Mobile element type:
Transposon
Name:
Tn5053.8
Synonyms:
Accession:
Tn5053.8-CP017991
Family:
Tn402
Group:
Tn5053
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Enterobacter cloacae complex sp. ECNIH7
Date of Isolation:
2016
Country:
U.s.a.
Molecular Source:
plasmid pENT-1ac
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRt | 1-25 | + | 25 | TGTCATTTTC AGAAGACGAC TGCAC |
| repeat t1 | 9-27 | + | 19 | TCAGAAGACG ACTGCACCA |
| repeat t2 | 49-67 | - | 19 | TCAGGAGTCG CCTGCACAT |
| repeat t4 | 110-128 | + | 19 | TCAATACTCG TGTGCACCA |
| repeat i4 | 7087-7105 | - | 19 | TCAGAAAACG GAAAATAAA |
| IR Tn21-like | 12505-12542 | - | 38 | GTGGTCGTCT CAGAAAACGG AAAATAAAGC ACGCTAAG |
| repeat i4 | 12515-12533 | - | 19 | TCAGAAAACG GAAAATAAA |
| repeat i1 | 12579-12597 | - | 19 | TCAGAAGGTG ACTGCACGG |
| IRi | 12581-12605 | - | 25 | TGTCATTTTC AGAAGGTGAC TGCAC |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| repeat t3 | Tn5053.8 | 78-97 | + | 20 | TCAGAAGTCA TCTGCACCAA |
| r5 | Tn5053.8 | 3875-3888 | + | 14 | AATTTCTGTC ACAT |
| r3 | Tn5053.8 | 3931-3944 | + | 14 | GGGTTAAGTG ACAA |
| res | Tn5053.8 | 3975-4004 | + | 30 | CTGTCACATA ATCGAACGTA TATGTGACAG |
| r2 | Tn5053.8 | 3975-3988 | - | 14 | CGATTATGTG ACAG |
| r1 | Tn5053.8 | 3991-4004 | + | 14 | CGTATATGTG ACAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tniA | Tn5053.8 | 144-1826 | + | Transposase | |
| tniB | Tn5053.8 | 1829-2737 | + | Accessory Gene | |
| tniQ | Tn5053.8 | 2734-3951 | + | Accessory Gene | Target Site Selection |
| tniR | Tn5053.8 | 4012-4626 | + | Accessory Gene | Resolvase |
| tniM | Tn5053.8 | 4665-4985 | - | Passenger Gene | Hypothetical |
| merE | Tn5053.8 | 5001-5237 | - | Passenger Gene | Heavy Metal Resistance |
| merD | Tn5053.8 | 5234-5695 | - | Passenger Gene | Heavy Metal Resistance |
| merB | Tn5053.8 | 5711-6349 | - | Passenger Gene | Heavy Metal Resistance |
| merA_p | Tn5053.8 | 6364-6690 | - | Passenger Gene | Heavy Metal Resistance |
| merR | Tn5053.8 | 6647-7081 | + | Passenger Gene | Heavy Metal Resistance |
| WP_048976769.1 | Tn5053.8 | 7181-7624 | + | Passenger Gene | Hypothetical |
| merD1 | Tn5053.8 | 7715-8062 | - | Passenger Gene | Heavy Metal Resistance |
| merB1 | Tn5053.8 | 8059-8697 | - | Passenger Gene | Heavy Metal Resistance |
| merG | Tn5053.8 | 8778-9431 | - | Passenger Gene | Heavy Metal Resistance |
| merA | Tn5053.8 | 9467-11176 | - | Passenger Gene | Heavy Metal Resistance |
| merP | Tn5053.8 | 11364-11639 | - | Passenger Gene | Heavy Metal Resistance |
| merT | Tn5053.8 | 11653-12003 | - | Passenger Gene | Heavy Metal Resistance |
| merR1 | Tn5053.8 | 12075-12509 | + | Passenger Gene | Heavy Metal Resistance |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniA | TniA | Tn5053.8 | 560 | 144-1826 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | homologous to TnsB of Tn7 | ||||||||||
