Mobile element type:
Transposon
Name:
Tn5
Synonyms:
Accession:
Tn5-U00004.1
Family:
Compound Transposon
Group:
IS50
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Pseudomonas aeruginosa
Date of Isolation:
1975
Country:
Molecular Source:
plasmid JR67
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnp_truncated | IS50L | 92-1444 | + | Transposase | |
| inh_truncated | IS50L | 257-1444 | + | Accessory Gene | Inhibitor |
| APH(3')-IIa (ARO:3002644) | Tn5 | 1551-2345 | + | Passenger Gene | Antibiotic Resistance |
| BRP(MBL) (ARO:3001205) | Tn5 | 2366-2746 | + | Passenger Gene | Antibiotic Resistance |
| APH(6)-Ic (ARO:3002659) | Tn5 | 2785-3585 | + | Passenger Gene | Antibiotic Resistance |
| tnp | IS50R | 4297-5727 | - | Transposase | |
| inh | IS50R | 4298-5562 | - | Accessory Gene | Inhibitor |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp_truncated | Tnp_truncated | IS50L | 450 | 92-1444 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | truncated to 421 amino acids by ochre mutation in C-terminal region which creates a promoter for aphA2 expression||non-functional | ||||||||||
| Protein Sequence: | MITSALHRAA DWAKSVFSSA ALGDPRRTAR LVNVAAQLAK YSGKSITISS EGSEAMQEGA YRFYRNPNVS AEAIRKAGAM QTVKLAQEFP ELLAIEDTTS LSYRHQVAEE LGKLGSIQDK SRGWWVHSVL LLEATTFRTV GLLHQEWWMR PDDPADADEK ESGKWLAAAA TSRLRMGSMM SNVIAVCDRE ADIHAYLQDR LAHNERFVVR SKHPRKDVES GLYLIDHLKN QPELGGYQIS IPQKGVVDKR GKRKNRPARK ASLSLRSGRI TLKQGNITLN AVLAEEINPP KGETPLKWLL LTGEPVESLA QALRVIDIYT HRWRIEEFHK AWKTGAGAER QRMEEPDNLE RMVSILSFVA VRLLQLRESF TLPQALRAQG LLKEAEHVES QSAETVLTPD ECQLLGYLDK GKRKRKEKAG SLQWAYMAIA RLGGFMDSKR TGIASWGALW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | inh_truncated | Inh_truncated | IS50L | 395 | 257-1444 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Inhibitor | ||||||||||
| Comment: | truncated by ochre mutation in C-terminal end | ||||||||||
| Protein Sequence: | MQEGAYRFYR NPNVSAEAIR KAGAMQTVKL AQEFPELLAI EDTTSLSYRH QVAEELGKLG SIQDKSRGWW VHSVLLLEAT TFRTVGLLHQ EWWMRPDDPA DADEKESGKW LAAAATSRLR MGSMMSNVIA VCDREADIHA YLQDRLAHNE RFVVRSKHPR KDVESGLYLI DHLKNQPELG GYQISIPQKG VVDKRGKRKN RPARKASLSL RSGRITLKQG NITLNAVLAE EINPPKGETP LKWLLLTGEP VESLAQALRV IDIYTHRWRI EEFHKAWKTG AGAERQRMEE PDNLERMVSI LSFVAVRLLQ LRESFTLPQA LRAQGLLKEA EHVESQSAET VLTPDECQLL GYLDKGKRKR KEKAGSLQWA YMAIARLGGF MDSKRTGIAS WGALW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | APH(3')-IIa (ARO:3002644) | APH(3')-IIa | Tn5 | 264 | 1551-2345 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | APH(3') (ARO:3000126) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Protein Sequence: | MIEQDGLHAG SPAAWVERLF GYDWAQQTIG CSDAAVFRLS AQGRPVLFVK TDLSGALNEL QDEAARLSWL ATTGVPCAAV LDVVTEAGRD WLLLGEVPGQ DLLSSHLAPA EKVSIMADAM RRLHTLDPAT CPFDHQAKHR IERARTRMEA GLVDQDDLDE EHQGLAPAEL FARLKARMPD GEDLVVTHGD ACLPNIMVEN GRFSGFIDCG RLGVADRYQD IALATRDIAE ELGGEWADRF LVLYGIAAPD SQRIAFYRLL DEFF |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | BRP(MBL) (ARO:3001205) | BRP(MBL) | Tn5 | 126 | 2366-2746 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | Bleomycin resistant protein (ARO:3004256) | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081) | ||||||||||
