Mobile element type:  Transposon
Name:  Tn5393.8
Synonyms: 
Accession:  Tn5393.8-CP002090.1
Family:  Tn3
Group:  Tn163
First isolate:  ND
Partial:  ND
Evidence of transposition:  ND

Host Organism:  Salmonella enterica subsp. enterica serovar Kentucky
Date of Isolation: 
Country: 
Molecular Source:  plasmid pCS0010A

Name Coordinates Direction Length Sequence
IRLl 1-81 + 81 GGGGTCGTTT GCGGGAGAGG GCGAAATCCT ACGCTAAGGC TTTGGCCAAC GATATTCTCC GGTAAGATTG ATGTGTTCCC A
IRL 1-40 + 40 GGGGTCGTTT GCGGGAGAGG GCGAAATCCT ACGCTAAGGC
IRRl 15781-15861 - 81 GGGGTCGTTT GCGGGAGGGG GCGGAATCCT ACGCTAAGGC TTTGGCCAGC GATATTCTCC GGTGAGATTG ATGTGTTCCC A
IRR 15822-15861 - 40 GGGGTCGTTT GCGGGAGGGG GCGGAATCCT ACGCTAAGGC

Name Associated TE Coordinates Orientation Length Sequence
res Tn5393.8 3565-3786 + 222 TGTCCCGTTC GACACCTGCG GCGCGCAAGG CGTCGTGCTG CAGGTCGAGA GACTGCGAGC CATCGGCTTT GGAGACGCGG GCATATCCGA TCAGCATGTA TCACAAACGT TGGTTTGAGG CGGCGCTTCG GCCACGATTG CATTGACCTC TGGAAATGTA TCTCAACCAG CTTCATAAAC AAAGCGTCTT GAACGCTATC AGATTTTGAA AAAGGAACAT GT

ORF Summary
Name Associated TE Coordinates Orientation Class Subclass
APH(6)-Id (ARO:3002660) Tn5393.8 107-952 - Passenger Gene Antibiotic Resistance
APH(3'')-Ib (ARO:3002639) Tn5393.8 943-1746 - Passenger Gene Antibiotic Resistance
orfAB IS1133 1853-2982 - Transposase
orfB IS1133 1853-2710 - Transposase
orfA IS1133 2707-2982 - Accessory Gene Regulator
tnpR Tn5393.8 3050-3661 - Accessory Gene Resolvase
tnp IS10R 4016-5224 + Transposase
tetD Tn10 5234-5650 - Passenger Gene Antibiotic Resistance
tetC Tn10 5738-6331 + Passenger Gene Antibiotic Resistance
tet(B) (ARO:3000166) Tn10 6444-7649 - Passenger Gene Antibiotic Resistance
tetR (ARO:3003479) Tn10 7728-8354 + Passenger Gene Antibiotic Resistance
jemC Tn10 8332-9018 - Passenger Gene Other
CPT07_26605 Tn10 9026-9412 - Passenger Gene Hypothetical
jemB Tn10 9405-9725 - Passenger Gene Other
jemA Tn10 10169-11374 + Passenger Gene Other
tnp IS10L 11740-12948 - Transposase
tnpA Tn5393.8 13000-15828 + Transposase None
ORF Details
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
APH(6)-Id (ARO:3002660) APH(6)-Id Tn5393.8 281 107-952 -
Class: Passenger Gene
Subclass: Antibiotic Resistance
Function: antibiotic inactivation (ARO:0001004)
Sequence Family: APH(6) (ARO:3000151)
Target: aminoglycoside antibiotic (ARO:0000016)
Comment: strB, orfI || strict match to reference sequence for ARO:3002660 (bitscore: 568)
Protein Sequence: MGLMFMPPVF PAHWHVSQPV LIADTFSSLV WKVSLPDGTP AIVKGLKPIE DIADELRGAD YLVWRNGRGA VRLLGRENNL MLLEYAGERM LSHIVAEHGD YQATEIAAEL MAKLYAASEE PLPSALLPIR DRFAALFQRA RDDQNAGCQT DYVHAAIIAD QMMSNASELR GLHGDLHHEN IMFSSRGWLV IDPVGLVGEV GFGAANMFYD PADRDDLCLD PRRIAQMADA FSRALDVDPR RLLDQAYAYG CLSAAWNADG EEEQRDLAIA AAIKQVRQTS Y

