Mobile element type:
Transposon
Name:
Tn6346
Synonyms:
Accession:
Tn6346-KM659090
Family:
Tn3
Group:
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Achromobacter sp. LM16
Date of Isolation:
2014
Country:
Poland
Molecular Source:
plasmid pLM16A1
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRR | 1-38 | + | 38 | GGGGTCGTCT CAGAAAACGG AAAATAAAGC ACGCTAAG |
| repeat i4 | 10-28 | + | 19 | TCAGAAAACG GAAAATAAA |
| repeat i4 | 8203-8221 | - | 19 | TCAGAAAACG GAAAATAAA |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res_site_I | Tn6346 | 4522-4558 | + | 37 | GCTACCGTCA GATTGAGGCA TACCCTAACT TGGCGTC |
| res | Tn6346 | 4574-4650 | + | 77 | AAATCGTCAG AATAGAGTTG CCTTCCGAAT TGATTGACAT ACGCCGTCAA GGGTCATAGA TTTCTTCCTG ACACATT |
| res_site_II | Tn6346 | 4574-4616 | + | 43 | AAATCGTCAG AATAGAGTTG CCTTCCGAAT TGATTGACAT ACG |
| res_site_III | Tn6346 | 4621-4650 | + | 30 | CAAGGGTCAT AGATTTCTTC CTGACACATT |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| merR | Tn6346 | 34-468 | - | Passenger Gene | Heavy Metal Resistance |
| merT | Tn6346 | 540-890 | + | Passenger Gene | Heavy Metal Resistance |
| merP | Tn6346 | 903-1178 | + | Passenger Gene | Heavy Metal Resistance |
| merA | Tn6346 | 1250-2935 | + | Passenger Gene | Heavy Metal Resistance |
| merD | Tn6346 | 2953-3318 | + | Passenger Gene | Heavy Metal Resistance |
| merE | Tn6346 | 3315-3551 | + | Passenger Gene | Heavy Metal Resistance |
| urfM | Tn6346 | 3548-4537 | + | Passenger Gene | Other |
| tnpR | Tn6346 | 4746-5228 | + | Accessory Gene | Resolvase |
| tnpA | Tn6346 | 5231-8197 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR | MerR | Tn6346 | 144 | 34-468 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | activator-repressor of mer operon | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MENNLENLTI GVFAKAAGVN VETIRFYQRK GLLLEPDKPY GSIRRYGEAD VTRVRFVKSA QRLGFSLDEI AELLRLEDGT HCEEASSLAE HKLKDVREKM ADLARMEAVL SELVCACHAR RGNVSCPLIA SLQGGASLAG SAMP |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | Tn6346 | 116 | 540-890 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | cytosolic mercuric ion transport protein | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MSEPKTGRGA LFTGGLAAIL ASACCLGPLV LIALGFSGAW IGNLAVLDPY RPIFIGVALV ALFFAWRRIY RQAAACKPGE VCAIPQVRAT YKLIFWIVAA LVLVALGFPY VMPFFY |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | Tn6346 | 91 | 903-1178 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercury transport | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MKKLFASLAL AAVVAPVWAA TQTVTLSVPG MTCSACPITV KKAISKVEGV SKVDVTFETR QAVVTFDDAK TSVQKLTKAT ADAGYPSSVK Q |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA | MerA | Tn6346 | 561 | 1250-2935 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercuric ion reductase | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MTHLKITGMT CDSCAAHVKE ALEKVPGVQS ALVSYPKGTA QLAIVPGTSP DALTAAVAGL GYKATLADAP LADNRVGLLD KVRGWMAAAE KHSGNEPPVQ VAVIGSGGAA MAAALKAVEQ GAQVTLIERG TIGGTCVNVG CVPSKIMIRA AHIAHLRRES PFDGGIAATV PTIDRSKLLA QQQARVDELR HAKYEGILGG NPAITVVHGE ARFKDDQSLT VRLNEGGERV VMFDRCLVAT GASPAVPPIP GLKESPYWTS TEALASDTIP ERLAVIGSSV VALELAQAFA RLGSKVTVLA RNTLFFREDP AIGEAVTAAF RAEGIEVLEH TQASQVAHMD GEFVLTTTHG ELRADKLLVA TGRTPNTRSL ALDAAGVTVN AQGAIVIDQG MRTSNPNIYA AGDCTDQPQF VYVAAAAGTR AAINMTGGDA ALDLTAMPAV VFTDPQVATV GYSEAEAHHD GIETDSRTLT LDNVPRALAN FDTRGFIKLV