Mobile element type:
Transposon
Name:
Tn5501.12
Synonyms:
Accession:
Tn5501.12-CP017294.1
Family:
Tn3
Group:
Tn3000
First isolate:
ND
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Pseudomonas aeruginosa PA83
Date of Isolation:
2017
Country:
Germany
Molecular Source:
plasmid unnamed1
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGATGTAA GCCGGAACCC CAGAAATTTC CGTCACCC |
| IRR | 7131-7168 | - | 38 | GGGGTTCTAA GCCAGAACCG CCGAAATTTC CGTCATCC |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | Tn5501.12 | 3342-3509 | + | 168 | CTGTTCAGGT AATGATGTAA ACGCGGCAAC CTCAAGGAGG TGTCGTAAAA CATTTGTTTT TCGACAGGCC TTCAACGGTC CTCTGCACCA ACCTCCGAGT GGCCGCAAAA TTGTGCGGAA AACTCTGTCG CCAGACGCTA CCATACGGAA ACCTCGTCTT AATGGTTT |
| res_site_I | Tn5501.12 | 3342-3371 | + | 30 | CTGTTCAGGT AATGATGTAA ACGCGGCAAC |
| res_site_II | Tn5501.12 | 3379-3409 | + | 31 | AGGTGTCGTA AAACATTTGT TTTTCGACAG G |
| res_site_III | Tn5501.12 | 3485-3509 | + | 25 | TACGGAAACC TCGTCTTAAT GGTTT |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| rpfG | Tn5501.12 | 168-1301 | - | Passenger Gene | Other |
| pDK1_p018 | Tn5501.12 | 1556-1948 | - | Passenger Gene | Hypothetical |
| pDK1_p017 | Tn5501.12 | 1933-2259 | - | Passenger Gene | Hypothetical |
| WP_000741275.1 | Tn5501.12 | 2316-2618 | - | Passenger Gene | Hypothetical |
| HTH_37 | Tn5501.12 | 2633-2968 | - | Passenger Gene | Antitoxin |
| Gp49 | Tn5501.12 | 2949-3326 | - | Passenger Gene | Toxin |
| tnpR | Tn5501.12 | 3532-4110 | + | Accessory Gene | Resolvase |
| tnpA | Tn5501.12 | 4107-7136 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | rpfG | RpfG | Tn5501.12 | 377 | 168-1301 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | variant of Tn4662a metal dependent phosphohydrolases with conservedHD motif | ||||||||||
| Protein Sequence: | MWRRGGLGRL GQKPSRPREP REPREPREPR EPRAQSPEPR AQSPEPRELS EEQSLPSSPL SKKSDGATSM ESEMCYARKL CLAAKSQVMD MFQEARLGKA VDPSTTLPLV GEIAASVLRQ PHALISVARI KTHDDYTYLH SVAVCALMLS LARHLDLDEE QTRLAGIGGL MHDLGKAAMP LEVLNKPGKL TDAEFAIMKR HPVEGAKMLR AGGAEPGVVD IALHHHEKID GTGYPDRLAG DAISLLARMG AICDVYDAVT SERAYKKPWD PSAAMRQMAK WEGHFDKRIF HAFVKAVGIY PVGSLVRLSS QRLAVVVEPG MESLLTPKVR VFFSLRSREP IPMQTIDLAA TSCKDSITGP EDPTLWNFKN LDDLWME |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | pDK1_p018 | PDK1_p018 | Tn5501.12 | 130 | 1556-1948 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Protein Sequence: | MDEQMNLSTI EALAIAWARI AEEAELPAGY EGTATPEAHR ACEVIQERIR EHVVATNDMR LFGLLHLLGQ ASLRMEQALW PEEYARMTRE VEEALREADD PNAKSYTHEE VMQAMQERID RARDKAMLIG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | pDK1_p017 | PDK1_p017 | Tn5501.12 | 108 | 1933-2259 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Protein Sequence: | MSRSRRKTPI VGHTTCRSER EDKKLWHQRW RTHERTALAS ASPEALCAHL PLLENQVSNV WSMGKDGRSY WPIKRQAATA DRIANHKGRN PQERASLKKR LLRKWMSK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | WP_000741275.1 | WP_000741275.1 | Tn5501.12 | 101 | 2316-2618 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Protein Sequence: | MAARITDDEW DELTPENFDT TALLRAVDAV DVLRGDLNDS ADGAPPQLRT DLLKLHQLAM AAFNEGSRSR VAELFDLAVD LQDQVDHLMT SLEQVQETLS R |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | HTH_37 | HTH_37 | Tn5501.12 | 111 | 2633-2968 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antitoxin | ||||||||||