| Comment: | loose match 57% to reference sequence for ARO:3001205 (bitscore: 142)||Synonyms: blmS, ble, BRP, blmA, BLMT | ||||||||||
| Protein Sequence: | MTDQATPNLP SRDFDSTAAF YERLGFGIVF RDAGWMILQR GDLMLEFFAH PGLDPLASWF SCCLRLDDLA EFYRQCKSVG IQETSSGYPR IHAPELQEWG GTMAALVDPD GTLLRLIQNE LLAGIS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | APH(6)-Ic (ARO:3002659) | APH(6)-Ic | Tn5 | 266 | 2785-3585 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | APH(6) (ARO:3000151) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | perfect match to ARO:3002659 reference sequence||Synonym: str | ||||||||||
| Protein Sequence: | MERWRLLRDG ELLTTHSSWI LPVRQGDMPA MLKVARIPDE EAGYRLLTWW DGQGAARVFA SAAGALLMER ASGAGDLAQI AWSGQDDEAC RILCDTAARL HAPRSGPPPD LHPLQEWFQP LFRLAAEHAA LAPAASVARQ LLAAPREVCP LHGDLHHENV LDFGDRGWLA IDPHGLLGER TFDYANIFTN PDLSDPGRPL AILPGRLEAR LSIVVATTGF EPERLLRWII AWTGLSAAWF IGDGDGEGEG AAIDLAVNAM ARRLLD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | IS50R | 476 | 4297-5727 | - |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MITSALHRAA DWAKSVFSSA ALGDPRRTAR LVNVAAQLAK YSGKSITISS EGSEAMQEGA YRFYRNPNVS AEAIRKAGAM QTVKLAQEFP ELLAIEDTTS LSYRHQVAEE LGKLGSIQDK SRGWWVHSVL LLEATTFRTV GLLHQEWWMR PDDPADADEK ESGKWLAAAA TSRLRMGSMM SNVIAVCDRE ADIHAYLQDR LAHNERFVVR SKHPRKDVES GLYLIDHLKN QPELGGYQIS IPQKGVVDKR GKRKNRPARK ASLSLRSGRI TLKQGNITLN AVLAEEINPP KGETPLKWLL LTGEPVESLA QALRVIDIYT HRWRIEEFHK AWKTGAGAER QRMEEPDNLE RMVSILSFVA VRLLQLRESF TLPQALRAQG LLKEAEHVES QSAETVLTPD ECQLLGYLDK GKRKRKEKAG SLQWAYMAIA RLGGFMDSKR TGIASWGALW EGWEALQSKL DGFLAAKDLM AQGIKI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | inh | Inh | IS50R | 421 | 4298-5562 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Inhibitor | ||||||||||
| Function: | Inhibitor of transposition | ||||||||||
| Comment: | has an internal start in the transposasegene from an internal promoter | ||||||||||
| Protein Sequence: | MQEGAYRFYR NPNVSAEAIR KAGAMQTVKL AQEFPELLAI EDTTSLSYRH QVAEELGKLG SIQDKSRGWW VHSVLLLEAT TFRTVGLLHQ EWWMRPDDPA DADEKESGKW LAAAATSRLR MGSMMSNVIA VCDREADIHA YLQDRLAHNE RFVVRSKHPR KDVESGLYLI DHLKNQPELG GYQISIPQKG VVDKRGKRKN RPARKASLSL RSGRITLKQG NITLNAVLAE EINPPKGETP LKWLLLTGEP VESLAQALRV IDIYTHRWRI EEFHKAWKTG AGAERQRMEE PDNLERMVSI LSFVAVRLLQ LRESFTLPQA LRAQGLLKEA EHVESQSAET VLTPDECQLL GYLDKGKRKR KEKAGSLQWA YMAIARLGGF MDSKRTGIAS WGALWEGWEA LQSKLDGFLA AKDLMAQGIK I |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| IS50L-U00004.1 | IS50L | insertion sequence | 1-1533 | + | 1533 |
| IS50R-U00004.1 | IS50R | insertion sequence | 4286-5818 | + | 1533 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRL | IS50L | 1-9 | + | 9 | CTGACTCTT |
| IRR | IS50L | 1525-1533 | - | 9 | CTGTCTCTT |
| IRR | IS50R | 4286-4294 | + | 9 | CTGTCTCTT |
| IRL | IS50R | 5810-5818 | - | 9 | CTGACTCTT |
1. Berg DE.
Julian Davies and the discovery of kanamycin resistance transposon Tn5.. J Antibiot (Tokyo). 2017 Apr;70(4):339-346. doi: 10.1038/ja.2016.120. Epub 2016 Oct 12. PubMed ID:
27731334
.
