Mobile element type:
Transposon
Name:
Tn5501.10
Synonyms:
Accession:
Tn5501.10-MH053445
Family:
Tn3
Group:
Tn3000
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Pseudomonas aeruginosa PA1280
Date of Isolation:
2017
Country:
China
Molecular Source:
plasmid pICP-4GES
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGTTCTAA GCCGGAACCG CCGAAAATTC CGTCAGCC |
| IRR | 6749-6786 | - | 38 | GGGGTTCTAA GCCAGAACCG CCGAAATTTC CGTCATCC |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | Tn5501.10 | 2997-3127 | + | 131 | GCCTGTCGGA AAACATTTGT TTTTCGACAG GCCTTCAACG GTCCTCTGCA CCAACCTCCG AGTGGCCGCA AAATTGTGCG GAAAACTCTG TCGCCAGACG CTACCATACG GAAACCTCGT CTTAATGGTT T |
| res_site_I | Tn5501.10 | 2997-3025 | + | 29 | GCCTGTCGGA AAACATTTGT TTTTCGACA |
| res_site_II | Tn5501.10 | 3059-3102 | + | 44 | TGGCCGCAAA ATTGTGCGGA AAACTCTGTC GCCAGACGCT ACCA |
| res_site_III | Tn5501.10 | 3103-3127 | + | 25 | TACGGAAACC TCGTCTTAAT GGTTT |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| lysR family | Tn5501.10 | 376-1239 | - | Passenger Gene | Other |
| PAU-1 | Tn5501.10 | 1307-2218 | + | Passenger Gene | Antibiotic Resistance |
| parE | Tn5501.10 | 2337-2621 | - | Passenger Gene | Toxin |
| parD | Tn5501.10 | 2618-2896 | - | Passenger Gene | Antitoxin |
| tnpR | Tn5501.10 | 3150-3728 | + | Accessory Gene | Resolvase |
| tnpA | Tn5501.10 | 3725-6754 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | lysR family | LysR family | Tn5501.10 | 287 | 376-1239 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Protein Sequence: | MDLPLNALRA FEVSARHLSF TRAGLELHLT QTAVSQHVKN LEDRLGKKLF RRIPRGLALT DEGAALLPVL TDAFERIGLT LEKVKTPHKK EVLSVGVVGT FAVGWLLPRL RDFQVKHPFI DLRLFSNNNR VDLAGDGLDF AIRFGDGHWH GTEASKLFSA PLAPVCAPFM ASRIHTPSDL ARVTLLRSYR ADEWTAWFQA VGLNPPHLTG PVFDSSLVLA DAAAQGTGVA LLPIRLFGRD LLGGRLVTLF DTRIETGSYW LTKLKPRKET DGMKAFRGWL EQECRDN |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | PAU-1 | PAU-1 | Tn5501.10 | 303 | 1307-2218 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Sequence Family: | Beta-lactamase2 (Pfam:PF13354) | ||||||||||
| Target: | piperacillin (ARO:0000078)||ampicillin (ARO:3000637) | ||||||||||
| Comment: | no match in CARD||PMID:31824180 | ||||||||||
| Protein Sequence: | MITHFQTVGT MKRRNFSVGM AFSAFGFSAI FPKKSFALGE QGQMTAVFQQ LEAKAQGRLG VHVIDTATGH EFGYRSDELF MMLSSFKLLA SALVLARADR GEESLTRRIR YRKQDLVPWS PVTEAYADGE GLTLAQLCHA TITTSDNTAG NLILASYGGP QALTQYARQL GDKITRLDRN EPDLNTRVEG GSLDTTSPRA MAMTMNKLLL GDALSPLSRN LLRQWLLENT TGGKRLKAGT PADWTVGDKT GTNKTDANDI GILLPPQGAP VLVTAYLADS TASSQIKDAT LAEVGRLTSI GIR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | parE | ParE | Tn5501.10 | 94 | 2337-2621 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Toxin | ||||||||||
