Mobile element type:
Transposon
Name:
Tn5045.1
Synonyms:
Tn1013
Accession:
Tn5045.1-NC_008357.1
Family:
Tn3
Group:
Tn21
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Pseudomonas aeruginosa
Date of Isolation:
2007
Country:
Molecular Source:
plasmidpBS228
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-37 | + | 37 | GGGGAGCCCG CAGAATTCGG AAAAAATCGT ACGCTAA |
| IRR | 7760-7796 | - | 37 | GGGGAGCCCG CAGAATTCGG AAAAAATCGT ACGCTAA |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res_site_I | Tn5045.1 | 4048-4077 | + | 30 | TGTCAGCCTG AGCTATACCC TAACTGGATG |
| res_site_II | Tn5045.1 | 4107-4133 | + | 27 | TAGAATCCGC TTTCACATTC TTTGACA |
| res_site_III | Tn5045.1 | 4136-4167 | + | 32 | TGCTTGCCAA GGTCATAGAT TTCAGCCTGA CA |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| taoD | Tn5045.1 | 83-1045 | - | Passenger Gene | Other |
| taoC' | Tn5045.1 | 1167-1370 | - | Passenger Gene | Other |
| taoB | Tn5045.1 | 1370-2221 | - | Passenger Gene | Other |
| taoA | Tn5045.1 | 2236-3723 | - | Passenger Gene | Other |
| tnpR | Tn5045.1 | 4233-4793 | + | Accessory Gene | Resolvase |
| tnpA | Tn5045.1 | 4797-7763 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | taoD | TaoD | Tn5045.1 | 320 | 83-1045 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Sequence Family: | SdiA-Regulated Motif Containing Protein (Pfam: PF06977) | ||||||||||
| Comment: | DNA binding protein | ||||||||||
| Protein Sequence: | MYLMAKNWLS RTRKASAWMW ALLCLVLLTV FQVRTHHLDD RLYFWIKTSW HTDDWQERSV WLPGYRVELD AKAVPGVDNN LSGLTFDPDL NLLWAVTNGP NELLALSRDG DVERRYNLDG FHDVEAVSYA GNGQLVIAEE RRQSLVIVDV PIAEDGKLSP DRSLSRDQYP ALTLALGKED NKGLEGLAYD LKGDRLFVTK ERDPRQLLEV GGLRASLAGG FSLHVRDLSN LVKDKVFATD LSSVVFDQQS GHLILLSDES KLLIEMTDEG KVVSFRSLAR GFAGLLKGIP QAEGVTIDDE GYLYVVSEPN LFYRFTREPD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | taoC' | TaoC' | Tn5045.1 | 67 | 1167-1370 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | truncated compared to Tn1404 due to frameshift-causing deletion | ||||||||||
| Protein Sequence: | MSMDDALDLA HFKTLLEQRA AELDQLLEDA ESRSQSVELD QSKVGRLSRI DALQQQAMMA GVTNAAS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | taoB | TaoB | Tn5045.1 | 283 | 1370-2221 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Sequence Family: | Universal Stess Protein (Pfam:PF00582) | ||||||||||
| Comment: | also known as uspA1 | ||||||||||
| Protein Sequence: | MTQVIACIDA SASAPAVCDY AAWASLSLEA PLTFLHVLDQ RQYPVTADLS GNIGLGSREH LLDELASLDE QRGKLALEQG RIMLAAAKER AIKDGVAAPE SKQRHGDLLE SLQELQTETR LLVIGRQGES SGGLSQHVGS QLESVIRIMH RPILVTPANF QKPESAMLAF DGGATTRKGV EMLAASPLLK GLPIHLVMVG PVSDESSAQL DWAQKVLLNA GFTVRAEALN GEIEPTLHAY QKEHGIDLLV MGAYGHSRIR QFLVGSTTTS MLRTTTSPLL LLR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | taoA | TaoA | Tn5045.1 | 495 | 2236-3723 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Sequence Family: | SulP Family Inorganic Anion Transporter (Pfam:PF00916) | ||||||||||
