Mobile element type:
Transposon
Name:
Tn6332
Synonyms:
TnMERI1
Accession:
Tn6332-LC155216.1
Family:
Tn3
Group:
Tn4430
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Bacillus megaterium MB1
Date of Isolation:
2016
Country:
Japan
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGTCTTAC CAACAAAACG CGGAAAACAT ACGCTAAG |
| IRR | 11471-11508 | - | 38 | GGGGTCCTAC CAACAAAACG CGGAAAACAT ACGCTAAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| merB1 | Tn6332 | 639-1295 | - | Passenger Gene | Heavy Metal Resistance |
| merB2 | Tn6332 | 1445-2074 | - | Passenger Gene | Heavy Metal Resistance |
| merR2 | Tn6332 | 2071-2460 | - | Passenger Gene | Heavy Metal Resistance |
| merA | Tn6332 | 3361-5256 | - | Passenger Gene | Heavy Metal Resistance |
| merP | Tn6332 | 5275-5625 | - | Passenger Gene | Heavy Metal Resistance |
| merT | Tn6332 | 5673-5969 | - | Passenger Gene | Heavy Metal Resistance |
| merE | Tn6332 | 5966-6235 | - | Passenger Gene | Heavy Metal Resistance |
| merR1 | Tn6332 | 6250-6648 | - | Passenger Gene | Heavy Metal Resistance |
| merB3 | Tn6332 | 6726-7454 | - | Passenger Gene | Heavy Metal Resistance |
| tnpR | Tn6332 | 7887-8444 | + | Accessory Gene | Resolvase |
| tnpA | Tn6332 | 8459-11410 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merB1 | MerB1 | Tn6332 | 218 | 639-1295 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | organomercurial lyase | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MKTEIQEIVT RLDQQSNKGE GGESMKWLFR PLLQMLAGGE SVTIEDMATT TGKPVEEVKK VLQSLPSVEI DEQGRVVGLG LTLIPTPHHF TVDGKQLYAW CALDTLIFPA LIGRSVNIES PCHSTGEPIR LNVEPDHIVS VEPSTAVVSI VTPDDMSSIR TAFCNEVHFF SSPNAAEDWL DQHPGGKVLS VEDAFELGRL MGTRYEESRP ANGSCCDI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merB2 | MerB2 | Tn6332 | 209 | 1445-2074 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MKNKTELKKF YELLLAKLPK ESVPILRTIF FSIRDGQAVT ESSLINQTGI NTKTVQSVVK ILAQRQMIVR EADQKIVGAL GLSIIPTTNQ IHLGGRTLFA WCAISTLELS TALVADVDIH SRCAYTGEPI EVTVRNGKLA KTTPDSTVIW TVPFDSEAPW AGGTCKQIHY FSSVEHANKW KEEHPKLQGE IMTLEQALSF GNELKKFLS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR2 | MerR2 | Tn6332 | 129 | 2071-2460 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MEDLTIGQLA QQTGVSRKAI RLYEEKELIL PSIKRSEGNY RVYNQEHVFC INGIKQLRSL GVSLEEMKDL IVIFEKNTVE IEPHLQHLLK DKLTKIDEQI NELQKLRKHI ESYLDSPKEA FNQMEGFKQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA | MerA | Tn6332 | 631 | 3361-5256 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | mercury reductase | ||||||||||
| Protein Sequence: | MKKYRVNVQG MTCSGCEQHV AVALENMGAK AIEVDFRRGE AVFELPDDVK VEDAKNAIAD ANYHPGEAEE FQSEQKTNLL KKYRLNVEGM TCTGCEEHIA VALENAGAKG IEVDFRRGEA LFELPYDVDI DIAKTAITDA QYQPGEAEEI QVQSEKRTDV SLNDEGNYDY DYIIIGSGGA AFSSAIEAVA LNAKVAMIER GTVGGTCVNV GCVPSKTLLR AGEINHLAKN NPFVGLHTSA SNVDLAPLVK QKNDLVTEMR NEKYVNLIDD YGFELIKGEA KFVNENTVEV NGNQITAKRF LIATGASSTA PNIPGLDEVD YLTSTSLLEL KKVPNRLTVI GSGYIGMELG QLFHNLGSEV TLIQRSERLL KEYDPEISEA ITKALTEQGI NLVTGATYER VEQDGDIKKV HVEINGKKRI IEAEQLLIAT GRKPNTESLN LHAAGVEVGS RGEIVIDDYL KTTNSRIYSA GDVTLGPQFV YVAAYEGGLA ARNAIGGLNQ KVNLEVVPGV TFTSPSIATV GLTEQQAKEK GYEVKTSVLP LDAVPRALVN RETTGVFKLV ADAKTLKVLG AHVVAENAGD VIYAATLAVK FGLTVGDLRE TMAPYLTMAE GLKLAVLTFD KDVSKLSCCA G |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | Tn6332 | 116 | 5275-5625 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MMLLSLMLVV