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
APH(3'')-Ib (ARO:3002639) APH(3'')-Ib Tn5393.8 267 943-1746 -
Class: Passenger Gene
Subclass: Antibiotic Resistance
Function: antibiotic inactivation (ARO:0001004)
Sequence Family: APH(3'') (ARO:3000127)
Target: aminoglycoside antibiotic (ARO:0000016)
Comment: strA, orfH || perfect match to referencesequence for ARO: 3002639
Protein Sequence: MNRTNIFFGE SHSDWLPVRG GESGDFVFRR GDGHAFAKIA PASRRGELAG ERDRLIWLKG RGVACPEVIN WQEEQEGACL VITAIPGVPA ADLSGADLLK AWPSMGQQLG AVHSLSVDQC PFERRLSRMF GRAVDVVSRN AVNPDFLPDE DKSTPQLDLL ARVERELPVR LDQERTDMVV CHGDPCMPNF MVDPKTLQCT GLIDLGRLGT ADRYADLALM IANAEENWAA PDEAERAFAV LFNVLGIEAP DRERLAFYLR LDPLTWG

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
orfAB OrfAB IS1133 376 1853-2982 -
Class: Transposase
Transposase Chemistry: DDE
Comment: fusion protein from -1 programmed frameshifting between orfA and orfB
Protein Sequence: MSLKHSDEFK RDAVRIALTS GLTRRQVASD LSIGLSTLGK WIASISDETK IPTQDTDLLR ENERLRKENR ILREEREILK KAAIFFAVQK L*DFSLLRIT VALSHVHAYV V*WA*QIVVY VHGNAVLHHC ASVVILYF*R IYVSSIGCVW GAMVGRV*QK S*KRWACRLG SVGLDV*CAR ITLQLFERVN SNGQRIVIIP STLHRTY*NK TLAQAHPTRN GQAISLMFGP EKDGSILLLS LTCIPVA*LA GQQVID*SRI LH*GH*IWRW LYANHHRVVF NTQTVGANIA LMNIKSYCSN INCCRP*AGK AIVLITPQ*K ASLNH*RLS* FGADTGKQGE ILRLQSSNI* MAFIIHAEDI QHSAGNRRWH LRKKPL

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
orfB OrfB IS1133 285 1853-2710 -
Class: Transposase
Function: Nucleic acid binding (GO:0003676)
Transposase Chemistry: DDE
Comment: frameshiftrequired| 5'-end of tnp gene
Protein Sequence: MRFQFITDYR GSLSRSRICR LMGVTDRGLR AWKRRPPSLR QRRDLILLAH IREQHRLCLG SYGRPRMTEE LKALGLQVGQ RRVGRLMRQN NITVVRTRKF KRTTDSHHTF NIAPNLLKQD FSASAPNQKW AGDITYVWTR EGWVYLAVIL DLYSRRVIGW ATGDRLKQDL ALRALNMALA LRKPPPGCIQ HTDRGSQYCA HEYQKLLLKH QLLPSMSGKG NCFDNSAVES FFKSLKAELI WRRHWQTRRD IEIAIFEYIN GFYNPRRRHS TLGWKSPVAF EKKAA

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
orfA OrfA IS1133 91 2707-2982 -
Class: Accessory Gene
Subclass: Regulator
Function: transposase activity (GO:0004803)
Comment: orfA, together with orfB, encodes a fusion protein formed by translational frame-shifting that is the transposase forIS1133
Protein Sequence: MSLKHSDEFK RDAVRIALTS GLTRRQVASD LSIGLSTLGK WIASISDETK IPTQDTDLLR ENERLRKENR ILREEREILK KAAIFFAVQK L

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpR TnpR Tn5393.8 204 3050-3661 -
Class: Accessory Gene
Subclass: Resolvase
Function: recombinaseactivity (GO:0000150)
Sequence Family: Serine Site-Specific Recombinase
Transposase Chemistry: Serine
Protein Sequence: MLIGYARVSK ADGSQSLDLQ HDALRAAGVE RDNIYDDLAS GGRDDRPGLT ACLKSLRDGD VLVVWKLDRL GRSLAHLVNT VKELSDRKIG LRVLTGKGAQ IDTTTASGRM VFGIFATLAE FERDLIRERT MAGLASARAR GRKGGRKFAL TKAQVRLAQA AMAQRDTSVS DLCKELGIER VTLYRYVGPK GELRDHGKHV LGLT

