Mobile element type:
Transposon
Name:
Tn5058
Synonyms:
Accession:
Tn5058-Y17897
Family:
Tn402
Group:
Tn5053
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Pseudomonas sp. ED23-33
Date of Isolation:
2005
Country:
Russia
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRt | 1-25 | + | 25 | TGTCATTTTC AGAAGACGAC TGCAC |
| repeat t1 | 9-27 | + | 19 | TCAGAAGACG ACTGCACCA |
| repeat t2 | 49-67 | - | 19 | TCAGGAGTCG CCTGCACAT |
| repeat t4 | 110-128 | + | 19 | TCAATACTCG TGTGCACCA |
| IR Tn21-like | 12273-12310 | - | 38 | GTGGTCGTCT CAGAAAACGG AAAATAAAGC ACGCTAAG |
| repeat i4 | 12283-12301 | - | 19 | TCAGAAAACG GAAAATAAA |
| repeat i1 | 12347-12365 | - | 19 | TCAGAAGGTG ACTGCACGG |
| IRi | 12349-12373 | - | 25 | TGTCATTTTC AGAAGGTGAC TGCAC |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| repeat t3 | Tn5058 | 78-97 | + | 20 | TCAGAAGTCA TCTGCACCAA |
| r5 | Tn5058 | 3875-3888 | + | 14 | AATTTCTGTC ACAT |
| r3 | Tn5058 | 3931-3944 | + | 14 | GGGTTAAGTG ACAA |
| res | Tn5058 | 3975-4004 | + | 30 | CTGTCACATA ATCGAACGTA TATGTGACAG |
| r2 | Tn5058 | 3975-3988 | - | 14 | CGATTATGTG ACAG |
| r1 | Tn5058 | 3991-4004 | + | 14 | CGTATATGTG ACAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tniA | Tn5058 | 144-1826 | + | Transposase | |
| tniB | Tn5058 | 1829-2737 | + | Accessory Gene | |
| tniQ | Tn5058 | 2734-3951 | + | Accessory Gene | Target Site Selection |
| tniR | Tn5058 | 4012-4626 | + | Accessory Gene | Resolvase |
| merE | Tn5058 | 5003-5239 | - | Passenger Gene | Heavy Metal Resistance |
| merD2 | Tn5058 | 5236-5601 | - | Passenger Gene | Heavy Metal Resistance |
| merB2 | Tn5058 | 5712-6350 | - | Passenger Gene | Heavy Metal Resistance |
| merR | Tn5058 | 6419-6853 | + | Passenger Gene | Heavy Metal Resistance |
| merD1 | Tn5058 | 7486-7832 | - | Passenger Gene | Heavy Metal Resistance |
| merB1 | Tn5058 | 7829-8467 | - | Passenger Gene | Heavy Metal Resistance |
| merG | Tn5058 | 8681-9199 | - | Passenger Gene | Heavy Metal Resistance |
| merA | Tn5058 | 9235-10944 | - | Passenger Gene | Heavy Metal Resistance |
| merP | Tn5058 | 11132-11407 | - | Passenger Gene | Heavy Metal Resistance |
| merT | Tn5058 | 11421-11771 | - | Passenger Gene | Heavy Metal Resistance |
| merR1 | Tn5058 | 11843-12277 | + | Passenger Gene | Heavy Metal Resistance |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniA | TniA | Tn5058 | 560 | 144-1826 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | homologous to TnsB of Tn7 | ||||||||||
| Protein Sequence: | MASDTSLIAE QGVATLPDAA WAQARQRAEI IGPLAALDVV GHEAADAAAH ALGLSRRQVY VLIRRARQGA GLVTDLARSR SGGGKGKGRL PESVERIIRE LLQKRFLTKQ KRSLAAFHRE VAQACKAQKL RAPARNTVAL RIAGLDPLKA TRRREGQDAS RSLQGVGGEP PAVTAPLEQV QIDHTVIDLI VVDERDRQPI GRPYLTIAID VFTRCVLGMV VTLEAPSSVS VGLCLVHVAC DKRPWLEGLK IEMEWPMSGK PRLLYLDNAA EFKSEALRRG CEQHGIRLDY RPLGQPHYGG IVERIIGTAM QMIHDELPGT TFSNPDQRGD YDSENKAALT LRELERWLTL AVGTYHGSVH NGLLQPPAAR WAEAIARTGV PTVITRTTAF LVDFLPIIRR TLTRTGFVID HIHYYADALK PWIARRDRLP AFLIRRDPRD ISRIWVLEPE GQHYLEIPYR TLSHPAVTLW EQRQALAKLR QQGREQVDES ALFRMIGQMR EIVSTAQKAT RKARRDADRR QHLKATAVLF KTTPPPDADM ADPQADNQPP AKPFDQIEEW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniB | TniB | Tn5058 | 302 | 1829-2737 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | homologous to TnsC protein of Tn7 putative ATP-binding protein | ||||||||||