SACSNEQEVQ KTEVSKSEVK TTAMNSEKDV KAITEAEKTG TFMVIAGMDC CPPSVVEDAI AQVEGAGKTA IKVNGSTAEV TVSFDDTKTN LDAIKTEVSD LGLPVE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | Tn6332 | 98 | 5673-5969 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MKEKVSQVAT VFSAFVMAGC CLGPLILIPL GLTGFAGAIA FYSLKYRLLF SIVTIVLLAY SFYMVYGRKG KRKSSVIGLW ITTFLVFTMF LFLFSVES |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merE | MerE | Tn6332 | 89 | 5966-6235 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MKNIIKSSGW FLVALITCPC HLFLLLPLIA GTALGSYFTE FKNVIFIMMG LLFVFALFKG WRKLDPETKE ETKKDTTTHD CCSMEKFKS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR1 | MerR1 | Tn6332 | 132 | 6250-6648 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MKFRIGELAD KCGVNKETIR YYERLGLIPE PERTEKGYRM YSQQTVDRLH FIKRMQELGF TLNEIDKLLG VVDRDEAKCR DMYDFTILKI EDIQRKIEDL KRIERMLMDL KERCPENKDI YECPIIETLM KK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merB3 | MerB3 | Tn6332 | 242 | 6726-7454 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | organomercurial lyase | ||||||||||
| Protein Sequence: | MNQNHLNTSL KDKVLQSLGL PEEGFEGKIR LLSPSENSIR LDILLFMAEG KIVNINDLTA TEEQIDVQSA LQRLRELDLI HWDQNSGDVN VAYPFSGVPT PHRVTLAGML PAYSMCAIDA LGIPSMFTDA VIESECAFCG EKITIDVKNN MPVANPDTVV VGLGTTDAAD TNACCDSSCE SNEPTSISTS CCPAIQFYCS EEHWQKANEK NSTTAKDKLT LAEAFEVGAA VFGGTLSGSK GK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn6332 | 185 | 7887-8444 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MKFGYARVST QDQSLSLQLD ALAHYGVDQI FEEKESGKMK NRPQLDELLK VLRKDDTVIV YKLDRISRST KHLIELMEYF ESKEIHFVSI QDKIDTTTAM GRFFFRMLAS IAELERDIIS ERTKDGLTAA RARGRNGGRP KVDLKKIELA IKMYESKDYS LSQIKTATGI GATTLYRYLE KKKDI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn6332 | 983 | 8459-11410 | + |
|---|
| Class: | Transposase | ||||||||||
| Function: | transposase activity (GO:0004803) | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MRGKELLTED QRTEFVRIPA DMSESELETY YTFSQYDLEI IKRHRRDHNR LGFAVQLCVL RYPGWSLSDV GPIPDYIIEY IASQIDVNPN SFSLYAQREP TKHEHMEEIR QVYGYQNFSL GNYRKAAQFL LNHALQNGNS MYLLRTAQEE LRNQKIILPG MTTIERLVWE TRQRAEERIF KSLTCTLSKW QKQKLDKLID PFVDNRKTPL AWLRELPGQS SPEAFLKVIK RLEYIRELKL EINTEQIHPN RLLQLSRIGA RYEPHSFRRF KEMKKYAILV AYLVTLSQDL IDQAIEIHDR QMMILQSKGR KTQEEMQKEN GKAVNEKVVH FADIGAALIQ ARNEGLDPFN AIEMIMPWDK IVSSVEEAQK LARPMDYDYL DLLENRFSYL RKYTPTFLKS LEFRSTQGTE PLLQALKTLN EINESGKRKI PNDAPVDFIP KRWKKHVYGD DGIINRHYYE MAALTELKNH IRSGDISVVG SRLHKDFEEY LVPKKDWTTT RLTETKLAVR SSADEYIEER RKSLAQRLTW VSNNLDSLEG VNIEKAKIRV DRLEKNTPEE ARAFSLSLYN MLPRIKLTDL LMEVAHWTGF DEMLIHASTN RPPKGEEKVV LMAALMAMGT NIGLTKMAEA TPGVTYHQMA NAAQWRLFDD AISRAQATLV NFQHKLKLAS YWGDGSTSSS DGMRVQVGVS SLHADANPHY GSGKGATIYR FTSDQFSSFY TKVINTNARD AVHVIDGLLH HETDLNIEEH FTDTAGYADS VFGLSHLLGF RFAPRLRDLA DSKLFTIQTP NEFPKLENIL RGRINTKIIQ ENFDDVLRVA HSIREGKVSG SLIMGKLGSY ARQNKLATTL REMGRIEKTI FILDYISNET LRRRIQRGLN KGEAMNGLAR ALFFGKRGEL RERGIQDQLQ RASALNILIN AISVWNTVYL TEATKLLKEK GNLREDLLKH VSPLGWEHIN FLGEYNFDAS KVASLHSLRP LIQ |
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1. Matsui K, Yoshinami S, Narita M, Chien MF, Phung le T, Silver S, Endo G.
Mercury resistance transposons in Bacilli strains from different geographical regions.. FEMS Microbiol Lett. 2016 Mar;363(5):fnw013. doi: 10.1093/femsle/fnw013. Epub 2016 Jan 21. PubMed ID:
26802071
.