Mobile element type:
Transposon
Name:
Tn6674
Synonyms:
Accession:
Tn6674-MK737778
Family:
Tn554
Group:
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Enterococcus faecalis E1731
Date of Isolation:
2018
Country:
China
Molecular Source:
chromosome
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpA | Tn6674 | 134-1219 | + | Transposase | |
| tnpB | Tn6674 | 1216-3108 | + | Transposase | |
| tnpC | Tn6674 | 3115-3492 | + | Accessory Gene | Helper |
| ANT(9)-Ia (ARO:3002630) | Tn6674 | 3643-4425 | + | Passenger Gene | Antibiotic Resistance |
| ErmA (ARO:3000347) | Tn6674 | 4550-5281 | - | Passenger Gene | Antibiotic Resistance |
| SAM-dependent methyltransferase | Tn6674 | 5672-6334 | + | Passenger Gene | Other |
| fexA (ARO:3002704) | Tn6674 | 8298-9725 | + | Passenger Gene | Antibiotic Resistance |
| optrA (ARO:3003746) | Tn6674 | 10412-12379 | + | Passenger Gene | Antibiotic Resistance |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn6674 | 361 | 134-1219 | + |
|---|
| Class: | Transposase | ||||||||||
| Protein Sequence: | MKVQRIEVEN KPYPLYLLLD KEYQLIEPVM KFIKYLDNTG KSPNTIKAYC YHLKLLYEFM EQRGVILNDI NFELLADFVG WLRYPSASNV IDLQSKKAIR EETTVNTILN VVMSFLDYLS RLGEFKSIDV FKQAKGRNFK GFLHHVNKGR YQKNVLKLRV KKKQIRTLRS KEVKQIIDAC HTKRDKLILM LMYEGGLRIG EVLSLRLEDI VTWDNQIHLT PRDVNVNEAY IKLRKERTIH VSKELMSLYT DYLIYEYSEE LEHDYVFISL KEGYFGKPLK YQSVLDLVRR IVKRTGIEFT SHMLRHTHAT QLIREGWDVA FVQKRLGHAH VQTTLNTYVH LSDQDMKNEF NKYLERKEHK K |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpB | TnpB | Tn6674 | 630 | 1216-3108 | + |
|---|
| Class: | Transposase | ||||||||||
| Protein Sequence: | MNASSKRKII SQSEISKKIA VMNEEMQGFW ANNSWDIRKC PHPSAIELSK NPALRNRWVR FERVKNLWLR TELKYFYFYH LNNGIWNAKT VWIRKGTVIN KMLDFLDLKY PSITSITEVP IEKAMTEYRT YLTKRGVRIT TTNYKITANQ EKTPVKANSY YVTNLKQFME FYENFYFDGE EWDKDVWDRR NLPLPDDKVN PTQYEYTINF KGFRNTYFKQ LVKRYCKLRL NVDSFSYVSD IAQRLKEFFN FLDMKFKQVQ RVHQLTRVEI EAYLSELNMM GIKPSTITGR ISILEGLFST LLRLEWDDVP SKILIYSEDY PKIPRAKPRF IDEFVLEQLN SHLDKLPEYI ATMTMIVQEC GMRISELCTL KKGCLLEDKD GDFFLKYYQW KMKKEHIVPI SKEVALLIKV REDKVSEEFP DSEYLFPRKD GSPLKQETFR GELNKLAYEQ NIVDKSGEIY RFHAHAFRHT VGTRMINNGM PQHIVQKFLG HESPEMTSRY AHIFDETLKN EFTKFQEKLV TNNGDVLDLD EDNEVDDVEL QWFKKNINAQ VLPNGYCRLP VVAGGCPHAN ACLDCTHFCT SKQFLPQHEE QLERTEELLA IAKDKQWQRQ VETNSRVKER LEQIIGSLTG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpC | TnpC | Tn6674 | 125 | 3115-3492 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Helper | ||||||||||
| Sequence Family: | Tn554_family | ||||||||||
| Comment: | target specificity and orientation | ||||||||||
| Protein Sequence: | MDKQVRNTTE IVRLAKQKSK KTREKVDKAI SKFSIEGKVI NFNSIAKEAN VSKSWLYKEH DIRQRIESLR ERQITANVVS KPKKSSRSEE ILIKTLKRRV MELEKENKKL QNQIQKLYGD LYNKE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | ANT(9)-Ia (ARO:3002630) | ANT(9)-Ia | Tn6674 | 260 | 3643-4425 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | ANT(9) (ARO:3000228) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Protein Sequence: | MSNLINGKIP NQAIQTLKIV KDLFGSSIVG VYLFGSAVNG GLRINSDVDV LVVVNHSLPQ LTRKKLTERL MTISGKIGNT DSVRPLEVTV INRSEVVPWQ YPPKREFIYG EWLRGEFENG QIQEPSYDPD LAIVLAQARK NSISLFGPDS SSILVSVPLT DIRRAIKDSL PELIEGIKGD ERNVILTLAR MWQTVTTGEI TSKDVAAEWA IPLLPKEHVT LLDIARKGYR GECDDKWEGL YSKVKALVKY MKNSIETSLN |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | ErmA (ARO:3000347) | ErmA | Tn6674 | 243 | 4550-5281 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | Erm 23S ribosomal RNA methyltransferase (ARO:3000560) | ||||||||||