| Protein Sequence: | VEEYPIIDLS HLMPVAQGLA RLPADERIHR LRADRWIGYP RAVEALNRLE ALYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRTHPASSD ADQEHIPVLV VQMPSEPSVI RFYVALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRD AYLAIRSDDQ LENRFEPMML PVWEANDDCC SLLASFAASL PLRRPSSIAT LDMARYLLTR SEGTIGELAH LLMAAAVAAV ESGEEAINHR TLSMADYTGP SERRRQFERE LM |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniQ | TniQ | Tn5058 | 405 | 2734-3951 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Target Site Selection | ||||||||||
| Function: | putative transposition gene | ||||||||||
| Comment: | similar function to Tn7 tnsD? | ||||||||||
| Protein Sequence: | MKPAPHWPLH PAPREGEALS SWLNRVALCY HMEVSELLEH DLGHGQVDDL DTAPPLALLA MLSQRSGIEP DRLRCMSFAG WVPWLLDSLD DQIPDALETY AFQLSVLLPK LRRRTRSITS WRAWLPSQPI HRACPLCLND PENQAVLLAW KLPLMLSCPL HGCWLESYWG VPGRFLGWEN ADAEPRTASD AIAAMDQRTW QALTTGHVEL PRRRIHAALW FRLLRTLLDE LNTPLSTCGT YAGYLRQIWE CCGHPLRAGQ SLWRPYETLN PAVRLQMLEA AATAISLIEV RDISPPGEHA KLFWSEPQTG FTSGLPAKAP KPEPVDHWQR AIQAIDEAII EARHNPETAR SLFALASYGR RDPASLEQLR ATFAKEGIPP EFLSHYEPSL PFACLRQNDG LSDKF |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniR | TniR | Tn5058 | 204 | 4012-4626 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | putative resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | resolution of cointegrates || Protein: ACE81792.1 | ||||||||||
| Protein Sequence: | MLIGYMRVSK ADGSQATDLQ RDALVAAGVD PAHLYEDQAS GKREDRPGLA SCLKALRPGD TLVVWKLDRL GRDLRNLINT VHDLTERGIG LKVLTGHVAA IDTTTAAGKL VFGIFAALAE FERELIAERT VAGLASAQTR GRKGGRPFKM TAAKLRLAMA AMGQSQTKVG DLCQELGITR QTLYRHISPK GELRPDGEKL LSRI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merE | MerE | Tn5058 | 78 | 5003-5239 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercury transport | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | similar to urf-1 in pKLH2 (GenBank AF213017), pKLH272 (Genbank Y08992), pMER610 (GenBank Y08993), pKLH210 (GenBank Y10102), Tn5036 (Genbank Y09025), orf1 in Tn501 (GenBank Z00027), and urf-1 in Tn5041 (GenBank X98999) | ||||||||||
| Protein Sequence: | MNSPERIPAE THKPFTGYLW GTLAVLTCPC HLSILAAVLA GTTAGAFLVE YWVITALGLT GLFLLSLARA LRAFRERS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD2 | MerD2 | Tn5058 | 121 | 5236-5601 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MNAYTVSRLA LDAGVSVHIV RDYLLRGLLR PVACTTGGYG LFDDAALQRL CFVRAAFEAG IGLGALARLC RALDAANCDE TAAQLAVLRQ FVERRREALA NLEVQLAAMP TAPAQHAESL P |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merB2 | MerB2 | Tn5058 | 212 | 5712-6350 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | organomercurial lyase | ||||||||||
| Protein Sequence: | MKLAPYILER LTPTNRPKGF AEFLVALLGE LAKGRPISRT TLARTLGWPA EQVAAVLEQA TSTEYDNDGN IIGYVLTLRE TSHVLEIDNR RLYAWCALDT LMLPALIGRT ARVSSHCAAT GAPVSLTVSP SEIQAVEPAG MAVSLVLPQE AADVRQSFCC HVHFFASVPT AEDWASKHQG LEGLAIVSVH EAFGLGQEFN RHLLQTMSSR TP |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR | MerR | Tn5058 | 144 | 6419-6853 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | putative regulatory gene mercury resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MENNLENLTI GVFAKAAGVN VETIRFYQRK GLLPEPDKPY GSIRRYGAAD VTRVRFVKSA QRLGFSLDEI AELLRLDDGT HCEEASSLAE HKLQDVREKM