Mobile element type:
Transposon
Name:
TnPa40.1
Synonyms:
Tn7174
Accession:
TnPa40.1-CP020704.1
Family:
Tn3
Group:
Tn21
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Pseudomonas aeruginosa PASGNDM699
Date of Isolation:
2017
Country:
Singapore
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-37 | + | 37 | GGGGTGCCCG CAGAATTCGG AAAAAATCGT ACGCTAA |
| IR int | 6500-6538 | + | 39 | GGGGAGCCTA GAGAATTCGG AAAAAATCGT ACGCTAAGG |
| IRR | 11718-11754 | - | 37 | GGGGAGCCCG CAGAACTCGG AAAAAATCGT ACGCTAA |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | TnPa40.1 | 7994-8116 | + | 123 | CGCTGTCAGG TTGGGCTATA CCCTAACTGG ATGTCAGGAA TGGCCACGCT GCGCCGTCAG AATAGAGTCT GCTTTCACAT TCTTTGACAC ATGCTTGCCA AGGTCATAGA TTCCAACCTG ACA |
| res_site_I | TnPa40.1 | 7994-8031 | + | 38 | CGCTGTCAGG TTGGGCTATA CCCTAACTGG ATGTCAGG |
| res_site_II | TnPa40.1 | 8056-8082 | + | 27 | TAGAGTCTGC TTTCACATTC TTTGACA |
| res_site_III | TnPa40.1 | 8085-8116 | + | 32 | TGCTTGCCAA GGTCATAGAT TCCAACCTGA CA |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnp | IS1411 | 625-1926 | - | Transposase | |
| amidase | TnPa40.1 | 2063-3562 | + | Passenger Gene | Other |
| WP_049251069.1 | TnPa40.1 | 3663-4010 | + | Passenger Gene | Hypothetical |
| tonB-dependent receptor | TnPa40.1 | 3878-5479 | + | Passenger Gene | Other |
| DNA-binding response regulator | TnPa40.1 | 5593-6210 | + | Passenger Gene | Other |
| WP_085286981.1 | TnPa40.1 | 7168-7905 | - | Passenger Gene | Hypothetical |
| tnpR | TnPa40.1 | 8137-8751 | + | Accessory Gene | Resolvase |
| tnpA | TnPa40.1 | 8755-11721 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | IS1411 | 433 | 625-1926 | - |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | VNNLTFSSPD LSSLCQLNNL GLTATGQHLC AERAVIECRL TKAPEPCPKC GAAGVSRGTV DRHLAHTPYG QRPTRLLLRI RRWRCACGCF WHEDTNSAAP PRSKLSYGAI RWALAAIVLD HLSVSRVASQ LDVAWHTANN AIINEGRRLL FNDSTRFDGV TVLGVDEHVW RHTRCGDKYV TIVVDLTPVR NKNGPARLLD VLEGRSKQAF KQWLQSRPKS WRDQIESIAM DGFTGFKSAA QEALPQAQTV LDPFHVVRWA SNMLDECRRR VQHDILGRRG RKNDPLYKSR RTLLTRISYL SDANKKQLFQ LFADEHHLEV DCTWSMYQRV VSAYNEPDRK RGKKLMEEVI NIITASDLPK ALIEVKGLGE TLKKYAESIL AYFDRPGTSN GPTEAINGRL EHLRGTALGF RNLTNYIARC LLKSGGFRNQ LHP |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | amidase | Amidase | TnPa40.1 | 499 | 2063-3562 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Protein Sequence: | MSVQQYLQAD ATALADLVQR GEVSAAELLA LATAQLQRLN PQLNAVNRPM PEQARQRVGE TLEGPLAGVP VLIKDAVQDY AGLPTSNGSR AFRHHVAASH STVVQRLLDA GAVIIGKTNT PELALKGFTD PQAFGITRNP WDLTRTPGGS SGGSAAAVAA GIVPMAGAND GGGSIRIPAA CCGLLGLRPS RGRVPCGPAS GEIWEGASSD LVVSRSVRDS ALALDVLAGP ACGEPFVIAP PAQSFRALLS EEPRRLRIAF STRSPLDTEV HPEAIAAVQH TVRLLEKLGH EVIEAEPRYD GHALARCYLD MYFGQVAATL AEAREAGARS ADFELATRLL EAFGNATRAG NYVRSHRRWN QFARALGEFH QRHDLFLTPT LACPPAPHAA AQMPAGQERL LGWLLNSGLL GVLARLGLME KPLNELSRQN LSYVPFTQLA NLTGTPAISV PLHWTAEGLP LGVQFIAPFG QEGRLLQLAA QLERAQPWMH RLPAMAYPT |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | WP_049251069.1 | WP_049251069.1 | TnPa40.1 | 115 | 3663-4010 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Protein Sequence: | MPRYPTLAAL IALLAFGSFT LFTLVHAEQS LLQFGRELLA RPDTAQVVID LYLMAGLGGI WMYRDARAKG RSWRSLLPYW LLTAVFVSLG PLLYLVVEGF RASCGHGAGR RQGLS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tonB-dependent receptor | TonB-dependent receptor | TnPa40.1 | 533 | 3878-5479 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Protein