Mobile element type:
Transposon
Name:
Tn6133
Synonyms:
Accession:
Tn6133-FR772051.1
Family:
Tn554
Group:
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Staphylococcus aureus subsp. aureus ST398
Date of Isolation:
2011
Country:
Switzerland
Molecular Source:
chromosome
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| att (Tn6133) | Tn6133 | 1-6 | + | 6 | GATGTA |
| att (Tn6133) | Tn6133 | 11476-11481 | - | 6 | GATGTT |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpA | Tn6133 | 140-1225 | + | Transposase | |
| tnpB | Tn6133 | 1222-3114 | + | Transposase | |
| tnpC | Tn6133 | 3121-3498 | + | Accessory Gene | Helper |
| ANT(9)-Ia (ARO:3002630) | Tn6133 | 3649-4431 | + | Passenger Gene | Antibiotic Resistance |
| ErmA (ARO:3000347) | Tn6133 | 4557-5288 | - | Passenger Gene | Antibiotic Resistance |
| AXN76177.1 | Tn6133 | 5863-6360 | + | Passenger Gene | Hypothetical |
| WP_031795519.1 | Tn6133 | 6360-6776 | + | Passenger Gene | Hypothetical |
| WP_031795518.1 | Tn6133 | 6778-8346 | + | Passenger Gene | Hypothetical |
| vgaE (ARO:3002833) | Tn6133 | 8697-10271 | + | Passenger Gene | Antibiotic Resistance |
| SAM-dependent methyltransferase | Tn6133 | 10585-11247 | + | Passenger Gene | Other |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn6133 | 361 | 140-1225 | + |
|---|
| Class: | Transposase | ||||||||||
| Protein Sequence: | MKVQRIEVEN KPYPLYLLLD KEYQLIEPVM KFIKYLDNTG KSPNTIKAYC YHLKLLYEFM EQRGVILNDI NFELLADFVG WLRYPSASNV IDLQSKKAIR EETTVNTILN VVMSFLDYLS RLGEFKSIDV FKQAKGRNFK GFLHHVNKGR YQKNVLKLRV KKKQIRTLRS KEVKQIIDAC HTKRDKLILM LMYEGGLRIG EVLSLRLEDI VTWDNQIHLT PRDVNVNEAY IKLRKERTIH VSKELMSLYT DYLIYEYSEE LEHDYVFISL KEGYFGKPLK YQSVLDLVRR IVKRTGIEFT SHMLRHTHAT QLIREGWDVA FVQKRLGHAH VQTTLNTYVH LSDQDMKNEF NKYLERKEHK K |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpB | TnpB | Tn6133 | 630 | 1222-3114 | + |
|---|
| Class: | Transposase | ||||||||||
| Protein Sequence: | MNASSKRKII SQSEISKKIA VMNEEMQGFW ANNSWDIRKC PHPSAIELSK NPALRNRWVR FERVKNLWLR TELKYFYFYH LNNGIWNAKT VWIRKGTVIN KMLDFLDLKY PSITSITEVP IEKAMTEYRT YLTKRGVRIT TTNYKITANQ EKTPVKANSY YVTNLKQFME FYENFYFDGE EWDKDVWDRR NLPLPDDKVN PTQYEYTINF KGFRNTYFKQ LVKRYCKLRL NVDSFSYVSD IAQRLKEFFN FLDMKFKQVQ RVHQLTRVEI EAYLSELNMM GIKPSTITGR ISILEGLFST LLRLEWDDVP SKILIYSEDY PKIPRAKPRF IDEFVLEQLN SHLDKLPEYI ATMTMIVQEC GMRISELCTL KKGCLLEDKD GDFFLKYYQW KMKKEHIVPI SKEVALLIKV REDKVSEEFP DSEYLFPRKD GSPLKQETFR GELNKLAYEQ NIVDKSGEIY RFHAHAFRHT VGTRMINNGM PQHIVQKFLG HESPEMTSRY AHIFDETLKN EFTKFQEKLV TNNGDVLDLD EDNEVDDVEL QWFKKNINAQ VLPNGYCRLP VVAGGCPHAN ACLDCTHFCT SKQFLPQHEE QLERTEELLA IAKDKQWQRQ VETNSRVKER LEQIIGSLTG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpC | TnpC | Tn6133 | 125 | 3121-3498 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Helper | ||||||||||
