Mobile element type:
Transposon
Name:
Tn5053
Synonyms:
Accession:
Tn5053-L40585.1
Family:
Tn402
Group:
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Xanthomonas sp. W17
Date of Isolation:
1993
Country:
Former urss
Molecular Source:
chromosome
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRt | 1-25 | + | 25 | TGTCGTTTTC AGAAGACGAC CGCAC |
| repeat t1 | 9-27 | + | 19 | TCAGAAGACG ACCGCACCA |
| repeat t2 | 49-67 | - | 19 | TCAGGAGCCG TATGCACAC |
| repeat t3 | 78-96 | + | 19 | TCAGGAGTCG TCTGCACCA |
| repeat t4 | 110-128 | + | 19 | TCAATACTCG TGTGCACCA |
| IR Tn21-like | 8346-8383 | - | 38 | GGAGTCGCCT CAGAAAACGG AAAATAAAGC ACGCTAAG |
| repeat i4 | 8356-8374 | - | 19 | TCAGAAAACG GAAAATAAA |
| repeat i2 | 8398-8416 | + | 19 | CCGTGCGGTC GACTTTTGA |
| IRi | 8420-8447 | - | 28 | TGTCACTTTC AGAAGGCGAC AGCACGAA |
| repeat i1 | 8421-8439 | - | 19 | TCAGAAGGCG ACAGCACGA |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| r6 | Tn5053 | 3826-3840 | - | 15 | GCGCAAACGT TCCAG |
| r5 | Tn5053 | 3872-3885 | + | 14 | AGTTTTTGTC ACAT |
| r4 | Tn5053 | 3877-3890 | - | 14 | TCGTAATGTG ACAA |
| r3 | Tn5053 | 3928-3941 | + | 14 | GGGTTAAGTG ACAG |
| res | Tn5053 | 3969-4003 | + | 35 | ACACTGTCAC ATAATCGAAC GTATATGTGA CAGGT |
| r2 | Tn5053 | 3972-3985 | - | 14 | CGATTATGTG ACAG |
| r1 | Tn5053 | 3988-4001 | + | 14 | CGTATATGTG ACAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tniA | Tn5053 | 144-1823 | + | Transposase | |
| tniB | Tn5053 | 1826-2734 | + | Accessory Gene | |
| tniQ | Tn5053 | 2731-3948 | + | Accessory Gene | Target Site Selection |
| tniR | Tn5053 | 4009-4623 | + | Accessory Gene | Resolvase |
| merE | Tn5053 | 4676-4912 | - | Passenger Gene | Heavy Metal Resistance |
| merD | Tn5053 | 4909-5274 | - | Passenger Gene | Heavy Metal Resistance |
| merA | Tn5053 | 5291-6937 | - | Passenger Gene | Heavy Metal Resistance |
| merF | Tn5053 | 6934-7179 | - | Passenger Gene | Heavy Metal Resistance |
| merP | Tn5053 | 7182-7457 | - | Passenger Gene | Heavy Metal Resistance |
| merT | Tn5053 | 7473-7823 | - | Passenger Gene | Heavy Metal Resistance |
| merR | Tn5053 | 7895-8329 | + | Passenger Gene | Heavy Metal Resistance |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniA | TniA | Tn5053 | 559 | 144-1823 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | homologous to TnsB of Tn7 | ||||||||||
| Protein Sequence: | MTSDTPPIAA QGVATLPDEA WAQARHRTEI IGPLAALEVV GHEAADEAAQ ALGLSRRQVY VLIRRARQGT GLVTDLTPGR SGGGKGKGRL PEPVERIIRE LLQKRFLTKQ KRSLAAFHRE VAQACKTQKL PVPARNTVAQ RIAGLHPAKI ARSRGGQDAA RPLQGAGGIP PEVTMPLEQV QIDHTVIDLI VVDERDRQPI GRPYLTLAID VFTRCVLGMV VTLEAPSAVS VGLCLAHAAC DKRPWLEGLS VEMDWPMSGK PRLLYLDNAA EFKSEALRRG CEQHGIRLDY RPPGQPHYGG IVERIIGTAM QMIHDELPGT TFSNPGQRGE YDSEKMATLT LRELERWLAL AVGTYHGSVH NGLLQPPAAR WAEAVERVGV PAVVTRPTAF LVDFLPVIRR TLTRTGFVID HIHYYADALK PWIARRERLP