Mobile element type:  Transposon
Name:  Tn5086
Synonyms: 
Accession:  Tn5086-CP054343
Family:  Tn3
Group:  Tn21
First isolate:  Yes
Partial:  ND
Evidence of transposition:  ND

Host Organism:  Escherichia coli SCU-164
Date of Isolation:  2020
Country:  Usa
Molecular Source:  chromosome

Name Coordinates Direction Length Sequence
repeat i4 10-28 + 19 TCAGAAAACG GAAAATAAA
IRR 15304-15341 - 38 GGGGTCGTCT CAGAAAACGG AAAATAAAGC ACGCTAAG
repeat Tn21 15314-15332 - 19 TCAGAAAACG GAAAATAAA

Name Associated TE Coordinates Orientation Length Sequence
attC-qacE2 3'-end In22 7418-7423 + 6 TTAGAT
attC qacH_sul1 In22 8018-8051 , 9329-9335 + 41 CCGCTAGCGG GCGGCCGGAA GGTGAATGCT AGGCATCTAA C
attC qacEdelta1_sul1 core In22 8018-8051 + 34 CCGCTAGCGG GCGGCCGGAA GGTGAATGCT AGGC
attC dfrA7 core In22 9353-9416 + 64 TTTTTAAGTT GTGGTTTTAT GGAATACTTT TGCGCAGCAA AACCATAAAA CCGCGACTTA AAAA
attI In22 9951-10006 + 56 CTTTGTTTTA GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCA
res Tn5086 11638-11768 + 131 GCCGCCGTCA GGTTGAGGCA TACCCTAACC TGATGTCAGA TGCCATGTGT AAATTGCGTC AGGATAGGAT TGAATTTTGA ATTTATTGAC ATATCTCGTT GAAGGTCATA GAGTCTTCCC TGACATTTTG C
res_site_I Tn5086 11638-11676 + 39 GCCGCCGTCA GGTTGAGGCA TACCCTAACC TGATGTCAG
res_site_II Tn5086 11690-11733 + 44 ATTGCGTCAG GATAGGATTG AATTTTGAAT TTATTGACAT ATCT
res_site_III Tn5086 11737-11768 + 32 TGAAGGTCAT AGAGTCTTCC CTGACATTTT GC

ORF Summary
Name Associated TE Coordinates Orientation Class Subclass
merR Tn5086 34-468 - Passenger Gene Heavy Metal Resistance
merT Tn5086 540-890 + Passenger Gene Heavy Metal Resistance
merP Tn5086 904-1179 + Passenger Gene Heavy Metal Resistance
merC Tn5086 1215-1637 + Passenger Gene Heavy Metal Resistance
merA Tn5086 1689-3383 + Passenger Gene Heavy Metal Resistance
merD Tn5086 3401-3763 + Passenger Gene Heavy Metal Resistance
merE Tn5086 3760-3996 + Passenger Gene Heavy Metal Resistance
urfM 5'-end Tn5086 3993-4663 + Passenger Gene Other
tniA In22 4775-6454 + Transposase
tniB delta1 In22 6457-7313 + Accessory Gene
GNAT_fam In22 7418-7918 - Passenger Gene Antibiotic Resistance
sul1 (ARO:3000410) In22 8046-8885 - Passenger Gene Antibiotic Resistance
qacEdelta1 (ARO:3005010) In22 8879-9226 - Passenger Gene Antibiotic Resistance
dfrA7 (ARO:3002862) In22 9456-9929 - Passenger Gene Antibiotic Resistance
intI1 In22 10087-11100 + Integron Integrase Class 1
tnpM Tn5086 11303-11653 + Accessory Gene Inhibitor
tnpR Tn5086 11779-12339 + Accessory Gene Resolvase
tnpA Tn5086 12342-15308 + Transposase
ORF Details
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
merR MerR Tn5086 144 34-468 -
Class: Passenger Gene
Subclass: Heavy Metal Resistance
Function: activator-repressor of mer operon
Target: Mercury
Protein Sequence: MENNLENLTI GVFAKAAGVN VETIRFYQRK GLLREPDKPY GSIRRYGEAD VVRVKFVKSA QRLGFSLDEI AELLRLDDGT HCEEASSLAE HKLKDVREKM ADLARMETVL SELVCACHAR KGNVSCPLIA SLQGEAGLAR SAMP