| Sequence Family: | Tn554_family | ||||||||||
| Comment: | target specificity and orientation | ||||||||||
| Protein Sequence: | MDKQVRNTTE IVRLAKQKSK KTREKVDKAI SKFSIEGKVI NFNSIAKEAN VSKSWLYKEH DIRQRIESLR ERQITANVVS KPKKSSRSEE ILIKTLKRRV MELEKENKKL QNQIQKLYGD LYNKE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | ANT(9)-Ia (ARO:3002630) | ANT(9)-Ia | Tn6133 | 260 | 3649-4431 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | ANT(9) (ARO:3000228) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Protein Sequence: | MSNLINGKIP NQAIQTLKIV KDLFGSSIVG VYLFGSAVNG GLRINSDVDV LVVVNHSLPQ LTRKKLTERL MTISGKIGNT DSVRPLEVTV INRSEVVPWQ YPPKREFIYG EWLRGEFENG QIQEPSYDPD LAIVLAQARK NSISLFGPDS SSILVSVPLT DIRRAIKDSL PELIEGIKGD ERNVILTLAR MWQTVTTGEI TSKDVAAEWA IPLLPKEHVT LLDIARKGYR GECDDKWEGL YSKVKALVKY MKNSIETSLN |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | ErmA (ARO:3000347) | ErmA | Tn6133 | 243 | 4557-5288 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | Erm 23S ribosomal RNA methyltransferase (ARO:3000560) | ||||||||||
| Target: | streptogramin B antibiotic (ARO:0000050)||lincosamide antibiotic (ARO:0000017)||streptogramin A antibiotic (ARO:0000034)||macrolide antibiotic (ARO:0000000)||streptogramin antibiotic (ARO:0000026) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3000347||Synonyms: ermTR | ||||||||||
| Protein Sequence: | MNQKNPKDTQ NFITSKKHVK EILNHTNISK QDNVIEIGSG KGHFTKELVK MSRSVTAIEI DGGLCQVTKE AVNPSENIKV IQTDILKFSF PKHINYKIYG NIPYNISTDI VKRITFESQA KYSYLIVEKG FAKRLQNLQR ALGLLLMVEM DIKMLKKVPP LYFHPKPSVD SVLIVLERHQ PLISKKDYKK YRSFVYKWVN REYRVLFTKN QFRQALKHAN VTNINKLSKE QFLSIFNSYK LFH |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | AXN76177.1 | AXN76177.1 | Tn6133 | 165 | 5863-6360 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Comment: | ORF6133_1 | ||||||||||
| Protein Sequence: | MLFIKEERIH QAFITMMNKL KFGYSYVLIP LSKQLETSNQ DEDFQMITEI EKQIEVTKEE LNTLIQLMTK GFLEPAIFNQ QKIELSQRHM KLKEEREQLL YLINDGSNQL SEVKRIIKYF KQVNFIAAFD EETFQDIVRK VIVYSPNEIG FHLKCSITLK ERINR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | WP_031795519.1 | WP_031795519.1 | Tn6133 | 138 | 6360-6776 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Comment: | ORF6133_2 | ||||||||||
| Protein Sequence: | MAHTPFGYAM VNGKIEVQAK EAEQLKLLIA SYLSGQSLAN AARESGINRS HGGITRILTD ERYLGNGLFP RLISKEQFEQ LKNERFKRAK KLGRLNRTKE EESFTPCFEF TIGEVEDKYD NPIKQAEYVY SLIKEEVT |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | WP_031795518.1 | WP_031795518.1 | Tn6133 | 522 | 6778-8346 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Comment: | ORF6133_3| recombinase family protein [Staphylococcus aureus] | ||||||||||
