Mobile element type:
Transposon
Name:
Tn5090
Synonyms:
Accession:
Tn5090-AM993098
Family:
Tn402
Group:
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Peudomonas aeruginosa
Date of Isolation:
2008
Country:
China
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRt | 1-33 | + | 33 | TGTCATTTTC AGAAGACGAC TGCACCAGTT GAT |
| IRi | 8012-8044 | - | 33 | TGTCGTTTTC AGAAGACGGC TGCACTGAAC GTC |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | Tn5090 | 3967-4001 | + | 35 | ACACTGTCAC ATAATTGAAC GTATACGTGA CGGGT |
| attC AAC(6')-Ib10 | Tn5090 | 5643-5708 , 6276-6282 | + | 73 | CGTTTGACAT GAGGGGCGAC CAAGGGCGCC AGCCCTTGGA CGTCCCCCTC GATGGAAGGG TTAGGCGCCT AAC |
| attC AAC(6')-Ib core | Tn5090 | 5643-5708 | + | 66 | CGTTTGACAT GAGGGGCGAC CAAGGGCGCC AGCCCTTGGA CGTCCCCCTC GATGGAAGGG TTAGGC |
| attI | Tn5090 | 6693-6748 | + | 56 | CTTTGTTTTA GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCA |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tniA | Tn5090 | 106-1821 | + | Transposase | |
| tniB | Tn5090 | 1824-2732 | + | Accessory Gene | |
| tniQ | Tn5090 | 2729-3946 | + | Accessory Gene | Target Site Selection |
| tniR | Tn5090 | 4008-4631 | + | Accessory Gene | Resolvase |
| VIM-17 (ARO:3002287) | Tn5090 | 4812-5612 | - | Passenger Gene | Antibiotic Resistance |
| AAC(6')-Ib10 (ARO:3002581) | Tn5090 | 5703-6257 | - | Passenger Gene | Antibiotic Resistance |
| dfrB1 (ARO:3002864) | Tn5090 | 6386-6679 | - | Passenger Gene | Antibiotic Resistance |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniA | TniA | Tn5090 | 571 | 106-1821 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MLNTRVHQSE VSMATDTPRI PEQGVATLPD EAWERARRRA EIISPLAQSE TVGHEAADMA AQALGLSRRQ VYVLIRRARQ GSGLVTDLVP GQSGGGKGKG RLPEPVERVI HELLQKRFLT KQKRSLAAFH REVTQVCKAQ KLRVPARNTV ALRIASLDPR KVIRRREGQD AARDLQGVGG EPPAVTAPLE ?VQIDHTVID LIVVDDRDRQ PIGRPYLTLA IDLFTRCVLG MVVTLEAPSA VSVGLCLVHV ACDKRPWLEG LNVEMDWQMS GKPLLLYLDN AAEFKSEALR RGCEQHGIRL DYRPLGQPHY GGIVERIIGT AMQMIHDELP GTTFSNPDQR GDYDSENKAA LTLRELERWL TLAVGTYHGS VHNGLLQPPA ARWAEAVARV GVPAVVTRAT SFLVDFLPIL RRTLTRTGFV IDHIHYYADA LKPWIARRER WPSFLIRRDP RDISRIWVLE PEGQHYLEIP YRTLSHPAVT LWEQRQALAR LRQLGREQVD ESALFRMIGQ MREIVSTAQK ATRKARRDAD RRQHLKLSPP PVKPTPPDAD SADSQVGNLP PAKPFEQIEE W |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniB | TniB | Tn5090 | 302 | 1824-2732 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Sequence Family: | ATP binding protein? | ||||||||||
| Comment: | similar function to Tn7 tnsC and MuB | ||||||||||
| Protein Sequence: | VDEYPCIDLS HLLPAAQGLA RLPASERIQR LRADRWIGYP RAVEALNRLE ALYAWPNKQR MPNLLLIGPT NNGKSMIVEK FRRTHPASSD ADQEHIPVLV VQMPSEPSVI RFYVALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRD AYLAIRSDDQ LENRFEPMML PVWEANDDCC SLLASFAASL PLRRPSPIAT LDMARYLLTR SEGTIGELAH LLMAAAIVAV ESGEEAINHR TLSMAVYTGP SERRRQFERE LM |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniQ | TniQ | Tn5090 | 405 | 2729-3946 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Target Site Selection | ||||||||||
