Mobile element type:
Transposon
Name:
Tn4661
Synonyms:
Accession:
Tn4661-AB375440.1
Family:
Tn3
Group:
Tn4651
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Pseudomonas aeruginosa
Date of Isolation:
2013
Country:
Molecular Source:
plasmid Rms148
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-47 | + | 47 | GGGGTCATGC CGAGATAAGG GGAAAATTCA TTCATTTGGA ATGTAAG |
| IRR | 12659-12705 | - | 47 | GGGGGCATGC CGAGATAAGG GGGAAATTCA TTCATTTGGT CTGTAAG |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | Tn4661 | 10721-10856 | + | 136 | GTGTACTGGG AAACGACCGC GAAGCAGCCG TTTTTGCCAA TCATATTGAA TAATCATCGG TTATTTTATC TGAAACAAGC CTTCAAATCC TGCTTTACTT CATGACAATT TAGTATGTAA ATACATGGTA TGTAAT |
| res_core_IRL | Tn4661 | 10761-10773 | + | 13 | TCATATTGAA TAA |
| res_core_IRR | Tn4661 | 10781-10793 | - | 13 | TCAGATAAAA TAA |
| res_acc_IR1 | Tn4661 | 10831-10839 | + | 9 | TAGTATGTA |
| res_acc_IR2 | Tn4661 | 10842-10850 | - | 9 | TACCATGTA |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpA | Tn4661 | 138-3152 | + | Transposase | |
| tnpC | Tn4661 | 3149-3511 | + | Accessory Gene | Inhibitor/Regulator |
| GNAT_fam | Tn4661 | 3681-4145 | - | Passenger Gene | Antibiotic Resistance |
| eamA family | Tn4661 | 4153-5058 | - | Passenger Gene | Other |
| WP_003111046.1 | Tn4661 | 5055-5783 | - | Passenger Gene | Hypothetical |
| homospermidine synthase | Tn4661 | 5799-7208 | - | Passenger Gene | Other |
| tcmP | Tn4661 | 7620-8936 | - | Passenger Gene | Other |
| Gp37Gp68 family | Tn4661 | 8958-9701 | - | Passenger Gene | Other |
| tnpS | Tn4661 | 9731-10702 | - | Accessory Gene | Resolvase |
| tnpT | Tn4661 | 10883-11884 | + | Accessory Gene | Resolvase |
| WP_031628629.1 | Tn4661 | 12177-12479 | - | Passenger Gene | Hypothetical |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn4661 | 1004 | 138-3152 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MASLERTAYP VLSRSYSRAE LQREFSFSEE EVTWVKSRSN AAFRLNLAVL FKTFQVLRYF PEIAEVPEPV VDFIRTQIGL RSKVALSPYK WMQLYRHMSA VREKLGVRPF YGSDGQDIAS THAQLMAPLL EQRADIINAI IDELLRQNYE LPAYSTLNDL AEAARAEAQE KIFNLVVARA PIKVIYKLRD LLDTDFGRRQ SDLNTLKQAP KKPSRKHLEV LIDHLAWLES FGKLDAIFDG IVDTKIRHFA AQAAASDVSE LKDCSLPKRY TLMLALIYRM RVRTRDHLAE MFIRRISTIH KRAKEELEQI QARQRQKLEQ LAATLDGVVQ ILVQEPDDQE AGSLIREYLS PDGNMDRLRE VCAEVQATGG NNYLPLIWRH FKSHRSLLFR LSHLLQLEPT TQDRSLIQAL QLIQDSENLH REWIDEHVDL SFASDRWVKI VRRPTSEGPP TNRRYLEVCV FSYLASELRS GDLCVQGSES FADYRKQLLP WEECLQRLPA YCEKVGLPTT AKEFVASLKS QLEETAQQLD DKFPSCRGDV SINDAGEPVL RRVMARDIPP SAISLQTALM QRMPARHVLD IMANIEHWIQ FTRHFGPMSG NEPKLKEPAE RYLMTIFAMG CNLGPSQAAR HLAGNVTPHM LSYTNRRHLS LEKLDKANRE LVELYLQLDL PKLWGDGKAV AADGTQFDFY DDNLLAGYHF RYRKMGAVAY RHVANNYIAV FQHFIPPGIW EAIYVIEGLL KVSLSVEPDT VYSDTQGQSA TVFAFTHLLG INLMPRIRNW RDLVMCRPDR GVSYKHINRL FTDTADWHLI ETHWQDLMQI ALSIQAGKIS SPMLLRKLGS YSRRNKLYLA AQTLGSVIRT IFLLNWIGSR ELRQEVTANT NKIESYNGFS KWLSFGGDVI AENDPDEQQK RLRYNDMVAS SVILQNTVDM MRILQKLARE GWQFTDEDVS FLSPYLTSNV KRFGEFNLKL NRPPEPWIKD SVFQQAAGSL RVNSTSKAAA EEAI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpC | TnpC | Tn4661 | 120 | 3149-3511 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Inhibitor/Regulator | ||||||||||