AFLIRRDPRD ISRIWVLEPE GQHYLEIHYR TLSHPAVTLW EQRQALAKLR QLGREQVDES ALFRMIGQMR EIVTTAQKAT RKARRDADRR QHLKTSEPPA KPIPPNVDMA DPQADNLPPA KPFDQIEEW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniB | TniB | Tn5053 | 302 | 1826-2734 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Function: | is needed for transposition of Tn5053 | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | homologous to TnsC protein of Tn7 putative ATP-binding protein | ||||||||||
| Protein Sequence: | MDEYPVIDLS HLLPAAQGLA RLPADERIQR IRADRWIGYP RAVEALNRLE TLYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRAHPVGTD ADQEHIPVLV VQMPSEPSVI RFYVALLAAM GAPLRPRPRL TEMEQLALAL LRMVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRE AYLAIRSDDQ LENRFEPMLL PPWEANEDCC SLLASFAASL PLRRPSPIAT LDMARYLLTR SEGTIGELAH LLVAAAVAAV ESGEEAINHR TLSMADYIGP SERRRQFERE LM |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniQ | TniQ | Tn5053 | 405 | 2731-3948 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Target Site Selection | ||||||||||
| Function: | is needed for transposition of Tn5053 | ||||||||||
| Protein Sequence: | MKSAPRWPLH PAPKEGEALS SWLNRVAACY QMDVHELLAH DLGHSQLDDL DTAPSLSLLT ALCQRSGVEL ERLRSMSLAG WVPWLLDSLD DSVPAALETY TFQCAVLLPK RTRKVRSITR WRAWLPSQTI RRACPQCLND PTNQAVLLVW QLPLMLSCPQ HGCWLESYWG MPGRYLQWEI ADAAPRPADD AIACMDRRTW QALTTGFVEL PRRRVHAGLW FRLLRTLLDE LNTPLSHCGS CSASIRHVWE RCGHPLRAGQ SLWRPYEILA PAVQWQMLEA AATAITLIES KALIPRGEQA ALFQTEPHTG FTNGLPAKVP KPEPINHWQR AAQAIDEAII EARHNPETAR LLFTLASYGR RDPESLERLR ATFTKEGIPP EFLSHYEPEW PFAGLRLNDG LSDSF |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniR | TniR | Tn5053 | 204 | 4009-4623 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | also called TniC | ||||||||||
| Protein Sequence: | MLIGYMRVSK ADGSQATDLQ RDALIAAGVD PVHLYEDQAS GMREDRPGLT SCLKALRTGD TLVVWKLDRL GRDQRHLINT VHDLTGRGIG LKVLTGHGAA IDTTTAAGKL VFGIFAALAE FERELIAERT IAGLASARAR GRKGGRPFKM TAAKLRLAMA AMGQPETKVG DLCQELGVTR QTLYRHVSPK GELRPDGEKL LSRI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merE | MerE | Tn5053 | 78 | 4676-4912 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | Broad-spectrum mercury transporter | ||||||||||
| Protein Sequence: | MNSPERLPSE THKPITGYLW GALAVLTCPC HLPILAIVLA GTTAGAFIGE YWGIAALTLT GLFVLSVTRL LRAFKDRS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD | MerD | Tn5053 | 121 | 4909-5274 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MSAYTVSRLA LDAGVSVHIV RDYLLRGLLR PVACTPGGYG LFDDAALQRL CFVRAAFEAG IGLDALARLC RALDAADGDE AAAQLAVLRQ FVERRREALA DLEVQLATMP TEPAQHAESL P |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA | MerA | Tn5053 | 548 | 5291-6937 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercuric ion reductase | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MTEITVNGMT CTSCATHVKD ALEKIPGVNA AVVSYPESRA QVMADTAVSH NQLLAAIAAL