| Target: | streptogramin B antibiotic (ARO:0000050)||lincosamide antibiotic (ARO:0000017)||streptogramin A antibiotic (ARO:0000034)||macrolide antibiotic (ARO:0000000)||streptogramin antibiotic (ARO:0000026) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3000347 (bitscore: 423) | ||||||||||
| Protein Sequence: | MNQKNPKDTQ NFITSKKHVK EILNHTNINK QDNVIEIGSG KGHFTKELVK MSRWVDSIEI DEDLCQVTQK VVKPFQNIKV IHTDILKFNF PKNKDYKIFG NIPFNISTDI VKKIAFESNS KYSYLIVENG FAKRLQNTKR ALGLLLMVEL DIRVLKKVPR AYFHPKPNVD SVLIVLERHQ PLILKKDYKE YQSFVYKWVN REYRVLFTKN QFRQALKHAN VTNINKLSKE QFLSIFNSYK LFQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | SAM-dependent methyltransferase | SAM-dependent methyltransferase | Tn6674 | 220 | 5672-6334 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | perfect match to reference sequence for Uniprot A0A347WKB4 and Q5HNP8||Synonyms: | ||||||||||
| Protein Sequence: | MNTIESQFDK VAEDYDFVNE LLNDYSFFVS NMSPKKGRAL DIGCGSGLLV EKLASYYDEV VGIDISNQML DLAKSKRQLT NTVYLNMNAE QLNFNEKFDF IVSRTTFHHL DDIASVIQQM KELLNEEGRI VILDNVSEVE TPPTYVYKLG AIQEFLPHCF KFGIKNAIRI YNHNTSKSWL EHLASDKYLS EQNYYDLYEK LLPGCQFHKM GWAMGVVWTK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | fexA (ARO:3002704) | FexA | Tn6674 | 475 | 8298-9725 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic efflux (ARO:0010000) | ||||||||||
| Sequence Family: | major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002) | ||||||||||
| Target: | phenicol antibiotic (ARO:3000387) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002704 | ||||||||||
| Protein Sequence: | MKKDSKSKEM IQSEKRGSTR LLMMVLSLSV LVGSITADSV NPVLPLISKA LEASKSQVSW IVSGIALVLA IGVPIYGRIS DFFELRKLYI FAIMILASGS LLCAIAPNLP LLVLGRMVQG AGMSAIPVLS VIAISKVFPQ GKRGGALGII AGSIGVGTAA GPIFGGVVGQ YLGWNALFWF TFLLAIMIVI GAYYALPTIK PAESVGSNKN FDFIGGLFLG LTVGLLLFGI TQGETSGFSS FSSLTSLIGS VVALVGFIWR IVTAENPFVP PVLFNNKDYV NTVIIAFFSM FAYFAVLVFV PLLVIEVNGL SSGQAGMILL PGGVAVAILS PFVGRLSDRF GDKRLIITGM TLMGLSTLFL STYASGASPL LVSVGVLGVG IAFAFTNSPA NNAAVSALDA DKVGVGMGIF QGALYLGAGT GAGMIGALLS ARRDATEPIN PLYILDAMSY SDAFLAATGA ILIALIAGLG LKKRG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | optrA (ARO:3003746) | OptrA | Tn6674 | 655 | 10412-12379 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target protection (ARO:0001003) | ||||||||||
| Sequence Family: | Miscellaneous ABC-F subfamily ATP-binding cassette ribosomal protection proteins (ARO:3007068) | ||||||||||
| Target: | phenicol antibiotic (ARO:3000387)||oxazolidinone antibiotic(ARO:3000079) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3003746 (Bitscore: 1336) | ||||||||||
| Protein Sequence: | MSEATFAIAS TNAKEDMKMQ YKIINGAVYY DGNMVLENIG IEINDNEKIA IVGRNGCGKT TLLKAIIGEI ELEEGTGESE FQVIKTGNPY ISYLRQMPFE DESISMVDEV RTVFKTLIDM ENKMKQLIDK MENQYDDKII NEYSDISERY MALGGLTYQK EYETMIRSMG FTEADYKKPI SEFSGGQRTK IAFIKILLTK PDILLLDEPT NHLDIETIQW LESYLRSYKS TLVIISHDRM FLNRIVDKVY EIEWGETKCY KGNYSAFEEQ KRENHIKQQK DYDLQQIEIE RITRLIERFR YKPTKAKMVQ SKIKLLQRMQ ILNAPDQYDT KTYMSKFQPR ISSSRQVLSA SELVIGYDTP LAKVNFNLER GQKLGIVGSN GIGKSTLLKT LMGGVAALSG DFKFGYNVEI SYFDQQLAQI SGDDTLFEIF QSEYPELNDT EVRTALGSFQ FSGDDVFRPV SSLSGGEKVR LTLCKLLYKR TNVLILDEPT NHMDIIGKEN LENILCSYQG TIIFVSHDRY FTNKIADRLL VFDKDGVEFV QSTYGEYEKK RMNSEKPFNN IKVEQKVEKN NTVKGDRNSI EKEKVKKEKR IEKLEVLINQ YDEELERLNK IISEPNNSSD YIVLTEIQKS IDDVKRCQGN YFNEWEQLMR ELEVM |
||||||||||
1. Li D, Li XY, Schwarz S, Yang M, Zhang SM, Hao W, Du XD.
Tn6674 Is a Novel Enterococcal optrA-Carrying Multiresistance Transposon of the Tn554 Family.. Antimicrob Agents Chemother. 2019 Aug 23;63(9):e00809-19. doi: 10.1128/AAC.00809-19. Print 2019 Sep. PubMed ID:
31209008
.