Mobile element type:
Transposon
Name:
Tn5718
Synonyms:
Accession:
Tn5718-AJ304453
Family:
Tn402
Group:
Tn5053
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Uncultured bacterium plasmid pSB102
Date of Isolation:
2001
Country:
Germany
Molecular Source:
plasmid pSB102
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRt | 1-25 | + | 25 | TGTCATTTTC AGAAGACGAC TGCAC |
| repeat t1 | 9-27 | + | 19 | TCAGAAGACG ACTGCACCA |
| repeat t2 | 49-67 | - | 19 | TCAGGAGTCG CCTGCACAT |
| repeat t4 | 110-128 | + | 19 | TCAATACTCG TGTGCACCA |
| repeat t4 | 10324-10342 | - | 19 | TCAGAAAACG GAAAATAAA |
| IRi | 10390-10414 | - | 25 | TGTCATTTTC AGAAGGTGAC TGCAC |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| repeat t3 | Tn5718 | 78-97 | + | 20 | TCAGAAGTCA TCTGCACCAA |
| r5 | Tn5718 | 3872-3885 | + | 14 | AATTTCTGTC ACAT |
| r3 | Tn5718 | 3928-3941 | + | 14 | GGGTTAAGTG ACAA |
| res | Tn5718 | 3972-4001 | + | 30 | CTGTCACATA ATCGAACGTA TATGTGACAG |
| r2 | Tn5718 | 3972-3985 | - | 14 | CGATTATGTG ACAG |
| r1 | Tn5718 | 3988-4001 | + | 14 | CGTATATGTG ACAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tniA | Tn5718 | 144-1823 | + | Transposase | |
| tniB | Tn5718 | 1826-2734 | + | Accessory Gene | |
| tniQ | Tn5718 | 2731-3948 | + | Accessory Gene | Target Site Selection |
| tniR | Tn5718 | 4009-4623 | + | Accessory Gene | Resolvase |
| tniM | Tn5718 | 4662-4982 | - | Passenger Gene | Hypothetical |
| merE | Tn5718 | 4998-5234 | - | Passenger Gene | Heavy Metal Resistance |
| merD | Tn5718 | 5231-5692 | - | Passenger Gene | Heavy Metal Resistance |
| merB | Tn5718 | 5708-6346 | - | Passenger Gene | Heavy Metal Resistance |
| merA | Tn5718 | 6361-8046 | - | Passenger Gene | Heavy Metal Resistance |
| merC | Tn5718 | 8085-8507 | - | Passenger Gene | Heavy Metal Resistance |
| merG | Tn5718 | 8516-9154 | - | Passenger Gene | Heavy Metal Resistance |
| merP | Tn5718 | 9171-9446 | - | Passenger Gene | Heavy Metal Resistance |
| merT | Tn5718 | 9462-9812 | - | Passenger Gene | Heavy Metal Resistance |
| merR | Tn5718 | 9884-10318 | + | Passenger Gene | Heavy Metal Resistance |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniA | TniA | Tn5718 | 559 | 144-1823 | + |
|---|
| Class: | Transposase | ||||||||||
| Function: | putative transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MASDTSLIAE QGVATLPDAA WAQARQRAEI IGPLAALDVV GHEAADAAAH ALGLSRRQVY VLIRRARQGA GLVTDLARSR SGGGKGKGRL PESVERIIRE LLQKRFLTKQ KRSLAAFHRE VAQACKAQKL RAPARNTVAL RIAGLDPLKA TRRREGQDAS RSLQGVGGEP PAVTAPLEQV QIDHTVIDLI VVDERDRQPI GRPYLTIAID VFTRCVLGMV VTLEAPSSVS VGLCLVHVAC DKRPWLEGLN IEMEWPMSGK PRLLYLDNAA EFKSEALRRG CEQHGIRLDY RPLGQPHYGG IVERIIGTAM QMIHDELPGT TFSNPDQRGD YDSENKAALT LRELERWLTL AVGTYHGSVH NGLLQPPAAR WSEAVARVGV PAVVTRALAF LVDFLPIIRR TLTRTGFVID HIHYYADALK PWIARRDRLP AFLIRRDPRD ISRIWVLEPE GQHYLEIPYR TLSHPAVTLW EQRQALTKLR QQGREQVDES ALFRMIGQMR EIVTTAQKAT RKARRDADRR QHLKASPPPD KPIPPKTDVA DPQADNLPPA KPFDQIEEW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniB | TniB | Tn5718 | 302 | 1826-2734 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Sequence Family: | ATP binding protein? | ||||||||||