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
merT MerT Tn5086 116 540-890 +
Class: Passenger Gene
Subclass: Heavy Metal Resistance
Function: cytosolic mercuric ion transport protein
Target: Mercury
Protein Sequence: MSEPQNGRGA LFAGGLAAIL ASTCCLGPLV LVALGFSGAW IGNLTVLEPY RPLFIGAALV ALFFAWKRIY RPVQACKPGE VCAIPQVRAT YKLIFWIVAV LVLVALGFPY VVPFFY

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
merP MerP Tn5086 91 904-1179 +
Class: Passenger Gene
Subclass: Heavy Metal Resistance
Function: mercury transport
Target: Mercury
Protein Sequence: MKKLFASLAL AAAVAPVWAA TQTVTLAVPG MTCAACPITV KKALSKVEGV SKVDVGFEKR EAVVTFDDTK ASVQKLTKAT ADAGYPSSVK Q

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
merC MerC Tn5086 140 1215-1637 +
Class: Passenger Gene
Subclass: Heavy Metal Resistance
Function: transmembrane protein mercury transport
Target: Mercury
Protein Sequence: MGLMTRIADK TGALGSVVSA MGCAACFPAL ASFGAAIGLG FLSQYEGLFI SRLLPLFAAL AFLANALGWF SHRQWLRSLL GMIGPAIVFA ATVWLLGNWW TANLMYVGLA LMIGVSIWDF VSPAHRRCGP DGCELPAKRL

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
merA MerA Tn5086 564 1689-3383 +
Class: Passenger Gene
Subclass: Heavy Metal Resistance
Function: mercuric ion reductase
Target: Mercury
Protein Sequence: MSTLKITGMT CDSCAVHVKD ALEKVPGVQS ADVSYAKGSA KLAIEVGTSP DALTAAVAGL GYRATLADAP SVSTPGGLLD KMRDLLGRND KTGSSGALHI AVIGSGGAAM AAALKAVEQG ARVTLIERGT IGGTCVNVGC VPSKIMIRAA HIAHLRRESP FDGGIAATTP TIQRTALLAQ QQARVDELRH AKYEGILEGN PAITVLHGSA RFKDNRNLIV QLNDGGERVV AFDRCLIATG ASPAVPPIPG LKDTPYWTST EALVSETIPK RLAVIGSSVV ALELAQAFAR LGAKVTILAR STLFFREDPA IGEAVTAAFR MEGIEVREHT QASQVAYING EGDGEFVLTT AHGELRADKL LVATGRAPNT RKLALDATGV TLTPQGAIVI DPGMRTSVEH IYAAGDCTDQ PQFVYVAAAA GTRAAINMTG GDAALNLTAM PAVVFTDPQV ATVGYSEAEA HHDGIKTDSR TLTLDNVPRA LANFDTRGFI KLVVEEGSGR LIGVQAVAPE AGELIQTAAL AIRNRMTVQE LADQLFPYLT MVEGLKLAAQ TFNKDVKQLS CCAG

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
merD MerD Tn5086 120 3401-3763 +
Class: Passenger Gene
Subclass: Heavy Metal Resistance
Function: secondary regulatory protein
Target: Mercury
Protein Sequence: MSAYTVSQLA HNAGVSVHIV RDYLVRGLLR PVACTTGGYG VFDDAALQRL CFVRAAFEAG IGLDALARLC RALDAADGAQ AAAQLAVLRQ LVERRRAALA HLDAQLASMP AERAHEEALP

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
merE MerE Tn5086 78 3760-3996 +
Class: Passenger Gene
Subclass: Heavy Metal Resistance
Function: mercury transport
Target: Mercury
Comment: similar to urf-1 in pKLH2 (GenBank AF213017), pKLH272 (Genbank Y08992), pMER610 (GenBank Y08993), pKLH210 (GenBank Y10102), Tn5036 (Genbank Y09025), orf1 in Tn501 (GenBank Z00027), and urf-1 in Tn5041 (GenBank X98999)
Protein Sequence: MNAPDKLPPE TRQPVSGYLW GALAVLTCPC HLPILAAVLA GTTAGAFLGE HWGVAALALT GLFVLAVTRL LRAFRGGS

