Mobile element type:
Transposon
Name:
Tn501
Synonyms:
Accession:
Tn501-Z00027
Family:
Tn3
Group:
Tn21
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Pseudomonas aeruginosa
Date of Isolation:
1977
Country:
Molecular Source:
plasmidpVS1
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGGAACCG CAGAATTCGG AAAAAATCGT ACGCTAAG |
| IR | 81-118 | + | 38 | GGGGTCGTCT CAGAAAACGG AAAATAAAGC ACGCTAAG |
| IRR | 8318-8355 | - | 38 | GGGGGGCTCG CAGAATTCGG AAAAAATCGT ACGCTAAG |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | Tn501 | 4603-4729 | + | 127 | CTACCGTCAG ATTGAGGCAT ACCCTAACTG GATGTCAGGC AGGGCCGCGC CGCTTAGTCA GAATAGAGTC ATCTTTCGCA TTTTTGACAC ATGCCTGCGA AGGTCATAGA TTTCAGCCTG ACAGAAA |
| res_site_I | Tn501 | 4607-4637 | + | 31 | CGTCAGATTG AGGCATACCC TAACTGGATG T |
| res_site_II | Tn501 | 4662-4691 | + | 30 | AGAATAGAGT CATCTTTCGC ATTTTTGACA |
| res_site_III | Tn501 | 4694-4725 | + | 32 | TGCCTGCGAA GGTCATAGAT TTCAGCCTGA CA |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| merR | Tn501 | 114-548 | - | Passenger Gene | Heavy Metal Resistance |
| merT | Tn501 | 620-970 | + | Passenger Gene | Heavy Metal Resistance |
| merP | Tn501 | 983-1258 | + | Passenger Gene | Heavy Metal Resistance |
| merA | Tn501 | 1330-3015 | + | Passenger Gene | Heavy Metal Resistance |
| merD | Tn501 | 3033-3398 | + | Passenger Gene | Heavy Metal Resistance |
| merE | Tn501 | 3395-3631 | + | Passenger Gene | Heavy Metal Resistance |
| urfM | Tn501 | 3628-4617 | + | Passenger Gene | Other |
| tnpR | Tn501 | 4792-5352 | + | Accessory Gene | Resolvase |
| tnpA | Tn501 | 5356-8322 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR | MerR | Tn501 | 144 | 114-548 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | activator-repressor of mer operon | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MENNLENLTI GVFAKAAGVN VETIRFYQRK GLLLEPDKPY GSIRRYGEAD VTRVRFVKSA QRLGFSLDEI AELLRLEDGT HCEEASSLAE HKLKDVREKM ADLARMEAVL SELVCACHAR RGNVSCPLIA SLQGGASLAG SAMP |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | Tn501 | 116 | 620-970 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | cytosolic mercuric ion transport protein | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MSEPKTGRGA LFTGGLAAIL ASACCLGPLV LIALGFSGAW IGNLAVLEPY RPIFIGVALV ALFFAWRRIY RQAAACKPGE VCAIPQVRAT YKLIFWIVAA LVLVALGFPY VMPFFY |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | Tn501 | 91 | 983-1258 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercury transport | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MKKLFASLAL AAVVAPVWAA TQTVTLSVPG MTCSACPITV KKAISEVEGV SKVDVTFETR QAVVTFDDAK TSVQKLTKAT ADAGYPSSVK Q |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA | MerA | Tn501 | 561 | 1330-3015 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercuric ion reductase | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MTHLKITGMT CDSCAAHVKE ALEKVPGVQS ALVSYPKGTA QLAIVPGTSP DALTAAVAGL GYKATLADAP LADNRVGLLD KVRGWMAAAE KHSGNEPPVQ VAVIGSGGAA MAAALKAVEQ GAQVTLIERG TIGGTCVNVG CVPSKIMIRA AHIAHLRRES PFDGGIAATV PTIDRSKLLA QQQARVDELR HAKYEGILGG NPAITVVHGE ARFKDDQSLT VRLNEGGERV VMFDRCLVAT GASPAVPPIP GLKESPYWTS TEALASDTIP ERLAVIGSSV VALELAQAFA RLGSKVTVLA RNTLFFREDP AIGEAVTAAF RAEGIEVLEH TQASQVAHMD GEFVLTTTHG ELRADKLLVA TGRTPNTRSL ALDAAGVTVN AQGAIVIDQG MRTSNPNIYA AGDCTDQPQF VYVAAAAGTR AAINMTGGDA ALDLTAMPAV VFTDPQVATV GYSEAEAHHD