Mobile element type:
Transposon
Name:
TnSen1.1
Synonyms:
Tn7191
Accession:
TnSen1.1-KY807921
Family:
Tn3
Group:
Tn21
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Salmonella enterica subsp. enterica serovar Paratyphi B isolate 13-SA01718
Date of Isolation:
2017
Country:
Molecular Source:
plasmid pSE13-SA01718
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-39 | + | 39 | GGGGTCGTCT CAGAAAACGG AAAAAATCGT ACGCTAAGC |
| IRR | 7297-7337 | - | 41 | GGGGTCGTCT CAGAAAACGG AAAAAATCGT ACGCTAAGAC C |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| msf2 | TnSen1.1 | 101-778 | - | Passenger Gene | Other |
| msf | TnSen1.1 | 775-1335 | - | Passenger Gene | Other |
| MCR-5.1 (ARO:3004332) | TnSen1.1 | 1332-2975 | - | Passenger Gene | Antibiotic Resistance |
| chrB | TnSen1.1 | 2994-3536 | - | Passenger Gene | Heavy Metal Resistance |
| tnpR | TnSen1.1 | 3775-4335 | + | Accessory Gene | Resolvase |
| tnpA | TnSen1.1 | 4338-7304 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | msf2 | Msf2 | TnSen1.1 | 225 | 101-778 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | permease | ||||||||||
| Protein Sequence: | VIPGVLAVLA FLTLVKDPEH SPNPALKFFS SLRGLPARFK RYLGAVGIFG IGDFSHSLLI LAATTLLTPS LGVIQAAQVA GLLYVWRNLV QVAVSYPVGV LADRVGHLPV LIAGYVLGTL TAVLTALAFW LGIASVPLLA GVFFIAGLYV AVQEALESTV TAEMVQPDTL AMSYGALGTV NGAAKFISSA SVGVVWTAVS PVVGFGLAAV LMAAGTLTLL RVKGN |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | msf | Msf | TnSen1.1 | 186 | 775-1335 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | permease | ||||||||||
| Protein Sequence: | MTTSTDDPPK RWLNRTVAGA GITSSLGDFC YETTTVILPG FLAVLGIPAA VLGLIEGIAD AIAAFTKMVS GYIADKLGHR KLFVMVGYGL TPLGQVLIAL AAGWPLLLLG RIVSWFGKGL RGPLRDAIVI QAVSPETRGR AFGFHRAADT VGAVSAPCWA SRCWVGRKAF IGATRLDPSG SYYGCR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | MCR-5.1 (ARO:3004332) | MCR-5.1 | TnSen1.1 | 547 | 1332-2975 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | MCR phosphoethanolamine transferase (ARO:3004268) | ||||||||||
| Target: | peptide antibiotic (ARO:3000053) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3004332 (bitscore: 1127)||Synonyms: | ||||||||||
| Protein Sequence: | MRLSAFITFL KMRPQVRTEF LTLFISLVFT LLCNGVFWNA LLAGRDSLTS GTWLMLLCTG LLITGLQWLL LLLVATRWSV KPLLILLAVM TPAAVYFMRN YGVYLDKAML RNLMETDVRE ASELLQWRML PYLLVAAVSV WWIARVRVLR TGWKQAVMMR SACLAGALAM ISMGLWPVMD VLIPTLRENK PLRYLITPAN YVISGIRVLT EQASSSADEA REVVAADAHR GPQEQGRRPR ALVLVVGETV RAANWGLSGY ERQTTPELAA RDVINFSDVT SCGTDTATSL PCMFSLNGRR DYDERQIRRR ESVLHVLNRS DVNILWRDNQ SGCKGVCDGL PFENLSSAGH PTLCHGERCL DEILLEGLAE KITTSRSDML IVLHMLGNHG PAYFQRYPAS YRRWSPTCDT TDLASCSHEA LVNTYDNAVL YTDHVLARTI DLLSGIRSHD TALLYVSDHG ESLGEKGLYL HGIPYVIAPD EQIKVPMIWW QSSQVYADQA CMQTHASRAP VSHDHLFHTL LGMFDVKTAA YTPELDLLAT CRKGQPQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | chrB | ChrB | TnSen1.1 | 180 | 2994-3536 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Chromate | ||||||||||