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnp Tnp IS10R 402 4016-5224 +
Class: Transposase
Function: transposase
Transposase Chemistry: DDE
Comment: minor sequence variant-99.9% identical to reference sequence for tnp (IS10R)
Protein Sequence: MCELDILHDS LYQFCPELHL KRLNSLTLAC HALLDCKTLT LTELGRNLPT KARTKHNIKR IDRLLGNRHL HKERLAVYRW HASFICSGNT MPIVLVDWSD IREQKRLMVL RASVALHGRS VTLYEKAFPL SEQCSKKAHD QFLADLASIL PSNTTPLIVS DAGFKVPWYK SVEKLGWYWL SRVRGKVQYA DLGAENWKPI SNLHDMSSSH SKTLGYKRLT KSNPISCQIL LYKSRSKGRK NQRSTRTHCH HPSPKIYSAS AKEPWVLATN LPVEIRTPKQ LVNIYSKRMQ IEETFRDLKS PAYGLGLRHS RTSSSERFDI MLLIALMLQL TC*LAGVHAQ KQGWDKHFQA NTVRNRNVLS TVRLGMEVLR HSGYTITRED LLVAATLLAQ NLFTHGYALG KL

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tetD TetD Tn10 138 5234-5650 -
Class: Passenger Gene
Subclass: Antibiotic Resistance
Target: tetracycline antibiotic (ARO:3000050)
Comment: no match in CARD
Protein Sequence: MYIEQHSRYQ NKANNIQLRY DDKQFHTTVI KDVLLWIEHN LDQSLLLDDV ANKAGYTKWY FQRLFKKVTG VTLASYIRAR RLTKAAVELR LTKKTILEIA LKYQFDSQQS FTRRFKYIFK VTPSYYRRNK LWELEAMH

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tetC TetC Tn10 197 5738-6331 +
Class: Passenger Gene
Subclass: Antibiotic Resistance
Function: DNA binding (GO:0003677)
Sequence Family: Bacterial regulatory proteins, tetR family (Pfam:PF00440)
Target: tetracycline antibiotic (ARO:3000050)
Comment: no match inCARD||99.8% identical to TnCentral reference sequence for tetC (Tn10)
Protein Sequence: MENKNHQQEN FKSTYQSLVN SARILFVEKG YQAVSIDEIS GKALVTKGAF YHHFKNKKQL LSACYKQQLI MIDAYITTKT DLTNGWSALE SIFEHYLDYI IDNNKNLIPI QEVMPIIGWN ELEKISLEYI TGKVNAIVSK LIQENQLKAY DDDVLKNLLN GWFMHIAIHA KNLKELADKK GQFIAIYRGF LLSLKDK

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tet(B) (ARO:3000166) Tet(B) Tn10 401 6444-7649 -
Class: Passenger Gene
Subclass: Antibiotic Resistance
Function: antibiotic efflux (ARO:0010000)
Sequence Family: major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002)
Transposase Chemistry: antibiotic efflux (ARO:0010000)
Target: tetracycline antibiotic (ARO:3000050)
Comment: strict match to reference sequence for ARO:3000166 (bitscore:786)||99.9% identical to TnCentral reference sequence for tet(B) (Tn10)||Synonym: tetB
Protein Sequence: MNSSTKIALV ITLLDAMGIG LIMPVLPTLL REFIASEDIA NHFGVLLALY ALMQVIFAPW LGKMSDRFGR RPVLLLSLIG ASLDYLLLAF SSALWMLYLG RLLSGITGAT GAVAASVIAD TTSASQRVKW FGWLGASFGL GLIAGPIIGG FAGEISPHSP FFIAALLNIV AFLVVMFWFR ETKNTRDNTD TEVGVETQSN SVYITLFKTM PILLIIYFSA QLIGQIPATV WVLFTENRFG WNSMMVGFSL AGLGLLHSVF QAFVAGRIAT KWGEKTAVLL GFIADSSAFA FLAFISEGWL VFPVLILLAG GGIALPALQG VMSIQTKSHQ QGALQGLLVS LTNATGVIGP LLFAVIYNHS LPIWDGWIWI IGLAFYCIII LLSMTFMLTP QAQGSKQETS A

