Mobile element type:
Transposon
Name:
Tn5053.4
Synonyms:
Accession:
Tn5053.4-CP001919
Family:
Tn402
Group:
Tn5053
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Enterobacter cloacae subsp. cloacae ATCC 13047
Date of Isolation:
2010
Country:
China
Molecular Source:
plasmid pECL_A
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRt | 1-25 | + | 25 | TGTCGTTTTC AGAAGACGAC CGCAC |
| repeat t1 | 9-27 | + | 19 | TCAGAAGACG ACCGCACCA |
| repeat t2 | 49-67 | - | 19 | TCAGGAGCCG TATGCACAC |
| repeat t3 | 78-96 | + | 19 | TCAGGAGTCG TCTGCACCA |
| IR | 8346-8383 | - | 38 | GGAGGGAGTG ACCATCTCAT TAAATAAAGC ACGCTAAG |
| repeat i2 | 9725-9743 | + | 19 | CCGTGCGGTC GACTTTTGA |
| IRi | 9747-9774 | - | 28 | TGTCACTTTC AGAAGGCGAC AGCACGAA |
| repeat i1 | 9748-9766 | - | 19 | TCAGAAGGCG ACAGCACGA |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| r6 | Tn5053.4 | 3826-3840 | - | 15 | GCGCAAACGT TCCAG |
| r5 | Tn5053.4 | 3872-3885 | + | 14 | AGTTTTTGTC ACAT |
| r4 | Tn5053.4 | 3877-3890 | - | 14 | TCGTAATGTG ACAA |
| r3 | Tn5053.4 | 3928-3941 | + | 14 | GGGTTAAGTG ACAG |
| res | Tn5053.4 | 3969-4003 | + | 35 | ACACTGTCAC ATAATCGAAC GTATATGTGA CAGGT |
| r2 | Tn5053.4 | 3972-3985 | - | 14 | CGATTATGTG ACAG |
| r1 | Tn5053.4 | 3988-4001 | + | 14 | CGTATATGTG ACAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tniA | Tn5053.4 | 144-1823 | + | Transposase | |
| tniB | Tn5053.4 | 1826-2734 | + | Accessory Gene | |
| tniQ | Tn5053.4 | 2731-3948 | + | Accessory Gene | Target Site Selection |
| tniR | Tn5053.4 | 4009-4623 | + | Accessory Gene | Resolvase |
| merE | Tn5053.4 | 4676-4912 | - | Passenger Gene | Heavy Metal Resistance |
| merD | Tn5053.4 | 4909-5274 | - | Passenger Gene | Heavy Metal Resistance |
| merA | Tn5053.4 | 5291-6937 | - | Passenger Gene | Heavy Metal Resistance |
| merF | Tn5053.4 | 6934-7179 | - | Passenger Gene | Heavy Metal Resistance |
| merP | Tn5053.4 | 7182-7457 | - | Passenger Gene | Heavy Metal Resistance |
| merT | Tn5053.4 | 7473-7823 | - | Passenger Gene | Heavy Metal Resistance |
| merR | Tn5053.4 | 7895-8329 | + | Passenger Gene | Heavy Metal Resistance |
| tnp | IS4321 | 8429-9433 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniA | TniA | Tn5053.4 | 559 | 144-1823 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MTSDTPPIAA QGVATLPDEA WAQARHRTEI IGPLAALEVV GHEAADEAAQ ALGLSRRQVY VLIRRARQGT GLVTDLTPGR SGGGKGKGRL PEPVERIIRE LLQKRFLTKQ KRSLAAFHRE VAQACKTQKL PVPARNTVAQ RIAGLHPAKI ARSRGGQDAA RPLQGAGGIP PEVTMPLEQV QIDHTVIDLI VVDERDRQPI GRPYLTLAID VFTRCVLGMV VTLEAPSAVS VGLCLAHAAC DKRPWLEGLN VEMDWPMSGK PRLLYLDNAA EFKSEALRRG CEQHGIRLDY RPPGQPHYGG IVERIIGTAM QMIHDELPGT TFSNPGQRGE YDSEKMATLT LRELERWLAL AVGTYHGSVH NGLLQPPAAR WAEAVERVGV PAVVTRPTAF LVDFLPVIRR TLTRTGFVID HIHYYADALK PWIARRERLP AFLIRRDPRD ISRIWVLEPE GQHYLEIHYR TLSHPAVTLW EQRQALAKLR QLGREQVDES ALFRMIGQMR EIVTTAQKAT RKARRDADRR QHLKTSEPPA KPIPPDVDMA DPQADNLPPA KPFDQIEEW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniB | TniB | Tn5053.4 | 302 | 1826-2734 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Function: | is needed for transposition of Tn5053 | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | homologous to TnsC protein of Tn7 putative ATP-binding protein | ||||||||||
