Mobile element type:
Transposon
Name:
TnSau9
Synonyms:
Tn7228
Accession:
TnSau9-AM408573.1
Family:
Compound Transposon
Group:
ISSau9
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Staphylococcus warneri
Date of Isolation:
2006
Country:
Denmark
Molecular Source:
plasmid pSCFS6
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| istA | ISSau9.1 | 319-1656 | + | Transposase | |
| cfrA (ARO:3003441) | TnSau9 | 2944-3993 | + | Passenger Gene | Antibiotic Resistance |
| lsaB (ARO:3003111) | TnSau9 | 5500-6978 | + | Passenger Gene | Antibiotic Resistance |
| istA | ISSau9 | 7461-8798 | + | Transposase | |
| istB | ISSau9 | 8773-9543 | + | Accessory Gene | ATPase Transposition Helper |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | istA | IstA | ISSau9.1 | 445 | 319-1656 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | Has two amino acid changes relative to istA (ISSau9) | ||||||||||
| Protein Sequence: | MTEEIVERLK GFIQDNEWKR KNNMSKQQMK KIDMYEKLQE ERYDIGYTTV RNFVNSEERR TKEVYIRQRY SAAWEVEFDW GEVKLVIDGK QKSYSLAVFT LAYSNYRFAL LYESETMICI QDAHTKLIDH LGFVPSVFTY DNMRTVVKSF VGTDRKITDG MINLSNYYEF RIRLCEPRKG NQKGHVERSV EVVRRKAFSY DIAFPSLEDA QKRLAQTILS LNKRIHHEKK LAHIELKDQE KVAAAQGALP LPFDVSEVVE CRVDKYSTVT IKQNRYSVPE GHVGAWIRAK VSADSVRLFI EGEFVAEHRR NWGVHQWEMD LYHYIKTFTK KKGALAQSEC LSQAPNQIKK LFENRYIGKE KDFLELLLYV REKNQWDNVM AAVRQIETKG LGDITTEKLI FLSEQTDLVP AVPLHPDETV EQALQNIKAF SAMFNQVDEG VLEHG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | cfrA (ARO:3003441) | CfrA | TnSau9 | 349 | 2944-3993 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | Cfr 23S ribosomal RNA methyltransferase (ARO:3000202) | ||||||||||
| Target: | oxazolidinone antibiotic (ARO:3000079)||streptogramin antibiotic (ARO:0000026)||pleuromutilin antibiotic (ARO:3000670)||phenicol antibiotic (ARO:3000387)||lincosamide antibiotic (ARO:0000017)||streptogramin A antibiotic (ARO:0000034) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3003441 | ||||||||||
| Protein Sequence: | MNFNNKTKYG KIQEFLRSNN EPDYRIKQIT NAIFKQRISR FEDMKVLPKL LREDLINNFG ETVLNIKLLA EQNSEQVTKV LFEVSKNERV ETVNMKYKAG WESFCISSQC GCNFGCKFCA TGDIGLKKNL TVDEITDQVL YFHLLGHQID SISFMGMGEA LANRQVFDAL DSFTDPNLFA LSPRRLSIST IGIIPSIKKI TQEYPQVNLT FSLHSPYSEE RSKLMPINDR YPIDEVMNIL DEHIRLTSRK VYIAYIMLPG VNDSLEHANE VVSLLKSRYK SGKLYHVNLI RYNPTISAPE MYGEANEGQV EAFYKVLKSA GIHVTIRSQF GIDIDAACGQ LYGNYQNSQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | lsaB (ARO:3003111) | LsaB | TnSau9 | 492 | 5500-6978 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target protection (ARO:0001003) | ||||||||||
| Sequence Family: | lsa-typeABC-F protein (ARO:3004472) | ||||||||||