IEEGSHRLIG VQAVAPEAGE LIQTAALAIR NRMTVQELAD QLFPYLTMVE GLKLAAQTFN KDVKQLSCCA G |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD | MerD | Tn6346 | 121 | 2953-3318 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | secondary regulatory protein | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MNAYTVSRLA LDAGVSVHIV RDYLLRGLLR PVACTPGGYG LFDDAALQRL CFVRAAFEAG IGLDALARLC RALDAADGDE AAAQLALLRQ FVERRREALA DLEVQLATLP TEPAQHAESL P |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merE | MerE | Tn6346 | 78 | 3315-3551 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercury transport | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MNNPERLPSE THKPITGYLW GGLAVLTCPC HLPILAVVLA GTTAGAFLGE HWVIAALGLT GLFLLSLSRA LRAFRERE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | urfM | UrfM | Tn6346 | 329 | 3548-4537 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Function: | possible diguanylate phosphodiesterase | ||||||||||
| Sequence Family: | EAL (Pfam:PF00563)||DUF3330 (Pfam:PF11809) | ||||||||||
| Comment: | similar to urfM from E.coli | ||||||||||
| Protein Sequence: | MSAFRPDGWT TPELAQAVER GQLELHYQPV VDLRSGGIVG AEALLRWRHP TLGLLPPGQF LPVVESSGLM PEIGAWVLGE ACRQMRDWRM LAWRPFRLAV NVSASQVGPD FDGWVKGVLA DAELPAEYLE IELTESVAFG DPAIFPALDA LRQIGVRFAA DDFGTGYSCL QHLKCCPIST LKIDQSFVAG LANDRRDQTI VHTVIQLAHG LGMDVVAEGV ETSASLDLLR QADCDTGQGF LFAKPMPAAA FAVFVSQWRG ATMNASDSTT TSCCVCCKEI PLDAAFTPEG AEYVEHFCGL ECYQRFEARA KTGNETDADP NACDSLPSD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn6346 | 160 | 4746-5228 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | VDKVFTDKAS GKDTRRPELE RLLAFVREGD TVVVHSMDRL ARNLDDLRRL VQGLTQRGVR IEFLKEHLTF TGEDSPMANL MLSVMGAFAE FERALIRERQ REGIALAKQR GAYRGRKKSL SSERIAELRQ RVEAGEQKTK LAREFGISRE TLYQYLRTDQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn6346 | 988 | 5231-8197 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPRRSILSAA ERESLLALPD TKDELIRHYT FSETDLSIIR QRRGPANRLG FAVQLCYLRF PGVILGVDEP PFPPLLKLVA DQLKVSVESW DEYGQREQTR REHLVELQTV FGFQPFTMGH YRQAVQLLTE MALQTDKGIV LASTLIEHLR QQSVILPALN AVERASAEAI TRANRRIYDA LAEPLSDAHR RRLDDLLKRR DNGKTTWLAW LRQSPVKPNS RHMLEHIERL KAWQALDLPS GIERSVHQNR LLKIAREGGQ MTPADLAKFE AQRRYATLVA LAIEGMATVT DEIIDLHDRI LGKLFNAAKN KHQQQFQASG KAINAKVRLF GRIGQALIEA KQAGRDPFAA IEAVMSWDAF AESVTEAQKL AQPEDFDFLH RIGESYATLR RYAPEFLAVL KLRAAPAAKD VLDAIEVLRG MNSDNARKVP ADAPTEFIKP RWQKLVMTDT GIDRRYYELC ALSEMKNALR SGDIWVQGSR QFKDFEDYLV PPAKFASLKQ ASELPLAVAT DCNRYLNDRL TLLETQLATV NRMATANELP DAIITESGLK ITPLDAAVPD TAQALIDQTA MILPHVKITE LLLEVDEWTG FTRHFAHLKS GDPAKDKNLL LTTILADAIN LGLTKMAESC PGTTYAKLAW LQAWHIRDET YGAALADLVN AQFRHPFAEH WGDGTTSSSD GQNFRTGSKA ESTGHINPKY GSSPGRTFYT HISDQYAPFH TKVVNVGVRD STYVLDGLLY HESDLRIEEH YTDTAGFTDH VFALMHLLGF RFAPRIRDLG DTKLYIPKGD AAYDALKPMI GGTLNIKHVR AHWDEILRLA TSIKQGTVTA SLMLRKLGSY PRQNGLAVAL RELGRIERTL FILDWLQSVE LRRRVHAGLN KGEARNALAR AVFFNRLGEI RDRSFEQQRY RASGLNLVTA AVVLWNTVYL ERAAHALRGN GHAVDDALLQ YLSPLGWEHI NLTGDYLWRS SAKIGAGKFR PLRPLQPA |
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1. Zhan Z, Hu L, Jiang X, Zeng L, Feng J, Wu W, Chen W, Yang H, Yang W, Gao B, Yin Z, Zhou D.
Plasmid and chromosomal integration of four novel blaIMP-carrying transposons from Pseudomonas aeruginosa, Klebsiella pneumoniae and an Enterobacter sp.. J Antimicrob Chemother. 2018 Nov 1;73(11):3005-3015. doi: 10.1093/jac/dky288. PubMed ID:
30351436
.