Mobile element type:
Transposon
Name:
Tn5406
Synonyms:
Accession:
Tn5406-AF186237
Family:
Tn554
Group:
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Staphylococcus aureus MRSA
Date of Isolation:
January 2011
Country:
Spain
Molecular Source:
chromosome
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| att | Tn5406 | 1-6 | + | 6 | GATGTA |
| att | Tn5406 | 5468-5473 | + | 6 | GATGTA |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpA | Tn5406 | 140-1225 | + | Transposase | |
| tnpB | Tn5406 | 1222-3120 | + | Transposase | |
| tnpC | Tn5406 | 3121-3504 | + | Accessory Gene | Helper |
| vgaALC (ARO:3002830) | Tn5406 | 3821-5395 | + | Passenger Gene | Antibiotic Resistance |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn5406 | 361 | 140-1225 | + |
|---|
| Class: | Transposase | ||||||||||
| Comment: | tyrosine recombinase XerD?||TIGR02225||phage integrase, N-terminal SAM-like domain||DNA breaking-rejoining enzyme C-terminal catalytic domain | ||||||||||
| Protein Sequence: | MKVQKIVVDN KAYPLFILLD EQFEVVEPVM SFIKYLDNTN KSPNTIKTYC YHLKLFYVFM QQRKISLDEI QFDELANFVG WLRNPTENAN VIDIHPKEAK REESTVNAIL NAVISYLEYL SRSGGFKMLD LFNETRGRNY KGFLHHVSKG KPYKRNVLKL REKKKMIRTL NHSEVKEIVE ACHTLRDKLI IMLMYEGGMR IGEVLSLRLA DIVTWDNQVH ITPREESLNG AYIKLRKERV IHVSKELMAL YTDYLVHEYY DELEHDYVFI ALKESHFGQP LKYQSVLDLV RRLNKRTGIV FTPHTLRHTH ATELIRNGWD AAYVQKRLGH AQVQTTLNTY VHLSNQDMKE EFKKYLEGRD K |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpB | TnpB | Tn5406 | 632 | 1222-3120 | + |
|---|
| Class: | Transposase | ||||||||||
| Comment: | C-terminal catalytic domain cd01187 | ||||||||||
| Protein Sequence: | MTMNTKRKII SSVAIGKKIA EMNSKLEGYW ADDRWDIRKC PLPSAIELSK SPSLRNRWVN FDRVENLWLR TELKFFFYYH MTNEVWNAKT VWIRKGTVIN KMLGFLDMKY PHIESITEVP IEKAMTEYRT YLVEQGVRIT TTNHKLNAKQ ERITVKANSY YVTNLKQFME FYEDYYFDGE EWEKDVWDRR KMNLPSDKVN PTQYEYLVSF KEIPSIYYRE LTKRYCKLKL NTVSFSHVSD IAGRLKEFFV FLNKNYKHLT RLHQLTREQI EHYLAELNKS GIKPSTLMGK ISVLDGFFTT IQKFDWNDVP SKILVFQEDY PKVPKATPRY IDEYVLEQLN SHLDDLPAYI ATMVMIIQEG GMRISELCTL KRDCLLEDKE GDYFLKYYQW KMKKEHTIPI SREVAGLIKA HEKHVSEEFG GCEYLFPRKD GSPLKQDTFR RELNEVAHKK NIVDRAGSVF RFHAHAFRHT VGTRMINNGI PQHIVQKFLG HESPEMTSRY AHIFDETLKE EFSKFKETLV TNQGSIIDIE ESEEANKTDL QWFKKNINAQ VLPNGYCRLP IIAGPCPHAN ACLDCTHFCT SKKFLSQHKD HLAHTKELLA IAKEKQWQRQ IETNSRVQER LEQIIGSLKE TE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpC | TnpC | Tn5406 | 127 | 3121-3504 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Helper | ||||||||||
| Sequence Family: | Tn554_family | ||||||||||
| Protein Sequence: | MGKQSRNTDK IIQLAKEKSH NTRLNVEKII SKMSLEGKTI NFNTVAAEAK VSKSWLYKES DIRKQIENIR HRQLNGKRLN FTPIKKNKKS EEILIETLKA RIKELEEENG QLKNQVQKLY GELYFKD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vgaALC (ARO:3002830) | VgaALC | Tn5406 | 524 | 3821-5395 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target protection (ARO:0001003) | ||||||||||
| Sequence Family: | ABC-F ATP-binding cassette ribosomal protection protein (ARO:3004469) | ||||||||||
| Target: | streptogramin antibiotic (ARO:0000026)||pleuromutilin antibiotic (ARO:3000670)||lincosamide antibiotic (ARO:0000017)||oxazolidinone antibiotic (ARO:3000079)||tetracycline antibiotic (ARO:3000050)||phenicol antibiotic (ARO:3000387)||macrolideantibiotic (ARO:0000000) | ||||||||||
| Comment: | loose match to reference sequence for ARO:3002830 (bitscore: 833) | ||||||||||
| Protein Sequence: | MKILLEALRI KPYVQDRLLL NIDQLQIYQN DRIGLVGGNG SGKTTLLHIL DEKLFPEEGT VTQYSRCELI PQLKHMETTK SGGEVTRKYI QQALDKNPEL LLADEPTTNL DTNYIENLER KLKDWHGAFI IVSHDRAFLD TLCTSIWEIK DGGITEYKGN YSDYIEQKEL ENRQEQLAHE KYEKEKKQLE EAIKLKEEKA QRATKKPKNI SSSEARLKGA KPYFANKQKK LRKTAKALET RLDKLEKVEK TKELPPLKMD LLNSETFKNR IILRVEDVSG VIEERVLWKA ASFYVRGGDK LAIIGSNGTG KTTFIKKIVQ DKPGISFSPS VKIGYFSQNL DILELDKTIL ENVQSSSRQN ETLIRTILAR MHFFRDDVHK PVNVLSGGER VKVALTKVFL SDVNTLVLDE PTNFLDMEAI EAFESLLEEY EGSVIFVSHD RRFIEKIATR IMMIDNKEIK IFEGTYDQFK AKQAREETRD IKEDKKLLLE TRITEILSRL SIEPSEELEK EFQNLINEKR NLDN |
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1. Haroche J, Allignet J, Buchrieser C, El Solh N.
Characterization of a variant of vga(A) conferring resistance to streptogramin A and related compounds.. Antimicrob Agents Chemother. 2000 Sep;44(9):2271-5. doi: 10.1128/AAC.44.9.2271-2275.2000. PubMed ID:
10952567
.