Mobile element type:
Transposon
Name:
TnMCR5ECO26H11
Synonyms:
Tn7163
Accession:
TnMCR5ECO26H11-BEPM01000040
Family:
Tn3
Group:
Tn21
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Escherichia coliO26:H11 10875
Date of Isolation:
2017
Country:
Molecular Source:
scaffold40
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-39 | + | 39 | GGGGTCGTCT CAGAAAACGG AAAAAATCGT ACGCTAAGC |
| IRR | 7294-7334 | - | 41 | GGGGTCGTCT CAGAAAACGG AAAATAAAGC ACGCTAAGCC G |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res_site_II | TnMCR5ECO26H11 | 3683-3726 | + | 44 | ATTGCGTCAG GATAGGATTG AATTTTGAAT TTATTGACAT ATCT |
| res_site_III | TnMCR5ECO26H11 | 3730-3761 | + | 32 | TGAAGGTCAT AGAGTCTTCC CTGACATTTT GC |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| msf2 | TnMCR5ECO26H11 | 101-778 | - | Passenger Gene | Other |
| msf | TnMCR5ECO26H11 | 775-1335 | - | Passenger Gene | Other |
| MCR-5.1 (ARO:3004332) | TnMCR5ECO26H11 | 1332-2975 | - | Passenger Gene | Antibiotic Resistance |
| chrB | TnMCR5ECO26H11 | 2994-3536 | - | Passenger Gene | Heavy Metal Resistance |
| tnpR | TnMCR5ECO26H11 | 3772-4332 | + | Accessory Gene | Resolvase |
| tnpA | TnMCR5ECO26H11 | 4335-7301 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | msf2 | Msf2 | TnMCR5ECO26H11 | 225 | 101-778 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | permease | ||||||||||
| Protein Sequence: | VIPGVLAVLA FLTLVKDPEH SPNPALKFFS SLRGLPARFK RYLGAVGIFG IGDFSHSLLI LAATTLLTPS LGVIQAAQVA GLLYVWRNLV QVAVSYPVGV LADRVGHLPV LIAGYVLGTL TAVLTALAFW LGIASVPLLA GVFFIAGLYV AVQEALESTV TAEMVQPDTL AMSYGALGTV NGAAKFISSA SVGVVWTAVS PVVGFGLAAV LMAAGTLTLL RVKGN |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | msf | Msf | TnMCR5ECO26H11 | 186 | 775-1335 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | permease | ||||||||||
| Protein Sequence: | MTTSTDDPPK RWLNRTVAGA GITSSLGDFC YETTTVILPG FLAVLGIPAA VLGLIEGIAD AIAAFTKMVS GYIADKLGHR KLFVMVGYGL TPLGQVLIAL AAGWPLLLLG RIVSWFGKGL RGPLRDAIVI QAVSPETRGR AFGFHRAADT VGAVSAPCWA SRCWVGRKAF IGATRLDPSG SYYGCR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | MCR-5.1 (ARO:3004332) | MCR-5.1 | TnMCR5ECO26H11 | 547 | 1332-2975 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | MCR phosphoethanolamine transferase (ARO:3004268) | ||||||||||
| Target: | peptide antibiotic (ARO:3000053) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3004332 (bitscore: 1127)||Synonyms: | ||||||||||