| Protein Sequence: | MDEYPVIDLS HLLPAAQGLA RLPADERIQR IRADRWIGYP RAVEALNRLE TLYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRAHPVGTD ADQEHIPVLV VQMPSEPSVI RFYVALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRE AYLAIRSDDQ LENRFEPMLL PPWEANEDCC SLLASFAASL PLRRPSPIAT LDMARYLLTR SEGTIGELAH LLVAAAVAAV ESGEEAINHR TLSMADYIGP SERRRQFERE LM |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniQ | TniQ | Tn5053.4 | 405 | 2731-3948 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Target Site Selection | ||||||||||
| Function: | is needed for transposition of Tn5053 | ||||||||||
| Protein Sequence: | MKSAPRWPLH PAPKEGEALS SWLNRVAACY QMDVHELLAH DLGHSQLDDL DTAPSLSLLT ALCQRSGVEL ERLRSMSLAG WVPWLLDSLD DSVPAALETY TFQCAVLLPK RTRKVRSITR WRAWLPSQTI RRACPQCLND PTNQAVLLVW QLPLMLSCPQ HGCWLESYWG MPGRYLQWEI ADAAPRPADD AIACMDRRTW QALTTGFVEL PRRRVHAGLW FRLLRTLLDE LNTPLSHCGS CSASIRHVWE RCGHPLRAGQ SLWRPYEILA PAVQWQMLEA AATAITLIES KALIPRGEQA ALFQTEPYTG FTNGLPAKVP KPEPINHWQR AAQAIDEAII EARHNPETAR LLFTLASYGR RDPESLERLR ATFTKEGIPP EFLSHYEPEW PFAGLRLNDG LSDSF |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniR | TniR | Tn5053.4 | 204 | 4009-4623 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | also calledTniC | ||||||||||
| Protein Sequence: | MLIGYMRVSK ADGSQATDLQ RDALIAAGVD PVHLYEDQAS GMREDRPGLT SCLKALRTGD TLVVWKLDRL GRDLRHLINT VHDLTGRGIG LKVLTGHGAA IDTTTAAGKL VFGIFAALAE FERELIAERT IAGLASARAR GRKGGRPFKM TAAKLRLAMA AMGQPETKVG DLCQELGVTR QTLYRHVSPK GELRPDGEKL LSRI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merE | MerE | Tn5053.4 | 78 | 4676-4912 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | Broad-spectrum mercury transporter | ||||||||||
| Protein Sequence: | MNSPERLPSE THKPITGYLW GALAVLTCPC HLPILAIVLA GTTAGAFIGE YWGIAALTLT GLFVLSVTRL LRAFKDRS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD | MerD | Tn5053.4 | 121 | 4909-5274 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MSAYTVSRLA LDAGVSVHIV RDYLLRGLLR PVACTPGGYG LFDDAALQRL CFVRAAFEAG IGLDALARLC RALDAADGDE AAAQLAVLRQ FVERRREALA DLEVQLATMP TEPAQHAESL P |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA | MerA | Tn5053.4 | 548 | 5291-6937 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercuric ion reductase | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MTEITVNGMT CTSCATHVKD ALEKIPGVNA AVVSYPESRA QVMADTAVSH NQLLAAIAAL GYQGSIRVGD FKDEPKIRDA LEGAGLHIAI IGSGGAAMAA ALKAVEQGAT VTLIERGTIG GTCVNIGCVP SKIMIRAAHI AHLRRESPFD