| Comment: | also known as sulP sulphate permease | ||||||||||
| Protein Sequence: | MLHSLKQTWL SNIRGDILAG IVVALALIPE AIAFSIIAGV DPKVGLYASF CIAVVIAFVG GRPGMISAAT GAMALLMVTL VKNHGLEYLL AATLLCGVLQ IAAGYLKLGS LMRFVSRSVV TGFVNALAIL IFMAQLPELT NVTWHVYAMT AAGLGIIYLF PYVPKIGKLI PSPLVCIIVL TAVAMSVGLD IRTVGDMGEL PDTLPIFLWP DVPLTFETLA IIFPYSAALA VVGLLESMMT ATIVDDLTDT SSDKNRECKG QGVANIASGL IGGMAGCAMI GQSIINVKSG GRSRLSSLAA GVFLLLMVVF LGDWLKQIPM AALVAVMIMV SIGTFSWDSL RNLKKHPLST NIVMVVTVVV VVATHNLAFG VLAGVLLAAM FFANKVGHYM AISSLLDEAG EHRSYNVTGQ VFFSSADKFV AAFDFKEALN KVTIDLNRAH FWDITAVAAL DKVVIKFRRE GTEVEVLGLN EASATIVDRF GVHDKPDAID QLMGH |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn5045.1 | 186 | 4233-4793 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MQGQRIGYVR VSSYDQNPER QLEQVEVGKL FTDKASGRDT QRPQLEAMLG FVREGDTVVV HSMDRLARNL DDLRRLVQKL TQRGVRIEFL KEGLVFTGDD SPMANLMLSV MGAFAEFERA LIRERQREGI ALAKQRGAYR GRKKALSDEQ AATLRQRASA GEPKAQLARE FNISRETLYQ YLRTDD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn5045.1 | 988 | 4797-7763 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | putative transposase of Tn5045 identical to tnpR of Tn1013 in pBS228 | ||||||||||
| Protein Sequence: | MPRRLILSAT ERDTLLALPE SQDDLIRYYT FNDSDLSLIR QRRGDANRLG FAVQLSLLRY PGYALGSDSE LPEPVIQWVA KQVQADPTSW AKYGERDVTR REHAQELRNY LQLAPFGLSD FRALVRELTE LAQQTDKGLL LAGQALESLR QKRRILPALS VIDRACSEAI ARANRRVYRA LVEPLTDSHR AKLDELLKLK AGSSITWLTW LRQAPLKPNS RHMLEHIERL KTFQLVDLPE ALGRHIHQNR LLKLAREGGQ MTPKDLCKFE PQRRYATLAA VVLESTATVI DELVDLHDRI LVKLFSGAKH KHQQQFQKQG KAINDKVRLY SKIGQALLEA KESGSDPYAA IEAVIPWDEF TESVSEAELL ARPEGFDHLH LVGENFATLR RYTPALLEVL ELRAAPAAQG VLAAVQTLRE MNADNLRKVP ADAPTAFIKQ RWRPLVITPE GLDRRFYEIC ALSELKNALR SGDIWVKGSR QFRDFDDYLL PAERFAALKH AQALPLAINP NRNQYLEERL QLLDEQLATV TRLAKDNELP DAILTESGLK ITPLDSAVPN TAQALIDQTS QLLPRIKITE LLMDVDDWTG FSRHFTHLKD GAEAKDRTLL LAAILGDAIN LGLTKMAESS PGLTYAKLSW LQAWHIRDET YSAALAELVN HQYRHTFAAH WGDGTTSSSD GQRFRAGGRG ESTGHVNPKY GSEPGRLFYT HISDQYAPFS TRVVNVGVRD STYVLDGLLY HESDLRIEEH YTDTAGFTDH VFALMHLLGF RFAPRIRDLG ETKLYVPNSV QDYPTLRPMV GGTLNIKHVR AHWDDILRLA SSIKQGTVTA SLMLRKLGSY PRQNGLAVAL RELGRIERTL FILDWLQSVE LRRRVHAGLN KGEARNSLAR AVFFNRLGEI RDRSFEQQRY RASGLNLVTA AIVLWNTVYL ERATQAMGEA GKSVDGELLQ YLSPLGWEHI NLTGDYVWRQ SRRLEDGKFR PLRLPGKP |
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1. Haines AS, Jones K, Batt SM, Kosheleva IA, Thomas CM.
Sequence of plasmid pBS228 and reconstruction of the IncP-1alpha phylogeny.. Plasmid. 2007 Jul;58(1):76-83. doi: 10.1016/j.plasmid.2007.01.001. Epub 2007 Feb 23. PubMed ID:
17320955
.