Mobile element type:
Transposon
Name:
Tn502
Synonyms:
Accession:
Tn502-EU306743
Family:
Tn402
Group:
Tn5053
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Pseudomonas aeruginosa RM1
Date of Isolation:
1989
Country:
Molecular Source:
plasmid pVS6
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRt | 1-25 | + | 25 | TGTCATTTTC AGAAGACGAC TGCAC |
| repeat t1 | 9-27 | + | 19 | TCAGAAGACG ACTGCACCA |
| repeat t2 | 49-67 | - | 19 | TCAGGAGTCG CCTGCACAT |
| IR Tn21-like | 9548-9585 | - | 38 | GGGGATGTTT CAGAAAGCGG AAAATAAAGC ACGCTAAG |
| IRt | 9625-9649 | - | 25 | TGTCATTTTC AGAAGACGAT TGCAC |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| repeat t3 | Tn502 | 78-97 | + | 20 | TCAGAAGTCA TCTGCACCAA |
| r3 | Tn502 | 3931-3944 | + | 14 | GGGTTAAGTG ACAA |
| res | Tn502 | 3972-4006 | + | 35 | ACACTGTCAC ATAATCGAAC GTATATGTGA CAGGT |
| r2 | Tn502 | 3975-3988 | - | 14 | CGATTATGTG ACAG |
| r1 | Tn502 | 3991-4004 | + | 14 | CGTATATGTG ACAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tniA | Tn502 | 144-1826 | + | Transposase | |
| tniB | Tn502 | 1829-2737 | + | Accessory Gene | |
| tniQ | Tn502 | 2734-3951 | + | Accessory Gene | Target Site Selection |
| tniR | Tn502 | 4012-4626 | + | Accessory Gene | Resolvase |
| urf2 | Tn502 | 4637-5572 | - | Passenger Gene | Other |
| merE | Tn502 | 5623-5859 | - | Passenger Gene | Heavy Metal Resistance |
| merD | Tn502 | 5856-6221 | - | Passenger Gene | Heavy Metal Resistance |
| merA | Tn502 | 6239-7936 | - | Passenger Gene | Heavy Metal Resistance |
| merC | Tn502 | 7976-8338 | - | Passenger Gene | Heavy Metal Resistance |
| merP | Tn502 | 8384-8656 | - | Passenger Gene | Heavy Metal Resistance |
| merT | Tn502 | 8696-9046 | - | Passenger Gene | Heavy Metal Resistance |
| merR | Tn502 | 9118-9552 | + | Passenger Gene | Heavy Metal Resistance |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniA | TniA | Tn502 | 560 | 144-1826 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | homologous to TnsB of Tn7 | ||||||||||
| Protein Sequence: | MASDTSLIAE QGVATLPDAA WAQARQRAEI IGPLAALDVV GHEAADAAAH ALGLSRRQVY VLIRRARQGA GLVTDLARSR SGGGKGKGRL PESVERIIRE LLQKRFLTKQ KRSLAAFHRE VAQACKAQKL RAPARNTVAL RIAGLDPLKA TRRREGQDAS RSLQGVGGEP PAVTAPLEQV QIDHTVIDLI VVDERDRQPI GRPYLTIAID VFTRCVLGMV VTLEAPSSVS VGLCLVHVAC DKRPWLEGLN IEMEWPMSGK PRLLYLDNAA EFKSEALRRG CEQHGIRLDY RPLGQPHYGG IVERIIGTAM QMIHDELPGT TFSNPDQRGD YDSENKAALT LRELERWLTL AVGTYHGSVH NGLLQPPAAR WAEAIARTGV PTVITRTTAF LVDFLPIIRR TLTRTGFVID HIHYYADALK PWIARRDRLP AFLIRRDPRD ISRIWVLEPE GQHYLEIPYR TLSHPAVTLW EQRQALAKLR QQGREQVDES ALFRMIGQMR EIVSTAQKAT RKARRDADRR QHLKATAVLF KTTPPPDADM ADPQADNQPP AKPFDQIEEW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniB | TniB | Tn502 | 302 | 1829-2737 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | homologous to TnsC protein of Tn7 putative ATP-binding protein | ||||||||||