| Sequence Family: | ParE_toxin (Pfam:PF05016) | ||||||||||
| Target: | DNA gyrase | ||||||||||
| Protein Sequence: | VRVVWTPEAQ QDRADVWDYI AADNPRAAAR MDEIFSDAAA RLIQHPMLGK PGKIPGTREL IPHESYRLVY QIDGETVWIL TLVHTARLWP PVRD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | parD | ParD | Tn5501.10 | 92 | 2618-2896 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antitoxin | ||||||||||
| Sequence Family: | parD (PDB:4Q2U) | ||||||||||
| Comment: | RelB/ParD/CcdA/DinJ | ||||||||||
| Protein Sequence: | MSKQAVFTMK LEPELRAEFM AEAEAAHRPA SQVLRELMRE FVQRQRESRE YDEFLRRKVE AGRASMRAGL GRSNDEVEAE FAARRASVAS QA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn5501.10 | 192 | 3150-3728 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MRVSSDSDRQ STNLQRDALL AVGVDARHLF EDHASGAKDD RAGLARALEF VRPGDVLVVW KLDRLGRSLS HLLAIVTSLK KKQVAFRSLT ENLDTTTPSG EFLFQVFGAL AQYERALIQE RVVAGLAAAR KRGRIGGRPQ AITGEKLEAI VAALDGGMSK AAVCRNFGVK RTTLIETLAR VGWTGSRGAS SR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn5501.10 | 1009 | 3725-6754 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MTTKSERLTV LSDAEQEALY GLPDFDDAQR LEYLALTETE LALASSRPGL HAQVYCILQI GYFKAKHAFF RFDWSEVEHD CAFVLSRYFH GESFEHKPIS KHEHYTQREW IADLFGYRPW AAEFLAQLAQ QAAQTVRRDV MPGFIAAELI VWLNEHKIIR PGYTTLQELV SEALSAERRR LAGLLSEVLD ESAKAALGRL LVRDDTLSQL AALKQDAKDF GWRQMARERE KRATLEPLHR IAKALLPKLG VSQQNLLYYA SLANFYTVHD LRNLKADQTY LYLLCYAWVR YRQLSDNLVD AMAYHMKQLE DESSAGAKQS FVAEQVRRQQ DTPQVGRLLS LYIDDSVPDP TPFGDVRQRA YKIMPRDTLQ TTAQRMSVKP VSKLALHWQA VDGLAERIRR HLRPLYVALD LAGTDPGSPW LVALAWAKDV FAKQQRLSQR PLAECPAATL PKRLRPYLLT FDADGKPTDL HADRYEFWLY RQVRKRFQSG ELYLDDSLQH RHFSDELVSL DEKAAVLAQI DIPFLRQPLD AQLDALATEL RAQWLAFNRE LKQGKLTHLE YDKDTQKLTW RKPKGENQKA REKAFYEQLP FCDVADVFRF VNGQCQFLSA LTPLQPRYAK KVADADSLMA VIIAQTMNHG NQVMARTSDI PYHVLESAYQ QYLRHATLHA ANDCISNAIA ALPIFPYYSF DLDALYGAVD GQKFGVERPT VKARHSRKYF GRGKGVVAYT LLCNHVPLNG YLIGAHDYEA HHVFDIWYRN TSDIVPTAIT GDMHSVNKAN FAILHWFGLR FEPRFTDLGD QLKELYSADD PALYDQCLIR PAGRIDRDLI VSEKPNLDQI VATLGLKEMT QGTLIRKLCT YTAPNPTRRA VFEFDKLIRS IYTLRYLRDP QLERNVHRSQ NRIESYHQLR STIAQVGGKK ELTGRTDIEI EISNQCARLI ANAVIFYNSA ILSRLLMKYE ASGNAKAHAL LTQISPAAWR HILLNGHYTF QSDGKMIDLD ALVAGLELG |
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1. Xu T, Wang J, Ying J, Zhu T, Liu Y, Xu L, Li P, Li P, Ying J, Li K, Yi H, Lu J, Hu Y, Zhou T, Bao Q.
Characterisation of a class 1 integron associated with the formation of quadruple bla(GES-5) cassettes from an IncP-1beta group plasmid in Pseudomonas aeruginosa.. Int J Antimicrob Agents. 2018 Oct;52(4):485-491. doi: 10.1016/j.ijantimicag.2018.07.002. Epub 2018 Jul 31. PubMed ID:
30012438
.