Mobile element type:
Transposon
Name:
TnSku1
Synonyms:
Tn7197
Accession:
TnSku1-CP002358.1
Family:
Tn3
Group:
Tn4430
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Sulfuricurvum kujiense DSM 16994
Date of Isolation:
2006
Country:
Japan
Molecular Source:
plasmid pSULKU03
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGTCACGT GCCGAAACGG ATAGAATCGT ACGCTAAG |
| IRR | 4447-4484 | - | 38 | GGGGTCACGT GCCGAAACGG ATAGAATCGT ACGCTAAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| HTH | TnSku1 | 126-416 | + | Passenger Gene | Antitoxin |
| Gp49 | TnSku1 | 419-712 | + | Passenger Gene | Toxin |
| tnpR | TnSku1 | 713-1336 | - | Accessory Gene | Resolvase |
| tnpA | TnSku1 | 1492-4410 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | HTH | HTH | TnSku1 | 96 | 126-416 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antitoxin | ||||||||||
| Sequence Family: | HTH (PDB:5TN0) | ||||||||||
| Protein Sequence: | MYNIQQTQKF SQWLIKLKDM RARIAIARRI ERAQCGNLGD AKSVGESVYE MRIDMGPGYR LYYTMRGDEI IVLLVGGDKS TQQRDIEKAI EMAKEI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | Gp49 | Gp49 | TnSku1 | 97 | 419-712 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Toxin | ||||||||||
| Sequence Family: | Gp49 (Pfam:PF05973) | ||||||||||
| Target: | ribosome associated mRNA | ||||||||||
| Protein Sequence: | MELTLTPFDM TQYLDSEEAI AEYLTQVLEE GDTDELIRAI GHIAKARGMS QIAQESGLGR ESLYKTFAPG SKPRFDTIAK VVHSLGLHFQ ITPVRAS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | TnSku1 | 207 | 713-1336 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MLIGYARVSK ADGNQVLDLQ IDALQGIGIT YGQIYVDKAS GAKDDRPGLD NCLKALREGD TLVVWKLDRL GRNLKHLVST VDDLSRRNIG FKVLSGQGAD IDTTTPAGKM VFGIFAALAE FERELIRERT IAGLNAARAR GRKGGRKFQL TKAQIRLAQA AMSNRDTSVS ELCKEIGVTR ATLYRYVGPT GELREFGKKV LGIEETP |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | TnSku1 | 972 | 1492-4410 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPRKKILSSL QREMLEKLPE QFEELAKHYL LTSDDLEFIS QRRRSSNKLG FALSLCMMRY PGRTLKIDEI PPKALVEFIA EQLGLNYEDF LGYALRAETR REHQYLLMQK YGYKNFNDRY LKEMVRWLTP IAIENHKGIF LLQTLMHEMR YRKILLPSLL QIESMVSLSL SRAEKIIQQT VTSFLNKKNM QLLDDLLAFD VSVGQSIFSW LRQSESRPTS ANMLVIMERL QTIKRLEISE TISSRVSQIR MQQYSREGYR ISISHLRQYE PKRRYTLIAI TLLEIQKALI DDAITMHDRI IGSLMRRSQN RFHQTIQDGD KKAKELIRFF SRTSQLLIES RNNGANPWES IDLHIGWDSF LQSVQEATSL SEPIKQDHLA FIETHYVQMR KYTSRFLEEF DFKASAGGGD VMDAIRLLQE LNSKNKRKLP EDAPVTFIPS RWKPFIYTEE GMDRRYYELC ALSELKNRLR SGDIWIEGSK QYLDFDSYLM TRHSYSEMKS QNNLPIAVEI DFSRYLINRQ KQLGESIDEL SDMIKDGTIA DVEFTQNGYS IKPYRTDGAP KEATNIIRYV ANLLPRIKIT DLLIEVDTWT KFSDQFTHLR SGMTSTDKKA LFAAILSDGI NLGHQKMADA CADISVGRLR SASDWYIRDE NYSSALAQIT NHHHQTSLVK NWGDGTTSSS DGQRFPTGGT GDALGGYNAK YGTQPGVVFY THISDQYSPF HTQIIHANAR DATYVLDGLL YHESDLKIEE HYTDTAGFTD HVFALCHLLG FKFAPRIRDI ADTKLFALEG VEVPEKFNNI ITGSLKIKDM QQNWDDVLRL AASIKIGTAS ASLLIRKLAS YPRQNRLALA IRDVGRIERT LFILEWLKNP SLRSRVQIGL NKGEARNSLA RTVFFNRLGE VRDRTFENQL YRASGLNLIV AAIILWNTVY LEKAIEQAKK EIEIPDEFLK YFSPLGWEHI NLTGDYVWRM EP |
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1. Han C, Kotsyurbenko O, Chertkov O, Held B, Lapidus A, Nolan M, Lucas S, Hammon N, Deshpande S, Cheng JF, Tapia R, Goodwin LA, Pitluck S, Liolios K, Pagani I, Ivanova N, Mavromatis K, Mikhailova N, Pati A, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Brambilla EM, Rohde M, Spring S, Sikorski J, Goker M, Woyke T, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP, Detter JC.
Complete genome sequence of the sulfur compounds oxidizing chemolithoautotroph Sulfuricurvum kujiense type strain (YK-1(T)).. Stand Genomic Sci. 2012 Mar 19;6(1):94-103. doi: 10.4056/sigs.2456004. Epub 2012 Mar 5. PubMed ID:
22675602
.