| Protein Sequence: | MRLSAFITFL KMRPQVRTEF LTLFISLVFT LLCNGVFWNA LLAGRDSLTS GTWLMLLCTG LLITGLQWLL LLLVATRWSV KPLLILLAVM TPAAVYFMRN YGVYLDKAML RNLMETDVRE ASELLQWRML PYLLVAAVSV WWIARVRVLR TGWKQAVMMR SACLAGALAM ISMGLWPVMD VLIPTLRENK PLRYLITPAN YVISGIRVLT EQASSSADEA REVVAADAHR GPQEQGRRPR ALVLVVGETV RAANWGLSGY ERQTTPELAA RDVINFSDVT SCGTDTATSL PCMFSLNGRR DYDERQIRRR ESVLHVLNRS DVNILWRDNQ SGCKGVCDGL PFENLSSAGH PTLCHGERCL DEILLEGLAE KITTSRSDML IVLHMLGNHG PAYFQRYPAS YRRWSPTCDT TDLASCSHEA LVNTYDNAVL YTDHVLARTI DLLSGIRSHD TALLYVSDHG ESLGEKGLYL HGIPYVIAPD EQIKVPMIWW QSSQVYADQA CMQTHASRAP VSHDHLFHTL LGMFDVKTAA YTPELDLLAT CRKGQPQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | chrB | ChrB | TnMCR5ECO26H11 | 180 | 2994-3536 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Chromate | ||||||||||
| Protein Sequence: | MNAQNWLLLT YKVPPEPAKK RIALWRKLKG MGAVYLQNGV CLLPKTDDHT RRLKIIENEI NEMAGDSVLL ETVALDRAQE DKVIARFKAD RDEEYKELLG KCADFEAEIA HETEVQHFTY AELEENDVDL KKLLSWLEKI AKLDFYGATL AAEAAERLKG CEALLDAYAQ RVLDAHDENR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | TnMCR5ECO26H11 | 186 | 3772-4332 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | resolvase||serine site-specific recombinase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | ) | ||||||||||
| Protein Sequence: | MTGQRIGYIR VSTFDQNPER QLEGVKVDRA FSDKASGKDV KRPQLEALIS FARTGDTVVV HSMDRLARNL DDLRRIVQTL TQRGVHIEFV KEHLSFTGED SPMANLMLSV MGAFAEFERA LIRERQREGI ALAKQRGAYR GRKKSLSSER IAELRQRVEA GEQKTKLARE FGISRETLYQ YLRTDQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | TnMCR5ECO26H11 | 988 | 4335-7301 | + |
|---|
| Class: | Transposase | ||||||||||
| Function: | transposition, DNA-mediated (GO:0006313) | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | identical to TnAs3 tnpA | ||||||||||
| Protein Sequence: | MPRRSILSAA ERESLLALPD SKDDLIRHYT FNDTDLSIIR QRRGPANRLG FAVQLCYLRF PGVILGVDEL PFPPLLKLVA DQLKVGVESW NEYGQREQTR REHLSELQTV FGFRPFTMSH YRQAVQMLTE LAMQTDKGIV LASALIGHLR RQSVILPALN AVERASAEAI TRANRRIYDA LAEPLADAHR RRLDDLLKRR DNGKTTWLAW LRQSPAKPNS RHMLEHIERL KAWQALDLPT GIERLVHQNR LLKIAREGGQ MTPADLAKFE PQRRYATLVA LATEGMATVT DEIIDLHDRI LGKLFNAAKN KHQQQFQASG KAINAKVRLY GRIGQALIDA KQSGRDAFAA IEAVMSWDSF AESVTEAQKL AQPDDFDFLH RIGESYATLR RYAPEFLAVL KLRAAPAAKN VLDAIEVLRG MNTDNARKLP ADAPTGFIKP RWQKLVMTDA GIDRRYYELC ALSELKNSLR SGDIWVQGSR QFKDFEDYLV PPEKFTSLKQ SSELPLAVAT DCEQYLHERL TLLEAQLATV NRMAAANDLP DAIITESGLK ITPLDAAVPD TAQALIDQTA MVLPHVKITE LLLEVDEWTG FTRHFTHLKS GDLAKDKNLL LTTILADAIN LGLTKMAESC PGTTYAKLAW LQAWHTRDET YSTALAELVN AQFRHPFAGH WGDGTTSSSD GQNFRTASKA KSTGHINPKY GSSPGRTFYT HISDQYAPFH TKVVNVGLRD STYVLDGLLY HESDLRIEEH YTDTAGFTDH VFALMHLLGF RFAPRIRDLG DTKLYIPKGD AAYDALKPMI GGTLNIKHVR AHWDEILRLA TSIKQGTVTA SLMLRKLGSY PRQNGLAVAL RELGRIERTL FILDWLQSVE LRRRVHAGLN KGEARNALAR AVFFNRLGEI RDRSFEQQRY RASGLNLVTA AIVLWNTVYL ERAAHALRGN GHAVDDSLLQ YLSPLGWEHI NLTGDYLWRS SAKIGAGKFR PLRPLQPA |
||||||||||