Mobile element type:
Transposon
Name:
Tn554
Synonyms:
Accession:
Tn554-X03216.1.1
Family:
Tn554
Group:
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Staphylococcus aureus
Date of Isolation:
1979
Country:
Usa
Molecular Source:
genome
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| att (Tn554) | Tn554 | 6686-6691 | + | 6 | GATGTA |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpA | Tn554 | 134-1219 | + | Transposase | |
| tnpB | Tn554 | 1216-3108 | + | Transposase | |
| tnpC | Tn554 | 3115-3492 | + | Accessory Gene | Helper |
| ANT(9)-Ia (ARO:3002630) | Tn554 | 3643-4425 | + | Passenger Gene | Antibiotic Resistance |
| ErmA (ARO:3000347) | Tn554 | 4551-5282 | - | Passenger Gene | Antibiotic Resistance |
| SAM-dependent methyltransferase | Tn554 | 5795-6457 | + | Passenger Gene | Other |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn554 | 361 | 134-1219 | + |
|---|
| Class: | Transposase | ||||||||||
| Protein Sequence: | MKVQRIEVEN KPYPLYLLLD KEYQLIEPVM KFIKYLDNTG KSPNTIKAYC YHLKLLYEFM EQRGVILNDI NFELLADFVG WLRYPSASNV IDLQSKKAIR EETTVNTILN VVMSFLDYLS RLGEFKSIDV FKQAKGRNFK GFLHHVNKGR YQKNVLKLRV KKKQIRTLRS KEVKQIIDAC HTKRDKLILM LMYEGGLRIG EVLSLRLEDI VTWDNQIHLT PRDVNVNEAY IKLRKERTIH VSKELMSLYT DYLIYEYSEE LEHDYVFISL KEGYFGKPLK YQSVLDLVRR IVKRTGIEFT SHMLRHTHAT QLIREGWDVA FVQKRLGHAH VQTTLNTYVH LSDQDMKNEF NKYLERKEHK K |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpB | TnpB | Tn554 | 630 | 1216-3108 | + |
|---|
| Class: | Transposase | ||||||||||
| Protein Sequence: | MNASSKRKII SQSEISKKIA VMNEEMQGFW ANNSWDIRKC PHPSAIELSK NPALRNRWVR FERVKNLWLR TELKYFYFYH LNNGIWNAKT VWIRKGTVIN KMLDFLDLKY PSITSITEVP IEKAMTEYRT YLTKRGVRIT TTNYKITANQ EKTPVKANSY YVTNLKQFME FYENFYFDGE EWDKDVWDRR NLPLPDDKVN PTQYEYTINF KGFRNTYFKQ LVKRYCKLRL NVDSFSYVSD IAQRLKEFFN FLDMKFKQVQ RVHQLTRVEI EAYLSELNMM GIKPSTITGR ISILEGLFST LLRLEWDDVP SKILIYSEDY PKIPRAKPRF IDEFVLEQLN SHLDKLPEYI ATMTMIVQEC GMRISELCTL KKGCLLEDKD GDFFLKYYQW KMKKEHIVPI SKEVALLIKV REDKVSEEFP DSEYLFPRKD GSPLKQETFR GELNKLAYEQ NIVDKSGEIY RFHAHAFRHT VGTRMINNGM PQHIVQKFLG HESPEMTSRY AHIFDETLKN EFTKFQEKLV TNNGDVLDLD EDNEVDDVEL QWFKKNINAQ VLPNGYCRLP VVAGGCPHAN ACLDCTHFCT SKQFLPQHEE QLERTEELLA IAKDKQWQRQ VETNSRVKER LEQIIGSLTG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpC | TnpC | Tn554 | 125 | 3115-3492 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Helper | ||||||||||
| Sequence Family: | Tn554_family | ||||||||||
| Comment: | target specificity and orientation | ||||||||||
| Protein Sequence: | MDKQVRNTTE IVRLAKQKSK KTREKVDKAI SKFSIEGKVI NFNSIAKEAN VSKSWLYKEH DIRQRIESLR ERQITANVVS KPKKSSRSEE ILIKTLKRRV MELEKENKKL QNQIQKLYGD LYNKE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | ANT(9)-Ia (ARO:3002630) | ANT(9)-Ia | Tn554 | 260 | 3643-4425 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | ANT(9) (ARO:3000228) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Protein Sequence: | MSNLINGKIP NQAIQTLKIV KDLFGSSIVG VYLFGSAVNG GLRINSDVDV LVVVNHSLPQ LTRKKLTERL MTISGKIGNT DSVRPLEVTV INRSEVVPWQ YPPKREFIYG EWLRGEFENG QIQEPSYDPD LAIVLAQARK NSISLFGPDS SSILVSVPLT DIRRAIKDSL PELIEGIKGD ERNVILTLAR MWQTVTTGEI TSKDVAAEWA IPLLPKEHVT LLDIARKGYR GECDDKWEGL YSKVKALVKY MKNSIETSLN |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | ErmA (ARO:3000347) | ErmA | Tn554 | 243 | 4551-5282 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | Erm 23S ribosomal RNA methyltransferase (ARO:3000560) | ||||||||||
| Target: | streptogramin B antibiotic (ARO:0000050)||lincosamide antibiotic (ARO:0000017)||streptogramin A antibiotic (ARO:0000034)||macrolide antibiotic (ARO:0000000)||streptogramin antibiotic (ARO:0000026) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3000347||Synonyms: ermTR | ||||||||||
| Protein Sequence: | MNQKNPKDTQ NFITSKKHVK EILNHTNISK QDNVIEIGSG KGHFTKELVK MSRSVTAIEI DGGLCQVTKE AVNPSENIKV IQTDILKFSF PKHINYKIYG NIPYNISTDI VKRITFESQA KYSYLIVEKG FAKRLQNLQR ALGLLLMVEM DIKMLKKVPP LYFHPKPSVD SVLIVLERHQ PLISKKDYKK YRSFVYKWVN REYRVLFTKN QFRQALKHAN VTNINKLSKE QFLSIFNSYK LFH |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | SAM-dependent methyltransferase | SAM-dependent methyltransferase | Tn554 | 220 | 5795-6457 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | perfect match to reference sequence for Uniprot A0A347WKB4 and Q5HNP8||Synonyms: | ||||||||||
| Protein Sequence: | MNTIESQFDK VAEDYDFVNE LLNDYSFFVS NMSPKKGRAL DIGCGSGLLV EKLASYYDEV VGIDISNQML DLAKSKRQLT NTVYLNMNAE QLNFNEKFDF IVSRTTFHHL DDIASVIQQM KELLNEEGRI VILDNVSEVE TPPTYVYKLG AIQEFLPHCF KFGIKNAIRI YNHNTSKSWL EHLASDKYLS EQNYYDLYEK LLPGCQFHKM GWAMGVVWTK |
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1. Murphy E, Huwyler L, de Freire Bastos Mdo C.
Transposon Tn554: complete nucleotide sequence and isolation of transposition-defective and antibiotic-sensitive mutants.. EMBO J. 1985 Dec 1;4(12):3357-65. doi: 10.1002/j.1460-2075.1985.tb04089.x. PubMed ID:
3004956
.