Mobile element type:
Transposon
Name:
Tn5053.2
Synonyms:
Accession:
Tn5053.2-CP024682
Family:
Tn402
Group:
Tn5053
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Citrobacter freundii UMH14
Date of Isolation:
2017
Country:
U.s.a.
Molecular Source:
plasmid pUMH14_2
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRt | 1-25 | + | 25 | TGTCGTTTTC AGAAGACGAC CGCAC |
| repeat t1 | 9-27 | + | 19 | TCAGAAGACG ACCGCACCA |
| repeat t2 | 49-67 | - | 19 | TCAGGAGCCG TATGCACAC |
| repeat t3 | 78-96 | + | 19 | TCAGGAGTCG TCTGCACCA |
| repeat t4 | 110-128 | + | 19 | TCAATACTCG TGTGCACCA |
| repeat i3 | 8579-8597 | - | 19 | TCAGGCAACG ACGGGCTGC |
| repeat i2 | 8621-8639 | - | 19 | TCAGAAGCCG ACTGCACTA |
| IRi | 8638-8670 | - | 33 | TGTCGTTTTC AGAAGACGGC TGCACTGAAC GTC |
| repeat i1 | 8644-8662 | - | 19 | TCAGAAGACG GCTGCACTG |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| r6 | Tn5053.2 | 3826-3840 | - | 15 | GCGCAAACGT TCCAG |
| r5 | Tn5053.2 | 3872-3885 | + | 14 | AGTTTTTGTC ACAT |
| r4 | Tn5053.2 | 3877-3890 | - | 14 | TCGTAATGTG ACAA |
| r3 | Tn5053.2 | 3928-3941 | + | 14 | GGGTTAAGTG ACAG |
| res | Tn5053.2 | 3969-4003 | + | 35 | ACACTGTCAC ATAATCGAAC GTATATGTGA CAGGT |
| r2 | Tn5053.2 | 3972-3985 | - | 14 | CGATTATGTG ACAG |
| r1 | Tn5053.2 | 3988-4001 | + | 14 | CGTATATGTG ACAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tniA | Tn5053.2 | 144-1823 | + | Transposase | |
| tniB | Tn5053.2 | 1826-2734 | + | Accessory Gene | |
| tniQ | Tn5053.2 | 2731-3948 | + | Accessory Gene | Target Site Selection |
| tniR | Tn5053.2 | 4009-4623 | + | Accessory Gene | Resolvase |
| merE | Tn5053.2 | 4676-4912 | - | Passenger Gene | Heavy Metal Resistance |
| merD | Tn5053.2 | 4909-5274 | - | Passenger Gene | Heavy Metal Resistance |
| merA_p | Tn5053.2 | 5291-6667 | - | Passenger Gene | Heavy Metal Resistance |
| ahpD | Tn5053.2 | 6824-7399 | - | Passenger Gene | Other |
| araC family | Tn5053.2 | 7463-8317 | + | Passenger Gene | Other |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniA | TniA | Tn5053.2 | 559 | 144-1823 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MTSDTPPIAA QGVATLPDEA WAQARHRTEI IGPLAALEVV GHEAADEAAQ ALGLSRRQVY VLIRRARQGT GLVTDLTPGR SGGGKGKGRL PEPVERIIRE LLQKRFLTKQ KRSLAAFHRE VAQACKTQKL PVPARNTVAQ RIAGLHPAKI ARSRGGQDAA RPLQGAGGIP PEVTMPLEQV QIDHTVIDLI VVDERDRQPI GRPYLTLAID VFTRCVLGMV VTLEAPSAVS VGLCLAHAAC DKRPWLEGLN VEMDWPMSGK PRLLYLDNAA EFKSEALRRG CEQHGIRLDY RPPGQPHYGG IVERIIGTAM QMIHDELPGT TFSNPGQRGE YDSEKMATLT LRELERWLAL AVGTYHGSVH NGLLQPPAAR WAEAVERVGV PAVVTRPTAF LVDFLPVIRR TLTRTGFVID HIHYYADALK PWIARRERLP AFLIRRDPRD ISRIWVLEPE GQHYLEIHYR TLSHPAVTLW EQRQALAKLR QLGREQVDES ALFRMIGQMR EIVTTAQKAT RKARRDADRR QHLKTSEPPA KPIPPDVDMA DPQADNLPPA KPFDQIEEW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniB | TniB | Tn5053.2 | 302 | 1826-2734 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Function: | is needed for transposition of Tn5053 | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | homologous to TnsC protein of Tn7 putative ATP-binding protein | ||||||||||
| Protein Sequence: | MDEYPVIDLS HLLPAAQGLA RLPADERIQR IRADRWIGYP RAVEALNRLE TLYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRAHPVGTD ADQEHIPVLV VQMPSEPSVI RFYVALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRE AYLAIRSDDQ LENRFEPMLL PPWEANEDCC SLLASFAASL PLRRPSPIAT LDMARYLLTR SEGTIGELAH LLVAAAVAAV ESGEEAINHR TLSMADYIGP SERRRQFERE LM |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniQ | TniQ | Tn5053.2 | 405 | 2731-3948 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Target Site Selection | ||||||||||