| Protein Sequence: | MALARSVTVI PARRRTRNVD AEKKEKLKVA AYCRVSTDSE EQATSYEVQV EHYTTYIQNN PEWELAGIYA DDGISGTNTK KREQFNKMIE DSMNGKIDMI ITKSISRFAR NTLDCLKYIR QLKDKEIPVF FEKENINTMD AKGEIMLTIM ASLAQQESQS LSQNVKLGIQ YRYRQGEVQV NHNRFLGYTK DENNKLVINP EQAKIVKRIY REYLEGASLV QIARGLEADE ILTAAKRTKW RPESIKKILQ NEKYIGDALL QKTYTVDFLT KKRVVNNGHV PQYYVENNHE AIIPREIYLQ VQEEMMRRAN IRNGKTGKKR VYSSKYALSS IVFCKECEDV FRRVHWNNRG CRSIVWRCVS RLEGKTHLCN PETIREDELH HTIVQAINEV LGNKDDYLSI LLENIETVLN ENADKPTTDI DEKLEELQME LLRLANSKED YDDVAEEIYR LRELKQEVMM KNAAREGLRQ RIEEMTKFLK EQPQKLETYD DLLVRRLIEK ITIHERLLTI VFKSGIEVKK EI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vgaE (ARO:3002833) | VgaE | Tn6133 | 524 | 8697-10271 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target protection (ARO:0001003) | ||||||||||
| Sequence Family: | vga-typeABC-F protein (ARO:3000113) | ||||||||||
| Target: | streptogramin antibiotic (ARO:0000026)||pleuromutilin antibiotic (ARO:3000670)||streptogramin A antibiotic (ARO:0000034) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002833 | ||||||||||
| Protein Sequence: | MLLFEGTSLK KHIQDRLLFD IDLIQVHEHQ RIGLVGRNGT GKTSLLKIIT GEELVDGGNV NHFTSVKLVP QFKETRSEKS GGEITQQYLQ LAFNEKPGLL ILDEPTTHLD TQRIDWLEKK LANYQGAFVV VSHDRTFLNN VCTEIWEIED GSLNAFKGDY NAYAEQKELI KTQQQIAFEK YEREKKQLEK AIRQKEERAQ RATKKPKNLS SSEARITGAK THYANIQKKL RGSAKALETR LEQLDRIDKV KELPEIKMDI LNKESLTNQS VLRAENIKGE VDGRKLWNPF SLYLYGGDKV AIIGKNGTGK TTLLKKIVER DERIAIPEKV RIGYFSQHLT ILDDDKTIIE NIQLTSSQDE TLIRTVLARM HFWDEDVYKK VGILSGGEKV KVALAKLFLS DVNMLVLDEP TNFLDIESLE ALETLMKSYH GTILFVTHDR TLVTNIATKI IDIKDGKITV FDGSYEAYEE WLENQTKSNN DDQLLLIETK ISDVLGRLSL EPSRELEDEF QRLLKEKKEL TKKL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | SAM-dependent methyltransferase | SAM-dependent methyltransferase | Tn6133 | 220 | 10585-11247 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | perfect match to reference sequence for Uniprot A0A347WKB4 and Q5HNP8||Synonyms: | ||||||||||
| Protein Sequence: | MNTIESQFDK VAEDYDFVNE LLNDYSFFVS NMSPKKGRAL DIGCGSGLLV EKLASYYDEV VGIDISNQML DLAKSKRQLT NTVYLNMNAE QLNFNEKFDF IVSRTTFHHL DDIASVIQQM KELLNEEGRI VILDNVSEVE TPPTYVYKLG AIQEFLPHCF KFGIKNAIRI YNHNTSKSWL EHLASDKYLS EQNYYDLYEK LLPGCQFHKM GWAMGVVWTK |
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1. Schwendener S, Perreten V.
New transposon Tn6133 in methicillin-resistant Staphylococcus aureus ST398 contains vga(E), a novel streptogramin A, pleuromutilin, and lincosamide resistance gene.. Antimicrob Agents Chemother. 2011 Oct;55(10):4900-4. doi: 10.1128/AAC.00528-11. Epub 2011 Jul 18. PubMed ID:
21768510
.