Mobile element type:
Transposon
Name:
Tn512
Synonyms:
Accession:
Tn512-EU306744
Family:
Tn402
Group:
Tn5053
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Pseudomonas a
Date of Isolation:
1991
Country:
Australia
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRt | 1-25 | + | 25 | TGTCATTTTC AGAAGACGAC TGCAC |
| repeat t1 | 9-27 | + | 19 | TCAGAAGACG ACTGCACCA |
| repeat t2 | 49-67 | - | 19 | TCAGGAGTCG CCTGCACAT |
| IR Tn21-like | 8349-8386 | - | 38 | GGAGTCGCCT CAGAAAACGG AAAATAAAGC ACGCTAAG |
| repeat i4 | 8359-8377 | - | 19 | TCAGAAAACG GAAAATAAA |
| repeat i2 | 8401-8419 | + | 19 | CCGTGCGGTC GACTTTTGA |
| IRi | 8423-8450 | - | 28 | TGTCACTTTC AGAAGGCGAC AGCACGAA |
| repeat i1 | 8424-8442 | - | 19 | TCAGAAGGCG ACAGCACGA |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| repeat t3 | Tn512 | 78-97 | + | 20 | TCAGAAGTCA TCTGCACCAA |
| r5 | Tn512 | 3875-3888 | + | 14 | AATTTCTGTC ACAT |
| res | Tn512 | 3972-4006 | + | 35 | ACACTGTCAC ATAATCGAAC GTATATGTGA CAGGT |
| r2 | Tn512 | 3975-3988 | - | 14 | CGATTATGTG ACAG |
| r1 | Tn512 | 3991-4004 | + | 14 | CGTATATGTG ACAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tniA | Tn512 | 144-1826 | + | Transposase | |
| tniB | Tn512 | 1829-2737 | + | Accessory Gene | |
| tniQ | Tn512 | 2734-3951 | + | Accessory Gene | Target Site Selection |
| tniR | Tn512 | 4012-4626 | + | Accessory Gene | Resolvase |
| merE | Tn512 | 4679-4915 | - | Passenger Gene | Heavy Metal Resistance |
| merD | Tn512 | 4912-5277 | - | Passenger Gene | Heavy Metal Resistance |
| merA | Tn512 | 5294-6940 | - | Passenger Gene | Heavy Metal Resistance |
| merF | Tn512 | 6937-7182 | - | Passenger Gene | Heavy Metal Resistance |
| merP | Tn512 | 7185-7460 | - | Passenger Gene | Heavy Metal Resistance |
| merT | Tn512 | 7476-7826 | - | Passenger Gene | Heavy Metal Resistance |
| merR | Tn512 | 7898-8332 | + | Passenger Gene | Heavy Metal Resistance |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniA | TniA | Tn512 | 560 | 144-1826 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | homologous to TnsB of Tn7 | ||||||||||
| Protein Sequence: | MASDTSLIAE QGVATLPDAA WAQARQRAEI IGPLAALDVV GHEAADAAAH ALGLSRRQVY VLIRRARQGA GLVTDLARSR SGGGKGKGRL PESVERIIRE LLQKRFLTKQ KRSLAAFHRE VAQACKAQKL RAPARNTVAL RIAGLDPLKA TRRREGQDAS RSLQGVGGEP PAVTAPLEQV QIDHTVIDLI VVDERDRQPI GRPYLTIAID VFTRCVLGMV VTLEAPSSVS VGLCLVHVAC DKRPWLEGLN IEMEWPMSGK PRLLYLDNAA EFKSEALRRG CEQHGIRLDY RPLGQPHYGG IVERIIGTAM QMIHDELPGT TFSNPDQRGD YDSENKAALT LRELERWLTL AVGTYHGSVH NGLLQPPAAR WAEAIARTGV PTVITRTTAF LVDFLPIIRR TLTRTGFVID HIHYYADALK PWIARRDRLP AFLIRRDPRD ISRIWVLEPE GQHYLEIPYR TLSHPAVTLW EQRQALAKLR QQGREQVDES ALFRMIGQMR EIVSTAQKAT RKARRDADRR QHLKATAVLF KTTPPPDADM ADPQADNQPP AKPFDQIEEW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniB | TniB | Tn512 | 302 | 1829-2737 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | homologous to TnsC protein of Tn7 putative ATP-binding protein | ||||||||||
| Protein Sequence: | VEEYPIIDLS HLMPVAQGLA RLPADERIHR LRADRWIGYP RAVEALNRLE ALYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRTHPASSD ADQEHIPVLV VQMPSEPSVI RFYIALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRD AYLAIRSDDQ LENRFEPMML PVWEANDDCC SLLASFAASL PLRRPSSIAT LDMARYLLTR SEGTIGELAH LLMAAAVAAV ESGEEAINHR TLSMADYTGP SERRRQFERE LM |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniQ | TniQ | Tn512 | 405 | 2734-3951 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Target Site Selection | ||||||||||