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
urfM 5'-end N/A Tn5086 223 3993-4663 +
Class: Passenger Gene
Subclass: Other
Comment: urfMORF interrupted by insertion of In2
Protein Sequence: MTSSQPAGWT AAELAQAAAR GQLDLHYQPL VDLRDHRIAG AEALMRWRHP RLGLLPPGQF LPLAESFGLM PEIGAWVLGE ACRQMHKWQG PAWQPFRLAI NVSASQVGPT FDDEVKRVLA DMALPAELLE IELTESVAFG NPALFASFDA LRAIGVRFAA DDFGTGYSCL QHLKCCPITT LKIDQSFVAR LPDDARDQTI VRAVIQLAHG LGMDVIFRRR LHQ

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tniA TniA In22 559 4775-6454 +
Class: Transposase
Transposase Chemistry: DDE
Comment: can be extended upstream by 12 amino acids| identical to tniA (Tn1721 and In2)| 25% amino acid sequence identity to TnsB from Tn7
Protein Sequence: MATDTPRIPE QGVATLPDEA WERARRRAEI ISPLAQSETV GHEAADMAAQ ALGLSRRQVY VLIRRARQGS GLVTDLVPGQ SGGGKGKGRL PEPVERVIHE LLQKRFLTKQ KRSLAAFHRE VTQVCKAQKL RVPARNTVAL RIASLDPRKV IRRREGQDAA RDLQGVGGEP PAVTAPLEQV QIDHTVIDLI VVDDRDRQPI GRPYLTLAID VFTRCVLGMV VTLEAPSAVS VGLCLVHVAC DKRPWLEGLN VEMDWQMSGK PLLLYLDNAA EFKSEALRRG CEQHGIRLDY RPLGQPHYGG IVERIIGTAM QMIHDELPGT TFSNPDQRGD YDSENKAALT LRELERWLTL AVGTYHGSVH NGLLQPPAAR WAEAVARVGV PAVVTRATSF LVDFLPILRR TLTRTGFVID HIHYYADALK PWIARRERWP SFLIRRDPRD ISRIWVLEPE GQHYLEIPYR TLSHPAVTLW EQRQALAKLR QQGREQVDES ALFRMIGQMR EIVTSAQKAT RKARRDADRR QHLKTSARPD KPVPPDTDIA DPQADNLPPA KPFDQIEEW

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tniB delta1 TniB delta1 In22 285 6457-7313 +
Class: Accessory Gene
Function: probable ATP-binding protein.
Comment: probably truncated by insertion of IS1326::IS1353
Protein Sequence: MDEYPIIDLS HLLPAAQGLA RLPADERIQR LRADRWIGYP RAVEALNRLE ALYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRTHPASSD ADQEHIPVLV VQMPSEPSVI RFYVALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRD AYLAIRSDDQ LENRFEPMML PVWEANDDCC SLLASFAASL PLRRPSPIAT LDMARYLLTR SEGTIGELAH LLMAAAIVAV ESGEEAINHR TLSMA

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
GNAT_fam GNAT_fam In22 166 7418-7918 -
Class: Passenger Gene
Subclass: Antibiotic Resistance
Sequence Family: GNAT
Target: aminoglycoside antibiotic (ARO:0000016)
Protein Sequence: MDSEEPPNVR VACSGDIDEV VRLMHDAAAW MSAKGTPAWD VARIDRTFAE TFVLRSELLV ASCSDGIVGC CTLSAEDPEF WPDALKGEAA YLHKLAVRRT HAGRGVSSAL IEACRHAART QGCAKLRLDC HPNLRGLYER LGFTHVDTFN PGWDPTFIAE RLELEI