ADLARMEAVL SDLVCACHSR QGNVSCPLIA SLQGGTSLAG ASTA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD1 | MerD1 | Tn5058 | 115 | 7486-7832 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MNAYSISKLA DDACVQCMSC ATT*YAACCT LLGEPKVART FSMTKHSEGC GFSAQPSSPE LGSTNWRDFA KHWTQTIAKA WTDASSACDG RSTCVEQF*L SSRPL*MKWR PVQLL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merB1 | MerB1 | Tn5058 | 212 | 7829-8467 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | organomercurial lyase | ||||||||||
| Protein Sequence: | MDKTIYSKKI AESLSSGNHP KEFATLFVAL LRQLAMGGPV SREKLAGALG WSGARVATVL EQAPGTEYDD EANIIGLGLT LRETSHVFEV DGRHLYTWCA LDTLMFPALT GKIARVTSPC AATGRPITLT VAPEAVLHVE PAEAMVSLRT PDTSPDIRCS FCCHVHFFAS PSIANSWAST HQGIEVVPVE SAFDLGHDVA LKLLEDCEES PV |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merG | MerG | Tn5058 | 172 | 8681-9199 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MFCDIRLKLR RVRACLTIVL ASTATLGMPN QALAAYDFSK LGAAIETLSP ETVSDWEKKA RAGDAMAQNI MGLAYKCGMG VKQNHAASIQ WFAGRPSRVK PMHSSTSGAS TEVKSTVCIR MGVRLLPTMQ RLSSGTASLP SRDTRRLKSG WPSCLQRVLP TSRPTRCRHT SG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA | MerA | Tn5058 | 569 | 9235-10944 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MQELNEVGLS VAGMTCPSCT RHVEDALLAV PGVTRAAVDY PSNKAQVTGN RLDVSALVAA VGALGYGATP TDEFESKERS VEQPGLLGKA AQWLSGERAV EKPAGQLHVA IIGTGGAAVA AALKAAENGA RVTLIERGTI GGTCVNVGCV PSKIMIRAAH IAHLRRESPF DVGLSATPPV VLRDRLLAQQ QARVDELRHA KYESILESNP SINLVRGSAR FKDGQTLIVE AAEGDTREVA FDRCLIATGA SAAIPPLPGL AGTPYWTSTE ALVSEAIPKR LAVIGASVVA LELAQAFARL GSEVTVLARR TLFASDDPAI GEAVTAAFRA EGITVLTQTQ ASAVTYCDRQ FILTTAQGEL KVDQLLVATG RTPNTASLNL ELAGVTFDSQ HRILIDQGMR TSASNIYAAG DCTDQPQYVY VAAAAGTRAG INMTGGDVKL DLDAMPAVVF TDPQVATVGY SEAEAQRIGL QTESRTLTLD NVPRALANFD TRGFIKLVAE AGSGRILVVQ AVTPEAGEII QTAAIAIRAR MTVQDLANQL FPYLTMVEGL KLVAQTFSKD VTQLSCCAG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | Tn5058 | 91 | 11132-11407 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | periplasmic mercuric ion binding protein | ||||||||||
| Protein Sequence: | MKKLFASLAL AAAVAPVWAA TQTVTLAVPG MTCATCPITI KKALSKVDGV SKTEVDFSTK LAVVTFDDAK TNVQALSKAT TDVGYPSELK K |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | Tn5058 | 116 | 11421-11771 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | cytosolic mercuric ion transport protein | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MSEPQNGRGA LFAGGLAAIL ASTCCLGPLV LVALGFSGAW IGNLTVLEPY RPLFIGAALV ALFFAWKRIY RPVQACKPGE VCAIPQVRAT YKLIFWIVAV LVLVALGFPY VVPFFY |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR1 | MerR1 | Tn5058 | 144 | 11843-12277 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | activator/repressor of mer operon | ||||||||||
| Protein Sequence: | MENNLENLTI GVFAKAAGVN VETIRFYQRK GLLPEPDKPY GSIRRYGEAD VVRVKFVKSA QRLGFSLDEI AELLRLDDGT HCEEASSLAE HKLQDVREKM ADLARMEAVL SDLVCACHSR QGNVSCPLIA SLQGGTSLAG ASTA |
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1. Mindlin S, Minakhin L, Petrova M, Kholodii G, Minakhina S, Gorlenko Z, Nikiforov V.
Present-day mercury resistance transposons are common in bacteria preserved in permafrost grounds since the Upper Pleistocene.. Res Microbiol. 2005 Dec;156(10):994-1004. doi: 10.1016/j.resmic.2005.05.011. Epub 2005 Jul 18. PubMed ID:
16084067
.