Mobile element type:
Transposon
Name:
Tn5501.6
Synonyms:
Accession:
Tn5501.6-MF487840.1
Family:
Tn3
Group:
Tn3000
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Pseudomonas aeruginosa strain PA34
Date of Isolation:
2017
Country:
India
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGTTCTAA GCCGGAACCG CCGAAAATTC CGTCAGCC |
| IRR | 5692-5729 | - | 38 | GGGGTTCTAA GCCGGAACCG CCGAAATTTC CGTCATCC |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | Tn5501.6 | 1940-2070 | + | 131 | GCCTGTCGGA AAACATTTGT TTTGCGACAG GCCTTCAACA GTCCTCTGCA CCAACCTCCG AGTGGCCGCA AAATTGTGCG GAAAACTCTG TCGCCAGACG CTACCATGCG GAAACCCCTT CTTGATGGTT T |
| res_site_I | Tn5501.6 | 1940-1968 | + | 29 | GCCTGTCGGA AAACATTTGT TTTGCGACA |
| res_site_II | Tn5501.6 | 2002-2045 | + | 44 | TGGCCGCAAA ATTGTGCGGA AAACTCTGTC GCCAGACGCT ACCA |
| res_site_III | Tn5501.6 | 2046-2070 | + | 25 | TGCGGAAACC CCTTCTTGAT GGTTT |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| NPS-1 (ARO:3004239) | Tn5501.6 | 343-1125 | - | Passenger Gene | Antibiotic Resistance |
| parE | Tn5501.6 | 1280-1564 | - | Passenger Gene | Toxin |
| parD | Tn5501.6 | 1561-1839 | - | Passenger Gene | Antitoxin |
| tnpR | Tn5501.6 | 2093-2671 | + | Accessory Gene | Resolvase |
| tnpA | Tn5501.6 | 2668-5697 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | NPS-1 (ARO:3004239) | NPS-1 | Tn5501.6 | 260 | 343-1125 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | NPS beta-lactamase (ARO:3004240) | ||||||||||
| Target: | cephalosporin (ARO:0000032)||penam (ARO:3000008) | ||||||||||
| Comment: | 100% match to reference sequence ARO:3004239 (bit score: 538) | ||||||||||
| Protein Sequence: | MLKSTLLAFG LFIALSARAE NQAIAQLFQR AGVDGTIVIE SLTTRQRLVH NDPRAQQRYP AASTFKVLNT LIALEEGAIS GENQIFHWNG TQYSIANWNQ DQTLDSAFKV SCVWCYQQIA LRVGALKYPA YIQQTNYGHL LEPFNGTEFW LDGSLTISAE EQVAFLRRVV ERKLPFKASS YDSLKKVMFA DENAQYRLYA KTGWATRITP SVGWYVGYVE AQDDVWLFAL NLATRDANDL PLRTQIAKDA LKAIGAFHAK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | parE | ParE | Tn5501.6 | 94 | 1280-1564 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Toxin | ||||||||||
| Sequence Family: | ParE_toxin (Pfam:PF05016) | ||||||||||
| Target: | DNA gyrase | ||||||||||
| Protein Sequence: | VRVVWTPEAQ QDRADVWDYI AADNPRAAAR MDEIFSDAAA RLIQHPMLGK PGKIPGTREL IPHESYRLVY QIDGETVWIL TLVHTARLWP PVRD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | parD | ParD | Tn5501.6 | 92 | 1561-1839 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antitoxin | ||||||||||
| Sequence Family: | parD (PDB:4Q2U) | ||||||||||
| Comment: | RelB/ParD/CcdA/DinJ | ||||||||||
| Protein Sequence: | MSKQAVFTMK LEPELRAEFM AEAEAAHRPA SQVLRELMRE FVQRQRESRE YDEFLRRKVE AGRASMRAGL GRSNDEVEAE FAARRASVAS QA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn5501.6 | 192 | 2093-2671 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MRVSSDSDRQ STNLQRDALL AVGVDARHLF EDHASGAKDD RAGLARALEF VRPGDVLVVW KLDRLGRSLS HLLAIVTSLK EKQVAFRSLT ENLDTTTPSG EFLFQVFGAL AQYERALIQE RVVAGLAAAR KRGRIGGRPQ AITGEKLEAI VAALDGGMSK AAVCRNFGVK RTTLIETLAR VGWTGSRGAS SR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn5501.6 | 1009 | 2668-5697 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MTTKSERLTV LSDAEQEALY GLPDFDDAQR LEYLALTETE LALASSRPGL HAQVYCILQI GYFKAKHAFF RFDWSEVEHD CAFVLSRYFH GESFEHKPIS KHEHYTQREW IADLFGYRPW AAEFLPQLAQ QAAQTVRRDV MPGFIAAELI VWLNEHKIIR PGYTTLQELV SEALSAERRR LAGLLSEVLD ESAKAALGRL LVRDDTLSQL AALKQDAKDF GWRQMAHERE KRATLEPLHR IAKALLPKLG VSQQNLLYYA SLANFYTVHD LRNLKADQTY LYLLCYAWVR YRQLSDNLVD AMAYHMKQLE DESSAGAKQS FVAEQVRRQQ DTPQVGRLLS LYIDDSVPDP TPFGDVRQRA YKIMPRDTLQ TTAQRMSVKP VSKLALHWQA VDGLAERIRR HLRPLYVALD LAGTDPGSPW LVALAWAKDV FAKQQRLSQR PLAECPAATL PKRLRPYLLT FDADGKPTGL RADRYEFWLY RQVRKRFQSG ELYLDDSLQH RHFSDELVSL DEKADVLAQI DIPFLRQPLD AQLDALATEL RSQWLAFNRE LKQGKLTHLE YDKDTQKLTW RKPRGENQKA RERAFYEQLP FCDVADVFRF VNGQCQFLSA LTPLQPRYAK KVADADSLMA VIIAQAMNHG NQVMARTSDI PYHVLESAYQ QYLRHATLHA ANDCISNAIA ALPIFPYYSF DLDALYGAVD GQKFGVARPT VKARHSRKYF GRGKGVVAYT LLCNHVPLNG YLIGAHDYEA HHVFDIWYRN TSDIVPTAIT GDMHSVNKAN FAILHWFGLR FEPRFTDLGD QLKELYSADD PALYDQCLIR PAGRIDRDLI VSEKPNLDQI VATLGLKEMT QGTLIRKLCT YTAPNPTRRA VFEFDKLIRS IYTLRYLRDP QLERNVHRSQ NRIESYHQLR STIAQVGGKK ELTGRTDIEI EISNQCARLI ANAVIFYNSA ILSRLLMNYE ASGNAKAHAL LTQISPAAWR HILLNGHYTF QSDGKMIDLD ALVAGLELG |
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1. Subedi D, Vijay AK, Kohli GS, Rice SA, Willcox M.
Nucleotide sequence analysis of NPS-1 beta-lactamase and a novel integron (In1427)-carrying transposon in an MDR Pseudomonas aeruginosa keratitis strain.. J Antimicrob Chemother. 2018 Jun 1;73(6):1724-1726. doi: 10.1093/jac/dky073. PubMed ID:
29547943
.