Mobile element type:
Transposon
Name:
TnLfArs
Synonyms:
Tn7162
Accession:
TnLfArs-DQ057986.1
Family:
Tn3
Group:
Tn21
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Leptospirillum ferriphilum Fairview
Date of Isolation:
2006
Country:
South africa
Molecular Source:
plasmid pLfTnArs
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 5-43 | + | 39 | GGGGTCGTCT CAGAAAACGG AAAACAAAGC ACGTTAAGC |
| IRR | 8728-8768 | - | 41 | GGGGTCGTCT CAGAAAACGG AGAATAAAGC ACGCTAAGCC G |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpR | TnLfArs | 38-601 | - | Accessory Gene | Resolvase |
| arsR | TnLfArs | 787-1164 | + | Passenger Gene | Heavy Metal Resistance |
| arsC | TnLfArs | 1161-1640 | + | Passenger Gene | Heavy Metal Resistance |
| arsD | TnLfArs | 1681-2049 | + | Passenger Gene | Heavy Metal Resistance |
| arsA | TnLfArs | 2055-3830 | + | Passenger Gene | Heavy Metal Resistance |
| CBS domain-like protein | TnLfArs | 3841-4314 | + | Passenger Gene | Heavy Metal Resistance |
| arsB | TnLfArs | 4316-5602 | + | Passenger Gene | Heavy Metal Resistance |
| tnpA | TnLfArs | 5769-8735 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | TnLfArs | 187 | 38-601 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MQGQRIGYVR VSSFDQNPER QLEQAEVGKV FTDKASGKDT QRPELDSLLA FVREGDTVVV HSMDRLARNL DDLRRLVQKL TKRGVRIEFV KECLTFTGED SPMANLMLSV MGAFAEFERA LIRERQKEGI ALAKQRGAYR GRKKALSPER AAELLQRVNA GEQKAKLARE FGISRETLYQ YLREIRA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | arsR | ArsR | TnLfArs | 125 | 787-1164 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Arsenic | ||||||||||
| Comment: | arsenic operon regulator | ||||||||||
| Protein Sequence: | MEKQAATSIF ESLSSGLRLD VFRLLVKKGP GGMVAGEIAN ALDIPPANLS FHLKALSQAH LVTVEQEGRF QRYRADIPLM LDLIAYLTEE CCSGSPDQCL ELRTASKCSE EFLPLLSPTP TKATT |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | arsC | ArsC | TnLfArs | 159 | 1161-1640 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Arsenic | ||||||||||
| Comment: | cytoplasmic arsenate reductase | ||||||||||
| Protein Sequence: | MNILFLCTGN SCRSILAEAT FNHLAPTGWK AMSAGSQPTG TVHPRSLSLL THEGIDTGGL HSKSWDNLPL APDVVITVCA NAAGETCPAY LGPVLRAHWG VDDPAHATGT EAEIEAAFPT AYRILRRRIE ALLALPLAQL AHDRTRLKAE LDRIGALSA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | arsD | ArsD | TnLfArs | 122 | 1681-2049 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Arsenic | ||||||||||
| Comment: | sarsenic operon regulator | ||||||||||
| Protein Sequence: | MKKIEVFDPS LCCSTGVCGV DVDQALVTFA ADVDWAKQNG AHIERYNLAQ QPQMFADNAT VKGFLQRSGQ DALPLILVDG EVALAGRYPK RAELALWIGI DQPADAAKPT TGGCCSGPRG CC |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | arsA | ArsA | TnLfArs | 591 | 2055-3830 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Arsenic | ||||||||||
| Comment: | ATPase inner membrane efflux pump for extrusion of arsenite, antimonite and arsenate | ||||||||||