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
sul1 (ARO:3000410) Sul1 In22 279 8046-8885 -
Class: Passenger Gene
Subclass: Antibiotic Resistance
Function: antibiotic target replacement (ARO:0001002)
Sequence Family: sulfonamide resistant sul (ARO:3004238)
Transposase Chemistry: dihydropteroate synthase
Target: sulfonamide antibiotic (ARO:3000282)||sulfone antibiotic (ARO:3003401)
Comment: perfect match to reference sequence for ARO:3000410
Protein Sequence: MVTVFGILNL TEDSFFDESR RLDPAGAVTA AIEMLRVGSD VVDVGPAASH PDARPVSPAD EIRRIAPLLD ALSDQMHRVS IDSFQPETQR YALKRGVGYL NDIQGFPDPA LYPDIAEADC RLVVMHSAQR DGIATRTGHL RPEDALDEIV RFFEARVSAL RRSGVAADRL ILDPGMGFFL SPAPETSLHV LSNLQKLKSA LGLPLLVSVS RKSFLGATVG LPVKDLGPAS LAAELHAIGN GADYVRTHAP GDLRSAITFS ETLAKFRSRD ARDRGLDHA

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
qacEdelta1 (ARO:3005010) QacEdelta1 In22 115 8879-9226 -
Class: Passenger Gene
Subclass: Antibiotic Resistance
Function: antibiotic efflux (ARO:0010000)
Sequence Family: major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002)
Target: disinfecting agents and antiseptics(ARO:3005386)
Comment: subunit of the qac multidrug efflux pump||perfect match to reference sequence for ARO:3005010 (bitscore:219)
Protein Sequence: MKGWLFLVIA IVGEVIATSA LKSSEGFTKL APSAVVIIGY GIAFYFLSLV LKSIPVGVAY AVWSGLGVVI ITAIAWLLHG QKLDAWGFVG MGLIIAAFLL ARSPSWKSLR RPTPW

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
dfrA7 (ARO:3002862) DfrA7 In22 157 9456-9929 -
Class: Passenger Gene
Subclass: Antibiotic Resistance
Function: antibiotic target replacement (ARO:0001002)
Sequence Family: trimethoprim resistant dihydrofolate reductase dfr (ARO:3001218)
Target: diaminopyrimidine antibiotic (ARO:3000171)
Comment: 100% identity to reference sequence ARO:3002862 in Acinetobacter baumannii (bitscore: 319)
Protein Sequence: MKISLISATS ENGVIGNGPD IPWSAKGEQL LFKALTYNQW LLVGRKTFDS MGVLPNRKYA VVSRKGISSS NENVLVFPSI EIALQELSKI TDHLYVSGGG QIYNSLIEKA DIIHLSTVHV EVEGDINFPK IPENFNLVFE QFFLSNINYT YQIWKKG

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
intI1 IntI1 In22 337 10087-11100 +
Class: Integron Integrase
Subclass: Class 1
Sequence Family: Class 1 Integron Tyrosine Integrase
Transposase Chemistry: Tyrosine
Protein Sequence: MKTATAPLPP LRSVKVLDQL RERIRYLHYS LRTEQAYVHW VRAFIRFHGV RHPATLGSSE VEAFLSWLAN ERKVSVSTHR QALAALLFFY GKVLCTDLPW LQEIGRPRPS RRLPVVLTPD EVVRILGFLE GEHRLFAQLL YGTGMRISEG LQLRVKDLDF DHGTIIVREG KGSKDRALML PESLAPSLRE QLSRARAWWL KDQAEGRSGV ALPDALERKY PRAGHSWPWF WVFAQHTHST DPRSGVVRRH HMYDQTFQRA FKRAVEQAGI TKPATPHTLR HSFATALLRS GYDIRTVQDL LGHSDVSTTM IYTHVLKVGG AGVRSPLDAL PPLTSER

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpM TnpM Tn5086 116 11303-11653 +
Class: Accessory Gene
Subclass: Inhibitor
Function: transposition regulator||reported to enhance Tn21 transposition and suppress resolution of cointegrate replicons in vivo
Comment: 3'-end of urfM ORF, which is interrupted by insertion of In2||inhibits tranposition probably by inhibiting resolution
Protein Sequence: MEVVAEGVET PDCLAWLRQA GCDTVQGFLF ARPMPAAAFV GFVNQWRNTT MNANEPSTSC CVCCKEIPLD AAFTPEGAEY VEHFCGLECY QRFQARASTA TETSVKPDAC DSPPSG