| Protein Sequence: | MASDTSLIAE QGVATLPDAA WAQARQRAEI IGPLAALDVV GHEAADAAAH ALGLSRRQVY VLIRRARQGA GLVTDLARSR SGGGKGKGRL PESVERIIRE LLQKRFLTKQ KRSLAAFHRE VAQACKAQKL RAPARNTVAL RIAGLDPLKA TRRREGQDAS RSLQGVGGEP PAVTAPLEQV QIDHTVIDLI VVDERDRQPI GRPYLTIAID VFTRCVLGMV VTLEAPSSVS VGLCLVHVAC DKRPWLEGLN IEMEWPMSGK PRLLYLDNAA EFKSEALRRG CEQHGIRLDY RPLGQPHYGG IVERIIGTAM QMIHDELPGT TFSNPDQRGD YDSENKAALT LRELERWLTL AVGTYHGSVH NGLLQPPAAR WAEAIARTGV PTVITRTTAF LVDFLPIIRR TLTRTGFVID HIHYYADALK PWIARRDRLP AFLIRRDPRD ISRIWVLEPE GQHYLEIPYR TLSHPAVTLW EQRQALAKLR QQGREQVDES ALFRMIGQMR EIVSTAQKAT RKARRDADRR QHLKATAVLF KTTPPPDADM ADPQADNQPP AKPFDQIEEW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniB | TniB | Tn5053.8 | 302 | 1829-2737 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | homologous to TnsC protein of Tn7 putative ATP-binding protein | ||||||||||
| Protein Sequence: | MEEYPIIDLS HLMPVAQGLA RLPADERIHR LRADRWIGYP RAVEALNRLE ALYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRTHPASSD ADQEHIPVLV VQMPSEPSVI RFYVALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRD AYLAIRSDDQ LENRFEPMML PVWEANDDCC SLLASFAASL PLRRPSSIAT LDMARYLLTR SEGTIGELAH LLMAAAVAAV ESGEEAINHR TLSMADYTGP SERRRQFERE LM |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniQ | TniQ | Tn5053.8 | 405 | 2734-3951 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Target Site Selection | ||||||||||
| Function: | putative transposition gene | ||||||||||
| Comment: | similar function to Tn7 tnsD? | ||||||||||
| Protein Sequence: | MKPAPHWPLH PAPREGEALS SWLNRVALCY HMEVSELLEH DLGHGQVDDL DTAPPLALLA MLSQRSGIEP DRLRCMSFAG WVPWLLDSLD DQIPDALETY AFQLSVLLPK LRRRTRSITS WRAWLPSQPI HRACPLCLND PENQAVLLAW KLPLMLSCPL HGCWLESYWG VPGRFLGWEN ADAEPRTASD AIAAMDQRTW QALTTGHVEL PRRRIHAGLW FRLLRTLLDE LNTPLSTCGT YAGYLRQIWE CCGHPLRAGQ SLWRPYETLN PAVRLQMLEA AATAISLIEV RDISPPGEHA KLFWSEPQTG FTSGLPAKAP KPEPVDHWQR AIQAIDEAII EARHNPETAR SLFALASYGR RDPASLEQLR ATFAKEGIPP EFLSHYEPSL PFACLRQNDG LSDKF |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniR | TniR | Tn5053.8 | 204 | 4012-4626 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | putative resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | resolution of cointegrates || Protein: ACE81792.1 | ||||||||||
| Protein Sequence: | MLIGYMRVSK ADGSQATDLQ RDALVAAGVD PAHLYEDQAS GKREDRPGLA SCLKALRPGD TLVVWKLDRL GRDLRHLINT VHDLTGRGIG LKVLTGHGAA IDTTTAAGKL VFGIFAALAE FERELIAERT VAGLASARAR GRKGGRPFKM TAAKLRLAMA AMGQSETKVG DLCQELGITR QTLYRHISPK GELRPDGEKL LSRI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniM | TniM | Tn5053.8 | 106 | 4665-4985 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Function: | putative modulator protein of transposition | ||||||||||