| Protein Sequence: | MEKMMMIFLQ LPPRFLFFTG KGGVGKTSIA CATAIQLAEA GKRVLLVSTD PASNVGQVFG VDIGNRVTPI PAVPRLSALE IDPEAAASAY RERLVGPVRG VLPDDVVKGI EESLSGACTT EIAAFDEFTA LLTNVALTAD YEHIIFDTAP TGHTIRLLQL PGAWSGFLEA GKGDASCLGP LAGLEKQRTQ YKAAVEALAD PLQTRLVLVA RAQQATLREV ARTHEELAAI GLKQQHLVIN GILPHIEAAT DPLAAAIHER EQTALKNIPA TLTALPCDHV ELKPFNLVGL DALRQLLTDL PPQAPVAVDS PIELDEPGVA DLIDGIAADG HGLVMLMGKG GVGKTTLAAA IAVELAHRGL PVHLTTSDPA AHLTDTLDSS LDNLTVSRID PHAETERYRQ HVLETKGAQL DAEGRALLEE DLRSPCTEEI AVFQAFSRII REAGKKFVVM DTAPTGHTLL LLDATGAYHR EVTRQMGKTG MHFTTPMMQL QDPKQTKVLI VTLAETTPVL EAANLQADLR RAGIEPWAWI INTSVAAASA KSPLLRQRAA NELREISAVA NQHADRYAVV PLLKEEPIGA DRLRALIHPQ T |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | CBS domain-like protein | CBS domain-like protein | TnLfArs | 157 | 3841-4314 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Arsenic | ||||||||||
| Comment: | possible arsenical efflux pump membrane protein sensor | ||||||||||
| Protein Sequence: | MLIRNFMTPD PVTIQPETPV EDIARLLLAH RINGVPVVDG AGRLIGVVTA EDLIHRGADE RLEPRESIWK ENFWVSFLGP KGTQRDKAEG RTAAEVMTTE VHSVTPAMHP SVAARLMVDH HLTALPVVDD GKVIGVISRI DLLSLLKELE NPLKREN |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | arsB | ArsB | TnLfArs | 428 | 4316-5602 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Arsenic | ||||||||||
| Comment: | Na+/H+ antiporter NhaD or related arsenite permease, arsenical pump membrane protein | ||||||||||
| Protein Sequence: | MIAALPIFLA TIVLVIWQPR GLGIGWSATL GAVVALLSGV VHFGDIPVVW QIVWNATATF IAIIIISLLL DEAGFFEWAA LHVARWGGGK GRLLFALIIL LGAAVAALFA NDGAALILTP IVMAMLLALG FSPAATLAFV MAAGFIADAA SLPLVVSNLV NIVSADFFNI GFNDYAAVMI PVNIVAIIAS LAVLSIYFRR SIPAHYDVNQ LKQPNEAIRD VATFRFGWVV LALLLVGFFG LEPLGVPVSA VAAAGALLLL AVAARGHVIS TRKVIREAPW QIVVFSLGMY LVVYGLRNQG LAEHIARLLD YFAQGGVWGA AFGTGFLTAL LSSAMNNMPT VLVGALSIDA TSATGVVKDT MIYANVIGSD LGPKITPIGS LATLLWLHVL ARKGMTITWG YYFKVGVVLT VPVLAATLAA LALRLSLT |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | TnLfArs | 988 | 5769-8735 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPRRSILSAA ERDNLLALPD AKEELIRHYT FSDSDLSIIR QRRGPANRLG FAVQLCYLRF PGVILGADEP PFPPLLKLVA DQLKIGIESW GEYGQRGQTR REHLVELQTV FGFQPFTMSH YRQAVQLLTE LAMQTDKGIV LASALIEHLR RQSVILPALN AVERASAEAI TRANWRIYDA LAEPLSDVHR RRLDDLLKRR DNGKTTWLAW LRQSPAKPNS RHMLEHIERL KAWQALDLPS GVERSVHQNR LLKIAREGGQ MTPADLAKFE AQRRYATLVA LAIEGMATVT DEIIDLHDRI LGKLFNAAKN KHQQQFQASG KAINAKVRLY GRIGQALIDA KQSGRDPFAA IEAVMSWDAF AESVTEAQKL AQPDDFDFLH RIGESYATLR GYAPEFLAVL KLRAAPAAKN VLDAIEVLRG MNTDNARKVP ADAPTGFIKP RWQKLVMTDT GIDRRYYELC ALSELKNSLR SGDIWVQGSR QFKDFEDYLV PPAKFASLKQ SSELPLAVAT DCDQYLSERL ELLEAQLATV NRMAAANDLP DAIITESGLK ITPLDAAVPD TAQALIDQTA MILPHVKITE LLLEVDEWTG FTRHFTHLKS GDLAKDKNLL LTTILADAIN LGLTKMAESC PGTTYAKLAW LQAWNTRDET YSTALAELVN AQFRHPFAGH WGDGTTSSSD GQNFRTGSKA ESTGHINPKY GSSPGRTFYT HISDQYAPFH TKVVNVGVRD STYVLDGLLY HESDLRIEEH YTDTAGFTDH VFALMHLLGF RFAPRIRDLG DTKLYIPKGE TAYDALKPMI GGTLNIKHVR AHWDEILRLA TSIKQGTVTA SLMLRKLGSY PRQNGLAVAL RELGRIERTL FILDWLQSVE LRRRVHAGLN KGEARNALAR AVFFNRLGEI RDRSFEQQRY RASGLNLVTA AIVLWNTVYL ERAANALRDH GKPVDDSLLQ YLSPLGWEHI NLTGDYLWRS SAKIGAGKFR PLRPLQPA |
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1. Tuffin IM, Hector SB, Deane SM, Rawlings DE.
Resistance determinants of a highly arsenic-resistant strain of Leptospirillum ferriphilum isolated from a commercial biooxidation tank.. Appl Environ Microbiol. 2006 Mar;72(3):2247-53. doi: 10.1128/AEM.72.3.2247-2253.2006. PubMed ID:
16517682
.