| Sequence Family: | Tn3_family | ||||||||||
| Comment: | putative tnpA repressor | ||||||||||
| Protein Sequence: | MIEIASASFR VTPYGEVDIA ALDRMRAGFD TSQLLRLVEG LDACLSEMGG ITALRDELLK LHAMALTIVE GTAPAAPTEN ACIWAEAESV QLGLEALATW IRSAQGFLTP LTSLEPEHQQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | GNAT_fam | GNAT_fam | Tn4661 | 154 | 3681-4145 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Sequence Family: | Acetyltransf_1 (Pfam:PF00583) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | putative acetyltransferase | ||||||||||
| Protein Sequence: | MSKNSNIKLI SASIDDELLL VELMKKYHLY DGIEMTDEDR LAALRPLLSS SEKGNVWIIC LGSKQIGYIA TCNCYSIEFR GIEVWIDEFY IDEQCRGQGF GSEILEKVKS FLKSQGAAIV HLEVDENNPK AVSLYKKSGF EFRERFVMMS HSLK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | eamA family | EamA family | Tn4661 | 301 | 4153-5058 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | transmembrane transporter | ||||||||||
| Protein Sequence: | MTWLVMALMA PLLWSLSNIL DKLAVIHHAN HWTTFIFLLS IGNLLYSALI FLLFTPSDFD TTSALLNLLS GLCIFGMYFL YAMPLKSVDI STAIALHQLT PLLVLVVSIL LLGYSPTGIE LLGIALVFVG VYFAVAIGKG RPGSISLSAV LLLIGSAVFG AVATLLYDHL LLSHSLPSVL MFNYAGYGLG GTLMLLVPKW RHSIAKDVSE MPPKGWAVYA LSNLFDAGGY ALFIGALSAG GNVAVVSVLV SLHPVYVLAL SHWLAIVDPK ILSENFSSSD AYKRMSAIVV IVAGVIMMTK I |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | WP_003111046.1 | WP_003111046.1 | Tn4661 | 242 | 5055-5783 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Protein Sequence: | VSNVKSGIGL KSYEWSSLEL LKGLSIEQIK SNPSKLEERR PFFWHDMSSE FDSINFLRYL FGRRDIQFSN EFIEFVCLWH LDEQNHYRGL RKINSVLYSM PEDMIDREIR SNSPDFSHIE DFMKDEFTIL LSIAFDEVTS TRAYKQDVSF FDSFENESLS TWIRYAARDE AAHYGNAMKI LRLNHSHRFD EVEAILDKIV EFETSESFDY QRTFIFDHDT DDFSHVLLKD SRDTILEVLR GK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | homospermidine synthase | Homospermidine synthase | Tn4661 | 469 | 5799-7208 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Protein Sequence: | MDFSINPPQR IVFVGLGTIA QSFLPLLSKV HDLSTLEIYA IDPKTPPLIE YFANSFGLKF INSAIDQINY RDILVPILGE GTVLINLSTD VSSLALIELC RSAGALYLDT CIEPWKGGYD DPTIPLHKRT NYHLREQMLS LKKRLGSGVT ALVAHGANPG LVSHFVKRAL LDLAEEILGD CKKPSNKEQW AILSQRLGVK VIHVAEYDSQ ISQKSRERGE FVNTWSVHGF ISESQQPAEL GWGSHERSLP TDASMHTDGC GAAIYIEKPG ASVRVKTWTP FNGPSLGYLV THHEAISIAD FLTLRTADET YRPTVHYAYR PSDEAILSVH EWFGNDCMTP EKTKVLRPGD ILSGSDYLGV LLMGHEKSSY WYGSILSIEK AKELATLNTA TTLQVAAGVL SGYLWILSHP SAGIIEAEDM DHEVALSYIS QYLGELKGVY SDWNPTKNNP GTFSAIDSDS PWLFSNFVL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tcmP | TcmP | Tn4661 | 438 | 7620-8936 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | three-Cys-motif partner protein TcmP | ||||||||||