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpR TnpR Tn5086 186 11779-12339 +
Class: Accessory Gene
Subclass: Resolvase
Function: resolvase||serine site-specific recombinase
Sequence Family: Serine Site-Specific Recombinase
Transposase Chemistry: Serine
Comment: identical to tnpR (TnAs3 )
Protein Sequence: MTGQRIGYIR VSTFDQNPER QLEGVKVDRA FSDKASGKDV KRPQLEALIS FARTGDTVVV HSMDRLARNL DDLRRIVQTL TQRGVHIEFV KEHLSFTGED SPMANLMLSV MGAFAEFERA LIRERQREGI ALAKQRGAYR GRKKSLSSER IAELRQRVEA GEQKTKLARE FGISRETLYQ YLRTDQ

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpA TnpA Tn5086 988 12342-15308 +
Class: Transposase
Function: transposition, DNA-mediated (GO:0006313)
Transposase Chemistry: DDE
Comment: identical to TnAs3 tnpA
Protein Sequence: MPRRSILSAA ERESLLALPD SKDDLIRHYT FNDTDLSIIR QRRGPANRLG FAVQLCYLRF PGVILGVDEL PFPPLLKLVA DQLKVGVESW NEYGQREQTR REHLSELQTV FGFRPFTMSH YRQAVQMLTE LAMQTDKGIV LASALIGHLR RQSVILPALN AVERASAEAI TRANRRIYDA LAEPLADAHR RRLDDLLKRR DNGKTTWLAW LRQSPAKPNS RHMLEHIERL KAWQALDLPT GIERLVHQNR LLKIAREGGQ MTPADLAKFE PQRRYATLVA LATEGMATVT DEIIDLHDRI LGKLFNAAKN KHQQQFQASG KAINAKVRLY GRIGQALIDA KQSGRDAFAA IEAVMSWDSF AESVTEAQKL AQPDDFDFLH RIGESYATLR RYAPEFLAVL KLRAAPAAKN VLDAIEVLRG MNTDNARKLP ADAPTGFIKP RWQKLVMTDA GIDRRYYELC ALSELKNSLR SGDIWVQGSR QFKDFEDYLV PPEKFTSLKQ SSELPLAVAT DCEQYLHERL TLLEAQLATV NRMAAANDLP DAIITESGLK ITPLDAAVPD TAQALIDQTA MVLPHVKITE LLLEVDEWTG FTRHFTHLKS GDLAKDKNLL LTTILADAIN LGLTKMAESC PGTTYAKLAW LQAWHTRDET YSTALAELVN AQFRHPFAGH WGDGTTSSSD GQNFRTASKA KSTGHINPKY GSSPGRTFYT HISDQYAPFH TKVVNVGLRD STYVLDGLLY HESDLRIEEH YTDTAGFTDH VFALMHLLGF RFAPRIRDLG DTKLYIPKGD AAYDALKPMI GGTLNIKHVR AHWDEILRLA TSIKQGTVTA SLMLRKLGSY PRQNGLAVAL RELGRIERTL FILDWLQSVE LRRRVHAGLN KGEARNALAR AVFFNRLGEI RDRSFEQQRY RASGLNLVTA AIVLWNTVYL ERAAHALRGN GHAVDDSLLQ YLSPLGWEHI NLTGDYLWRS SAKIGAGKFR PLRPLQPA

TnCentral Accession TE Name Type Coordinates Strand Length
In22-CP054343 In22 integron 4634-11302 + 6669

Name Associated TE Coordinates Direction Length Sequence
IRt In22 4634-4666 + 33 TGTCATTTTC AGAAGACGAC TGCACCAGTT GAT
repeat t1 In22 4642-4660 + 19 TCAGAAGACG ACTGCACCA
repeat t2 In22 4682-4700 - 19 TCAGGAGCTG GCTGCACAA
repeat t3 In22 4711-4730 + 20 TCAGAAGTGA TCTGCACCAA
repeat t4 In22 4743-4761 + 19 TCAATACTCG TGTGCACCA
repeat i4 In22 11183-11201 - 19 TCAGCGGACG CAGGGAGGA
repeat i3 In22 11211-11229 - 19 TCAGGCAACG ACGGGCTGC
repeat i2 In22 11253-11271 - 19 TCAGAAGCCG ACTGCACTA
IRi In22 11270-11302 - 33 TGTCGTTTTC AGAAGACGGC TGCACTGAAC GTC
repeat i1 In22 11276-11294 - 19 TCAGAAGACG GCTGCACTG