Mobile element type:
Transposon
Name:
Tn6023
Synonyms:
Accession:
Tn6023-GU562437.2
Family:
Compound Transposon
Group:
IS26
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Salmonella enterica subsp. enterica serovar Typhimurium
Date of Isolation:
2000
Country:
Usa
Molecular Source:
plasmid pSRC125
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpA | IS26 | 53-757 | - | Transposase | |
| APH(3')-Ia (ARO:3002641) | Tn6023 | 908-1723 | + | Passenger Gene | Antibiotic Resistance |
| tnpA | IS26.var1 | 1880-2584 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | IS26 | 234 | 53-757 | - |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MNPFKGRHFQ RDIILWAVRW YCKYGISYRE LQEMLAERGV NVDHSTIYRW VQRYAPEMEK RLRWYWRNPS DLCPWHMDET YVKVNGRWAY LYRAVDSRGR TVDFYLSSRR NSKAAYRFLG KILNNVKKWQ IPRFINTDKA PAYGRALALL KREGRCPSDV EHRQIKYRNN VIECDHGKLK RIIGATLGFK SMKTAYATIK GIEVMRALRK GQASAFYYGD PLGEMRLVSR VFEM |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | APH(3')-Ia (ARO:3002641) | APH(3')-Ia | Tn6023 | 271 | 908-1723 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | APH(3') (ARO:3000126) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002641||Synonyms: aphA-1, apha1-1AB, APH(3')-Ic, apha7 | ||||||||||
| Protein Sequence: | MSHIQRETSC SRPRLNSNLD ADLYGYRWAR DNVGQSGATI YRLYGKPNAP ELFLKHGKGS VANDVTDEMV RLNWLTAFMP LPTIKHFIRT PDDAWLLTTA IPGKTAFQVL EEYPDSGENI VDALAVFLRR LHSIPVCNCP FNSDRVFRLA QAQSRMNNGL VDASDFDDER NGWPVEQVWK EMHKLLPFSP DSVVTHGDFS LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS PSLQKRLFQK YGIDNPDMNK LQFHLMLDEF F |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | IS26.var1 | 234 | 1880-2584 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | Has one amino acid change relative to tnpA (IS26) | ||||||||||
| Protein Sequence: | MNPFKGRHFQ RDIILWAVRW YCKYGISYRE LQEMLAERGV NVDHSTIYRW VQRYAPEMEK RLRWYWRNPS DLCPWHMDET YVKVNGRWAY LYRAVDSRGR TVDFYLSSRR NSKAAYRFLG KILNNVKKWQ IPRFINTDKA PAYGRALALL KREGRCPSDV EHRQIKYRNN VIECDHGKLK RIINATLGFK SMKTAYATIK GIEVMRALRK GQASAFYYGD PLGEMRLVSR VFEM |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| IS26-X00011 | IS26 | insertion sequence | 1-820 | + | 820 |
| IS26.var1-GU562437.2 | IS26.var1 | insertion sequence | 1817-2636 | + | 820 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRR | IS26 | 1-14 | + | 14 | GGCACTGTTG CAAA |
| IRL | IS26 | 807-820 | - | 14 | GGCACTGTTG CAAA |
| IRL | IS26.var1 | 1817-1830 | + | 14 | GGCACTGTTG CAAA |
| IRR | IS26.var1 | 2623-2636 | - | 14 | GGCACTGTTG CAAA |
1. Cain AK, Hall RM.
Transposon Tn5393e carrying the aphA1-containing transposon Tn6023 upstream of strAB does not confer resistance to streptomycin.. Microb Drug Resist. 2011 Sep;17(3):389-94. doi: 10.1089/mdr.2011.0037. Epub 2011 Jun 24. PubMed ID:
21702681
.