| Protein Sequence: | VEEYPIIDLS HLMPVAQGLA RLPADERIHR LRADRWIGYP RAVEALNRLE ALYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRTHPASSD ADQEHIPVLV VQMPSEPSVI RFYVALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRD AYLAIRSDDQ LENRFEPMML PVWEANDDCC SLLASFAASL PLRRPSSIAT LDMARYLLTR SEGTIGELAH LLMAAAVAAV ESGEEAINHR TLSMADYTGP SERRRQFERE LM |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniQ | TniQ | Tn502 | 405 | 2734-3951 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Target Site Selection | ||||||||||
| Protein Sequence: | MKPAPHWPLH PAPREGEALS SWLNRVALCY HMEVSELLEH DLGHGQVDDL DTAPPLALLA MLSQRSGIEP DRLRCMSFAG WVPWLLDSLD DQIPDALETY AFQLSVLLPK LRRRTRSITS WRAWLPSQPI HRACPLCLND PANQAVLLAW KLPLMLSCPL HGCWLESYWG VPGRFLGWEN ADTAPRTASD AIAVMDRRTW QALTTGHVEL PRRRIHAGLW FRLLRTLLDE LNTPLSTCGT CAGYLRQVWE GCGHPLRAGQ SLWRPYETLN PAVRLQMLEA AATAISLIEM RDISPPGEHA KLFWSEPQTG FTSGLSAKTP KPEPVDHWQR AVQAIDEAII EARHDPETAR SLFALASYGR RDPASLEQLR ATFAKEGIPP EFLSHYVPDG PFACLRQNDG LSDKF |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniR | TniR | Tn502 | 204 | 4012-4626 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | also called TniC | ||||||||||
| Protein Sequence: | MLIGYMRVSK ADGSQATDLQ RDALIAADVD PVHLYEDQAS GKREDRPGLV ACLKALRPGD TLVVWKLDRL GRDLRHLINT VHDLTGRGIG LKVLTGHGAA IDTTTAAGKL VFGIFAALAE FERELIAERT IAGLASARAR GRKGGRPFKM TAAKLRLAMA AMGQPETKVG DLCQELGITR QTLYRHISPK GELRPDGTKL LNRI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | urf2 | Urf2 | Tn502 | 311 | 4637-5572 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Sequence Family: | EAL (Pfam:PF00563)||DUF3330 (Pfam:PF11809) | ||||||||||
| Protein Sequence: | MRGQLELHYQ PIVDLRSDQI VGAEALLRWR HPRLGLLLPG QFLPLVESSG LMPEIGAWVL GAACRQMREW RMLAWQPFRL AVNVSASQVR PDFDGWVKGV LADAELPAES LEIELTESVA FGDPAIFPAL EALRQIGVRF AADDFGTGYS CLQHLKCCPI TTLKIDQSFV AGLADDHRDR TIVRSVIQLA HGLGMDVVAE GVETPTSLAL LRQAACDTGQ GFLFAKPMPA AAFAAFVSQW RGATMNANDP TATSCCVCCK EIPLDAAFTP EGVEYVEHFC GLECYQRFQA RAKAATETSA EQNTCDSPPS G |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merE | MerE | Tn502 | 78 | 5623-5859 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | Broad-spectrum mercury transporter | ||||||||||
| Protein Sequence: | MNGPERLPTK TRKPFTGYLW GALAVLTCPC HLPILAVVLA GTTAGAFIGE HWGIAALTLT GLFALSVTRL LRAFRGGS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD | MerD | Tn502 | 121 | 5856-6221 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | secondary regulatory protein | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MNAYTVSRLA LDAGVSVHIV RDYLLRGLLR PVACTPGGYG LFDEAALQRL CFVRSAFEAG IGLDALARLC RALDAADGGE AATQLAVLRQ FVEHRREALA DLEAQLATLP TEPAQHAESL P |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA | MerA | Tn502 | 565 | 6239-7936 