| Protein Sequence: | MDEYPIIDLS HLLPAAQGLA RLPADERIQR LRADRWIGYP RAVEALNRLE TLYAWPNKQR MPNLLLVGPT NNGKSMIIEK FRRTHPASSD ADQEHMPVLV VQMPSEPSVI RFYVALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRD AYLAIRSDDQ LENRFEPMML PVWEANDDCC SLLASFAASL PLRRPSSIAT LDMARYLLTR SEGTIGELAH LLMAAALVAV ESGEEAINHR TLSMADYTGP SERRRQFERE LM |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniQ | TniQ | Tn5718 | 405 | 2731-3948 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Target Site Selection | ||||||||||
| Function: | putative transposition gene | ||||||||||
| Comment: | similar function to Tn7 tnsD? | ||||||||||
| Protein Sequence: | MKPAPRWPLH PAPREAEALS SWLNRVALCY HMEVSELLEH DLGHGQVDDL DTAPPLALLA MLSQRSGIEL DRLRCMSFAG WVPWLLDSLD DQIPAALETY AFQLSVLLPR LRRKTRSITS WRAWLPTQPI HRACPLCLND PENQAVLLAW KLPLMLSCPL HGCWLESYWG VPGRFLGWEN ADAEPRTASD AIAAMDQRTW QALTTGHVEL PRRRIHAGLW FRLLRTLLDE LNTPLSTCGT YAGYLRQIWE CCGHPLRAGQ SLWRPYETLN PAVRLQMLEA AATAISLIEV RDISPPGEHA KLFWSEPQTG FTSGLPAKAP KPEPVDHWQR AIQAIDEAII EARHNPETAR SLFALASYGR RDPASLEQLR ATFAKEGIPP EFLSHYEPSL PFACLRQNDG LSDKF |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniR | TniR | Tn5718 | 204 | 4009-4623 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | putative resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | resolution of cointegrates || Protein: ACE81792.1 | ||||||||||
| Protein Sequence: | MLIGYMRVSK ADGSQATDLQ RDALVAAGVD PAHLYEDQAS GKREDRPGLA SCLKALRPGD TLVVWKLDRL GRDLRHLINT VHDLTGRGIG LKVLTGHGAA IDTTTAAGKL VFGIFAALAE FERELIAERT VAGLASARAR GRKGGRPFKM TAAKLRLAMA AMGQSETKVG DLCQELGITR QTLYRHISPK GELRPDGEKL LSRI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniM | TniM | Tn5718 | 106 | 4662-4982 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Function: | putative modulator protein of transposition | ||||||||||
| Protein Sequence: | MDGLRRRAQA FERGQGFLFA KPMPAAAFAV FVSQWRGATM NANDPTATSC CVCCKEIPLD AAFTPEGAEY VEHFCGLECY QRFQARASTA TETSGEPNAC GSPPSN |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merE | MerE | Tn5718 | 78 | 4998-5234 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercury transport | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | similar to urf-1 in pKLH2 (GenBank AF213017), pKLH272 (Genbank Y08992), pMER610 (GenBank Y08993), pKLH210 (GenBank Y10102), Tn5036 (Genbank Y09025), orf1 in Tn501 (GenBank Z00027), and urf-1 in Tn5041 (GenBank X98999) | ||||||||||
| Protein Sequence: | MNSPERMPAE THKPFTGYLW GTLAVLTCPC HLSILAAVLA GTTAGAFLVE YWVITALGLT GLFLLSLARA LRAFRERS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD | MerD | Tn5718 | 153 | 5231-5692 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MFYGTGIGFA ARGLNSAKRY MHCREPTKRR CSMNAYTVSR LALDAGVSVH IVRDYLLRGL LRPVACTTGG YGLFDDAALQ RLCFVRAAFE AGIGLGALAR LCRALDAANC DETAAQLAVL RQFVERRREA LANLEVQLAA MPTAPAQHAE SLP |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merB | MerB | Tn5718 | 212 | 5708-6346 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | putative organomercurial lyase | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | organomercurial lyase | ||||||||||