GIETDSRTLT LDNVPRALAN FDTRGFIKLV IEEGSHRLIG VQAVAPEAGE LIQTAALAIR NRMTVQELAD QLFPYLTMVE GLKLAAQTFN KDVKQLSCCA G |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD | MerD | Tn501 | 121 | 3033-3398 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | secondary regulatory protein | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MNAYPVSRLA LDAGVSVHIV RDYLLRGLLR PVACTPGGYG LFDDAALQRL CFVRAAFEAG IGLDALARLC RALDAADGDE AAAQLALLRQ FVERRREALA DLEVQLATLP TEPAQHAESL P |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merE | MerE | Tn501 | 78 | 3395-3631 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercury transport | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MNNPERLPSE THKPITGYLW GGLAVLTCPC HLPILAVVLA GTTAGAFLGE HWVIAALGLT GLFLLSLSRA LRAFRERE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | urfM | UrfM | Tn501 | 329 | 3628-4617 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Function: | possible diguanylate phosphodiesterase | ||||||||||
| Sequence Family: | EAL (Pfam:PF00563)||DUF3330 (Pfam:PF11809) | ||||||||||
| Comment: | similar to UrfM from E.coli | ||||||||||
| Protein Sequence: | MSAFRPDGWT TPELAQAVER GQLELHYQPV VDLRSGGIVG AEALLRWRHP TLGLLPPGQF LPVVESSGLM PEIGAWVLGE ACRQMRDWRM LAWRPFRLAV NASASQVGPD FDGWVKGVLA DAELPAEYLE IELTESVAFG DPAIFPALDA LRQIGVRFAA DDFGTGYSCL QHLKCCPIST LKIDQSFVAG LANDRRDQTI VHTVIQLAHG LGMDVVAEGV ETSASLDLLR QADCDTGQGF LFAKPMPAAA FAVFVSQWRG ATMNASDSTT TSCCVCCKEI PLDAAFTPEG AEYVEHFCGL ECYQRFEARA KTGNETDADP NACDSLPSD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn501 | 186 | 4792-5352 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | resolvase||serine site-specific recombinase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MQGHRIGYVR VSSFDQNPER QLEQTQVSKV FTDKASGKDT QRPQLEALLS FVREGDTVVV HSMDRLARNL DDLRRLVQKL TQRGVRIEFL KEGLVFTGED SPMANLMLSV MGAFAEFERA LIRERQREGI TLAKQRGAYR GRKKALSDEQ AATLRQRATA GEPKAQLARE FNISRETLYQ YLRTDD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn501 | 988 | 5356-8322 | + |
|---|
| Class: | Transposase | ||||||||||
| Function: | transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPRRLILSAT ERGTLLALPE SQDDLIRYYT FNDSDLSLIR QRRGDANRLG FAVQLCLLRY PGYALGTDSE LPEPVILWVA KQVQTDPASW TKYGERDVTR REHAQELRTY LQLAPFGLSD FRALVRELTE LAQQTDKGLL LAGQALESLR QKRRILPALS VIDRACSEAI ARANRRVYRA LVEPLTDSHR AKLDELLKLK AGSSITWLTW LRQAPLKPNS RHMLEHIERL KTFQLVDLPE VLGRHIHQNR LLKLAREGGQ MTPKDLGKFE PQRRYATLAA VVLESTATVI DELVDLHDRI LVKLFSGAKH KHQQQFQKQG KAINDKVRLY SKIGQALLEA KEAGSDPYAA IEAVIPWDEF TESVSEAELL ARPEGFDHLH LVGENFATLR RYTPALLEVL ELRAAPAAQG VLAAVQTLRE MNADNLRKVP ADAPTAFIKP RWKPLVITPE GLDRRFYEIC ALSELKNALR SGDIWVKGSR QFRDFDDYLL PAEKFAALKR EQALPLAINP NSDQYLEERL QLLDEQLATV ARLAKDNELP DAILTESGLK ITPLDAAVPD RAQALIDQTS QLLPRIKITE LLMDVDDWTG FSRHFTHLKD GAEAKDRTLL LSAILGDAIN LGLTKMAESS PGLTYAKLSW LQAWHIRDET YSAALAELVN HQYQHAFAAH WGDGTTSSSD GQRFRAGGRG ESTGHVNPKY GSEPGRLFYT HISDQYAPFS TRVVNVGVRD STYVLDGLLY HESDLRIEEH YTDTAGFTDH VFALMHLLGF RFAPRIRDLG ETKLYVPQGV QTYPTLRPLI GGTLNIKHVR AHWDDILRLA SSIKQGTVTA SLMLRKLGSY PRQNGLAVAL RELGRIERTL FILDWLQSVE LRRRVHAGLN KGEARNSLAR AVFFNRLGEI RDRSFEQQRY RASGLNLVTA AIVLWNTVYL ERATQGLVEA GKPVDGELLQ FLSPLGWEHI NLTGDYVWRQ SRRLEDGKFR PLRMPGKP |
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1. Diver WP, Grinsted J, Fritzinger DC, Brown NL, Altenbuchner J, Rogowsky P, Schmitt R.