| Protein Sequence: | MKPAPHWPLH PAPREGEALS SWLNRVALCY HMEVSELLEH DLGHGQVDDL DTAPPLALLA MLSQRSGIEP DRLRCMSFAG WVPWLLDSLD DQIPDALETY AFQLSVLLPK LRRRTRSITS WRAWLPSQPI HRACPLCLND PANQAVLLAW KLPLMLSCPL HGCWLESYWG VPGRFLGWEN ADTAPRTASD AIAVMDRRTW QALTTGHVEL PRRRIHAGLW FRLLRTLLDE LNTPLSTCGT CAGYLRQVWE GCGHPLRAGQ SLWRPYETLN PAVRLQMLEA AATAISLIEV RDISPPGEHA KLFWSEPQTG FTSGLSAKTP KPEPVDHWQR AVQAINEAII EARHNPETAR SLFALASYGR RDPASQEQLR ATFAKEGIPP EFLSHYEPDG PFACLRQSDG LNDKF |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniR | TniR | Tn512 | 204 | 4012-4626 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | also called TniC | ||||||||||
| Protein Sequence: | MLIGYMRVSK ADGSQATDLQ RDALIAAGVD PVHLYEDQAS GMREDRPGLT SCLKALRTGD TLVVWKLDRL GRDLRHLINT VHDLTGRGIG LKVLTGHGAA IDTTTAAGKL VFGIFAALAE FERELIAERT IAGLASARAR GRKGGRPFKM TAAKLRLAMA AMGQPETKVG DLCQELGVTR QTLYRHVSPK GELRPDGEKL LSRI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merE | MerE | Tn512 | 78 | 4679-4915 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | Broad-spectrum mercury transporter | ||||||||||
| Protein Sequence: | MNSPERLPSE KHQTITAYLW GALAVLTCPC HLPILAIVLA GTTAGAFIGE YWGIAALTLI GLFVLSVTRL LRPLIDRS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD | MerD | Tn512 | 121 | 4912-5277 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MSAYTVSRLA LDAGVSVHIV RDYLLRGLLR PVACTPGGYG LFDDAALQRL CFVRAAFEAG IGLYALARLC RALDAADSDE TAAQLVVLRQ FVERRREAMV DLEVQLVTMP TEPAQHAESL P |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA | MerA | Tn512 | 548 | 5294-6940 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercuric ion reductase | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MTEITVNGMT CTSCATHVKD ALEKIPGVNA AVVSYPESRA QVMADTAVSH NQLLAAIAAL GYQGSIRVGD FKDEPKIRDA LEGAGLHIAI IGSGGAAMAA ALKAVEQGAT VTLIERGTIG GTCVNIGCVP SKIMIRAAHI AHLRRESPFD GGIAATVPAI DRSKLLAQQQ ARVDELRHAK YEGILDGNPA ITVLHGEARF KDDQSLVVRL NEGGEREVTF DRCLVATGAS PAVPPIPGLK ESPYWTSTEA LVSDTIPARL AVIGSSVVAL ELAQAFARLG SQVTILARST LFFREDPAIG EAVTAAFRAE GIEVLEHTQA SQVAHVNGEF VLTTGHGELR ADKLLVATGR APNTRSLALD AAGVTVNAQG AIVIDQGMRT SNPNIYAAGD CTDQPQFVYV AAAAGTRAAI NMTGGDAALN LTAMPAVVFT DPQVATVGYS EAEAHHDGIE TDSRTLTLDN VPRALANFDT RGFIKLVIEE GSGRLIGVQA VAPEAGELIQ TAVLAIRNRM TVQELADQLF PYLTMVEGLK LAAQTFNKDV KQLSCCAG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merF | MerF | Tn512 | 81 | 6937-7182 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | mercuric ion transport protein | ||||||||||
| Protein Sequence: | MKDPKTLLRV SIIGTTLVAL CCFTPVLVIL LGVVSLSALT GYLDYVLLPA LAIFIGLTIY AIQRKRQADA CCTPKFIGVK K |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | Tn512 | 91 | 7185-7460 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | periplasmic mercuric ion binding protein | ||||||||||
| Protein Sequence: | MKKLLSALAL AAVVAPVWAA TQTVTLSVPG MTCSACPITV KKAISKVDGV SKVDVTFETR EAVVTFDDAK TSVQKLTKAT EDAGYPSSVK N |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | Tn512 | 116 | 7476-7826 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | mercury ion transport protein | ||||||||||
| Protein Sequence: | MSEPQNGRGA LFTGGLAAIL ASACCLGPLV LIALGFSGAW IGNLTVLEPY RPIFIGVALV ALFFAWRRIY RPSAACKPGE VCAIPQVRAT YKLIFWGVAV LVLVALGFPY VVPFFY |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR | MerR | Tn512 | 144 | 7898-8332 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | regulatory protein (repressor /inducer) | ||||||||||
| Protein Sequence: | MENNLENLTI GVFARTAGVN VETIRFYQRK GLLPEPDKPY GSIRRYGETD VTRVRFVKSA QRLGFSLDEI AELLRLEDGT HCEEASSLAE HKLKDVRERM ADLARMEAVL SDLVCACHAR KGNVSCPLIA SLQGKKEPRS ADAV |
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1. Petrovski S, Stanisich VA.
Tn502 and Tn512 are res site hunters that provide evidence of resolvase-independent transposition to random sites.. J Bacteriol. 2010 Apr;192(7):1865-74. doi: 10.1128/JB.01322-09. Epub 2010 Jan 29. PubMed ID:
20118251
.