Mobile element type:
Transposon
Name:
Tn5046
Synonyms:
Accession:
Tn5046-Y18360.1
Family:
Tn3
Group:
Tn3
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Pseudomonas sp. LS46-6
Date of Isolation:
2001
Country:
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-46 | + | 46 | GGGGTTTGGG GAGCAATGGA ACAGAAAGTG CACTTAAGCC CTACCA |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | Tn5046 | 972-1107 | + | 136 | AGTACCGTTT ACTCGACTAC GTTAGTAATA GTTGAACTTT GATTAAGCGT ACCAGTTATT TGAACCGTAG CGCAGGTAGG TTAACCAGCC GATTCCTGTC CACTTAAAGT TCGGCAAAAC GAAGGTTTTT TTGAAC |
| res_site_III | Tn5046 | 982-1023 | + | 42 | ACTCGACTAC GTTAGTAATA GTTGAACTTT GATTAAGCGT AC |
| res_site_II | Tn5046 | 1026-1060 | + | 35 | GTTATTTGAA CCGTAGCGCA GGTAGGTTAA CCAGC |
| res_site_I | Tn5046 | 1080-1107 | + | 28 | GTTCGGCAAA ACGAAGGTTT TTTTGAAC |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpR | Tn5046 | 41-967 | - | Accessory Gene | Resolvase |
| RHH_6 | Tn5046 | 1169-1408 | + | Passenger Gene | Antitoxin |
| PIN_3 | Tn5046 | 1408-1818 | + | Passenger Gene | Toxin |
| tnpA | Tn5046 | 1822-4818 | + | Transposase | |
| urf-2-CAC80083.1 | Tn5046 | 4956-5294 | - | Passenger Gene | Hypothetical |
| urf-1-CAC80082.1 | Tn5046 | 5291-5527 | - | Passenger Gene | Hypothetical |
| orfY-WP_004574523.1 | Tn5046 | 5882-6298 | - | Passenger Gene | Hypothetical |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn5046 | 308 | 41-967 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MKIGYARVST RDQNADLQVD ALKQAGCERI YQDIASGAKS ARPELDKLLA HVRAGDTVVI WKLDRLGRSL KHLVELVGEL AERKVGLQSL NDPIDTTHAQ GRLVFNLFAL LAEFERELIR ERTQAGLSAA RARGRIGGRP KGFPAKAEAT SMAAETLYRE GRLSVSAIGE KLHISKSTLY SYLRHRGVEI GAHQKSAQPR GQQRNVASPA EPAAEQVATV TLRLAVVNNS KFVRGRKRAK ENFERYCLEP YSMKRLESGN YELAIPYRSD DELDKTVHDL LTEISQEADM RNCFIEADAW EEGSERRW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | RHH_6 | RHH_6 | Tn5046 | 79 | 1169-1408 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antitoxin | ||||||||||
| Function: | transposition protein, putative | ||||||||||
| Sequence Family: | RHH_6 (Pfam:PF16762) | ||||||||||
| Comment: | VapB | ||||||||||
| Protein Sequence: | MNTIRWNVAV SADTDQSLRM FLASQGGGRK GDLSRFIEEA VRAHILELTA EQAKAANAHL SEAELTEAVD EALDWARKR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | PIN_3 | PIN_3 | Tn5046 | 136 | 1408-1818 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Toxin | ||||||||||
| Function: | transposition protein, putative | ||||||||||
| Sequence Family: | PIN_3 (Pfam:PF13470) | ||||||||||
| Target: | single stranded RNA | ||||||||||