GYQGSIRVGD FKDEPKIRDA LEGAGLHIAI IGSGGAAMAA ALKAVEQGAT VTLIERGTIG GTCVNIGCVP SKIMIRAAHI AHLRRESPFD GGIAATVPAI DRSKLLAQQQ ARVDELRHAK YEGILDGNPA ITVLHGEARF KDDQSLVVRL NEGGEREVTF DRCLVATGAS PVVPPIPGLK ESPYWTSTEA LVSDTIPARL AVIGSSVVAL ELAQAFARLG SQVTILARST LFFREDPAIG EAVTAAFRAE GIEVLEHTQA SQVAHVNGEF VLTTGHGELR ADKLLVATGR APNTRSLALD AAGVTVNAQG AIVIDQGMRT SNPNIYAAGD CTDQPQFVYV AAAAGTRAAI NMTGGDAALN LTAMPAVVFT DPQVATVGYS EAEAHHDGIE TDSRTLTLDN VPRALANFDT RGFIKLVIEE GSGRLIGVQA VAPEAGELIQ TAVLAIRNRM TVQELADQLF PYLTMVEGLK LAAQTFNKDV KQLSCCAG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merF | MerF | Tn5053 | 81 | 6934-7179 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | mercuric ion transport protein | ||||||||||
| Protein Sequence: | MKDPKTLLRV SIIGTTLVAL CCFTPVLVIL LGVVGLSALT GYLDYVLLPA LAIFIGLTIY AIQRKRQADA CCTPKFNGVK K |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | Tn5053 | 91 | 7182-7457 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | periplasmic mercuric ion binding protein | ||||||||||
| Protein Sequence: | MKKLLSALAL AAVVAPVWAA TQTVTLSVPG MTCSACPITV KKAISKVDGV SKVDVTFETR EAVVTFDDAK TSVQKLTKAT EDAGYPSSVK N |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | Tn5053 | 116 | 7473-7823 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | mercury ion transport protein | ||||||||||
| Protein Sequence: | MSEPQNGRGA LFTGGLAAIL ASACCLGPLV LIALGFSGAW IGNLTVLEPY RPIFIGVALV ALFFAWRRIY RPSAACKPGE VCAIPQVRAT YKLIFWGVAV LVLVALGFPY VVPFFY |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR | MerR | Tn5053 | 144 | 7895-8329 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | regulatory protein (repressor /inducer) | ||||||||||
| Protein Sequence: | MENNLENLTI GVFARTAGVN VETIRFYQRK GLLPEPDKPY GSIRRYGETD VTRVRFVKSA QRLGFSLDEI AELLRLEDGT HCEEASSLAE HKLKDVRERM ADLARMEAVL SDLVCACHAR KGNVSCPLIA SLQGKKEPRS ADAV |
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1. Kholodii GY, Yurieva OV, Lomovskaya OL, Gorlenko Z, Mindlin SZ, Nikiforov VG.
Tn5053, a mercury resistance transposon with integron's ends.. J Mol Biol. 1993 Apr 20;230(4):1103-7. doi: 10.1006/jmbi.1993.1228. PubMed ID:
8387603
.
2. Kholodii GY, Mindlin SZ, Bass IA, Yurieva OV, Minakhina SV, Nikiforov VG. Four genes, two ends, and a res region are involved in transposition of Tn5053: a paradigm for a novel family of transposons carrying either a mer operon or an integron.. Mol Microbiol. 1995 Sep;17(6):1189-200. doi: 10.1111/j.1365-2958.1995.mmi_17061189.x. PubMed ID: 8594337 .
2. Kholodii GY, Mindlin SZ, Bass IA, Yurieva OV, Minakhina SV, Nikiforov VG. Four genes, two ends, and a res region are involved in transposition of Tn5053: a paradigm for a novel family of transposons carrying either a mer operon or an integron.. Mol Microbiol. 1995 Sep;17(6):1189-200. doi: 10.1111/j.1365-2958.1995.mmi_17061189.x. PubMed ID: 8594337 .