Mobile element type:
Transposon
Name:
Tn5044
Synonyms:
Accession:
Tn5044-Y17691.1
Family:
Tn3
Group:
Tn3
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Xanthomonas campestris TAP44-3
Date of Isolation:
2000
Country:
Russia
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IR | 1-39 | + | 39 | GGGGTTTGGG GAGCAATGGA ACCAAAAACC AACGTAAGC |
| IR | 10802-10840 | - | 39 | GGGGTTTGGG GAGCAATGGA ACCAAAAACC AACGTAAGC |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | Tn5044 | 972-1107 | + | 136 | AGTACCGTTT ACTCGACTAC GTTAGTAATA GTTGAACTTT GATTAAGCGT ACCAGTTATT TGAACCGTAG CGCGGGCAGG TTAACGAACC GAGCCATTCC TCGATAGAGT TCGGCAAAAC CTTCGTTTTG TCGAAC |
| res_site_III | Tn5044 | 982-1023 | + | 42 | ACTCGACTAC GTTAGTAATA GTTGAACTTT GATTAAGCGT AC |
| res_site_II | Tn5044 | 1025-1060 | + | 36 | AGTTATTTGA ACCGTAGCGC GGGCAGGTTA ACGAAC |
| res_site_I | Tn5044 | 1080-1107 | + | 28 | GTTCGGCAAA ACCTTCGTTT TGTCGAAC |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpR | Tn5044 | 41-967 | - | Accessory Gene | Resolvase |
| RHH_6 | Tn5044 | 1169-1408 | + | Passenger Gene | Antitoxin |
| PIN_3 | Tn5044 | 1408-1818 | + | Passenger Gene | Toxin |
| tnpA | Tn5044 | 1822-4815 | + | Transposase | |
| phospholipase | Tn5044 | 5049-5906 | - | Passenger Gene | Other |
| merD | Tn5044 | 6129-6488 | - | Passenger Gene | Heavy Metal Resistance |
| sigY | Tn5044 | 6485-7225 | - | Passenger Gene | Heavy Metal Resistance |
| merA | Tn5044 | 7335-9017 | - | Passenger Gene | Heavy Metal Resistance |
| merC | Tn5044 | 9053-9472 | - | Passenger Gene | Heavy Metal Resistance |
| merP | Tn5044 | 9502-9777 | - | Passenger Gene | Heavy Metal Resistance |
| merT | Tn5044 | 9791-10141 | - | Passenger Gene | Heavy Metal Resistance |
| merR | Tn5044 | 10213-10620 | + | Passenger Gene | Heavy Metal Resistance |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn5044 | 308 | 41-967 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MKIGYARVST RDQKADLQVD ALKQAGCERI YQDIASGAKS ARPELDKLLA NVRPGDAVVI WKLDRLGRSL KHLVELVGEL AERKVGLQSL NDPIDTTHAQ GRLVFNLFAS LAEFERELIR ERTHAGLSAA RARGRIGGRP KGLPAKAEAT AMAAETLYRE GRLSVSAIGE KLHISKSTLY SYLRHRGVDI GTYQKSAQPR GQQLNVASPV EPAVERVATV TLRLAVVNNS KFVRGRKRAT ENIERYCLEP YGMKRLETGH YELTIPHRSD DELDKTVHDL LTEISQEADM RNCFIEADAW EEGTERRW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | RHH_6 | RHH_6 | Tn5044 | 79 | 1169-1408 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antitoxin | ||||||||||
| Function: | Antitoxin | ||||||||||
| Sequence Family: | RHH_6 (Pfam:PF16762) | ||||||||||
| Protein Sequence: | MNTIRWNVAV SADTDQSLRM FLASQGGGRK GDLSRFIEEA VRAHILELSA EQAKAVNAHL SEAELTDAVD EALAWASKR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | PIN_3 | PIN_3 | Tn5044 | 136 | 1408-1818 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Toxin | ||||||||||
| Function: | Toxin | ||||||||||
| Sequence Family: | PIN_3 (Pfam:PF13470) | ||||||||||
| Target: | single stranded RNA | ||||||||||
| Comment: | Pfam PF13470.5 | ||||||||||
