Mobile element type:
Transposon
Name:
TnSsu5
Synonyms:
Tn7232
Accession:
TnSsu5-KP998101.1
Family:
Compound Transposon
Group:
ISSsu5
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Staphylococcus aureus LAR2682
Date of Isolation:
2018
Country:
Greece
Molecular Source:
chromosome
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnp | ISSsu5 | 159-1478 | + | Transposase | |
| lsaB (ARO:3003111) | TnSsu5 | 2079-3563 | + | Passenger Gene | Antibiotic Resistance |
| lnuB (ARO:3002836) | TnSsu5 | 3617-4420 | + | Passenger Gene | Antibiotic Resistance |
| recombinase | TnSsu5 | 5261-5929 | + | Passenger Gene | Other |
| HTH transcriptional regulator | TnSsu5 | 6072-6296 | + | Passenger Gene | Other |
| nucleotidyltransferase | TnSsu5 | 6311-7180 | + | Passenger Gene | Other |
| methyltransferase | TnSsu5 | 7161-7895 | + | Passenger Gene | Other |
| ANT(6)-Ia (ARO:3002626) | TnSsu5 | 7927-8790 | + | Passenger Gene | Antibiotic Resistance |
| adenine phosphoribosyltransferase | TnSsu5 | 8834-9361 | + | Passenger Gene | Other |
| tnp | ISSsu5 | 9589-10908 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | ISSsu5 | 439 | 159-1478 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MTSLHKNQVK FNSNITISHT GGRLSSDSGL VLVKELMNTF GFSHLAKQWL HITDHRAYFT HDNLAILEQL IMQLIAGYSA DSSANLLRRD PVFQAVLGKK QLASQSSISR FLDRFTEENI DQLQALNQSL IDKARLIRND TELIIDVDST HSDTFGRQEQ TDYNAHYQTY GYHPLVAFDG LTGDFLKAEL RSGNQYTSKG VKAFINPLLH HYKSTLPHTE ILVRGDSGFA TPEVYESCEA TESQYVIRLK SNRRLSQLAE HSVLYGDNQE WEEREVQYFS LPYQAQSWSK PRRVCIRSVR EGGELLFHHA FIVTNLSDNV SPEVIFSLYG KRGTMENFIK EAKGGFYFDK TDSPRFLENH VRMMISVLAY NLVNFLKSIG FDKVNQGMTI HSIRLILLKV AGKLVQTGRQ VYLKLSSYHV YQTEFYKVFE RLRRSKQWI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | lsaB (ARO:3003111) | LsaB | TnSsu5 | 494 | 2079-3563 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target protection (ARO:0001003) | ||||||||||
| Sequence Family: | lsa-typeABC-F protein (ARO:3004472) | ||||||||||
| Target: | lincosamide antibiotic (ARO:0000017)||pleuromutilin antibiotic (ARO:3000670)||streptogramin antibiotic (ARO:0000026) | ||||||||||
| Comment: | loose match to reference sequence for ARO:3003111 (bitscore: 625)||confers pristinamycin, virginiamycin, quinupristin, and dalfopristin resistance | ||||||||||
| Protein Sequence: | MSLINVSNLT FSYEGSYDNI FENVSFQIDT DWKLGFIGRN GRGKTTFLNL LLGKYAYSGN ISSTVKFEYF PYDVEDKSLY TIEVMKSICT EFMDWEIFRE ISLLDVQEDA LYRPFNTLSN GEQTKVLLAA LFLTASCFLL IDEPTNHLDI DARNVVQNYL KRKKGFILVS HDRSLLDQCV DHILSINKTN IEIQKGNFTS WWENKTLQDN FELAENKKLL KEIGRLSYAA KRSSNWSNKV EKSKYGTTNS GSKLDKGYVG HKAAKAMKRA KNIESRHQEA VLQKSELLHN IEQYDDLKIS PLEFHKECLI EANDLSLSYG DKEVCSNLNF RVNIGDRVAI IGKNGSGKSS ILKLINGDDI KFTGNFMLAS GLKISYISQD TSYLKGNLSE FAYNNKIDET LFKTILRKLD FNREQFDKNM VDFSAGQKKK VLIAKSLCES AHLYIWDEPL NYIDIFSRIQ IEKMILEYCP TLLFVEHDDA FCNNICTKNI NLGL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | lnuB (ARO:3002836) | LnuB | TnSsu5 | 267 | 3617-4420 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | lincosamide nucleotidyltransferase (LNU) (ARO:3000221) | ||||||||||