| Target: | lincosamide antibiotic (ARO:0000017)||pleuromutilin antibiotic (ARO:3000670)||streptogramin antibiotic (ARO:0000026) | ||||||||||
| Comment: | ATPase component of ABC transporters with duplicated ATPase domains||strict match to reference sequence for ARO:3003111 (bitscore: 1006) | ||||||||||
| Protein Sequence: | MSMIHVQNLT FSYPSSFDNI FEDVSFQIDT DWKLGFIGRN GRGKTTLFNL LLDKFEYRGK IISSVDFNYF PYPVEDKSKY THEILEEICP QAEDWEFLRE IAYLNVDAEA MYRPFETLSN GEQTKVLLVA LFLNEGQFLL IDEPTNHLDT EARKTVSNYL RKKKGFILIS HDRNFLDGCV DHILSINRAD IEVQSGNYSS WKLNFDRQQG HEQATNERLQ KDIGRLEQST KRSAGWSNRV EASKNGTTNS GSKLDKGFVG HKAAKMMKRS KNLEARQQKS IEEKSKLLKN IEKTESLQFE PVEYKSKELI QLTDVSVIYD GQVVNKPISF NVEQGDRIVL DGKNGSGKSS ILKLILGDPI QYTGTLNTGS NLITSYVQQD TSHLKGMLAD FIEENEIDES LFKAILRKLD FDRVQFEKDI SHYSGGQKKK LLIAKSLCEK AHLYIWDEPL NFIDIYSRMQ IEELIQTFNP TMVFVEHDQT FQETISTKII KI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | istA | IstA | ISSau9 | 445 | 7461-8798 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MTEEIVERLK GFIQDNEWKR KNNMSKQQMK KIDMYEKLQE EGYDIGYTTV RNFVNSEERR TKEVYIRQRY SAAWEVEFDW GEVKLVIDGK QKSYSLAVFT LAYSNYRFAL LYESETMICI QDAHTKLIDH LGFVPSVFTY DNMRTVVKSF VGTDRKITDG MINLSNYYEF RIRLCEPRKG NQKGHVERSV EVVRRKAFSY DIAFPSLEDA QKRLAQTILS LNKRIHHEKK LAHIELKDQE KVAAAQEALP LPFDVSEVVE CRVDKYSTVT IKQNRYSVPE GHVGAWIRAK VSADSVRLFI EGEFVAEHRR NWGVHQWEMD LYHYIKTFTK KKGALAQSEC LSQAPNQIKK LFENHYIGKE KDFLELLLYV REKNQWDNVM AAVRQIETKG LGDITTEKLI FLSEQTDLVP AVPLHPDETV EQALQNIKAF SAMFNQVDEG VLEHG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | istB | IstB | ISSau9 | 256 | 8773-9543 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | ATPase Transposition Helper | ||||||||||
| Protein Sequence: | MKECLNMDKK QSVIDLCKEL RLPGIRELVE EDVAFQETTP AIDLLHTVLT KEKNDRWIRS KSTRIRRANF PEKKLLEEID TRALPTDAQQ KLPNLQTLDF IKAGQNVILT GSPGTGKSHL AIGLGVEACL AGYKVYFASV SSLINQLKES RSERTLRSFE LRFEKYDLVI IDELGYISFD KEGAELLFTH LSLRAGRSST IITSNLSFAK WEEVFHDPIL TAALTDRLTH KSHVVNMIGP SYRMRETQKW LENSHS |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| ISSau9.1-AM408573 | ISSau9.1 | insertion sequence | 1-2452 | + | 2452 |
| ISSau9-AM086211 | ISSau9 | insertion sequence | 7143-9594 | + | 2452 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRL | ISSau9 | 7143-7169 | + | 27 | TGTATATGTT AACTTAAAAC TGAGCCA |
| IRR | ISSau9 | 9568-9594 | - | 27 | TGTATTTCGC AACTGAAAAT TGAGCCA |
1. Kehrenberg C, Aarestrup FM, Schwarz S.
IS21-558 insertion sequences are involved in the mobility of the multiresistance gene cfr.. Antimicrob Agents Chemother. 2007 Feb;51(2):483-7. doi: 10.1128/AAC.01340-06. Epub 2006 Dec 4. PubMed ID:
17145796
.