Mobile element type:
Transposon
Name:
Tn6294
Synonyms:
Accession:
Tn6294-LC015492.1
Family:
Tn3
Group:
Tn4430
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Paenibacillus sp. EOA1
Date of Isolation:
2016
Country:
Antartic
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGTATTAC CAACAAAACG CGGAAAACAT ACGCTAAG |
| IRR | 8537-8574 | - | 38 | GGGGTATTAC CAACAAAACG CGGAAAACAT ACGCCAAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| merB1 | Tn6294 | 320-970 | - | Passenger Gene | Heavy Metal Resistance |
| merR2 | Tn6294 | 1003-1440 | - | Passenger Gene | Heavy Metal Resistance |
| merA | Tn6294 | 1714-3354 | - | Passenger Gene | Heavy Metal Resistance |
| merP | Tn6294 | 3370-3684 | - | Passenger Gene | Heavy Metal Resistance |
| merT | Tn6294 | 3726-4028 | - | Passenger Gene | Heavy Metal Resistance |
| merE | Tn6294 | 4025-4294 | - | Passenger Gene | Heavy Metal Resistance |
| merR1 | Tn6294 | 4308-4706 | - | Passenger Gene | Heavy Metal Resistance |
| tnpR | Tn6294 | 4954-5523 | + | Accessory Gene | Resolvase |
| tnpA | Tn6294 | 5525-8476 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merB1 | MerB1 | Tn6294 | 216 | 320-970 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | organomercurial lyase | ||||||||||
| Protein Sequence: | MKTEIQELVS LFDQNKGEGG GSMKWLFRPL LQMLTKGEPV TVEDIATTTG KPLDEVKDIL PTLSSAELDE QGRVVGFGLT LIPTPHRFEV DEKQLYAWCA LDTLMFPELI GHMVHIESPC YCTGKPIRLT VEPDRVLSLE PTTAVVSIVT PDDTSSVRSS FCNEVHFFST ESAAQNWLNQ HPNGKVYPVK EAFELGRLLG KRYEEPGPTN ESCCDI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR2 | MerR2 | Tn6294 | 145 | 1003-1440 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MEQFTIGQLA QQTGVSRKAI RLYEEKGLIL PSIKRSEGNY RVYNQEHVFC INGIKQLRSL GVSLEEMTDL VVIFEKKPVE VEPRLQHLLK EKLTMIDEHI KDLQKLRQHV GAFLDSPKDV FNQVYLPKNF TIECRYNHVS NIEKQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA | MerA | Tn6294 | 546 | 1714-3354 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | mercury reductase | ||||||||||
| Protein Sequence: | MKKYRVTVQG MTCTGCEEHV AVALENMGAK GIEVDFRRGE AVFELTNDVE VETAKKAIAE AKYQPGEVEE LQPQEMVQLG DEGDYDYIII GSGGAAFSSA IEAVKYGAKV AIIERGTVGG TCVNIGCVPS KTLLRAGEIN HLAKNNPFIG LHTSAGDVDL APLIKQKNEL VTDLRNSKYV DLIDDYGFEL IKGEAKFVDE KTVEVNGMQL SAKRFLIATG ASPAVPNIPG LDEVDYLTST TLLELKKVPK RLTVIGSGYI GMELGQLFHN LGSEVTLMQR SPRLLKEYDP EVSEAITQAL TEQGVNLVTG ASFERIEQDG DIKKVHVEVN GKKRIIEADQ LLAATGRTPN TATLNLQDAG VEVGPRGEIV IDEYSKTTNS RIYAAGDVTL GPQFVYVAAY QGGVAAGNAI GGLNKKLNLE VVPGVTFTAP AIATVGLTEQ QAKEKGYEVK TSVLPLDAVP RALVNRETTG VFKLVADEKT LKVLGAHVVA ENAGDVIYAA TLAVKFGLTV EDLRETMAPY LTMAEGLKLA ALTFDKDVSK LSCCAG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | Tn6294 | 104 | 3370-3684 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MMLLSLLLVV SACSNEKEAQ NTIDAKQEVK