Sequence: | MALAATVLAA DGRFCLARPA ALSGGRGISR VLRPWRWPPP GSILGAWRQR LAAPITRSPF RLLRTLIVAM EQWYESTPGW YITPKTPEKR CKTLFAWLAL LIPDRLHAVK KTMSRSFSMQ YSRLPHAIAL TLAGFSSYSL ANGLALNEQS VSGMGTAFAG RASSALDAST LYGNPAGMSK LKRTEVVGGA AYVKPKTDIS DVNTSVSGTS KGDIAPGAAV PFGYVVTPLN EDFHFGLGFY VPFGVVSDNE KSFQGRAHGL YSKVQVTTLQ PTISYRISDT LSVGFGPTLN KIDGKLTNRL DTSMLGGSGG TSLKIKGDDI AYGFNLGVLW DVTDQLALGL TYHSKVEYEL EGHTKVANSP AGMLDGRFDT SLDITTPESI DASLTYRLNE RWTTYAGITW TRWSRLDKIV AETSSAFLGD IDEQMGWHDT WSYAIGAAYQ LNPQWVLRAG LALDQSPTSN AERNVRIPVG DRKIFSLGAG WSPTPDVTID LAYSYLREES VNVNRAADTF KPAYSAQYQN SAHGIGIQLT YRF |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | DNA-binding response regulator | DNA-binding response regulator | TnPa40.1 | 205 | 5593-6210 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Protein Sequence: | MTALCTSLPE RLSLLLVDDH KIFLDGLTLA LRPLTRELQI HTAQNAVEAE AWLQKCDFDL VLLDLRLPDV PGLELLQRWQ KQRRMTPVAI LSASDSSQDA RAALAAGALG FIAKSADGEA IRQAVTRVLL GETLPAPAPQ EICRLTPRQQ EILPLLAEGL PNKLISRRLG LSEDTVKTHL KTLYQELNVH TRTACVSAAR QRGWL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | WP_085286981.1 | WP_085286981.1 | TnPa40.1 | 245 | 7168-7905 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Protein Sequence: | MNHSSPLTLQ DATHRDSAWE RAANYEKEER PIQPVRKIVV KGAADVVFFR SSSAHLVVAG ENQEAIRSIK TRLEGDELVI EQEGISISGG GANIHISGTG NIFVGGTINV GGRQGGVTMQ FKGRCIVGVA LPEAPSIRIK GSGDVTLYDL QQAVLDLGVQ GSGDITAFGR VDLLDAEVTG SGDVDTSELV ATSAKLSVAG SGDIDAYVSN SVKARVAGSG DIVIRGNPPI RDHSVAGSGD IKFKK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | TnPa40.1 | 204 | 8137-8751 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MEPKTNVSPT AHPAYGGILQ GQRIGYVRVS SYDQNPERQL EQVEIGKLFT DKASGKDTQR PQLEAMLGFV REGDTVVVHS MDRLARNLDD LRRLVQRLTK RGVRIEFLKE GLVFTGEDSP MANLMLSVMG AFAEFERALI RERQREGIAL AKQRGAYRGR KKALSDDQAI TLRQRAAAGE PKAQLAREFG ISRETLYQYL RTDD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | TnPa40.1 | 988 | 8755-11721 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPRRSILSAT ERDTLLALPE SQDDLIRYYT FNDSDLSLIR QRRGDGNRLG FAVQLCLLRY PGYALGNDTA LPEPVIQWVA KQVQADPASW AKYGERDVTR REHALELRTY LQLAPFGLSD FRALVRELTE LAQQTDKGLL LAGQALESLR QKRRILPTLS VIDRACSEAI ARANRRVYRA LIEPLNPSHH AKLDELLTIK AGSNSTWLTW LRQSPLKPNS RHMIEHIERL KIFQLVGLPD SLGRHIHQNR LLKLAREGGQ MTPQDLGKFE PERRYATLVA VVLESTATVI DELVDLHDRI LVKLFSGAKH KHQQQFQKQG KAINDKVRLY SKIGQALLDA KESGDDPFAA IEAVIPWDEF TQSVTEAELL ARPEAFDHLH LVSENFATLR RYTPAFLEVL QLRAATAAQA VLDAVQTLRE MNADNLRKVP SDAPTAFIKP RWKPLVITPE GIDRRFYEIC ALSELKNALR SGDIWVKGSR QFRDFDDYLL PPEKFAALKR EQALPLAINP NSDQYLEERL QLLDEQLATV TRLAKDNELP DAILTESGLK ITPLDSAVPN TAQALIDQTS QLLPRIKITE LLMDVDEWTG FTRHFTHLKD GAQAKDRTLL LTAILGDAIN LGLTKMAESS PGMTYAKLSW LQAWHIRDET YSTALAELVN SQFRHAFAAN WGDGTTSSSD GQRFRAGGKG ESTGHVNPKY GSEPGRLFYT HISDQYAPFS TRVVNVGVRD STYVLDGLLY HESDLRIEEH YTDTAGFTDH VFALMHLLGF RFAPRIRDLG ETKLYVPNSV QDYPTLRPML GGTLNIKHVC AHWDEILRLA ASIKQGTVTA SLMLRKLGSY PRQNGLAVAL RELGRIERTL FILDWLQSVE LRRRVHAGLN KGEARNSLAR AVFFNRLGEI RDRSFEQQRY RASGLNLVTA AIVLWNTVYL ERATQAREEA GKPVNPELLQ YLSPLGWEHI NLTGDYVWRQ SRKLEDGKFR PLRQLGKP |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| IS1411-M57500 | IS1411 | insertion sequence | 613-2031 | + | 1419 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRR | IS1411 | 613-636 | + | 24 | GGCTCTTCGC ATTTAAGGGT GTAG |
| IRL | IS1411 | 2008-2031 | - | 24 | GGCTCTTCGC ATTTAAGGGT GTAG |