| Sequence Family: | HTH_37 (Pfam:PF13744) | ||||||||||
| Protein Sequence: | MQKRIIEGVE VQRSSGNVFA DLGLPDAEKL KIKTGLVVEI RRAMRALGLT QQAAAKRMGI PQPKVSGMMR GDFTNLSERK LMDCLNRLGY DIEIKVRPAA EPIGHLTLAT A |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | Gp49 | Gp49 | Tn5501.12 | 125 | 2949-3326 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Toxin | ||||||||||
| Sequence Family: | Gp49 (Pfam:PF05973) | ||||||||||
| Target: | ribosome associated mRNA | ||||||||||
| Protein Sequence: | MTNKEKPLEW IASSHKDLMA LPSDVRRRFG YALSLAQIGD QDDAAKVLKG FGGAGVLEVV EDDAGGTYRA VYTVKFAEAV FVLHCFQKKS KSGIATPKAD MDIIRARLKV AEVLAQELRN AKTNH |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn5501.12 | 192 | 3532-4110 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MRVSSDSDRQ STNLQRDALL AVGVDARHLF EDHASGAKDD RAGLARALEF VRPGDVLVVW KLDRLGRSLS HLLAIVTSLK KKQVAFRSLT ENLDTTTPSG EFLFQVFGAL AQYERALIQE RVVAGLAAAR KRGRIGGRPQ AITGEKLEAI VAALDGGMSK AAVCRNFGVK RTTLIETLAR VGWTGSRGAS SR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn5501.12 | 1009 | 4107-7136 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MTTKSERLTV LSDAEQEALY GLPDFDDAQR LEYLALTETE LALASSRPGL HAQVYCILQI GYFKAKHAFF RFDWSEVEHD CAFVLSRYFH GESFEHKPIS KHEHYTQREW IADLFGYRPW AAEFLAQLAQ QAAQTVRRDV MPGFIAAELI VWLNEHKIIR PGYTTLQELV SEALSAERRR LAGLLSEVLD ESAKAALGRL LVRDDTLSQL AALKQDAKDF GWRQMARERE KRATLEPLHR IAKALLPKLG VSQQNLLYYA SLANFYTVHD LRNLKADQTY LYLLCYAWVR YRQLSDNLVD AMAYHMKQLE DESSAGAKQS FVAEQVRRQQ DTPQVGRLLS LYIDDSVPDP TPFGDVRQRA YKIMPRDTLQ TTAQRMSVKP VSKLALHWQA VDGLAERIRR HLRPLYVALD LAGTDPGSPW LVALAWAKDV FAKQQRLSQR PLAECPAATL PKRLRPYLLT FDADGKPTDL HADRYEFWLY RQVRKRFQSG ELYLDDSLQH RHFSDELVSL DEKAAVLAQI DIPFLRQPLD AQLDALATEL RAQWLAFNRE LKQGKLTHLE YDKDTQKLTW RKPKGENQKA REKAFYEQLP FCDVADVFRF VNGQCQFLSA LTPLQPRYAK KVADADSLMA VIIAQAMNHG NQVMARTSDI PYHVLESAYQ QYLRHATLHA ANDCISNAIA ALPIFPYYSF DLDALYGAVD GQKFGVERPT VKARHSRKYF GRGKGVVAYT LLCNHVPLNG YLIGAHDYEA HHVFDIWYRN TSDIVPTAIT GDMHSVNKAN FAILHWFGLR FEPRFTDLGD QLKELYSADD PALYDQCLIR PAGRIDRDLI VSEKPNLDQI VATLGLKEMT QGTLIRKLCT YTAPNPTRRA VFEFDKLIRS IYTLRYLRDP QLERNVHRSQ NRIESYHQLR STIAQVGGKK ELTGRTDIEI EISNQCARLI ANAVIFYNSA ILSRLLMKYE ASGNAKAHAL LTQISPAAWR HILLNGHYTF QSDGKMIDLD ALVAGLELG |
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1. Dosselmann B, Willmann M, Steglich M, Bunk B, Nubel U, Peter S, Neher RA.
Rapid and Consistent Evolution of Colistin Resistance in Extensively Drug-Resistant Pseudomonas aeruginosa during Morbidostat Culture.. Antimicrob Agents Chemother. 2017 Aug 24;61(9):e00043-17. doi: 10.1128/AAC.00043-17. Print 2017 Sep. PubMed ID:
28630206
.