| Protein Sequence: | MAKDDEKYRW DWATQTFPTI DPHSKIKHQI IDDYIQAYID VLMRNQQMPL LGLSIVDGFS GGGIYNDSAG GHHFGSPVIA LEAIQESEVR NNVGRIKYRP ICSQHHFVDV KPKNTACLHA VLASRGHQAR IGQDIHLHTA EFTKALPAIA QSLKAFGKSE RVLFLLDQYG YGDVPFGQIK WIFQHLSNAE VLLTFNVDFL ITYLADRHAN RKAIASIGLE QYVPWEQLRH LKAHSSQDWQ YLIQRYLSDG IKQESGAQFM TVFFIRPHGT NPMAYWFIHL ANSYRANDVM KAIHWKYGNN FSHLLSPSCF FGYDANRDVG VTGQPDLLCE GEHQFDQVTD TRIRGELSEL LPKQVFEVER RPFIQLMTEL TNFTMADESR VKLALDVAVA NGDIQAVDKE GKSRRRKGTS IKSTDILIAP PQKTFFFFPQ QKKTSRDT |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | Gp37Gp68 family | Gp37Gp68 family | Tn4661 | 247 | 8958-9701 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | phage Gp37Gp68 familyprotein | ||||||||||
| Protein Sequence: | MSTQTSIEWT EMTWNPVVGC TKVSPGCKHC YAENMARRLQ AMGTPGYENG FRLSLRPEKL QEPLQRKKPT IYFVNSMSDL FHEQVPDTYI DQVFAVIHQA SQHTFQILTK RAERLADYFS QRTPPANAWL GVSVEDREYG VPRIDCLRGI NAAIRFLSAE PLLEDLGELN LTDIHWVIVG GESGAKARPM KQEWVDNIHR QCDAFGAAFF FKQWGGWGSD GVKRSKKANG RLLKGQTWDE IPLLTLA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpS | TnpS | Tn4661 | 323 | 9731-10702 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | cointegrate resolution | ||||||||||
| Sequence Family: | TyrosineSite-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Tyrosine | ||||||||||
| Comment: | similar to orfI integrase-like protein of the Pseudomonas sp. transposon Tn5041 | ||||||||||
| Protein Sequence: | MKDVERYLQA GTRENTRRSY QSAVEHFEVT WGGFLPATSD SIVRYLVDYA DTLSLNTLKQ RLAALAQWHI RHGFPDPTKT PTVHQVLKGI RTLHPSQTKQ AAPLQLQHLE HAVEWLEQEA MCLFQAEDLA GYLRCRRDTA LLLIGFWRGF RSDELCRLQV EHIQAEAGAG MTLFLPWSKG DRDNLGTTHY VPALRRLCPV QAYLDWIGAA GIARGMVFRR LDRWGNLNEA GLNPGSLIVL LRQILQRAGI QADLYTSHSL RRGFATWATA NGWDIKALMT YVGWKDIKSA MRYIDPAISF GGLAARPALE LNASTLTQVP ASH |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpT | TnpT | Tn4661 | 333 | 10883-11884 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | cointegrate resolution | ||||||||||
| Comment: | similar to orfQ of the Pseudomonas sp. transposon Tn5041 | ||||||||||
| Protein Sequence: | MARGGINKAL VQKARQAILA RGENPSIDAI RVELGNTGSK TTIHRYLKEL EDADRGRNAA ALPLSEQLAN LVAQLADQLK EEAQADMAQE REQLARERLD YQNQIRQAES RIQQLEGQRA GLTEQFQAAQ QALLQEQQLR QQAEVENARL QQANHDQEAR LQDRDGQIRS LEDKHQHARD ALEHYRQASK EQREQEQRRH ESQVQQLQLE LRQLQQTLIV KQDELTHLNR DNARLLAEAR QQQKDQHAQQ KLLTQKAQAL EVAQNTLTSI VRTNEALEQR CHALQDEVTR LGEASSIQAQ QTQSLQERLA KATAQLKLLG QAPPTSSGGA SSS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | WP_031628629.1 | WP_031628629.1 | Tn4661 | 100 | 12177-12479 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Protein Sequence: | MEHTAAQPVE TVVDITCDVC RQSTSVEGYG QQFGTLQAHW GYGSKHDGER YKVHLCELWF FRTLSGLRRE RMVNAMFDEE PDSTKDEFGL VRRDEFWGDG |
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1. Yano H, Genka H, Ohtsubo Y, Nagata Y, Top EM, Tsuda M.
Cointegrate-resolution of toluene-catabolic transposon Tn4651: determination of crossover site and the segment required for full resolution activity.. Plasmid. 2013 Jan;69(1):24-35. doi: 10.1016/j.plasmid.2012.07.004. Epub 2012 Aug 1. PubMed ID:
22878084
.