Mobile element type:
Transposon
Name:
TnOtChr
Synonyms:
Tn7169
Accession:
TnOtChr-EF469735.1
Family:
Tn3
Group:
Tn21
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Ochrobactrum tritici5bvl1
Date of Isolation:
2008
Country:
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGTCGTCT CAGAAAACGG ACCGCAAAGT ACGCTAAG |
| IRR | 7152-7189 | - | 38 | GGGGTCGTCT CAGAAAACGG ACCGCAAAGT ACGCTAAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpR | TnOtChr | 44-610 | - | Accessory Gene | Resolvase |
| chrB | TnOtChr | 781-1719 | + | Passenger Gene | Heavy Metal Resistance |
| chrA | TnOtChr | 1716-3092 | + | Passenger Gene | Heavy Metal Resistance |
| chrC | TnOtChr | 3102-3710 | + | Passenger Gene | Heavy Metal Resistance |
| chrF | TnOtChr | 3707-4159 | + | Passenger Gene | Heavy Metal Resistance |
| tnpA | TnOtChr | 4190-7156 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | TnOtChr | 188 | 44-610 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MRIGYARVST QEQDNQAQIS ALQSAGCELI FQEKASGGRW DRPELHLLLG HLRKADVVVV WKLDRLSRSL KDLLLTLEKI EEAGAGFQSL TESIDTTTPA GRMMMQIVGS FAEFERAMLR ERTRHGLEAA RKDGRVGGRR PKLTQQQQKE IVALITSGQK TGADAARLFR VHPSTVVRLL AKHRQGPG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | chrB | ChrB | TnOtChr | 312 | 781-1719 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Chromate | ||||||||||
| Comment: | chromate-sensitive regulator of chrBACF operon exclusively activated by Cr(VI) | ||||||||||
| Protein Sequence: | MNLLSPILSL PTENATVRQR TWRALKASGA AVLRDGVYLM PDRDECRAVL DNLASDVREG GGVAHVLRME DPEGVNFVAL FDRSNDFAAL LVDVHHLRQT LTLDTVQDVL RQVRKLRKSF TTLVEIDFYP GEAQRQADSA LCELEQACAR TLSPDEPHAV EGTITRLDRL DYQARTWATR ARPWVDRLAS AWLIRRFIDP QARILWLATP ADCPPDALGF DFDGATFSHV GSRVTFEVLA ASFGLEQPAI TRIGLVVHYL DVGGIQPPEA TGIESVLAGL RETVDHDDQL LAIASTVFDG LLASFEKGTL TV |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | chrA | ChrA | TnOtChr | 458 | 1716-3092 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Chromate | ||||||||||
| Comment: | chromate efflux pump | ||||||||||
| Protein Sequence: | MTQTVVTKEA VAVPPSPQVV SFWQAFRLWL KIGFIGFGGP AGQIAIMHRE LVEQRRWISE RRFLHALNFC MLLPGPEGQQ LATYMGWLMH RTWGGIVAGV LFVLPSLFFL IALSWIYIAF GDTPLVSGLF YGIKPAITAV VVQAVHRIGS RALKNGLMGL MWAIAAGAFV AIFAMNVPFP AIVAGAALIG YIGGRVTPDK FRAGGAHRAA DKSYGPVLID DNTPIPSHAL FRWSGLLRVA AMGCLLWLIP MAFLLGTYGW DHTLTQMGWF FTKAALLTFG GAYAVLPYIY QGAVGHYSWL TPTQMVDGLA LGEANPGPLI MVVAFVGFVG AYVQALFGPD MLFVAGAVAA ALVTWFTFLP SFIFILAGGP FVESTRGDLR FTAPLTAITA AVVGVILNLA VFFAYHVLWP KGLAGHFDWV SALITIGAAV ALFRFKRNVI HVIAACAVLG LILKTLIL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | chrC | ChrC | TnOtChr | 202 | 3102-3710 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Chromate | ||||||||||