| Protein Sequence: | MNAQNWLLLT YKVPPEPAKK RIALWRKLKG MGAVYLQNGV CLLPKTDDHT RRLKIIENEI NEMAGDSVLL ETVALDRAQE DKVIARFKAD RDEEYKELLG KCADFEAEIA HETEVQHFTY AELEENDVDL KKLLSWLEKI AKLDFYGATL AAEAAERLKG CEALLDAYAQ RVFDAHDENR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | TnSen1.1 | 186 | 3775-4335 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MHGQRIGYIR VSSFDQNPER QLEHVQVDKV FTDKASGKDT QRPELDRLLA FVREGDTVVV HSMDRLARNL DDLRRLVQGL TQRGVRIAFA KEHLTFTGED SPMANLMLSV MGAFAEFERA LIRERQREGI ALAKKRGAYR GRKKALSPDR VAELRQRAAA GEKKAHLARE FGISRETLYQ YLRTDD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | TnSen1.1 | 988 | 4338-7304 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPRRSILSVA ERASLLALPD TEDELIRLYT FSEADLSLIR QRRGDANRLG VGVQLCLLRF PGQGLLPDAT VPVSLLQWIG RQLRTDPACW PLYAERDETR REHLLELRAY LGMVPFGLAH YRQAVKATTE LALQTDKGIV LAASVLDALR HRHIILPTLD VVERVCAEAI TRANRRIYEA LSELLSSGHR HRLDDLLKRR DNGKTTWLAW LRQSPVKPNS RHMLEHIERL KAWQALDLPS GIERSVHQNR LLKIAREGGQ MTPADLAKFE PQRRYATLVA LAIEGMATVT DEIIDLHDRI LGKLFNAAKN KHQQQFQASG KAINAKVRLF GRIGQALIEA KQAGRDPFAA IEAVMSWDAF AESVTEAQKL AQPEDFDFLH RIGESYATLR RYAPEFLAVL KLRAAPAAKD VLDAIDVLRG MNSDNARKVP ADAPTGFIKP RWQKLVMADA SIDRRYYELC ALSELKNSLR SGDIWVQGSR QFKDFEDYLV PPAKFASLKL ASELPLAVAT DCDQYLNERL TLLETQLATV NRMAAANDLP DAIITESGLK ITPLDAAVPD TAQALIDQTA MILPHVKITE LLLEVDEWTG FTRHFTHLKS GDLAKDKNLL LTTILADAIN LGLTKMAESC PGTTYAKLAW LQAWHTRDET YSTALAELVN AQLRHPFAGH WGDGTTSSSD GQNFRTGSKA ESTGHINPKY GSSPGRTFYT HISDQYAPFH TKVVNVGVRD STYVLDGLLY HESDLRIEEH YTDTAGFTDH VFALMHLLGF RFAPRIRDLG DTKLYIPKGE AAYDALKPMI GGTLNIKHVR AHWEEILRLA TSIKQGTVTA SLMLRKLGSY PRQNGLAVAL RELGRIERTL FILDWLQSVE LRRRVHAGLN KGEARNALAR AVFFNRLGEI RDRSFEQQRY RASGLNLVTA AIVLWNTVYL ERAANALRGH GQAVDDGLLQ YLSPLGWEHI NLTGDYLWRS SAKIGAGKFR PLRPLSGS |
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1. Borowiak M, Fischer J, Hammerl JA, Hendriksen RS, Szabo I, Malorny B.
Identification of a novel transposon-associated phosphoethanolamine transferase gene, mcr-5, conferring colistin resistance in d-tartrate fermenting Salmonella enterica subsp. enterica serovar Paratyphi B.. J Antimicrob Chemother. 2017 Dec 1;72(12):3317-3324. doi: 10.1093/jac/dkx327. PubMed ID:
28962028
.