Mobile element type:
Transposon
Name:
TnSen1.2
Synonyms:
Tn7192
Accession:
TnSen1.2-CP028162
Family:
Tn3
Group:
Tn21
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Pseudomonas aeruginosa strain MRSN12280
Date of Isolation:
2018
Country:
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-39 | + | 39 | GGGGTCGTCT CAGAAAACGG AAAAAATCGT ACGCTAAGC |
| IRR | 8482-8522 | - | 41 | GGGGTCGTCT CAGAAAACGG AAAAAATCGT ACGCTAAGAC C |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| msf C-ter | TnSen1.2 | 101-763 | - | Passenger Gene | Other |
| tnp | ISPa96 | 832-1794 | + | Transposase | |
| msf N-ter | TnSen1.2 | 1944-2369 | - | Passenger Gene | Other |
| MCR-5.1 (ARO:3004332) | TnSen1.2 | 2516-4159 | - | Passenger Gene | Antibiotic Resistance |
| chrB | TnSen1.2 | 4178-4720 | - | Passenger Gene | Heavy Metal Resistance |
| tnpR | TnSen1.2 | 4960-5520 | + | Accessory Gene | Resolvase |
| tnpA | TnSen1.2 | 5523-8489 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | msf C-ter | Msf C-ter | TnSen1.2 | 220 | 101-763 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | major facilitator superfamily (MFS) membrane transport proteins || movement of small solutes across cell membranes in response to chemiosmotic gradients | ||||||||||
| Protein Sequence: | LAVLAFLTLV KDPEHSPNPA LKFFSSLRGL PARFKRYLGA VGIFGIGDFS HSLLILAATT LLTPSLGVIQ AAQVAGLLYV WRNLVQVAVS YPVGVLADRV GHLPVLIAGY VLGTLTAVLT ALAFWLGIAS VPLLAGVFFI AGLYVAVQEA LESTVTAEMV QPDTLAMSYG ALGTVNGAAK FISSASVGVV WTAVSPVVGF GLAAVLMAAG TLTLLRVKGN |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | ISPa96 | 320 | 832-1794 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MKQHSLGLGM TAKRTRRREF LEEMERVVPW ADLVALVSPY LPEGRRGRPP FAPETMLRIH FMQQWFNLSD PAMEEALHDV PLFREFAGLE GWEERLPDES TILRFRHVLE QHQLAAQILK TVNDLLGAKG LLLRSGTVVD ATLIAAPSST KNASGQRDPE MKQSRKGQQW YFGMKCHIGV DADSGLVHTV RGTGGHVNDV VEAASLLHGQ ERDVFADAGY QGAGKRADAP PGVQWHIAMK PGQRRVLDPA DPIEGLTEQL ERLKARIRAK VEHPFRVVKR QFGHVKVRYR GLAKNTAQLH TLFALANLWL ARRQLLGAGA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | msf N-ter | Msf N-ter | TnSen1.2 | 142 | 1944-2369 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | major facilitator superfamily (MFS) membrane transport proteins || movement of small solutes across cell membranes in response to chemiosmotic gradients | ||||||||||
| Protein Sequence: | VLGLIEGIAD AIAAFTKMVS GYIADKLGHR KLFVMVGYGL TPLGQVLIAL AAGWPLLLLG RIVSWFGKGL RGPLRDAIVI QAVSPETRGR AFGFHRAADT VGAVIGPLLG VALLGWAQGI HWSDAAGPFR LVLWLSVIPG VL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | MCR-5.1 (ARO:3004332) | MCR-5.1 | TnSen1.2 | 547 | 2516-4159 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | MCR phosphoethanolamine transferase (ARO:3004268) | ||||||||||
| Target: | peptide antibiotic (ARO:3000053) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3004332 (bitscore: 1127)||Synonyms: | ||||||||||