DNA sequences of and complementation by the tnpR genes of Tn21, Tn501 and Tn1721.. Mol Gen Genet. 1983;191(2):189-93. doi: 10.1007/BF00334812. PubMed ID:
6312271
.
2. Brown NL, Ford SJ, Pridmore RD, Fritzinger DC. Nucleotide sequence of a gene from the Pseudomonas transposon Tn501 encoding mercuric reductase.. Biochemistry. 1983 Aug 16;22(17):4089-95. doi: 10.1021/bi00286a015. PubMed ID: 6311258 .
3. Misra TK, Brown NL, Fritzinger DC, Pridmore RD, Barnes WM, Haberstroh L, Silver S. Mercuric ion-resistance operons of plasmid R100 and transposon Tn501: the beginning of the operon including the regulatory region and the first two structural genes.. Proc Natl Acad Sci U S A. 1984 Oct;81(19):5975-9. doi: 10.1073/pnas.81.19.5975. PubMed ID: 6091128 .
4. Brown NL, Winnie JN, Fritzinger D, Pridmore RD. The nucleotide sequence of the tnpA gene completes the sequence of the Pseudomonas transposon Tn501.. Nucleic Acids Res. 1985 Aug 12;13(15):5657-69. doi: 10.1093/nar/13.15.5657. PubMed ID: 2994007 .
5. Brown NL, Misra TK, Winnie JN, Schmidt A, Seiff M, Silver S. The nucleotide sequence of the mercuric resistance operons of plasmid R100 and transposon Tn501: further evidence for mer genes which enhance the activity of the mercuric ion detoxification system.. Mol Gen Genet. 1986 Jan;202(1):143-51. doi: 10.1007/BF00330531. PubMed ID: 3007931 .
2. Brown NL, Ford SJ, Pridmore RD, Fritzinger DC. Nucleotide sequence of a gene from the Pseudomonas transposon Tn501 encoding mercuric reductase.. Biochemistry. 1983 Aug 16;22(17):4089-95. doi: 10.1021/bi00286a015. PubMed ID: 6311258 .
3. Misra TK, Brown NL, Fritzinger DC, Pridmore RD, Barnes WM, Haberstroh L, Silver S. Mercuric ion-resistance operons of plasmid R100 and transposon Tn501: the beginning of the operon including the regulatory region and the first two structural genes.. Proc Natl Acad Sci U S A. 1984 Oct;81(19):5975-9. doi: 10.1073/pnas.81.19.5975. PubMed ID: 6091128 .
4. Brown NL, Winnie JN, Fritzinger D, Pridmore RD. The nucleotide sequence of the tnpA gene completes the sequence of the Pseudomonas transposon Tn501.. Nucleic Acids Res. 1985 Aug 12;13(15):5657-69. doi: 10.1093/nar/13.15.5657. PubMed ID: 2994007 .
5. Brown NL, Misra TK, Winnie JN, Schmidt A, Seiff M, Silver S. The nucleotide sequence of the mercuric resistance operons of plasmid R100 and transposon Tn501: further evidence for mer genes which enhance the activity of the mercuric ion detoxification system.. Mol Gen Genet. 1986 Jan;202(1):143-51. doi: 10.1007/BF00330531. PubMed ID: 3007931 .