| Target: | lincosamide antibiotic (ARO:0000017) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002836||Synonyms: linB | ||||||||||
| Protein Sequence: | MLKQKELIAN VKNLTESDER ITACMMYGSF TKGEGDQYSD IEFYIFLKDS ITSNFDSSNW LFDVAPYLML YKNEYGTEVV IFDNLIRGEF HFLSEKDMNI IPSFKDSGYI PDTKAMLIYD ETGQLENYLS EISGARPNRL TEENANFLLC NFSNLWLMGI NVLKRGEYAR SLELLSQLQK NTLQLIRMAE KNADNWLNMS KNLEKEISLE NYKKFAKTTA RLDKVELFEA YKNSLLLVMD LQSHLIEQYN LKVTHDILER LLNYISE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | recombinase | Recombinase | TnSsu5 | 222 | 5261-5929 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Sequence Family: | Zn_ribbon_recom (PF13408) | ||||||||||
| Protein Sequence: | MQAQEEIYRR SNIYTGEGKN KRIYSSKYAL SAITFCGYCG DIYRRTYWNI HGRKEFVWRC VTRIEQGPEV CKNRTVKEDE LYGAVMTAIN KLLAGGNNMI KTLEENIHAV IGETTEYQIS EINTLLDEKQ KELIKLANKG QDYEYLVDEI DEMRDKRQTL LVEDASLSGE NERINELIEF IRKNKFRTLE YDDKLVRKII QNVKVYEDHF VIAFKPGIEM EI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | HTH transcriptional regulator | HTH transcriptional regulator | TnSsu5 | 74 | 6072-6296 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Sequence Family: | HTH_26 (Pfam:PF13443) | ||||||||||
| Protein Sequence: | MTTAEMIKEL CEQMNISVSE LARRIGQTPQ NFNKKLQRET VTLDELKAIA DVLGVKFVQA FILPDGDEIK ISNE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | nucleotidyltransferase | Nucleotidyltransferase | TnSsu5 | 289 | 6311-7180 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Sequence Family: | NTP_transf_2 (PF01909) | ||||||||||
| Protein Sequence: | MVGNVIQIVT EKLSSLPFIE GIVLGGSRAR STHTENSDID IGIYYNSDSF DLTAINQIAT ELDDENRNNL VVPPGAWGDW VNGGGWLVIN GCHVDLILRD IKRVEQIIKD TEQGIVTANY QTGHPHGYIS AMYRGELAIS KILYAKNESL CELKKQAEIY PTALKKSLMN FFIFEAEFSL MFVKANAGAE DKYYIAGHVF RIISCLNQVL FACNNAYCIN EKKAIKLLET FEYKPEKYAE RVNHIFEVLG LSLFECYDMT EKLYKEVNEI VSEINNFLNE ESSDERKQI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | methyltransferase | Methyltransferase | TnSsu5 | 244 | 7161-7895 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Sequence Family: | Methyltransf_11 (PF08241) | ||||||||||
| Protein Sequence: | MKENKYDDNI FFQKYSQMSR SQKGLAGAGE WETLKKMLPD FKGKRVLDLG CGYGWHCIYA MENGASSVVG VDISHKMLEV AKGKTHFPQI EYECCAIEDV DFPEESFDVI LSSLAFHYVA DYENLIKKIY RMLKAGGNLV FTVEHPVFTA HGTQDWYYNE KGEILHFPVD NYYYEGKRTA MFLEEKVTKY HRTLTTYLNT LLSNSFIINQ IVEPQPPENM MDIPGMADEM RRPMMLIVSA KKKM |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | ANT(6)-Ia (ARO:3002626) | ANT(6)-Ia | TnSsu5 | 287 | 7927-8790 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | ANT(6) (ARO:3000225) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002626||Synonyms: ant6, aadE | ||||||||||