| Protein Sequence: | MRLSAFITFL KMRPQVRTEF LTLFISLVFT LLCNGVFWNA LLAGRDSLTS GTWLMLLCTG LLITGLQWLL LLLVATRWSV KPLLILLAVM TPAAVYFMRN YGVYLDKAML RNLMETDVRE ASELLQWRML PYLLVAAVSV WWIARVRVLR TGWKQAVMMR SACLAGALAM ISMGLWPVMD VLIPTLRENK PLRYLITPAN YVISGIRVLT EQASSSADEA REVVAADAHR GPQEQGRRPR ALVLVVGETV RAANWGLSGY ERQTTPELAA RDVINFSDVT SCGTDTATSL PCMFSLNGRR DYDERQIRRR ESVLHVLNRS DVNILWRDNQ SGCKGVCDGL PFENLSSAGH PTLCHGERCL DEILLEGLAE KITTSRSDML IVLHMLGNHG PAYFQRYPAS YRRWSPTCDT TDLASCSHEA LVNTYDNAVL YTDHVLARTI DLLSGIRSHD TALLYVSDHG ESLGEKGLYL HGIPYVIAPD EQIKVPMIWW QSSQVYADQA CMQTHASRAP VSHDHLFHTL LGMFDVKTAA YTPELDLLAT CRKGQPQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | chrB | ChrB | TnSen1.2 | 180 | 4178-4720 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Chromate | ||||||||||
| Protein Sequence: | MNAQNWLLLT YKVPPEPAKK RIALWRKLKG MGAVYLQNGV CLLPKTDDHT RRLKIIENEI NEMAGDSVLL ETVALDRAQE DKVIARFKAD RDEEYKELLG KCADFEAEIA HETEVQHFTY AELEENDVDL KKLQSWLEKI AKLDFYGATL AAEAAERLKG CEALLDAYAQ RVFDAHDENR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | TnSen1.2 | 186 | 4960-5520 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MHGQRIGYIR VSSFDQNPER QLEHVQVDKV FTDKASGKDT QRPELDRLLA FVREGDTVVV HSMDRLARNL DDLRRLVQGL TQRGVRIAFA KEHLTFTGED SPMANLMLSV MGAFAEFERA LIRERQREGI ALAKKRGAYR GRKKALSPDR VAELRQRAAA GEKKAHLARE FGISRETLYQ YLRTDD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | TnSen1.2 | 988 | 5523-8489 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPRRSILSVA ERASLLALPD TEDELIRLYT FSEADLSLIR QRRGDANRLG VGVQLCLLRF PGQGLLPDAT VPVSLLQWIG RQLRTDPACW PLYAERDETR REHLLELRAY LGMVPFGLAH YRQAVKATTE LALQTDKGIV LAASVLDALR HRHIILPTLD VVERVCAEAI TRANRRIYEA LSELLSSGHR HRLDDLLKRR DNGKTTWLAW LRQSPVKPNS RHMLEHIERL KAWQALDLPS GIERSVHQNR LLKIAREGGQ MTPADLAKFE PQRRYATLVA LAIEGMATVT DEIIDLHDRI LGKLFNAAKN KHQQQFQASG KAINAKVRLF GRIGQALIEA KQAGRDPFAA IEAVMSWDAF AESVTEAQKL AQPEDFDFLH RIGESYATLR RYAPEFLAVL KLRAAPAAKD VLDAIDVLRG MNSDNARKVP ADAPTGFIKP RWQKLVMADA SIDRRYYELC ALSELKNSLR SGDIWVQGSR QFKDFEDYLV PPAKFASLKL ASELPLAVAT DCDQYLNERL TLLETQLATV NRMAAANDLP DAIITESGLK ITPLDAAVPD TAQALIDQTA MILPHVKITE LLLEVDEWTG FTRHFTHLKS GDLAKDKNLL LTTILADAIN LGLTKMAESC PGTTYAKLAW LQAWHTRDET YSTALAELVN AQLRHPFAGH WGDGTTSSSD GQNFRTGSKA ESTGHINPKY GSSPGRTFYT HISDQYAPFH TKVVNVGVRD STYVLDGLLY HESDLRIEEH YTDTAGFTDH VFALMHLLGF RFAPRIRDLG DTKLYIPKGE AAYDALKPMI GGTLNIKHVR AHWEEILRLA TSIKQGTVTA SLMLRKLGSY PRQNGLAVAL RELGRIERTL FILDWLQSVE LRRRVHAGLN KGEARNALAR AVFFNRLGEI RDRSFEQQRY RASGLNLVTA AIVLWNTVYL ERAANALRGH GQAVDDGLLQ YLSPLGWEHI NLTGDYLWRS SAKIGAGKFR PLRPLSGS |
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| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| ISPa96-CP028162.1 | ISPa96 | insertion sequence | 764-1942 | + | 1179 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IR | ISPa96 | 764-779 | + | 16 | GGAAGGTCTG CACAAA |
| IR | ISPa96 | 1927-1942 | - | 16 | GGAAGGTCTG CACAAA |