| Protein Sequence: | MDGLRRRAQA FERGQGFLFA KPMPAAAFAV FVSQWRGATM NANDPTATSC CVCCKEIPLD AAFTPEGAEY VEHFCGLECY QRFQARASTA TETSGEPNAC GSPPSN |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merE | MerE | Tn5053.8 | 78 | 5001-5237 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercury transport | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | similar to urf-1 in pKLH2 (GenBank AF213017), pKLH272 (Genbank Y08992), pMER610 (GenBank Y08993), pKLH210 (GenBank Y10102), Tn5036 (Genbank Y09025),orf1 in Tn501 (GenBank Z00027), and urf-1 in Tn5041 (GenBank X98999) | ||||||||||
| Protein Sequence: | MNSPERMPAE THKPFTGYLW GTLAVLTCPC HLSILAAVLA GTTAGAFLVE YWVITALGLT GLFLLSLARA LRAFRERS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD | MerD | Tn5053.8 | 153 | 5234-5695 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MFYGTGIGFA ARGLNSAKRY MHCREPTKRR CSMNAYTVSR LALDAGVSVH IVRDYLLRGL LRPVACTTGG YGLFDDAALQ RLCFVRAAFE AGIGLGALAR LCRALDAANC DETAAQLAVL RQFVERRREA LANLEVQLAA MPTAPAQHAE SLP |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merB | MerB | Tn5053.8 | 212 | 5711-6349 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | putative organomercurial lyase | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | organomercurial lyase | ||||||||||
| Protein Sequence: | MKLAPYILER LTSVNRTNGT ADLLVPLLRE LAKGRPVSRT TLAGILDWPA ERVAAVLEQA TSTEYDKDGN IIGYGLTLRE TSYVFEIDDR RLYAWCALDT LIFPALIGRT ARVSSHCAAT GAPVSLTVSP SEIQAVEPAG MAVSLVLPQE AADVRQSFCC HVHFFASVPT AEDWASKHQG LEGLAIVSVH EAFGLGQEFN RHLLQTMSSR TP |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA_p | MerA_p | Tn5053.8 | 108 | 6364-6690 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MAKTPMVRFS KLFSIPLDSV HEYGSKVTLS NSNSKGQAEE GSGRLIGVQA VAPEAGELIQ TAVLAIRNRM TVQELADQLF PYLTMVEGLK LAAQTFTKDV KQLSCCAG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR | MerR | Tn5053.8 | 144 | 6647-7081 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | putative regulatory gene mercury resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MENNLENLTI GVFAKAAGVN VETIRFYQRK GLLPEPDKPY GSIRRYGAAD VTRVRFVKSA QRLGFSLDEI AELLRLDDGT HCEEASSLAE HKLQDVREKM ADLARMEAVL SDLVCACHSR QGNVSCPLIA SLQGGTSLAG ASTA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | WP_048976769.1 | WP_048976769.1 | Tn5053.8 | 147 | 7181-7624 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Protein Sequence: | MKDQIQDLQN RLIQAESRAS ELIDTNAQLQ RQTHELEQAN AMHKSTLDRQ VADIQNLEHQ LNDASAKNRE RKHESKQLAD NIAALASQKA DADKQVAVLS QALEVTKTDL KASQNRAEVL SDENKLILQE KAVIQGQFKQ LQESLSR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD1 | MerD1 | Tn5053.8 | 115 | 7715-8062 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MNAYSISKLA DDACVSVHVV RDYMIRGLLH PARRTESGYN IFDDKTLGRL RFLRAAFESG IGLDELARLC KALDADDSES LDRCVERVRW KIDVRRTVLT VVEASLNEMA AGAAT |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merB1 | MerB1 | Tn5053.8 | 212 | 8059-8697 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | organomercurial lyase | ||||||||||