| Comment: | superoxide dismutase unkown function | ||||||||||
| Protein Sequence: | MSFDIKPLPF PASTLNGLSE KLIASHHENN YSGAVKRLNA IHTKLGTLDF TQEAGFLLNG LKREELIAYN SMLLHEIYFD SLGGNGVLPV GPLCEAIERD FGSVERWQAE FSAMGKALGG GSGWVLLVQS ARDGKLSNQW AADHCHTLAG ALPILALDMY EHAYHIDYGA KAGAYVDAFM ANIDWQRAAI RWASHQPSGG LS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | chrF | ChrF | TnOtChr | 150 | 3707-4159 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Chromate | ||||||||||
| Protein Sequence: | MKWITRERPK IDRIACPWLV TRFIDTDAEF LYVPAGDVLR IAQETGAVPY DIPGVELSHD GELCSFDAFL AKYAMDDPAL KQLAVIVRGA DTSRLDLTPQ SAGLYALSLG LSQNFSDDHE MLKHGMVMYD AFYAWCKHCQ SETHNWPPKM |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | TnOtChr | 988 | 4190-7156 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPRRSILSAA ERESLLALPD TMEDLIRYYT FSESDLSIIR QRRGPANLLG FAVQLCYMRY PGMMLAVDTE PFPPLLRLVA TQLKVPPEAW ADYGQRAETR REHLLELQSI FGFQAFATRH YHPSVHSLDE LAWQTDKGIV LATELIEGLR QKSVLLPSPG VIERICAEAI TRANRRIYEA LSEPLTDPHR HRLDDLLKRR EFGKTTWLAW LRQSPVKPNS RHMLEHIERL KAWQALDLPT GIERLLHQNR LLKIAREGGQ MTPADLAKFE PQRRYATLVA LAIEGMATVI DEIIDLHDRI LGKLFNAAKH KHQQQFQASG KAINAKVRLY GRIGQALIDA KQSGGNPFAA IEAVMSWDAF AQSVTEAQKL AQPDDFDFLH RIGESYATLR RYAPEFLAVL KLRAAPAAKG VLDAVDVLRG MNTDNVRKVP TDAPTDFIKP RWQKLVMTDA GIDRRYYELC ALSELKNSLR SGDIWVQGSR QFKDFEDYLV PHEKFASLKQ GSELPLDVAT DCDQYLYERL TLLEAQLATV NRMAAANDLP DAIITESGLK ITPLDAAVPD TAQALIDQTA MILPHVKITE LLLEVDEWTG FTRYFTHLKS GDLAKDKNLL LTTILADAIN LGLSKMAESC PGTTYAKLAW LQAWHIRDET YSTALAELVN TQFRHPFAGH WGDGTTSSSD GQNFRTGSKA KSTGHINPKY GSSPGRTFYT HISDQYAPFH AKVVNVGVRD STYVLDGLLY HESDLRIEEH YTDTAGFTDH VFALMHLLGY RFAPRIRDLG DTKLYLPKGD TAYEALKPMV GGTLNIKHVR AHWDEILRLA TSIKHGTVTA SLMLRKLGSY PRQNGLAVAL RELGRIERTL FILDRLQSVE LRRRDHAGLN KGEARNALAR AVFFNRLGEI RDRSFEQQRY RASGLNLVTA AVVLWNTVYL ERAAHALRGN GLGVDDALLQ YLSPLGWEHI NLTGDYLWRS SAKIGAGKFR PLRPLQPP |
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1. Branco R, Chung AP, Johnston T, Gurel V, Morais P, Zhitkovich A.
The chromate-inducible chrBACF operon from the transposable element TnOtChr confers resistance to chromium(VI) and superoxide.. J Bacteriol. 2008 Nov;190(21):6996-7003. doi: 10.1128/JB.00289-08. Epub 2008 Sep 5. PubMed ID:
18776016
.