| Protein Sequence: | MRVVLDTNIL FSALISPHGA PDAIYRAWRA ARFEVVTSRM QLDETRRASR YPKLQAILQP AKVGAMINNL QRAVVLERLT IEVEADDPDD SFLLAMALAG DADYLVTGDR RAGLLQRGHI ERTRIVTPAV FCAEVL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn5046 | 998 | 1822-4818 | + |
|---|
| Class: | Transposase | ||||||||||
| Function: | transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPVGFLTQEQ RDGFGRYVDA PSREELERYF HLSDDDHKIL LPLRGEHNRL GYATQLTSIR YLGTFPDDFS AVPQEVLQAL SRQLGITDPT CIQAYAETRQ RQRHAAEIQE RYGYRVFADS SVGFRLARWL YALCWTGTDR PGELFNRATT WLLTHKVLLP GVTVLERFIA QLRSRVEERL WLTLGRSVSE AQRQQLQDLL LVAEGNRSSR LDQLRSGPVM VSGPALIRAL RRLDDVRGIG IALPAAAHIP PSRIAALARF ANTAKVTAIN RLPASRRMAT LVAFALCLEA TAHDDALEVL EALLRDLFSN AEKADKKARM RSLKDLDRSA ATLAAACKVV LDSSISDDKV RARLFNDLPR ASLEKALEDV NALIRPVDDV YFLALEARYR SVRRFLPDLL KHIRFGFSPA GKGVVAGLDW LQLNLPRRKP EDDAPQEIVA KAWQKHITRE DGSLDMGAYV FCTLDALRTA LRRRDVFVSP SWRYADPRLG LLDGAEWLAA RPIICRSLGL TIDAGTTLDA LSAELDATWQ AVAARLPDNP AIQLSENREG KTELSLGTLS KLEEPNSLLQ LRAAVADLMP RVDLPEILLE IAARTGFAEA FTHVSERNAR ADNLVTSLCA VLLGGACNTG LEPLIRTDNQ ALRRDRLSWV SQNYIRDDTL SVANAILVGA QSQLELAQVC GGGEVPPADG MRFVVPVRTV HAGPNPKYFG TGRGVTWYNL ISDQFSGLNA ITVPGTLRDS LVLLAVVLEH ETELQPTQIM TDTGVYSNVV FGLFRLLGYH FSPRLADVGG TRFWRTRPEA DYGKLNGLAR QSVKLDLIAE HWDDLLRLAG SLKLGRVPAT GIMRTLQTGD RPTRLAQALA EFGRIEKTLH TLTYIDDESK RRATLTQLNR GEGRHSLARP VFHGKRGELR QRYREGQEDQ LGPLGLVVNI IVLWNTLYMT AAVERLRQHG YPVLEEDVAR LSPLIHEHIN MLGRYSFAVP EEVTRGELRP VRNPDDDQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | urf-2-CAC80083.1 | Urf-2-CAC80083.1 | Tn5046 | 112 | 4956-5294 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Protein Sequence: | MTRSEHGATT TAELGWEAAG RSALSHGGKA LLPFLGHGGT PIMNTNNTST ISCCECCKDI PLDAALTPEG SEYVVHFCGL ECYQRFMSRA DKRAASEPDH PCTQKVSTRQ DG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | urf-1-CAC80082.1 | Urf-1-CAC80082.1 | Tn5046 | 78 | 5291-5527 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Protein Sequence: | MRNAVHVESS KVFRWRAYTW GVLAALTCPC HLPVLALLLS GTAAGAFVSE QWSLATLVLA VFFILFLRAA LQAFRKRP |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | orfY-WP_004574523.1 | OrfY-WP_004574523.1 | Tn5046 | 138 | 5882-6298 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Protein Sequence: | MNDTASDLGS RDSLRKTWQV ISLWGLPAVV ATIALVLADR RPALFLAASA ALLVMGGACL VNAARCRRLH CYITGPYFLM LALGALLAYG FAPNGEYHSR LWLLLALGVT PLLIWLPERL AGRKYLSALV CGEDRECR |
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1. Mindlin S, Kholodii G, Gorlenko Z, Minakhina S, Minakhin L, Kalyaeva E, Kopteva A, Petrova M, Yurieva O, Nikiforov V.
Mercury resistance transposons of gram-negative environmental bacteria and their classification.. Res Microbiol. 2001 Nov;152(9):811-22. doi: 10.1016/s0923-2508(01)01265-7. PubMed ID:
11763242
.