| Comment: | identical to tniQ (Tn1721)|similar function to Tn7 tnsD? | ||||||||||
| Protein Sequence: | MKPAPRWPLH PAPKEGEALS SWLNRVALCY HMEEPDLLEH DLGHGQVDDL DTAPPLSLLA LLSQRSGIEL DRLRCMSFAG WVPWLLDSLD DQIPDALETY AFQLSVLLPR LRRKTRSITS WRAWLPSQPI NRACPLCLSD PENQAVLLAW KLPLMLSCPL HGCWLESYWG VPGRFLGWEN ADAEPRTASD AIAAMDQRTW QALTTGHVEL PRRRIHAGLW FRLLRTLLDE LNTPLSACGT CAGYPRQVWE GCGHPLRAGQ SLWRPYETLN PIVRLQMLEA AATAISLIEV RDISPPGEQA KLFWSEPQTG FTSGLPTKAP KPEPINHWQR AVQAIDEAII EARHNPETAR SLFALASYGR RDPASLERLR ATFVKEGIPP EFLSHYLPDA PFACLKQNDG LSDKF |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniR | TniR | Tn5090 | 207 | 4008-4631 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | resolution of cointegrates || Protein: ACE81792.1 || identical to tniR (Tn1721) | ||||||||||
| Protein Sequence: | MLIGYMRVSK ADGSQSTNLQ RDALIAAGVS LAHLYEDLAS GRRDDRPGLA ACLKALREGD TLIVWKLDRL ARDLRHLINT VHDLTARSVG LKVLTGHGAA VDTTTAAGKL VFGIFAALAE FERELISERT VAGLISARAR GRKGGRPFKM TAAKLRLAMA SMGQPETKVG DLCEELGITR QTLYRHVSPK GELRPDGVKL LSLGSAA |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | VIM-17 (ARO:3002287) | VIM-17 | Tn5090 | 266 | 4812-5612 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | VIM beta-lactamase (ARO:3000021) | ||||||||||
| Target: | cephamycin (ARO:0000044)||cephalosporin (ARO:0000032)||penam (ARO:3000008)||carbapenem (ARO:0000020)||penem (ARO:3003706) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3002287 (bitscore: 538) | ||||||||||
| Protein Sequence: | MFKLLSKLLV YLTASIMAIA SPLAFSVDSS GEYPTVSEIP VGEVRLYQIA DGVWSHIATQ SFDGAVYPSN GLIVRDGDEL LLIDTAWGAK NTAALLAEIE KQIGLPVTRA VSTHFHDDRV GGVDVLRAAG VATYASPSTR RLAEVEGNEI PTHSLEGLSS SGDAVRFGPV ELFYPGAAHS TDNLVVYVPS ASVLYGGCAI YELSRTSAGN VADADLAEWP TSIERIQQHY PEAQFVIPGH GLPGGLDLLK HTTNVVKAHT NRSVVE |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | AAC(6')-Ib10 (ARO:3002581) | AAC(6')-Ib10 | Tn5090 | 184 | 5703-6257 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | AAC(6') (ARO:3000345) | ||||||||||
| Transposase Chemistry: | aminoglycoside acetyltransferase | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3002581 (bitscore: 377) | ||||||||||
| Protein Sequence: | MTNSNDSVTL RLMTEHDLAM LYEWLNRSHI VEWWGGEEAR PTLADVQERY LPSVLAQESV TPYIAMLNGE PIGYAQSYVA LGSGDGWWEE ETDPGVRGID QSLANASQLG KGLGTKLVRA LVELLFNDPE VTKIQTDPSP SNLRAIRCYE KAGFERQGTV TTPDGPAVYM VQTRQAFERT RSDA |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | dfrB1 (ARO:3002864) | DfrB1 | Tn5090 | 97 | 6386-6679 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target replacement (ARO:0001002) | ||||||||||
| Sequence Family: | trimethoprim resistant dihydrofolate reductase dfr (ARO:3001218) | ||||||||||
| Target: | diaminopyrimidine antibiotic (ARO:3000171) | ||||||||||
| Comment: | 98% identity with reference sequence ARO:3002864 from Escherichia coli (bitscore: 200)||Synonyms: dfr2a dfrII | ||||||||||
| Protein Sequence: | MQRVVGPHRT PRSSQERSEM ERSSNEVSNP VAGNFVFPSN ATFGIGDRVR KKSGAAWQGQ IVGWYCTNLT PEGYAVESEA HPGSVQIYPV AALERIN |
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