TTIETTKTGT FTVLGMDCCP PSVVESVLEQ VDGVSKTAIK VSGSKGEVTV SFDDKKTDLQ AIKTAVSNFG LGVE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | Tn6294 | 100 | 3726-4028 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MKEKVSQVAT VFSAFVMAAC CLGPLIFIPL GLTGFAGALA FYSLKYRLLF SIVTIILLAY SFYMVYGRKG KRKSSIIGLW ITTFFVFCMF LFLFLVEGGL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merE | MerE | Tn6294 | 89 | 4025-4294 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MKNTIKSSGW FLVALITCPC HLFLLLPLVA GTALGSYFTD FKDVTFIVMG LLFVFALFKG WRKLDPETKN ETKKETKTHD CCSMERFKS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR1 | MerR1 | Tn6294 | 132 | 4308-4706 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | mercury-responsive transcriptional regulator protein | ||||||||||
| Protein Sequence: | MHFRVGEIAE KCNVNKETIR YYERLGLIPE PDRTEKGYRM YSQQTVDRLN FIKRMQELGF SLNEIDKLLG VVDRDEAKCR DMYDFTVLKI EDIQHKIQDL KRIEQVLMDL KERCPENKDI YECPIIETLM KK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn6294 | 189 | 4954-5523 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MKFGYARVST QDQSLSLQLD ALTHYGVDQT FEEKESGKRK NRPQLDELLK VLRKGDTVVV YKLDRISRST KHLIELMEHF EAKGIHFVSI QDKIDTTTAM GRFFFRMLAS IAELERDIIS ERTKDGLTAA RARGRNGGRP KVDLKKIELA IKMYESKDYS LSQIKTATGI GATTLYRYLE KKKISSKEL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn6294 | 983 | 5525-8476 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MRGKELLTED QRTEFVRIPA DMSESELETY YTFSQYDLEI IKRHRRDHNR LGFAVQLCVL RYPGWSLSDV GPIPDYIIEY IARQINVNPN SFSLYAQREP TKHEHMEEIR QVYGYENFSL GNYRKVAQFL LNHALQNGNS MYLLRTAQEE LRNQKIILPG MTTIERLVWE ARQRAEERIF KSLTCTLSKW QKQKLDKLID PFVDNRKTPL AWLRELPGQS SPEAFLKVIK RLEYIRDLKL EINSEQIHPN RLLQLSRIGA RYEPHSFRRF KEKKKYAILV SYLVTLSQDL IDQAIEIHGR QMMILQSKGR KTQEEMQKEN GKAVNEKVVH FADIGAALIQ ARNEGLDPFN AIEMIMPWDK IVSSVEEAQK LARPMDYDYL DLLENRYSYL RKYTPTFLKS LEFRSTQGTE PLLQALKTLN EINESGKRKI PNDAPVDFIP KRWKKHVYGD DGIINRHYYE MAALTELKNH IRSGDISVVG SRLHKDFEEY LVPKEDWKTT RLTETKLAVR SSADEYLEER RKSLAQRLTW VSNNLDSLEG VNIEKAKIRL DRLEKNTPEE ARAFSLSLYN MLPRIKLTDL LMEVAHWTGF DEMLIHASTN RPPKGEEKVV LMAALMAMGT NIGLTKMAEA TPGVTYHQMA NAAQWRLFDD AISRAQATLV NFQHKLKLAS YWGDGSTSSS DGMRVQVGVS SLHADSNPHY GSGKGATIYR FTSDQFSSFY TKVINTNARD AVHVIDGLLH HETDLNIEEH FTDTAGYADS VFGLSHLLGF RFAPRLRDLA DSKLFTIQTP NEFPKLENIL RGRINTKIIQ ENFDDVLRVA HSIREGKVSG SLMMGKLGSY ARQNKLATTL REMGRIEKTI FILDYISNET LRRRIQRGLN KGEAMNGLAR ALFFGKRGEL RERGIQDQLQ RASALNILIN AISVWNTVYL TEATKLLKEK GNLREDLLKH ISPLGWEHIN FLGEYNFDAR KVTSLHSLHP LLQ |
||||||||||
1. Matsui K, Yoshinami S, Narita M, Chien MF, Phung le T, Silver S, Endo G.
Mercury resistance transposons in Bacilli strains from different geographical regions.. FEMS Microbiol Lett. 2016 Mar;363(5):fnw013. doi: 10.1093/femsle/fnw013. Epub 2016 Jan 21. PubMed ID:
26802071
.