Mobile element type:
Transposon
Name:
TnOtChr.1
Synonyms:
Tn7170
Accession:
TnOtChr.1-FN821089.1
Family:
Tn3
Group:
Tn21
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Pseudomonas sp. Tik3
Date of Isolation:
2011
Country:
Russia
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRR | 7143-7180 | - | 38 | GGGGTCGTCT CAGAAAACGG ACCGCAAAGT ACGCTAAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpR | TnOtChr.1 | 44-610 | - | Accessory Gene | Resolvase |
| chrB | TnOtChr.1 | 781-1719 | + | Passenger Gene | Heavy Metal Resistance |
| chrA | TnOtChr.1 | 1716-3083 | + | Passenger Gene | Heavy Metal Resistance |
| chrC | TnOtChr.1 | 3093-3701 | + | Passenger Gene | Heavy Metal Resistance |
| chrF | TnOtChr.1 | 3698-4150 | + | Passenger Gene | Heavy Metal Resistance |
| tnpA | TnOtChr.1 | 4181-7147 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | TnOtChr.1 | 188 | 44-610 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MRIGYARVST QEQDNQAQIS ALQSAGCELI FQEKASGGRW DRPELHRLLG HLRKADVVVV WKLDRLSRSL KDLLLTLEKI EEAGAGFQSL TESIDTTTPA GRMMMQIVGS FAEFERAMLR ERTRHGLEAA RKDGRVGGRR PKLTQQQQKE IVALITSGQK TGADAARLFR VHPSTVVRLL AKHRQGPG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | chrB | ChrB | TnOtChr.1 | 312 | 781-1719 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Chromate | ||||||||||
| Comment: | chromate-sensitive regulator of chrBACF operon exclusively activated by Cr(VI) | ||||||||||
| Protein Sequence: | MNLLSLILSL PTENATVRQR TWRALKASGA AVLRDGVYLM PDRDECRAVL DNLASDVREG GGVAHVLRME DPEGVNFVAL FDRSNDFAAL LVDVHHLRQT LTLDTVQDVL RQVRKLRKSF TTLVEIDFYP GEAQRQADSA LCELEQACAR TLSPDEPHAV EGTITRLDRL DYQARTWATR ARPWVDRLAS AWLIRRFIDP QARILWLATP ADCPPDALGF DFDGATFSHV GSRVTFEVLA ASFGLEQPAI TRIGLVVHYL DVGGIQPPEA TGIESVLAGL RETVDHDDQL LAIASTVFDG LLASFEKGTL TV |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | chrA | ChrA | TnOtChr.1 | 455 | 1716-3083 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Chromate | ||||||||||
| Comment: | chromate efflux pump | ||||||||||
| Protein Sequence: | MTQTVVTKEA VAVPPSPQVV SFWQAFRLWL KIGFIGFGGP AGQIAIMHRE LVEQRRWISE RRFLHALNFC MLLPGPEGQQ LATYMGWLMH RTWGGIVAGV LFVLPSLFFL IALSWIYIAF GDTPLVSGLF YGIKPAITAV VVQAVHRIGS RALKNGLMWA IAAGAFVAIF AMNVPFPAIV AGAALIGYIG GRVTPDKFRA GGAHRAADKS YGPALIDDNT PIPSHALFRW SGSLRVAAMG CLLWLIPMAF LLGTYGWDHT LTQMGWFFTK AALLTFGGAY AVLPYIYQGA VGHYSWLTPT QMVDGLALGE ANPGPLIMVV AFVGFVGAYV QALFGPDMLF VAGAVAAALV TWFTFLPSFI FILAGGPFVE STRGDLRFTA PLTAITAAVV GVILNLAVFF AYHVLWPKGL AGLFDWVSAL ITIGAAVALF RFKRNVIHVI AACAVLGLIL KTLIL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | chrC | ChrC | TnOtChr.1 | 202 | 3093-3701 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Chromate | ||||||||||
