + | Passenger Gene | Antitoxin |
| PIN_3 | TnThsp9 | 1417-1836 | + | Passenger Gene | Toxin |
| tnpA | TnThsp9 | 1840-4812 | + | Transposase | |
| THI_p0011 | TnThsp9 | 4825-5034 | - | Passenger Gene | Hypothetical |
| merP | TnThsp9 | 5042-5317 | - | Passenger Gene | Heavy Metal Resistance |
| merT | TnThsp9 | 5330-5677 | - | Passenger Gene | Heavy Metal Resistance |
| merR | TnThsp9 | 5753-6151 | + | Passenger Gene | Heavy Metal Resistance |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | TnThsp9 | 306 | 48-968 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MKIGYARVST RDQKSDLQVD ALKAAGCERV YQDVASGAKT ARPALDELLG QLRAGDVLVI WKLDRMGRSL KHLVELVGSL MERKVGLLSL NDPIDTTSAQ GRLVFNLFAS LAEFERELIR ERTQAGLTAA RARGRVGGRP KGLTPQAEAT ALAADTLYRE RKLSVAAIAQ KLHVSKSTLY SYLRHRGVEI GPYKKPARGQ PGPSTSAGEA DPPKVATIRL TLRIENNSKF VRGKKRARED IERYCLSEYD GQRLPSGEYE LKVPYSSDDD LDKRMHELLG DISSEADDRN CFSESDARMD GTDRYW |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | RHH_6 | RHH_6 | TnThsp9 | 89 | 1148-1417 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antitoxin | ||||||||||
| Sequence Family: | RHH_6 (Pfam:PF16762) | ||||||||||
| Protein Sequence: | MHPNTVLEGV MNTVRWNIAV SPDVDQSVRM FIAAQGGGRK GDLSRFIEEA VRAYLLERAV DQAKTAAASM SETELTDLID EAVQWAREH |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | PIN_3 | PIN_3 | TnThsp9 | 139 | 1417-1836 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Toxin | ||||||||||
| Function: | 18 : Unknown function | ||||||||||
| Sequence Family: | PIN_3 (Pfam:PF13470) | ||||||||||
| Target: | single stranded RNA | ||||||||||
| Comment: | tRNA(fMet)-specific endonuclease | ||||||||||
| Protein Sequence: | MRVILDTNVL LGALISPHGP PDAIYRAWRA ARFELVTSAA QLDELRRVSR YPKLKTILPA HRVGTMVNNM QRAIVLAQLP PLPDGIEAND PNDAFLLAMA LGGEADYLVT GDRRAGLLQR GSIGRTRIVT PATFCAEAL |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | TnThsp9 | 990 | 1840-4812 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPVSFLSTTQ RERYGRYPET LSSEELARYF HLDDDDREWI ATKRRDSHRL GYALQLTTAR FLGTFLEDPA AVPGAVLHTL SSQLGIADPD CVLAYRESEQ RWRHTTEIRA RYGYREFADG GVQFRLGRWL CALCWTGTDR PSALFDYANG WLVGHKVLLP GVTVLERFIA EVRSRMESRL WRLLVRGVTA AQRQRLDDLL KPAEGSRQSW LDRLRKGPVR VSAPALVLAL LRIETVRGLG IRLPGTHVPP SRIAALARFA STVKVSAVAR LPEARRIATL VAFVHCLEAS AQDDALDVLD LLLRELFTKA EKEDRKVRQR SLKDLDRAAS TLAEACRMLL DPALPDGELR ERVYAAIGRD ELAQALNEVR GLVRPPNDVF YTELEARKAT VSRFLPTLLR VIRFDANPAA QPLAQALQWL HEKPDHDPPT AIVGKAWQRH VVQDDGRINA TAYSFCALDK LRSAIRRRDV FISPSWRYAD PRAGLLAGAE WEAARPIVCR SLGLTAQPEA TLSALTRELD ETYRRVAARL PENDAVRFET VGDKTELVLS PLEALEEPAS LIALRNEIKA RMPRVDLPEI LLEVAARTGC MEAFTHLTER TARAADLTTS LCAVLMAEAC NTGPEPLVRQ DSPALRRDRL MWVDQNYVRD DTLIACNAVL VAAQNRIALA RAWGGGDVAS ADGMRFVVPV RTIHAAPNPK YFNRGRGVTW YNLLSDQCTG LNAITVPGTL RDSLVLLAVV LEQQTELQPT QIMTDTGAYS DVVFGLFRLS GYRFCPRLAD VGGTRFWRVD AEADYGDLNT LARQRVNLDR ITPHWDDVLR LVGSLKLGMV PAMGIMRTLQ VDERPTSLAQ AIAEIGRIDK TIHTLNFIDD EARRRATLLQ LNLGEGRHSL AREVFHGKRG ELFQRYREGQ EDQLSALGLV VNMIVLWNTL YMDAVLAQLR SEGYRVKPED EARLSPFGHE HINMLGRYSF SVPEAVARGE LRPLSRKDDV |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | THI_p0011 | THI_p0011 | TnThsp9 | 69 | 4825-5034 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Function: | 18 : Unknown function | ||||||||||
| Comment: | part of mercury operon | ||||||||||
| Protein Sequence: | MTIILESVLT CPHCGHAWQK TMPTDACQFY DECPACHALL RPKPGDCCVF CSFGSVPCPP IQQQQGCCG |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | TnThsp9 | 91 | 5042-5317 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | Periplasmic mercury ion-binding protein | ||||||||||
| Protein Sequence: | MRKLLIALLV ALPFTVLAAP SKTVTLAVQN MTCPLCPITV KKSLEKVAGV SAVQVDFDKK TATVTYDPDK AQPEALTKAT TNAGYPSAVQ K |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | TnThsp9 | 115 | 5330-5677 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | mercury transporter | ||||||||||
| Protein Sequence: | MASLTGKSSL VAGILAAIGA SVCCVGPLVL LALGIGGTWV GSLTAMEPYR PFFIGLTLLF LGLAFRKLYL VQQVCTPGTP CADPRTRRRQ RLTFWIVAIL LLALLAVPWL APLFY |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR | MerR | TnThsp9 | 132 | 5753-6151 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MGADLTIGKL ADAAGVNIET IRYYQRRGLL DEPAKPLGGH RRYPAGEAKR VRFIKRAQAL GFTLDEVGML LTLDSACGCS DTRALAARKQ GLIERKMADL TAMYQALGDL IQQCDTGGST RPCPIIDVLE RD |
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