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MTTLKITGMT CDSCAVHVKE ALEKVPGVQS AEVSYAKGSA RLAIEAGTLP DTLTAAVAGL GYRATLADAP VAPAGGGLLG KVREWLGSDD KAGGDSGKLH VAVIGSGGAA MAAALKAVEQ GARVTLIERG TIGGTCVNVG CVPSKIMIRA AHIAHLRRES PFDNGIQAVA PVIQRTALLA QQQARVDELR HAKYEGILEG NPAITVLHGA ARFKDNRNLV VQLNGGSERM VTFDRCLVAT GASPAVPPIP GLKDTPYWTS TEALVSETIP KRLAVIGSSV VALELAQAFA RLGAKVTILA RSTLFFREDP AIGEAVTAAF RAEGIEVREH TQASQVAYIN GERDGEFVLT TAHGELRADK LLVATGRAPN TRSLALDVAG VMVNKQGAIV IDQGMHTSSS NIYAAGDCTD QPQFVYVAAA AGTRAAINMT GGDATLNLTA MPAVVFTDPQ VATVGYSEAE AHHDGIETDS RTLTLDNVPR ALANFDTRGF IKLVVEEGSG RLIGVQAVAP EAGELIQTAA LAIRNRMTVQ ELADQLFPYL TMVEGLKLAA QTFNKDVKQL SCCAG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merC | MerC | Tn502 | 120 | 7976-8338 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | mercuric ion transport protein | ||||||||||
| Protein Sequence: | MGCAACFPAI ASLGAAIGLG FLQEYEGLFI SKLLPLFAVV ALLANALGWL SHRQWHRSLL GMIGPAIVFA GTVWLLGNWW AAHLVYTGLA LMVGVSIWDL VSPANRRCGP DGCELPTKQG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | Tn502 | 90 | 8384-8656 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercury transport | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | KKLFASLALA AIVAPVWAAT QTVTLSVPGM TCSACPITVK KAISKVEGVS KVDVTFETRE AVVTFDDAKT SVQKLTKATT DAGYPSSVKQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | Tn502 | 116 | 8696-9046 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | integral membrane protein | ||||||||||
| Protein Sequence: | MSEPQNGRGA LFAGGLAAIL ASTCCLGPLV LVALGFSGAW IGNLTVLEPY RPIFIGAALV ALFFAWRRIY RPTQACKPGE VCAIPQMRAT YKLIFWVVAV LVLVALGFPY VMPFFY |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR | MerR | Tn502 | 144 | 9118-9552 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | regulatory protein (repressor /inducer) | ||||||||||
| Protein Sequence: | MEKNLENLTI GVFAKAAGVN VETIRFYQRK GLLPEPDKPY GSIRRYGAAD VTRVRFVKSA QRLGFSLDEI AELLRLDDGT HCEEASSLAE HKLQDVREKM ADLARMEAVL SNLVCACHAR KGNVSCPLIA SLQGETSLAR SETP |
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1. Stanisich VA, Arwas R, Bennett PM, de la Cruz F.
Characterization of Pseudomonas mercury-resistance transposon Tn502, which has a preferred insertion site in RP1.. J Gen Microbiol. 1989 Nov;135(11):2909-15. doi: 10.1099/00221287-135-11-2909. PubMed ID:
2559144
.
2. Petrovski S, Stanisich VA. Tn502 and Tn512 are res site hunters that provide evidence of resolvase-independent transposition to random sites.. J Bacteriol. 2010 Apr;192(7):1865-74. doi: 10.1128/JB.01322-09. Epub 2010 Jan 29. PubMed ID: 20118251 .
2. Petrovski S, Stanisich VA. Tn502 and Tn512 are res site hunters that provide evidence of resolvase-independent transposition to random sites.. J Bacteriol. 2010 Apr;192(7):1865-74. doi: 10.1128/JB.01322-09. Epub 2010 Jan 29. PubMed ID: 20118251 .