Mobile element type:
Transposon
Name:
TnBth4
Synonyms:
Tn7153
Accession:
TnBth4-CP010092.1
Family:
Tn3
Group:
Tn3
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Bacillus thuringiensis serovar galleriae HD-29
Date of Isolation:
2014
Country:
Czechoslovakia
Molecular Source:
plasmid pBMB126
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGTCTGTG TAGCAATGGA ACCAGATCAC GCAATAAG |
| IRR | 4811-4848 | - | 38 | GGGGTCTGTG TAGCAATGGA ACCAAATCAC GCAATAAG |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res_site_d | TnBth4 | 40-51 | - | 12 | ATGTCCGCTA AT |
| res | TnBth4 | 636-734 | + | 99 | ATGTCCGCTA ATTAATATTA GCGGACATAT GATGCTTTGA AATAAACAAT TGATGTCCGC TAATGTAACT GATAAGATTA GATTAGCATG TCCACTAAT |
| res_site_a | TnBth4 | 636-647 | + | 12 | ATGTCCGCTA AT |
| res_site_b | TnBth4 | 652-663 | - | 12 | ATGTCCGCTA AT |
| res_site_c | TnBth4 | 688-699 | + | 12 | ATGTCCGCTA AT |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| parE | TnBth4 | 119-406 | - | Passenger Gene | Toxin |
| parD | TnBth4 | 351-608 | - | Passenger Gene | Antitoxin |
| tnpI | TnBth4 | 761-1681 | + | Accessory Gene | Resolvase |
| tnpA | TnBth4 | 1753-4758 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | parE | ParE | TnBth4 | 95 | 119-406 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Toxin | ||||||||||
| Sequence Family: | ParE_toxin (Pfam:PF05016) | ||||||||||
| Target: | DNA gyrase | ||||||||||
| Protein Sequence: | MYQGRTQNVN SDYKLIYHKS AVKFIAKQEK GIQKRIAEGL KGLLEIPPEG DIKSMKGYTD LYRLRVGTFR ILFEINHDEK VIYIQAIGNR GDIYK |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | parD | ParD | TnBth4 | 85 | 351-608 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antitoxin | ||||||||||
| Sequence Family: | parD (PDB:4Q2U) | ||||||||||
| Comment: | no PBD | ||||||||||
| Protein Sequence: | MAVRKEELYR LIDCLGHQDE KAAFDFLEFL VQRSKKKPND WEKIDIADSD DEPLSKQELE QLNSEEGYVS GEDAKREFGL QIDLP |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpI | TnpI | TnBth4 | 306 | 761-1681 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Tyrosine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Tyrosine | ||||||||||
| Comment: | belongs tothe phage integrase family of site-specific recombinases | ||||||||||
| Protein Sequence: | MSSIKIISLQ GTSFISDFIS SLSLEGDLHT KTLTEYKSDL KDFVYWFEHL WGNLSEETFF HPTEVTARTI ARYREHMQIT RSLKPATINR RINSIKRYFD WAKQQGIIQT DYSKSIKFVP AVKTSPKQMT DKEEAALMNA VEKYGTLRDK AMIIFMLHTG FRSMEVCDVQ IEDIVMRKRG GNVIVRSGKR NKQREVPLNS TVRFALEEYI GSNNITHSYL FPSSKTGKRL QERAIRHILQ KYMRLANLDG FSAHDLRHRF GYVMAERTPL HRLAQIMGHD NLNTTMIYVR ATQEDLQGEV EKIAWN |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | TnBth4 | 1001 | 1753-4758 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPVDFLTPEQ EEKYGCFCDT PTSEQLAKYF WLDDKDKELI WNRRGEHNQL GFAVQLGTVR FLGTFLSDPT DVPHAVVTYI ADQLGLDAKC FADYRSKRNH WQHMNEIRST YEYKNFTDQP GHWRLIRWLY TRAWLHNDRP SILFDLATAR CIEQKILLPG VSVLTKLVAK VRDRASENLW EKLAELPCTE QRKQLENLLQ SGPKKKKTHL ERLSNPPFTI SITGIKHSLH RLQELRQLEA EYWDTSGIPT KRLQQLARHA VAVRSQAIAR MNDERRIAVL VAFAKIYTQN AQDDVIDILD RYLTDLFAKT YRKEQKERLR TIKDLDKAAR QLREACITLL EHTDPSIHPK VAVFKKVPEK DLIQAVQIVD SLTCPPDQTL AYSELLQYYG TIRKFLPLLM EEIELQATPA GLPILQAWNF VKEHGDSSKK RWRNAPLVGL NTNWSKIVVD KKTRTVNHRA YTFWMLEQVV DALRRHDLYI VGSVKYGDLR AQLLQGEEWK AIRPNVLRSL DWSLDSYESL APLKEELDLA YHQTVENWDN NPAVQIETFA GKQRITLTPL QKLQESETLE ILKKRIQDML PNIDIPQLLL EVNRWTGFMN DFRHISEAKS RINELPISIC ALLISQACNI GLRPLVQDGV PALARDRLTW IEQNYFRAET LTEANTRLVD FHSQLDLANM WGGGEIASAD GLRFFTPVKS VHSGPNPKYF GTGRGVTFYN FTSDQFTGLH GLVIPGTIHD SLYLLQCVLE QDTSLQPKEI MTDTAGYSDI IFGLFGLLGY QFSPRLADVG KSRLWRFDAT SDYGILNPLS KGRIREDLIH RHWEDMLRVA GSLSLNKVNA THLIQALQQN GKPTMLGRAI GEFGRIFKTR YLLLYLNDEN YRRKILTQLN RGEARHSLAR AVFYGKRGEL HQAYRAGQED QLGALGLVVN AIVVWNTRYM ESALQVLRNR GHTLDDNNIA RLSPLGHEHI NIVGRYSFIL PEEIKDGQLR NLTYKEDRLM E |
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