| Protein Sequence: | MRVVLDTNIL FSALISPHGA PDAIYRAWRA SRFEVVTSRM QLDEIRRASR YPKLQAILQP AKVGAMINNL QRAVVLERLT IEVEADDPDD SFLLAMALAG DADYLVTGDR RAGLLQRGHI ERTRIVTPAV FCAEVL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn5044 | 997 | 1822-4815 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPVGFLTQEQ RDAFGRYVDA PSREELERYF HLSDEDREAI QVLRGNHNRL GYAVMLTTVR FVGVLPDKPA AVPVEVRQVL CRQLAIADPD CLQRYSDHRR WIHAADIQER YGYRHFTDPG VGFRLSRWLY ALCWTGTDRP GVLFERATSW LFTQKVLLPG VSQLERFIAQ LRSRVEERLW YTLGRSVTEE QRQHLQDLLL VAEGNRSSRL DQLRSGPVMI SGPALVRALR RLDDVRGLGI TLPAAAHIPP SRIAALARFA NTAKVTAINR LPASRRLATL VAFAVCLEAS AHDDALEVLE ALLRDLFNNA EKADKKARLR SLKDLDRSAA TLATACKVVL DASISDDNVR ARLFNDLPRA TLEKALEEVN ALIRPADDVY FLALAARYRS VRRFLPNLLS HIRFGFSPAG KGVAASLDWL QLNLPRRKPE DDAPQEIVAK AWQNHITRED GSLDMGAYVF CTLDALRTAL RRRDVFVSPS WRYADPRIGL LGGAEWLAAR PIICRSLGLT IDAGTTLDAL SAELDATWQT VAARLPDNPA IQLSENTEGK TELSLGALDK LDEPSSLLQL RAAVADLMPR VDLPEILLEI AARTGFAEAF THVSERNARA DNLVTSLCAV LLGGACNTGL EPLIRADNLA LRRDRLSWVS QNYIRDDTLS AANAILVGAQ SQLELAQVWG GGEVASADGM RFVVPVRTVH AGPNPKYFGT GRGVTWYNLI SDQFSGLNAI TVPGTLRDSL VLLAVVLEQQ TELQPTQIMT DTGAYSDVVF GLFRLLGYHF SPRLADVGGT RFWRTRPDAD YGKLNGLARQ SVKLDLIAEH WDDLLRLAGS LKLGRVPATG IMRTLQTGDR PTRLAQALAE FGRIEKTLHT LTYIDNESKR RATLTQLNRG EGRHSLARAV FHGKRGELRQ RYREGQEDQL GALGLVVNII VLWNTLYMTA AVERLRQHGY PVLEEDLARL SPLIYEHINM LGRYSFAVPD EVARGELRPL RNPEDDL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | phospholipase | Phospholipase | Tn5044 | 285 | 5049-5906 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Protein Sequence: | MNVEASSAHT ALVLCGGGAR GAMQVGFYQA IIELGLSFDL VVGSSVGALN GTLIAAGMSP AELSELWQGL RKPDVVSWNW RGLLGETGGF YSFAPLRRLL HEALPVKRFE ELKHPLVITT TNLQSGQPCY HERHGVLIEP MIASMSLPGV FPPVWIAGDQ HVDGGVTDNV PFDKALDRGA HEILMISCTC CPRDSTALAS PLKILLRSFM IALDTKYLCS LQRHRARGIG IQLVRPHFQQ PGGLLDFNQT AALIEVGYRE TLAAFRNLRS NRVPPRSPLP EGVPS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD | MerD | Tn5044 | 119 | 6129-6488 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | second regulatory element | ||||||||||
| Protein Sequence: | MSSYKISKLA EQAGVSVHVV RDYLLRGLLH PVRRTDSGYG IFDAQSLNRL RFVRTAFEAG IDLRELARWC QSLDGGGGDI DECRAHLRSL IAARRETLVA LDRQLAAGLP HAEPESRHA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | sigY | SigY | Tn5044 | 246 | 6485-7225 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | RNA polymerase sigma factor-like protein | ||||||||||
| Protein Sequence: | MAPRMAFRAT VTTYGAAVQP DGLSAAALLL SRLRAGDREA FTALVREHHR ALRALAVCIV GEAWADDAVQ EAWLAAYRAL PRFEGRASLK TWLWAIVRNQ ALARLGRERR YVPWEAVFPG VDGGKSLRFD AAERGPTTPG PWHQEGPEAL LSSAESAQCL GRAIAALPGA QQRAYWLRDM EGMELDNIAV HLATSNGNVR VLLHRARSAL RRVLNAYQGQ GECRCISRQP PLIDPLRGWL SEGVQA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA | MerA | Tn5044 | 560 | 7335-9017 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MTTLQISGMT CASCADHVKD ALEKIPGVHQ ADVSYASGKA ELTVDKRVNH EQMQAAVEAL GYRAAFEDAT LQPRPGLLDQ APGWLSGGIG AGKEDVRLHV AVIGSGGAAM AAALKAVEGG ARVTLIERGI IGGTCVNVGC VPSKIMIRAA HVAHLRRESP FDGGLSATTP