Mobile element type:
Transposon
Name:
Tn5401
Synonyms:
Accession:
Tn5401-U03554.1
Family:
Tn3
Group:
Tn3
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Bacillus thuringiensis serovar morrisoni EG2158
Date of Isolation:
1994
Country:
U.s.a.
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-53 | + | 53 | GGGGTATGTG TAGCAATGGA ACAGAATCAC GCAACAAGCA TTAGCGGACA TTA |
| IRR | 4785-4837 | - | 53 | GGGGTATGTG TAGCAATGGA ACAAAATCAC GCAACAAGCA TTAGCGGACA TTA |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res_site_d | Tn5401 | 40-51 | - | 12 | ATGTCCGCTA AT |
| res | Tn5401 | 639-737 | + | 99 | ATGTCCACTA ATTAATATTA GTGGACATGA AGTGTGGGAA AATAAATGTT TGATGTCCGC TAACATAATT GATAAGATTA AAATATCATG TCCGCTAAT |
| res_site_a | Tn5401 | 639-650 | + | 12 | ATGTCCACTA AT |
| res_site_b | Tn5401 | 655-666 | - | 12 | ATGTCCACTA AT |
| res_site_c | Tn5401 | 691-702 | + | 12 | ATGTCCGCTA AC |
| res_site_d | Tn5401 | 726-737 | + | 12 | ATGTCCGCTA AT |
| res_site_d | Tn5401 | 4787-4798 | + | 12 | ATGTCCGCTA AT |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| parE | Tn5401 | 119-343 | - | Passenger Gene | Toxin |
| parD-noDBD | Tn5401 | 351-608 | - | Passenger Gene | Antitoxin |
| tnpI | Tn5401 | 764-1684 | + | Accessory Gene | Resolvase |
| tnpA | Tn5401 | 1756-4773 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | parE | ParE | Tn5401 | 74 | 119-343 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Toxin | ||||||||||
| Function: | unknown | ||||||||||
| Sequence Family: | ParE_toxin (Pfam:PF05016) | ||||||||||
| Target: | DNA gyrase | ||||||||||
| Protein Sequence: | MKFIAKQEKG IQKRIAEGLK GLLKIPPEGD IKSMKGYTEL YRLRIGTFRI LFEINHDEKV IYIQAIGNRG DIYK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | parD-noDBD | ParD-noDBD | Tn5401 | 85 | 351-608 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antitoxin | ||||||||||
| Function: | unknown | ||||||||||
| Sequence Family: | parD (PDB:4Q2U) | ||||||||||
| Comment: | no PBD | ||||||||||
| Protein Sequence: | MAIRKDELYR LIDHLDQQDE KAAFDFLEFL VQRSRRKPKE WEKIDMADPD HEPLSTQELE QLNSEEGYVS GEDAKREFGL QIDLP |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpI | TnpI | Tn5401 | 306 | 764-1684 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | cointegrate resolution, transcriptional regulation | ||||||||||
| Sequence Family: | Tyrosine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Tyrosine | ||||||||||
| Comment: | belongs to the phage integrase family of site-specific recombinases | ||||||||||
| Protein Sequence: | MHSTKTISIQ ATSLISDFIS SLSQEGDLHT KTLKEYTSDL KDFVFWFENV WGKHAEDTLF HPIEVTARTI ARYRGHMQVT RLLKPSTINR RINSIKRYFD WAKQKGLVQT NYSKSIKFVP TEKTSPKRMS DKEEAALMHA VEKYGTLRDR AMIIFMLHTG LRSMEVCDVQ IEDVIMRKRG GYVVVRSGKR NKQREVPLNS TARCALEEHI RLSEISQSYL FPSSKTGKRL QERAIRHILQ KYIRLAKLEG FSAHDLRHRF GYVMAERTPL HRLAQIMGHD NLNTTMIYVR ATQEDLQGEV EKIAWN |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn5401 | 1005 | 1756-4773 | + |
|---|
| Class: | Transposase | ||||||||||
| Function: | cointegrate formation during transposition | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPVDFLTPEQ EEKYGCFCDT PTSEQLAKYF WLDDTDKELI WNRRGEHNQL GFAVQLGTVR FLGTFLSDPT NVPQSVITYM ANQLHLDAQS FSRYRNKRSQ WDQMQEIRSV YGYKNFTDKS THWRFIRWLY ARAWLYNERP SVLFDLATAR CIEQKILLPG VSVLTRLVST VRDRSAENIW KKLSSLPDNV QKKQLENLLQ IDQKTKKTYL ERLSNPPVPI SVTGIKNTLI RLQELRQLNT ENWDMSRIPS KRLQQFARHT VAVRSQAIAR MPDQRRMAML VAFAKMYTQS AQDDVIDIFD RYLTDLFAKT YRKEQKERLR TIKDLDKAAR QLREACVILL EHTDPSVHPK TAVFEKISEK DLIQAVQIVD SLTYSPNQTL AYSGLLQHYG IIRKFLPLLM EEIELQATPA GLPILQAWNF VKEHGKSNKK RWKNAPLAGL NANWSKVVID KDSGTVNHRA YTFWMLEQVL EALHRHDLYI VGSEKYGDLR AQLLQDEEWK SIRPSILRSL DWSIDSYESL TPLKEELDKT YHQVIENWEN NPAVQIDTFA GKERIVLTPL DKQPEPESLQ KLKQQIHTML PNIDIPQLLL EVNRWTGFMD GFRHISEAKS RINELPISIC ALLISQACNI GLRPLVQDGV PSLERDRLTW IEQNYFRAET LSESNAKLVD FHSQLQLAKM WGGGEIASAD GLRFITPVKS VHTGPNPKYF GSGRGVTYYN YTSDQFTGLH GLVIPGTIRD SLYLLQCVLE QNTNLQPKEI MTDTAGYSDI IFGLFGLLGY QFSPRLADIS ESRLWRFDAN SDYSMLNNLS KSRIREELIH RHWEDMLRVA GSLKLNKINA THLIQALQYN GKPTMLGRAI GELGRLFKTR YLLLYLHDEN YRRKILNQLN RGEARHSLAR AVFYGKRGEL HQSYREGQEE QLGALGLVVN AIIVWNTRYI ESALQVLRNR GHTIDNDDIS RLSPLGHKHI NIVGRYSFVL PEEVKDGQLR TLTYEETNKK EPDSL |
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1. Baum JA.
Tn5401, a new class II transposable element from Bacillus thuringiensis.. J Bacteriol. 1994 May;176(10):2835-45. doi: 10.1128/jb.176.10.2835-2845.1994. PubMed ID:
7514590
.