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tetR (ARO:3003479) TetR Tn10 208 7728-8354 +
Class: Passenger Gene
Subclass: Antibiotic Resistance
Function: antibiotic target alteration (ARO:0001001)||antibiotic efflux (ARO:0010000)
Sequence Family: major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002)
Transposase Chemistry: repressor of the tetracycline resistance element
Target: tetracycline antibiotic (ARO:3000050)
Comment: perfect match to reference sequence for ARO:3003479
Protein Sequence: MMSRLDKSKV INSALELLNE VGIEGLTTRK LAQKLGVEQP TLYWHVKNKR ALLDALAIEM LDRHHTHFCP LEGESWQDFL RNNAKSFRCA LLSHRDGAKV HLGTRPTEKQ YETLENQLAF LCQQGFSLEN ALYALSAVGH FTLGCVLEDQ EHQVAKEERE TPTTDSMPPL LRQAIELFDH QGAEPAFLFG LELIICGLEK QLKCESGS

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
jemC JemC Tn10 228 8332-9018 -
Class: Passenger Gene
Subclass: Other
Sequence Family: HTH ArsR-type DNA-binding domain (InterPro:IPR001845)
Comment: N-terminal 100 amino acids similar to bacterial transcriptional repressors and metal-binding proteins (PMID:10781570)
Protein Sequence: MANLIRKEVT FESSIAAIGA AMSDISRVKI LSALMDGRAW TATELSSVAN ISASTASSHL SKLLDCQLIT VVAQGKHRYF RLAGKDIAEL MESMMGISLN HGVHAKVSTP VHLRKARTCY DHLAGEVAVK IYDSLCQQQW ITENGSMITL SGIQYFHEMG IDVPSKHSRK ICCACLDWSE RRFHLGGYVG AALFSLYESK GWLTRHLGYR EVTITEKGYA AFKTHFHI

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
CPT07_26605 CPT07_26605 Tn10 128 9026-9412 -
Class: Passenger Gene
Subclass: Hypothetical
Comment: Amino acid-binding protein
Protein Sequence: MFDVHVVLDN QIGQLALLGK TLGNKGIGLE GGGIFTVGDE CHAHFLVEQG KEAKIALEQA GLLVLAIRTP LIRKLKQEKP GELGEIARVL AENNINILVQ YSDHANQLIL ITDNDSMAAS VTLPWAIK

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
jemB JemB Tn10 106 9405-9725 -
Class: Passenger Gene
Subclass: Other
Sequence Family: Antibiotic biosynthesis monooxygenase (Pfam:PF03992)
Protein Sequence: MIAVIFEVQI QPDQQTRYLT LAEELRPLLS HVAGFISIER FQSLATEGKM LSLSWWENEY AVLQWKNHVL HAKAQQEGRE SIFDFYKISI AHITREYSFK KDKDNV

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
jemA JemA Tn10 401 10169-11374 +
Class: Passenger Gene
Subclass: Other
Function: glutamate:sodium symporter activity (GO:0015501)
Comment: sodium-dependent glutamate permease
Protein Sequence: MILDASYTLL VACIALLIGM FVVKFTPFLQ KNHIPEAVVG GFIVAIVLLI IDKTSGYSFT FDASLQSLLM LTFFSSIGLS SDFSRLIKGG KPLVLLTIAV TILIAIQNTV GMSMAVMMNE SPFIGLIAGS ITLTGGHGNA GAWGPILADK YDVTGAVELA MACATLGLVL GGLVGGPVAR HLLKKVSIPK TTEQERDTIV EAFEQPSVKR KINANNVIET ISMLIICIVV GGYISALFKD TFLQLPTFVW CLFVGIIIRN TLTHVFKHEV FEPTVDVLGS VALSLFLAMA LMSLKFGQLA SMAGPVLIII AVQTVVMVLF ACFVTFKMMG KDYDAVVISA GHCGFGMGAT PTAIANMQTV TKAFGPSHKA FLVVPMVGAF IVDISNSILI KIFIEIGTYF T

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnp Tnp IS10L 402 11740-12948 -
Class: Transposase
Function: transposase
Transposase Chemistry: DDE
Protein Sequence: MCELDILHDS LYQFCPELHL KRLNSLTLAC HALLDCKTLT LTELGRNLPT KARTKHNIKR IDRLLGNRHL HKERLAVYRW HASFICSGNT MPIVLVDWSD IREQKRLMVL RASVALHGRS VTLYEKAFPL SEQCSKKAHD QFLADLASIL PSNTTPLIVS DAGFKVPWYK SVEKLGWYWL SRVRGKVQYA DLGAENWKPI SNLHDMSSSH SKTLGYKRLT KSNPISCQIL LYKSRSKGRK NQRSTRTHCH HPSPKIYSAS AKEPWILATN LPVEIRTPKQ LVNIYSKRMQ IEETFRDLKS PAYGLGLRHS RTSSSERFDI MLLIALMLQL TCWLAGVHAQ KQGWDKHFQA NTVRNRNVLS TVRLGMEVLR HSGYTITRED SLVAATLLTQ NLFTHGYVLG KL