| Comment: | superoxide dismutase unkown function | ||||||||||
| Protein Sequence: | MSFDIKPLPF PASTLNGLSE KLIASHHENN YSGAVKRLNA IHTKLGTLDF TQEAGFLLNG LKREELIAYN SMLLHEIYFD SLGGNGVLPV GPLCEAIERD FGSVERWQAE FSAMGKALGG GSGWVLLVQS ARDGKLSNQW AADHCHTLAG ALPILALDMY EHAYHIDYGA KAGAYVDAFM ANIDWQRAAI RWASHQPSGG LS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | chrF | ChrF | TnOtChr.1 | 150 | 3698-4150 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Chromate | ||||||||||
| Protein Sequence: | MKWITRERPK IDRIACPWMV TRFIDTHAEF LYVPAGDVLR IAQETGAVPY DIPGVELSHD GELCSFDAFL AKYAMDDPAL KQLAVIVRGA DTSRLDLTPQ STGLYALSLG LSQNFSDDHE MLKHGMVMYD AFYAWCKHCQ SETHNWPPKM |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | TnOtChr.1 | 988 | 4181-7147 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPRRSILSAA ERESLLALPD TMEDLIRYYT FSESDLSIIR QRRGPANLLG FAVQLCYMRY PGMMLAVDTE PFPPLLRLVA TQLKVPPEAW ADYGQRAETR REHLLELQSI FGFQAFATRH YRPSVHSLDE LAWQTDKGIV LATELIEGLR QKSVLLPSPG VIERICAEAI TRANRRIYEA LSEPLTDPHR HRLDDLLKRR EFGKTTWLAW LRQSPVKPNS RHMLEHIERL KAWQALDLPT GIERLLHQNR LLKIAREGGQ MTPADLAKFE PQRRYATLVA LAIEGMATVI DEIIDLHDRI LGKLFNAAKH KHQQQFQASG KAINAKVRLY GRIGQALIDA KQSGGNPFAA IEAVMSWDAF AQSVTEAQKL AQPDDFDFLH RIGESYATLR RYAPEFLAVL KLRAAPAAKG VLDAVDVLRG MNTDNVRKVP TDAPTDFIKP RWQKLVMTDA GIDRRYYELC ALSELKNSLR SGDIWVQGSR QFKDFEDYLV PHEKFASLKQ GSELPLDVAT DCDQYLYERL TLLEAQLATV NRMAAANDLP DAIITESGLK ITPLDAAVPD TAQALIDQTA MILPHVKITE LLLEVDEWTG FTRYFTHLKS GDLAKDKNLL LTTILADAIN LGLSKMAESC PGTTYAKLAW LQAWHIRDET YSTALAELVN TQFRHPFACH WGDGTTSSSD GQNFRTGSKA KSTGHINPKY GSSPGRTFYT HISDQYAPFH AKVVNVGVRD STYVLDGLLY HESDLRIEEH YTDTAGFTDH VFALMHLLGF RFAPRIRDLG DTKLYLPKGD TAYEALKPMV GGTLNIKHVR AHWDEILRLA TSIKHGTVTA SLMLRKLGSY PRQNGLAVAL RELGRIERTL FILDWLQSVE LRRRVHAGLN KGEARNALAR AVFFNRLGEI RDRSFEQQRY RASGLNLVTA AVVLWNTVYL ERAAHALRGN GLGIDDALLQ YLSPLGWEHI NLTGDYLWRS SAKIGAGKFR PLRPLQPP |
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1. Petrova M, Gorlenko Z, Mindlin S.
Tn5045, a novel integron-containing antibiotic and chromate resistance transposon isolated from a permafrost bacterium.. Res Microbiol. 2011 Apr;162(3):337-45. doi: 10.1016/j.resmic.2011.01.003. Epub 2011 Jan 22. PubMed ID:
21262357
.