| Protein Sequence: | MRSEKEMMDL VLSLAEQDER IRIVTLEGSR ANINIPKDEF QDYDITYFVS DIEPFISNDD WLNQFGNIIM MQKPEDMELF PPEEKGFSYL MLFDDYNKID LTLLPLEELD NYLKGDKLIK VLIDKDCRIK RDIVPTDIDY HVRKPSAREY DDCCNEFWNV TPYVIKGLCR KEILFAIDHL NQILRFELLR MMSWKVGIKT EFSLSVGKNY KYINKYIDED LWNRLLSTYR MDSYENIWKS LFICHQLFRE VSKEVAELLG FDYPEYGKNI TRYTEDMYKK YVENDYF |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | adenine phosphoribosyltransferase | Adenine phosphoribosyltransferase | TnSsu5 | 175 | 8834-9361 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Sequence Family: | Pribosyltran (PF00156) | ||||||||||
| Protein Sequence: | MESTDVINEI RGILPRNSIG KYDLLTIFTH KTVFDKIVKV LSLDFQNKVD YVAAPEAIGW ILGTAIAKEL GVGFIGVRKG DKLPYAKEEI ISTHFTDYSG QDKSFEISKS SIVRNKRVLI VDDWIETGSQ MKALIALLEK LDCSIIGLAT IGIDINEVTQ KWIDSSFVAY IGSNI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | ISSsu5 | 439 | 9589-10908 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MTSLHKNQVK FNSNITISHT GGRLSSDSGL VLVKELMNTF GFSHLAKQWL HITDHRAYFT HDNLAILEQL IMQLIAGYSA DSSANLLRRD PVFQAVLGKK QLASQSSISR FLDRFTEENI DQLQALNQSL IDKARLIRND TELIIDVDST HSDTFGRQEQ TDYNAHYQTY GYHPLVAFDG LTGDFLKAEL RSGNQYTSKG VKAFINPLLH HYKSTLPHTE ILVRGDSGFA TPEVYESCEA TESQYVIRLK SNRRLSQLAE HSVLYGDNQE WEEREVQYFS LPYQAQSWSK PRRVCIRSVR EGGELLFHHA FIVTNLSDNV SPEVIFSLYG KRGTMENFIK EAKGGFYFDK TDSPRFLENH VRMMISVLAY NLVNFLKSIG FDKVNQGMTI HSIRLILLKV AGKLVQTGRQ VYLKLSSYHV YQTEFYKVFE RLRRSKQWI |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| ISSsu5-NC_009442 | ISSsu5 | insertion sequence | 1-1683 | + | 1683 |
| ISSsu5-NC_009442 | ISSsu5 | insertion sequence | 9431-11113 | + | 1683 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRL | ISSsu5 | 1-29 | + | 29 | CCCTGAATAA TTCATACTTT TTAATTGAA |
| IRR | ISSsu5 | 1655-1683 | - | 29 | CCCTGAATAA TTCATAGCTC TAAATTTAA |
| IRL | ISSsu5 | 9431-9459 | + | 29 | CCCTGAATAA TTCATACTTT TTAATTGAA |
| IRR | ISSsu5 | 11085-11113 | - | 29 | CCCTGAATAA TTCATAGCTC TAAATTTAA |
1. Sarrou S, Liakopoulos A, Chasioti M, Foka A, Fthenakis G, Billinis C, Spyrou V, Pantelidi K, Roussaki-Schulze A, Lachanas V, Makaritsis K, Skoulakis C, Daikos GL, Dalekos G, Spiliopoulou I, Petinaki E.
Dissemination of Methicillin-Susceptible CC398 Staphylococcus aureus strains in a rural Greek area.. PLoS One. 2015 Apr 2;10(4):e0122761. doi: 10.1371/journal.pone.0122761. eCollection 2015. PubMed ID:
25835293
.
2. Sarrou S, Liakopoulos A, Tsoumani K, Sagri E, Mathiopoulos KD, Tzouvelekis LS, Miriagou V, Petinaki E. Characterization of a Novel lsa(E)- and lnu(B)-Carrying Structure Located in the Chromosome of a Staphylococcus aureus Sequence Type 398 Strain.. Antimicrob Agents Chemother. 2015 Nov 23;60(2):1164-6. doi: 10.1128/AAC.01178-15. Print 2016 Feb. PubMed ID: 26596938 .
2. Sarrou S, Liakopoulos A, Tsoumani K, Sagri E, Mathiopoulos KD, Tzouvelekis LS, Miriagou V, Petinaki E. Characterization of a Novel lsa(E)- and lnu(B)-Carrying Structure Located in the Chromosome of a Staphylococcus aureus Sequence Type 398 Strain.. Antimicrob Agents Chemother. 2015 Nov 23;60(2):1164-6. doi: 10.1128/AAC.01178-15. Print 2016 Feb. PubMed ID: 26596938 .