| Function: | is needed for transposition of Tn5053 | ||||||||||
| Protein Sequence: | MKSAPRWPLH PAPKEGEALS SWLNRVAACY QMDVHELLAH DLGHSQLDDL DTAPSLSLLT ALCQRSGVEL ERLRSMSLAG WVPWLLDSLD DSVPAALETY TFQCAVLLPK RTRKVRSITR WRAWLPSQTI RRACPQCLND PTNQAVLLVW QLPLMLSCPQ HGCWLESYWG MPGRYLQWEI ADAAPRPADD AIACMDRRTW QALTTGFVEL PRRRVHAGLW FRLLRTLLDE LNTPLSHCGS CSASIRHVWE RCGHPLRAGQ SLWRPYEILA PAVQWQMLEA AATAITLIES KALIPRGEQA ALFQTEPHTG FTNGLPAKVP KPEPINHWQR AAQAIDEAII EARHNPETAR LLFTLASYGR RDPESLERLR ATFTKEGIPP EFLSHYEPEW PFAGLRLNDG LSDSF |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniR | TniR | Tn5053.2 | 204 | 4009-4623 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | also calledTniC | ||||||||||
| Protein Sequence: | MLIGYMRVSK ADGSQATDLQ RDALIAAGVD PVHLYEDQAS GMREDRPGLT SCLKALRTGD TLVVWKLDRL GRDLRHLINT VHDLTGRGIG LKVLTGHGAA IDTTTAAGKL VFGIFAALAE FERELIAERT IAGLASARAR GRKGGRPFKM TAAKLRLAMA AMGQPETKVG DLCQELGVTR QTLYRHVSPK GELRPDGEKL LSRI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merE | MerE | Tn5053.2 | 78 | 4676-4912 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | Broad-spectrum mercury transporter | ||||||||||
| Protein Sequence: | MNSPERLPSE THKPITGYLW GALAVLTCPC HLPILAIVLA GTTAGAFIGE YWGIAALTLT GLFVLSVTRL LRAFKDRS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD | MerD | Tn5053.2 | 121 | 4909-5274 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MSAYTVSRLA LDAGVSVHIV RDYLLRGLLR PVACTPGGYG LFDDAALQRL CFVRAAFEAG IGLDALARLC RALDAADGDE AAAQLAVLRQ FVERRREALA DLEVQLATMP TEPAQHAESL P |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA_p | MerA_p | Tn5053.2 | 458 | 5291-6667 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | N-ter deletion due to insertion of ahpD | ||||||||||
| Protein Sequence: | IGSGGAAMAA ALKAVEQGAT VTLIERGTIG GTCVNIGCVP SKIMIRAAHI AHLRRESPFD GGIAATVPAI DRSKLLAQQQ ARVDELRHAK YEGILDGNPA ITVLHGEARF KDDQSLVVRL NEGGEREVTF DRCLVATGAS PAVPPIPGLK ESPYWTSTEA LVSDTIPARL AVIGSSVVAL ELAQAFARLG SQVTILARST LFFREDPAIG EAVTAAFRAE GIEVLEHTQA SQVAHVNGEF VLTTGHGELR ADKLLVATGR APNTRSLALD AAGVTVNAQG AIVIDQGMRT SNPNIYAAGD CTDQPQFVYV AAAAGTRAAI NMTGGDAALN LTAMPAVVFT DPQVATVGYS EAEAHHDGIE TDSRTLTLDN VPRALANFDT RGFIKLVIEE GSGRLIGVQA VAPEAGELIQ TAVLAIRNRM TVQELADQLF PYLTMVEGLK LAAQTFNKDV KQLSCCAG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | ahpD | AhpD | Tn5053.2 | 191 | 6824-7399 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | putative alkylhydroperoxidase || proteinID: ACA14447.1 | ||||||||||
| Protein Sequence: | MPTCSCTSPT TEIDMTQIAP LTIDTADAAT AATLKAVKAK LGMVPNLFAT LAHAPAALNG YLGLSETLGT GRLNASQREI VALAAGQANR CQYCLSAHTL IGKGAGLSAE AIAAARTGQA ANALDDAIAG FARALVEQRG VVSADAMANY RRAGLDDGLI LEVIANVALN TLTNYTNHIA DPTVDFPVVA V |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | araC family | AraC family | Tn5053.2 | 284 | 7463-8317 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Protein Sequence: | MESGMADRLA GLLRHYSLSA RVFHSGAFCG QHHYAAPHGY IHLVRRGPIT ARSPVHEDLL VTEPSLLFYP RVASHRFVAA PGDTAEQLCA EVDLGASTGN PLAMALPSML LIPLADLPGL GPTLELLFAE AERDQCGRQA AIDRLCELLL IQLLRYLMDG RLGATGLLAG LADPKLARAI TAMHDAPQTA WSLEALAAKA GMSRARFAAA FKDAVGVTPG DYLADWRMNV SCTLLKQGRP VAVVADRVGY GSPNALARAF RVRMGCAPRD WLAQQRGDAA LSGS |
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1. Anderson MT, Mitchell LA, Zhao L, Mobley HLT.
Citrobacter freundii fitness during bloodstream infection.. Sci Rep. 2018 Aug 7;8(1):11792. doi: 10.1038/s41598-018-30196-0. PubMed ID:
30087402
.