Mobile element type:
Transposon
Name:
Tn551
Synonyms:
Tn917
Accession:
Tn551-Y13600.1
Family:
Tn3
Group:
Tn4330
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Staphylococcus aureus
Date of Isolation:
1977
Country:
Molecular Source:
plasmid pI258 (Tn551) and pAD2 (Tn917)
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGTCCCGA GCGCCTACGA GAAATTTGTA TCGATAAG |
| IRR | 5229-5266 | - | 38 | GGGGTCCCGA GCGCTTAGTG GGAATTTGTA TCGATAAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| ErmB (ARO:3000375) | Tn551 | 625-1362 | + | Passenger Gene | Antibiotic Resistance |
| tnpR | Tn551 | 1717-2271 | + | Accessory Gene | Resolvase |
| tnpA | Tn551 | 2275-5193 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | ErmB (ARO:3000375) | ErmB | Tn551 | 245 | 625-1362 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | Erm 23S ribosomal RNA methyltransferase (ARO:3000560) | ||||||||||
| Target: | macrolide antibiotic (ARO:0000000)||lincosamide antibiotic (ARO:0000017)||streptogramin antibiotic (ARO:0000026) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3000375 (97% identity, bitscore: 489) | ||||||||||
| Protein Sequence: | MNKNIKYSQN FLTSEKVLNQ IIKQLNLKET DTVYEIGTGK GHLTTKLAKI SKQVTSIELD SHLFNLSSEK LKLNIRVTLI HQDILQFQFP NKQRYKIVGN IPYHLSTQII KKVVFESHAS DIYLIVEEGF YKRTLDIHRT LGLLLHTQVS IQQLLKLPAE CFHPKPKVNS VLIKLTRHTT DVPDKYWKLY TYFVSKWVNR EYRQLFTKNQ FHQAMKHAKV NNLSTVTYEQ VLSIFNSYLL FNGRK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn551 | 184 | 1717-2271 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | resolvase||serine site-specific recombinase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | identical to tnpR (Tn917) | ||||||||||
| Protein Sequence: | MIFGYARVST DDQNLSLQID ALTHYGIDKL FQEKVTGAKK DRPQLEEMIN LLREGDSVVI YKLDRISRST KHLIELSELF EELSVNFISI QDNVDTSTSM GRFFFRVMAS LAELERDIII ERTNSGLKAA RVRGKKGGRP SKGKLSIDLA LKMYDSKEYS IRQILDASKL SKTTFYRYLN KRNA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn551 | 972 | 2275-5193 | + |
|---|
| Class: | Transposase | ||||||||||
| Function: | transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | identical totnpA (Tn917) | ||||||||||
| Protein Sequence: | MAMKRILTTS QREQLLSVDH LSEEDFKAYF SFSDYDLEVI NQHRGKVNKL GFAIQLCLAR YPGCSLSNWP IKSTRLTSYV SRQLHLDAID LNSYDHRNTR ANHFNEILEV FNYHRFGSAN TQKQLIEYLI ELALENDDSI YLMKKTIDFL TRKRIIFPSI ATLEDIISRC RDKAENNLFS ILLCSLTDIQ IEKLESLFQI YEETKITKLA WLKDIPGKAN PESFMSICKK VEVIASMGLG TINVSHINRN RFLQLARLGE NYDAYDFSRF ELEKRYSLLI AFLVNHHQYL IDQLIEINDR ILASIKRKGT RDSQEQLKEK GKLATKKLEH YASLIDALHF AKDNDSNPFD EIERIMPWED LVQDGEEAKK ITGNKNHGYL EMVRNKANYL RRYTPMLLRT LSFKATPAAN PVLMALTQLT DLHNSGKRKI PADTSTDFVS KKWKSLVRPE EGKIDRSYYE LVAFTELKNN IRSGDISVEG SMIHRNIDDY LVDLSACIDS ETIPDTFEDY LKDREIILDL QLQFYSTVDK RISRANLKKL EKVTPSEAEI YRKKLYSIIP KIRLSDLLIE VDSWTNFSQE FSHDSTGKPP SEQERKIIFA ALLGLGMNIG LEKMAQSTPG ISYSQLANAK QWRFYKEALT RAQSVLVNYQ LKLPVADFWG EGKTTASDGM RVPVGVSALK SDVNPHYKSM EKGATMIRSI NDRHTTHHIE VASTNTREAT HTLDGLLYHE TDLDIEEHFT DTNGYSDQVF GMTALLGFDF EPRIRNIKKS QLFSIKSPSY YPNLSEDISG KINVKIIEEN YDEIKRIAYS IQTAKVSSSL LLGKLGSYAR KNRVALALRE LGRIEKSIFM IDYITDSELR RRITHGLNKT EAINALAREL FFGRRGKFME RDIRRQLQSA SALNVLINAI SIWNAVYLQA AYNYLVKIDP EVTKYMKHVS PINWEHITFL GEYKFDLLSI PKHLRELNIK NK |