TVLRERLLAQ QQGRVDELRH AKYESILEST PAISVLRGTA RFQDGHTLSV ELAEGGERIV AFDRCLVATG ASAAVPSIPG LKDTPYWTSD EALVSDSIPQ RLAVIGASVV AVELAQAFAR LGSRVTILAR SSMFFREDPA IGAAVTEVFR MEGIEVLEQT QASQVSQPNG EFVLSTNHGE LRADQLLIAT GRLPNTQGMN LDGAGVTLDE RGGIQIDRGM RTSARDIYAA GDCTDQPQFV YVAAAAGTRA AINMTGGEAT LNLDVMPSVV FTDPQVATVG YSEAEAHNAG LETDSRTLTL DNVPRALANF DTRGSSSWCA EAGSGRLLGV QAVTPEAGEL IQSAVIAIRA RMTVQELADQ LFPYLTMVEG LKLAAQTFTK DVKQLSCCAG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merC | MerC | Tn5044 | 139 | 9053-9472 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | mercury transporter | ||||||||||
| Protein Sequence: | MGLITRIADK TGVVGSVVSA MGCAVCFPAM ASLGAAIGLG FLGQYEGLFI TTLLPLFAVV ALLANALGWF SHRQWQRSLL GITGPTIVLA ALYLFFGRWW TAGLFYTGLA LMLGEAIWDL LSPAHRRCAS NSCPPPEKH |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | Tn5044 | 91 | 9502-9777 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | Periplasmic mercury ion-binding protein | ||||||||||
| Protein Sequence: | MKKLFASLAL IAFVSPVWAA SQTVTLSVPG MTCAACPITV KKALSKVEGV SNIVVSFDTR EASVTFDDAK TNAQALTKAT ENAGYPSSVK Q |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | Tn5044 | 116 | 9791-10141 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | mercury transporter= | ||||||||||
| Protein Sequence: | MSEPSNGRAP LVAGGLAAIL ASTCCLGPLV LIALGFSGAW IGNLTVLEPY RPLFIGAALV ALFFAYRRIF RPLPACKPGE ACAIPQVRTT YKLIFWVVAA LVLVAFGFPY ILPLFY |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR | MerR | Tn5044 | 135 | 10213-10620 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MENILESLTI GTFAKAAGVN VETIRFYQRK GLLPEPDKPY GSIRRYGEAD VARVKFVKSA QRLGFSLDEV AGLLRLDDGA HCDEARVLAE QKLEDVRGKL ADLQRIESVL ARLVHDCCAS QPTITCPLIV SLHGE |
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1. Minakhina S, Kholodii G, Mindlin S, Yurieva O, Nikiforov V.
Tn5053 family transposons are res site hunters sensing plasmidal res sites occupied by cognate resolvases.. Mol Microbiol. 1999 Sep;33(5):1059-68. doi: 10.1046/j.1365-2958.1999.01548.x. PubMed ID:
10476039
.
2. Kholodii G, Yurieva O, Mindlin S, Gorlenko Z, Rybochkin V, Nikiforov V. Tn5044, a novel Tn3 family transposon coding for temperature-sensitive mercury resistance.. Res Microbiol. 2000 May;151(4):291-302. doi: 10.1016/s0923-2508(00)00149-2. PubMed ID: 10875286 .
3. Kholodii G, Bogdanova E. Tn5044-conferred mercury resistance depends on temperature: the complexity of the character of thermosensitivity.. Genetica. 2002 Jun;115(2):233-41. doi: 10.1023/a:1020185206563. PubMed ID: 12403178 .
2. Kholodii G, Yurieva O, Mindlin S, Gorlenko Z, Rybochkin V, Nikiforov V. Tn5044, a novel Tn3 family transposon coding for temperature-sensitive mercury resistance.. Res Microbiol. 2000 May;151(4):291-302. doi: 10.1016/s0923-2508(00)00149-2. PubMed ID: 10875286 .
3. Kholodii G, Bogdanova E. Tn5044-conferred mercury resistance depends on temperature: the complexity of the character of thermosensitivity.. Genetica. 2002 Jun;115(2):233-41. doi: 10.1023/a:1020185206563. PubMed ID: 12403178 .