GGIAATVPAI DRSKLLAQQQ ARVDELRHAK YEGILDGNPA ITVLHGEARF KDDQSLVVRL NEGGEREVTF DRCLVATGAS PAVPPIPGLK ESPYWTSTEA LVSDTIPARL AVIGSSVVAL ELAQAFARLG SQVTILARST LFFREDPAIG EAVTAAFRAE GIEVLEHTQA SQVAHVNGEF VLTTGHGELR ADKLLVATGR APNTRSLALD AAGVTVNAQG AIVIDQGMRT SNPNIYAAGD CTDQPQFVYV AAAAGTRAAI NMTGGDAALN LTAMPAVVFT DPQVATVGYS EAEAHHDGIE TDSRTLTLDN VPRALANFDT RGFIKLVIEE GSGRLIGVQA VAPEAGELIQ TAVLAIRNRM TVQELADQLF PYLTMVEGLK LAAQTFNKDV KQLSCCAG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merF | MerF | Tn5053.4 | 81 | 6934-7179 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | mercuric ion transport protein | ||||||||||
| Protein Sequence: | MKDPKTLLRV SIIGTTLVAL CCFTPVLVIL LGVVGLSALT GYLDYVLLPA LAIFIGLTIY AIQRKRQADA CCTPKFNGVK K |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | Tn5053.4 | 91 | 7182-7457 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | periplasmic mercuric ion binding protein | ||||||||||
| Protein Sequence: | MKKLLSALAL AAVVAPVWAA TQTVTLSVPG MTCSACPITV KKAISKVDGV SKVDVTFETR EAVVTFDDAK TSVQKLTKAT EDAGYPSSVK N |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | Tn5053.4 | 116 | 7473-7823 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | mercury ion transport protein | ||||||||||
| Protein Sequence: | MSEPQNGRGA LFTGGLAAIL ASACCLGPLV LIALGFSGAW IGNLTVLEPY RPIFIGVALV ALFFAWRRIY RPSAACKPGE VCAIPQVRAT YKLIFWGVAV LVLVALGFPY VVPFFY |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR | MerR | Tn5053.4 | 144 | 7895-8329 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | regulatory protein (repressor /inducer) | ||||||||||
| Protein Sequence: | MENNLENLTI GVFARTAGVN VETIRFYQRK GLLPEPDKPY GSIRRYGETD VTRVRFVKSA QRLGFSLDEI AELLRLEDGT HCEEASSLAE HKLKDVRERM ADLARMEAVL SDLVCACHAR KGNVSCPLIA SLQGKKEPRS ADAV |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | IS4321 | 334 | 8429-9433 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DEDD | ||||||||||
| Protein Sequence: | MENIALIGID LGKNSFHIHC QDRRGKAVYR KKFTRPKLIE FLATCPATTI AMEACGGSHF MARKLEELGH SPKLISPQFV RPFVKSNKND FVDAEAICEA ASRPSMRFVQ PRTESQQAMR ALHRVRESLV QDKVKTTNQM HAFLLEFGIS VPRGAAVISR LSTILEDNSL PLYLSQLLLK LQQHYHYLVE QIKDLESQLK RKLDEDEVGQ RLLSIPCVGT LTASTISTEI GDGKQYASSR DFAAATGLVP RQYSTGGRTT LLGISKRGNK KIRTLLVQCA RVFIQKLEHQ SGKLADWVRD LLCRKSNFVV TCALANKLAR IAWALTARQQ TYVA |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| IS4321-U67194 | IS4321 | insertion sequence | 8362-9688 | + | 1327 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRL | IS4321 | 8362-8380 | + | 19 | TAATGAGATG GTCACTCCC |
| IRR | IS4321 | 9674-9688 | - | 15 | TCTATGGTCA CTCCC |
1. Ren Y, Ren Y, Zhou Z, Guo X, Li Y, Feng L, Wang L.
Complete genome sequence of Enterobacter cloacae subsp. cloacae type strain ATCC 13047.. J Bacteriol. 2010 May;192(9):2463-4. doi: 10.1128/JB.00067-10. Epub 2010 Mar 5. PubMed ID:
20207761
.