||||||||||
1. Pattee PA, Thompson NE, Haubrich D, Novick RP.
Chromosomal map locations of integrated plasmids and related elements in Staphylococcus aureus.. Plasmid. 1977 Nov;1(1):38-51. doi: 10.1016/0147-619x(77)90007-5. PubMed ID:
618184
.
2. Tomich PK, An FY, Clewell DB. A transposon (Tn917) in Streptococcus faecalis that exhibits enhanced transposition during induction of drug resistance.. Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1217-21. doi: 10.1101/sqb.1979.043.01.138. PubMed ID: 114353 .
3. Tomich PK, An FY, Clewell DB. Properties of erythromycin-inducible transposon Tn917 in Streptococcus faecalis.. J Bacteriol. 1980 Mar;141(3):1366-74. doi: 10.1128/jb.141.3.1366-1374.1980. PubMed ID: 6245068 .
4. Khan SA, Novick RP. Terminal nucleotide sequences of Tn551, a transposon specifying erythromycin resistance in Staphylococcus aureus: homology with Tn3.. Plasmid. 1980 Sep;4(2):148-54. doi: 10.1016/0147-619x(80)90004-9. PubMed ID: 6100928 .
5. Perkins JB, Youngman PJ. A physical and functional analysis of Tn917, a Streptococcus transposon in the Tn3 family that functions in Bacillus.. Plasmid. 1984 Sep;12(2):119-38. doi: 10.1016/0147-619x(84)90058-1. PubMed ID: 6095351 .
6. Wu SW, de Lencastre H, Tomasz A. The Staphylococcus aureus transposon Tn551: complete nucleotide sequence and transcriptional analysis of the expression of the erythromycin resistance gene.. Microb Drug Resist. 1999 Spring;5(1):1-7. doi: 10.1089/mdr.1999.5.1. PubMed ID: 10332716 .
2. Tomich PK, An FY, Clewell DB. A transposon (Tn917) in Streptococcus faecalis that exhibits enhanced transposition during induction of drug resistance.. Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1217-21. doi: 10.1101/sqb.1979.043.01.138. PubMed ID: 114353 .
3. Tomich PK, An FY, Clewell DB. Properties of erythromycin-inducible transposon Tn917 in Streptococcus faecalis.. J Bacteriol. 1980 Mar;141(3):1366-74. doi: 10.1128/jb.141.3.1366-1374.1980. PubMed ID: 6245068 .
4. Khan SA, Novick RP. Terminal nucleotide sequences of Tn551, a transposon specifying erythromycin resistance in Staphylococcus aureus: homology with Tn3.. Plasmid. 1980 Sep;4(2):148-54. doi: 10.1016/0147-619x(80)90004-9. PubMed ID: 6100928 .
5. Perkins JB, Youngman PJ. A physical and functional analysis of Tn917, a Streptococcus transposon in the Tn3 family that functions in Bacillus.. Plasmid. 1984 Sep;12(2):119-38. doi: 10.1016/0147-619x(84)90058-1. PubMed ID: 6095351 .
6. Wu SW, de Lencastre H, Tomasz A. The Staphylococcus aureus transposon Tn551: complete nucleotide sequence and transcriptional analysis of the expression of the erythromycin resistance gene.. Microb Drug Resist. 1999 Spring;5(1):1-7. doi: 10.1089/mdr.1999.5.1. PubMed ID: 10332716 .