Mobile element type:
Transposon
Name:
Tn6330
Synonyms:
Accession:
Tn6330-KX772391
Family:
Compound Transposon
Group:
ISApl1
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Escherichia coli strain EHS30-1
Date of Isolation:
2016
Country:
China
Molecular Source:
plasmid pEHS30-1
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpA | ISApl1 | 89-1063 | + | Transposase | |
| MCR-1.1 (ARO:3003689) | Tn6330 | 1259-2884 | + | Passenger Gene | Antibiotic Resistance |
| pap2 | Tn6330 | 2956-3678 | + | Passenger Gene | Other |
| tnpA | ISApl1 | 3768-4742 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | ISApl1 | 324 | 89-1063 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MMSTSYRHLT INEREKIMIL LAQGKKQAEI AKALGRSSST ISRELKRHAL ESYSATNAQN SYLKHRQNSK AQRKLEQPEY FNLVQEKFLT ENWSPEQISA RLKLEKSELS ISYSTIYRGI YSGLFDIGER KASRKLRHKG KTRHTKNHHE KRGKIQISNH LNDRPISAQN RSRFGHWEAD TVLGKAGGAC LLTLTERKSR FELVKKIPAK KAEAVQKAMI ELLDSHILRS ITPDRGKEFA QHRLVTEALG VEFYFPEPHQ PWTRGTNENT NGLLREYFPK HQDINQWSEV DIQQVINKLN LRPRKCLGWK TPYEVYFKKS LHLV |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | MCR-1.1 (ARO:3003689) | MCR-1.1 | Tn6330 | 541 | 1259-2884 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | MCR phosphoethanolamine transferase (ARO:3004268) | ||||||||||
| Target: | peptide antibiotic (ARO:3000053) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3003689||colistin resistance | ||||||||||
| Protein Sequence: | MMQHTSVWYR RSVSPFVLVA SVAVFLTATA NLTFFDKISQ TYPIADNLGF VLTIAVVLFG AMLLITTLLS SYRYVLKPVL ILLLIMGAVT SYFTDTYGTV YDTTMLQNAL QTDQAETKDL LNAAFIMRII GLGVLPSLLV AFVKVDYPTW GKGLMRRLGL IVASLALILL PVVAFSSHYA SFFRVHKPLR SYVNPIMPIY SVGKLASIEY KKASAPKDTI YHAKDAVQAT KPDMRKPRLV VFVVGETARA DHVSFNGYER DTFPQLAKID GVTNFSNVTS CGTSTAYSVP CMFSYLGADE YDVDTAKYQE NVLDTLDRLG VSILWRDNNS DSKGVMDKLP KAQFADYKSA TNNAICNTNP YNECRDVGML VGLDDFVAAN NGKDMLIMLH QMGNHGPAYF KRYDEKFAKF TPVCEGNELA KCEHQSLINA YDNALLATDD FIAQSIQWLQ THSNAYDVSM LYVSDHGESL GENGVYLHGM PNAFAPKEQR SVPAFFWTDK QTGITPMATD TVLTHDAITP TLLKLFDVTA DKVKDRTAFI R |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | pap2 | Pap2 | Tn6330 | 240 | 2956-3678 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Sequence Family: | PAP2 (PF01569) | ||||||||||
| Comment: | truncated | ||||||||||
| Protein Sequence: | MPYLSNKRLL AEMSIALVMA IVATLTLEHS QIDLMVADWF YLGMGHWMVA KQAFLPDLLL YSGLKKLLMA MLIYLLVATI CRAYHEKKGN AITAKWLVPV TKFRVRELAY LVLTLILVPT VVASLKAYTH VVCPVHLTIF DGTLPYLPML DSMRNTIPDK CFPAAHASSG FALFAFAFAP SLRRRRGAII IVVMALGWAM GCYKMIIGDH FLSHTVVSMM LAWAMSAGLA WVFFKKGEQV |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | ISApl1 | 324 | 3768-4742 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MMSTSYRHLT INEREKIMIL LAQGKKQAEI AKALGRSSST ISRELKRHAL ESYSATNAQN SYLKHRQNSK AQRKLEQPEY FNLVQEKFLT ENWSPEQISA RLKLEKSELS ISYSTIYRGI YSGLFDIGER KASRKLRHKG KTRHTKNHHE KRGKIQISNH LNDRPISAQN RSRFGHWEAD TVLGKAGGAC LLTLTERKSR FELVKKIPAK KAEAVQKAMI ELLDSHILRS ITPDRGKEFA QHRLVTEALG VEFYFPEPHQ PWTRGTNENT NGLLREYFPK HQDINQWSEV DIQQVINKLN LRPRKCLGWK TPYEVYFKKS LHLV |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| ISApl1-EF407820 | ISApl1 | insertion sequence | 3-1072 | + | 1070 |
| ISApl1-EF407820 | ISApl1 | insertion sequence | 3682-4751 | + | 1070 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRL | ISApl1 | 3-29 | + | 27 | GCTGAATTTA CAATCCAAGT GCAACAA |
| IRR | ISApl1 | 1046-1072 | - | 27 | CTTGAATTGT CAAACCAAGT GCAACGA |
| IRL | ISApl1 | 3682-3708 | + | 27 | GCTGAATTTA CAATCCAAGT GCAACAA |
| IRR | ISApl1 | 4725-4751 | - | 27 | CTTGAATTGT CAAACCAAGT GCAACGA |
1. Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, Doi Y, Tian G, Dong B, Huang X, Yu LF, Gu D, Ren H, Chen X, Lv L, He D, Zhou H, Liang Z, Liu JH, Shen J.
Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study.. Lancet Infect Dis. 2016 Feb;16(2):161-8. doi: 10.1016/S1473-3099(15)00424-7. Epub 2015 Nov 19. PubMed ID:
26603172
.
2. Li R, Xie M, Zhang J, Yang Z, Liu L, Liu X, Zheng Z, Chan EW, Chen S. Genetic characterization of mcr-1-bearing plasmids to depict molecular mechanisms underlying dissemination of the colistin resistance determinant.. J Antimicrob Chemother. 2017 Feb;72(2):393-401. doi: 10.1093/jac/dkw411. Epub 2016 Sep 28. PubMed ID: 28073961 .
3. Snesrud E, McGann P, Chandler M. The Birth and Demise of the ISApl1-mcr-1-ISApl1 Composite Transposon: the Vehicle for Transferable Colistin Resistance.. mBio. 2018 Feb 13;9(1):e02381-17. doi: 10.1128/mBio.02381-17. PubMed ID: 29440577 .
4. He YZ, Li XP, Miao YY, Lin J, Sun RY, Wang XP, Guo YY, Liao XP, Liu YH, Feng Y, Sun J. The ISApl1 (2) Dimer Circular Intermediate Participates in mcr-1 Transposition.. Front Microbiol. 2019 Jan 22;10:15. doi: 10.3389/fmicb.2019.00015. eCollection 2019. PubMed ID: 30723461 .
5. Li R, Chen K, Chan EW, Chen S. Characterization of the stability and dynamics of Tn6330 in an Escherichia coli strain by nanopore long reads.. J Antimicrob Chemother. 2019 Jul 1;74(7):1807-1811. doi: 10.1093/jac/dkz117. PubMed ID: 30927434 .
6. Liu X, Li R, Zheng Z, Chen K, Xie M, Chan EW, Geng S, Chen S. Molecular Characterization of Escherichia coli Isolates Carrying mcr-1, fosA3, and Extended-Spectrum-beta-Lactamase Genes from Food Samples in China.. Antimicrob Agents Chemother. 2017 May 24;61(6):e00064-17. doi: 10.1128/AAC.00064-17. Print 2017 Jun. PubMed ID: 28373188 .
2. Li R, Xie M, Zhang J, Yang Z, Liu L, Liu X, Zheng Z, Chan EW, Chen S. Genetic characterization of mcr-1-bearing plasmids to depict molecular mechanisms underlying dissemination of the colistin resistance determinant.. J Antimicrob Chemother. 2017 Feb;72(2):393-401. doi: 10.1093/jac/dkw411. Epub 2016 Sep 28. PubMed ID: 28073961 .
3. Snesrud E, McGann P, Chandler M. The Birth and Demise of the ISApl1-mcr-1-ISApl1 Composite Transposon: the Vehicle for Transferable Colistin Resistance.. mBio. 2018 Feb 13;9(1):e02381-17. doi: 10.1128/mBio.02381-17. PubMed ID: 29440577 .
4. He YZ, Li XP, Miao YY, Lin J, Sun RY, Wang XP, Guo YY, Liao XP, Liu YH, Feng Y, Sun J. The ISApl1 (2) Dimer Circular Intermediate Participates in mcr-1 Transposition.. Front Microbiol. 2019 Jan 22;10:15. doi: 10.3389/fmicb.2019.00015. eCollection 2019. PubMed ID: 30723461 .
5. Li R, Chen K, Chan EW, Chen S. Characterization of the stability and dynamics of Tn6330 in an Escherichia coli strain by nanopore long reads.. J Antimicrob Chemother. 2019 Jul 1;74(7):1807-1811. doi: 10.1093/jac/dkz117. PubMed ID: 30927434 .
6. Liu X, Li R, Zheng Z, Chen K, Xie M, Chan EW, Geng S, Chen S. Molecular Characterization of Escherichia coli Isolates Carrying mcr-1, fosA3, and Extended-Spectrum-beta-Lactamase Genes from Food Samples in China.. Antimicrob Agents Chemother. 2017 May 24;61(6):e00064-17. doi: 10.1128/AAC.00064-17. Print 2017 Jun. PubMed ID: 28373188 .