| Protein Sequence: | MKLAPYILER LTSVNRTNGT ADLLVPLLRE LAKGRPVSRT TLAGILDWPA ERVAAVLEQA TSTEYDKDGN IIGYGLTLRE TSYVFEIDDR RLYAWCALDT LIFPALIGRT ARVSSHCAAT GAPVSLTVSP SEIQAVEPAG MAVSLVLPQE AADVRQSFCC HVHFFASVPT AEDWASKHQG LEGLAIVSVH EAFGLGQEFN RHLLQTMSSR TP |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA | MerA | Tn5718 | 561 | 6361-8046 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | putative mercuric reductase | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MTTLKITGMT CDSCATHVKE ALEKVPGVQS AVVSYAKGAA QLDLDPGTAS DALTAAVAGL GYKATFADTL STDNRAGLLD KVRGWMGTAD KGSDGERQLQ VAVIGSGGAA MAAALKAVEQ GAKVTLIERG TIGGTCVNIG CVPSKIMIRA AHIAHLRRES PFDDGIAATV PAINRSKLLA QQQARVDELR HAKYEGILDG NPAITVLHGE ARFKDDQSLT VRLNDGGERV VVFDRCLVAT GASPAIPPIT GLKGTPYWTS TEALVSDTIP ERLAVIGSSV VALELAQAFA RLGSQVTILA RSTLFFREDP AIGEAVTAAF RAEGIKVLEH TQASQVAYAD GEFVLTTGHG EVRADKLLVA TGRTPNTRSL ALEAAGVAVN AQGAIVIDKG MRTSTPHIYA AGDCTDQPQF VYVAAAAGTR AAINMTGGDA TLNLTAMPAV VFTDPQVATV GYSEAEAHHD GIETDSRLLT LDNVPRALAN FDTRGFIKLV IEEGSGRLIG VQAVAPEAGE LIQTAVLAIR NRMTVQELAD QLFPYLTMVE GLKLAAQTFT KDVKQLSCCA G |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merC | MerC | Tn5718 | 140 | 8085-8507 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | putative mercury transport protein | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MGLITRIADK TGALGSVVSA MGCAACFPAI ASFGAAIGLG FLSEYEGLFI SRLLPLFAAI ALLANALGWL SHRQWHRSLL GMIRPAIVFA ATVWLLGNWW TANLMYVGLA LMVGVSIWDF VSPAHRRCGP DGCELPPQHG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merG | MerG | Tn5718 | 212 | 8516-9154 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | putative phenylmercury resistance protein | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MMSNLFRYGR SSVVSGLLML MSVGALAYDF KRMEAAIESL SPTTQSEWER KATAGEPIAQ NVTGMAYKYG IGVAQDQAVS WKWFRSAAEQ GEADAQFNLA RIYEGHANDT FYRRHARPVP ADDSEAVNWY RRSAEQNHTQ AQVKLAQLYA KGVAPAASDL VQAYKWFGIA AARGDETAVK LRAELAARLT REQIADGDAQ AMEWTRSSSV KQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | Tn5718 | 91 | 9171-9446 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | periplasmic mercuric ion binding protein | ||||||||||
| Protein Sequence: | MKKLFAALAL AAVVAPVWAA TQTVTLSVPG MTCSTCPIIV KKAISKVEGV SKIDVTFETR EAVVTFDDAK TSVQKLIKAT GDAGFPSSVK Q |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | Tn5718 | 116 | 9462-9812 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercuric ion transport protein | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MSEPQTGRGA LFTGGLAAIL ASACCLGPLV LVALGFSGAW IGNLTVLEPY RPIFIGAALV ALFFAWRRIY RPTQACKPGE VCAIPQVRAT YKLIFWVVAA LVLVSLGFPY VMPFFY |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR | MerR | Tn5718 | 144 | 9884-10318 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | putative regulatory gene mercury resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MENNLENLTI GVFAKAAGVN VETIRFYQRK GLLPEPDKPY GSIRRYGAAD VTRVRFVKSA QRLGFSLDEI AELLRLDDGT HCEEASSLAE HKLQDVREKM ADLARMEAVL SDLVCACHSR QGNVSCPLIA SLQGGTSLAG ASTA |
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1. Schneiker S, Keller M, Droge M, Lanka E, Puhler A, Selbitschka W.
The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa.. Nucleic Acids Res. 2001 Dec 15;29(24):5169-81. doi: 10.1093/nar/29.24.5169. PubMed ID:
11812851
.