| Protein Sequence: | MNKTIYSKKI AESLSSGNHP KEFATLFVAL LRQLAMGGPV SREKLAGALG WSGARVATVL EQAPGTEYDD EANIIGLGLT LRETSHVFEV DGRHLYTWCA LDTLMFPALT GKIARVTSRC AATGRPITLT VAPEAVLHVE PAEAMVSLRT PDTSPDIRCS FCCHVHFFAS PSIANSWAST HQGIEVVPVE SAFDLGHDVA LKLLEDCEES PV |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merG | MerG | Tn5053.8 | 217 | 8778-9431 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MFCDIRLKLR RVRACLTIVL ASTATLGMPN QALAAYDFSK LGAAIETLSP ETVSDWEKKA RAGDAMAQNI MGLAYKCGMG VKQNHAASIQ WFRRAAEQGE ADAQFNLGRL YGSEVDGMYK NGRAAPANDA EAFKWYRLAA EQGHTQAQVR LAQLFAKGLT DIAPDQVQAY QWMSLAAASG EPTAAKVVAD YAAQMKPEQL QQAQLLAEEW QRRQGTK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA | MerA | Tn5053.8 | 569 | 9467-11176 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MQELNEVGLS VAGMTCPSCT RHVEDALLAV PGVTRAAVDY PSNKAQVTGN RLDVSALVAA VGALGYGATP TDEFESKERS VEQPGLLGKA AQWLSGERAV EKPAGQLHVA IIGTGGAAVA AALKAAENGA RVTLIERGTI GGTCVNVGCV PSKIMIRAAH IAHLRRESPF DVGLSATPPV VLRDRLLAQQ QARVDELRHA KYESILESNP SINLVRGSAR FKDGQTLIVE AAEGDTREVA FDRCLIATGA SAAIPPLPGL AGTPYWTSTE ALVSEAIPKR LAVIGASVVA LELAQAFARL GSEVTVLARR TLFASDDPAI GEAVTAAFRA EGITVLTQTQ ASAVTYCDRQ FILTTAQGEL KVDQLLVATG RTPNTASLNL ERAGVTFDSQ HRILIDQGMR TSASNIYAAG DCTDQPQYVY VAAAAGTRAG INMTGGDVKL DLDAMPAVVF TDPQVATVGY SEAEAQRIGL QTESRTLTLD NVPRALANFD TRGFIKLVAE AGSGRILGVQ AVTPEAGEII QTAAIAIRAR MTVQDLANQL FPYLTMVEGL KLVAQTFSKD VTQLSCCAG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | Tn5053.8 | 91 | 11364-11639 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | periplasmic mercuric ion binding protein | ||||||||||
| Protein Sequence: | MKKLFASLAL AAAVAPVWAA TQTVTLAVPG MTCATCPITI KKALSKVDGV SKTEVDFSTK LAVVTFDDAK TNVQALSKAT TDVGYPSELK K |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | Tn5053.8 | 116 | 11653-12003 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | cytosolic mercuric ion transport protein | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MSEPQNGRGA LFAGGLAAIL ASTCCLGPLV LVALGFSGAW IGNLTVLEPY RPLFIGAALV ALFFAWKRIY RPVQACKPGE VCAIPQVRAT YKLIFWIVAV LVLVALGFPY VVPFFY |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR1 | MerR1 | Tn5053.8 | 144 | 12075-12509 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | activator/repressor of mer operon | ||||||||||
| Protein Sequence: | MENNLENLTI GVFAKAAGVN VETIRFYQRK GLLPEPDKPY GSIRRYGEAD VVRVKFVKSA QRLGFSLDEI AELLRLDDGT HCEEASSLAE HKLQDVREKM ADLARMEAVL SDLVCACHSR QGNVSCPLIA SLQGGTSLAG ASTA |
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