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpA TnpA Tn5393.8 942 13000-15828 +
Class: Transposase
Function: transposase activity (GO:0004803)
Transposase Chemistry: DDE
Comment: probably N-terminal truncation by Tn10 insertion
Protein Sequence: MCIYLNLMIL IKIIRGFISI RLRRRAHNRF GFALQLCVLR YPGRVLAPGE LIPAEVIEFI GAQLGLGADD LVDYAAREET RHEHLAELRG LYGFRTFSGR GASELKEWLF REAEMAVSNE DIARRFVAEC RRTRTVLPAT STIERLCAAA LVDAERRIET RIASRLPMSI REQLLALLEE TADDRVTRFV WLRQFEPGSN SSSANRLLDR LEYLQRIDLP EDLLAGVPAH RVTRLRRQGE RYYADGMRDL PEDRRLAILA VCVSEWQAML ADAVVETHDR IVGRLYRASE RICHAKVADE AGVVRDTLKS FAEIGGALVD AQDDGQPLGD VIASGSGWDG LKTLVAMATR LTATMADDPL NHVLDGYHRF RRYAPRMLRL LDLRAAPVAL PLLEAVTALR TGLNDAAMTS FLRPSSKWHR HLRAQRAGDA RLWEIAVLFH LRDAFRSGDV WLTRSRRYGD LKHALVPAQS IAEGGRLAVP LRPEEWLADR QARLDMRLRE LGRAARAGTI PGGSIENGVL HIEKLEAAAP TGAEDLVLDL YKQIPPTRIT DLLLEVDAAT GFTEAFTHLR TGAPCADRIG LMNVILAEGI NLGLRKMADA TNTHTFWELI RIGRWHVEGE AYDRALAMVV EAQAALPMAR FWGMGTSASS DGQFFVATEQ GEAMNLVNAK YGNTPGLKAY SHVSDQYAPF ATQVIPATAS EAPYILDGLL MNDAGRHIRE QFTDTGGFTD HVFAACAILG YRFAPRIRDL PSKRLYAFNP SAAPAHLRAL IGGKVNQAMI ERNWPDILRI AATIAAGTVA PSQILRKLAS YPRQNELATA LREVGRVERT LFMIDWILDA ELQRRAQIGL NKGEAHHALK RAISFHRRGE IRDRSAEGQH YRIAGMNLLA AIIIFWNTMK LGEVVANQKR DGKLLSPDLL AHVSPLGWEH INLTGEYRWP KP

TnCentral Accession TE Name Type Coordinates Strand Length
IS1133-Z12167 IS1133 insertion sequence 1815-3046 + 1232
Tn10-AF162223 Tn10 transposon 3909-13055 + 9147
IS10R-AF162223 IS10R insertion sequence 3909-5237 + 1329
IS10L-AF162223 IS10L insertion sequence 11727-13055 + 1329

Name Associated TE Coordinates Direction Length Sequence
IRR IS1133 1815-1841 + 27 TGGACTTGCA CCGGTTGTGT TCCGGTC
IRL IS1133 3020-3046 - 27 TGGACTTGCC TCGGTTTCGT TCCGGTC
IRL IS10R 3909-3925 + 17 CTGATGAATC CCCTAAT
IRR IS10R 5216-5237 - 22 CTGAGAGATC CCCTCATAAT TT
IRR IS10L 11727-11748 + 22 CTGAGAGATC CCCTCATAAT TT
IRL IS10L 13039-13055 - 17 CTGATGAATC CCCTAAT

1.  Johnson TJ, Thorsness JL, Anderson CP, Lynne AM, Foley SL, Han J, Fricke WF, McDermott PF, White DG, Khatri M, Stell AL, Flores C, Singer RS.  Horizontal gene transfer of a ColV plasmid has resulted in a dominant avian clonal type of Salmonella enterica serovar Kentucky..  PLoS One. 2010 Dec 22;5(12):e15524. doi: 10.1371/journal.pone.0015524.  PubMed ID: 21203520 .