2. Berg DE, Davies J, Allet B, Rochaix JD. Transposition of R factor genes to bacteriophage lambda.. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3628-32. doi: 10.1073/pnas.72.9.3628. PubMed ID: 1059152 .
3. Auerswald EA, Ludwig G, Schaller H. Structural analysis of Tn5.. Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:107-13. doi: 10.1101/sqb.1981.045.01.019. PubMed ID: 6271452 .
4. Beck E, Ludwig G, Auerswald EA, Reiss B, Schaller H. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.. Gene. 1982 Oct;19(3):327-36. doi: 10.1016/0378-1119(82)90023-3. PubMed ID: 6295884 .
5. Mazodier P, Cossart P, Giraud E, Gasser F. Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes.. Nucleic Acids Res. 1985 Jan 11;13(1):195-205. doi: 10.1093/nar/13.1.195. PubMed ID: 3889831 .
6. Johnson RC, Yin JC, Reznikoff WS. Control of Tn5 transposition in Escherichia coli is mediated by protein from the right repeat.. Cell. 1982 Oct;30(3):873-82. doi: 10.1016/0092-8674(82)90292-6. PubMed ID: 6291786 .
7. Rothstein SJ, Jorgensen RA, Yin JC, Yong-di Z, Johnson RC, Reznikoff WS. Genetic organization of Tn5.. Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:99-105. doi: 10.1101/sqb.1981.045.01.018. PubMed ID: 6271497 .
8. Rothstein SJ, Reznikoff WS. The functional differences in the inverted repeats of Tn5 are caused by a single base pair nonhomology.. Cell. 1981 Jan;23(1):191-9. doi: 10.1016/0092-8674(81)90284-1. PubMed ID: 6260374 .
9. Johnson RC, Reznikoff WS. DNA sequences at the ends of transposon Tn5 required for transposition.. Nature. 1983 Jul 21-27;304(5923):280-2. doi: 10.1038/304280a0. PubMed ID: 6306482 .
2. Berg DE, Davies J, Allet B, Rochaix JD. Transposition of R factor genes to bacteriophage lambda.. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3628-32. doi: 10.1073/pnas.72.9.3628. PubMed ID: 1059152 .
3. Auerswald EA, Ludwig G, Schaller H. Structural analysis of Tn5.. Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:107-13. doi: 10.1101/sqb.1981.045.01.019. PubMed ID: 6271452 .
4. Beck E, Ludwig G, Auerswald EA, Reiss B, Schaller H. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.. Gene. 1982 Oct;19(3):327-36. doi: 10.1016/0378-1119(82)90023-3. PubMed ID: 6295884 .
5. Mazodier P, Cossart P, Giraud E, Gasser F. Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes.. Nucleic Acids Res. 1985 Jan 11;13(1):195-205. doi: 10.1093/nar/13.1.195. PubMed ID: 3889831 .
6. Johnson RC, Yin JC, Reznikoff WS. Control of Tn5 transposition in Escherichia coli is mediated by protein from the right repeat.. Cell. 1982 Oct;30(3):873-82. doi: 10.1016/0092-8674(82)90292-6. PubMed ID: 6291786 .
7. Rothstein SJ, Jorgensen RA, Yin JC, Yong-di Z, Johnson RC, Reznikoff WS. Genetic organization of Tn5.. Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:99-105. doi: 10.1101/sqb.1981.045.01.018. PubMed ID: 6271497 .
8. Rothstein SJ, Reznikoff WS. The functional differences in the inverted repeats of Tn5 are caused by a single base pair nonhomology.. Cell. 1981 Jan;23(1):191-9. doi: 10.1016/0092-8674(81)90284-1. PubMed ID: 6260374 .
9. Johnson RC, Reznikoff WS. DNA sequences at the ends of transposon Tn5 required for transposition.. Nature. 1983 Jul 21-27;304(5923):280-2. doi: 10.1038/304280a0. PubMed ID: 6306482 .