Mobile element type:
Transposon
Name:
Tn4659
Synonyms:
Accession:
Tn4659-NC_008275
Family:
Tn3
Group:
Tn21
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Pseudomonas putida MT53
Date of Isolation:
2001
Country:
Molecular Source:
plasmid pWW53
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGGCGCCT CAGAAAAGGG ATTTTAAAGT ACGCTAAG |
| IRR | 3710-3747 | - | 38 | GGGGTCGCCT CAGAAAAGGG ATTTCAAAGT ACGCTAAG |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | Tn4659 | 623-744 | + | 122 | GGCAAAATGT CTCATAAGTC GATTTTTAGA TCTTCCGTCT ATGAAGTAGC GATAAACGAA TTAATAGACG TCTTGGGAGG ATCACTTTAC CGTGTCCACA AACTTATGAG AAAGGAGACT GT |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpR | Tn4659 | 77-622 | - | Accessory Gene | Resolvase |
| tnpA | Tn4659 | 745-3714 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn4659 | 181 | 77-622 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MRLFGYARVS TSQQSLDVQI KALKAENVRA TRIFTDKVSG SHVNREGLRM LRLKVEEGDV VLVKKLDRLG RDTADMIQLI KEFDDMGVAI RFLDDGISTE GTMGKMVVTI LSAVAQAERL RILERTNEGR LEAKAKGVKF GRKPTVDKAE VFTLHGQGIS AMEIAKRLKI GRSTVYKVLA S |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn4659 | 989 | 745-3714 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPRRSILSVA ERESLLALPD SKDDLIRHYS FSDADLTLIS QRRGSANRLG FAVQLCYMRY PGVTLAVDEE PFAPLLRLVA TQLKVPPEAW ADYGQRAETR REHLLELQAL FDFKPFTTRH YRPCVHSLDE LAWQSDKGFV LATELVKGLR QKNILLPSLS VIERICAEAI TRANRRIYET LSEPLSNTHL GCLDGLLKRR DKGQTTWLAW LRQSPVKPNS RHMLEHIERL KAWQALDLPA GIERLVHQNR LLKIAREGGQ MTPADLAKFE PQRRYATLVA LVIEGMATVT DEIIDLHDRI LGKLFNAAKN KHQKQFQASG KAINAKVRLY GRIGQVLLDA KQNGGDPFAA IEAVMSWDAF AKSVTEAQKL AQPEDFDFLH HIGDSYTTLR RYAPQFLDVL KLRAAPAAKD VFDGIEVLRA MNINNARKVP VDAPTSFIKK RWKKLVITDV GIDRRYYELC ALSELKNALR SGDIWVQGSR QFKDFEDYLV PPEKFARLKQ TSELPLAVAT DCDKYLHDRL TLLEAQLAIV NRMALANDLP DAIITESGLK ITPLDAAVPE TAQALIDQTA MALPHIKITE LLLEVDEWTG FTRHFAHLKS GDEAKDKYLL LSTLLADAIN LGLTKMAESC PGTTYAKLSW LQAWHIRDET YSTALAELVN AQLRHPFATH WGDGTTSSSD GQNFRTGNKA ESTGHINPKY GSSPGRTFYT HISDQYAPFH IKVVNVGLRD STYVLDGLLY HEADLRIEEH YTDTAGFTDH VFGLMHLLGF RFAPRIRDLG DTKLFIPKGN MTYDGLKSMI SKDKLDIKAI RTHWDEILRL ATSIKQGTVT ASLMLRKLGS YPRQNGLAVA LRELGRIERT LFILDWLQSV ELRRRVNAGL NKGEARNALA RAVFFNRLGE IRDRSFEQQR YRASGLNLVT AAIVLWNTVY LERSANALHG HGHAVDESLL QYLSPLGWEH INLTGDYLWR RNTKVGAGKF RPLRRLQLA |
||||||||||
1. Miyakoshi M, Shintani M, Inoue K, Terabayashi T, Sai F, Ohkuma M, Nojiri H, Nagata Y, Tsuda M.
ParI, an orphan ParA family protein from Pseudomonas putida KT2440-specific genomic island, interferes with the partition system of IncP-7 plasmids.. Environ Microbiol. 2012 Nov;14(11):2946-59. doi: 10.1111/j.1462-2920.2012.02861.x. Epub 2012 Aug 28. PubMed ID:
22925377
.
2. Yano H, Garruto CE, Sota M, Ohtsubo Y, Nagata Y, Zylstra GJ, Williams PA, Tsuda M. Complete sequence determination combined with analysis of transposition/site-specific recombination events to explain genetic organization of IncP-7 TOL plasmid pWW53 and related mobile genetic elements.. J Mol Biol. 2007 May 25;369(1):11-26. doi: 10.1016/j.jmb.2007.02.098. Epub 2007 Mar 14. PubMed ID: 17408691 .
3. Tsuda M, Genka H. Identification and characterization of Tn4656, a novel class II transposon carrying a set of toluene-degrading genes from TOL plasmid pWW53.. J Bacteriol. 2001 Nov;183(21):6215-24. doi: 10.1128/JB.183.21.6215-6224.2001. PubMed ID: 11591664 .
4. Sentchilo VS, Perebituk AN, Zehnder AJ, van der Meer JR. Molecular diversity of plasmids bearing genes that encode toluene and xylene metabolism in Pseudomonas strains isolated from different contaminated sites in Belarus.. Appl Environ Microbiol. 2000 Jul;66(7):2842-52. doi: 10.1128/AEM.66.7.2842-2852.2000. PubMed ID: 10877777 .
5. Gallegos MT, Williams PA, Ramos JL. Transcriptional control of the multiple catabolic pathways encoded on the TOL plasmid pWW53 of Pseudomonas putida MT53.. J Bacteriol. 1997 Aug;179(16):5024-9. doi: 10.1128/jb.179.16.5024-5029.1997. PubMed ID: 9260942 .
6. Assinder SJ, De Marco P, Osborne DJ, Poh CL, Shaw LE, Winson MK, Williams PA. A comparison of the multiple alleles of xylS carried by TOL plasmids pWW53 and pDK1 and its implications for their evolutionary relationship.. J Gen Microbiol. 1993 Mar;139(3):557-68. doi: 10.1099/00221287-139-3-557. PubMed ID: 8473862 .
7. Assinder SJ, de Marco P, Sayers JR, Shaw LE, Winson MK, Williams PA. Identical resolvases are encoded by Pseudomonas TOL plasmids pWW53 and pDK1.. Nucleic Acids Res. 1992 Oct 25;20(20):5476. doi: 10.1093/nar/20.20.5476. PubMed ID: 1331988 .
8. Osborne DJ, Pickup RW, Williams PA. The presence of two complete homologous meta pathway operons on TOL plasmid pWW53.. J Gen Microbiol. 1988 Nov;134(11):2965-75. doi: 10.1099/00221287-134-11-2965. PubMed ID: 3076178 .
9. Keil H, Keil S, Williams PA. Molecular analysis of regulatory and structural xyl genes of the TOL plasmid pWW53-4.. J Gen Microbiol. 1987 May;133(5):1149-58. doi: 10.1099/00221287-133-5-1149. PubMed ID: 3309179 .
10. Keil H, Saint CM, Williams PA. Gene organization of the first catabolic operon of TOL plasmid pWW53: production of indigo by the xylA gene product.. J Bacteriol. 1987 Feb;169(2):764-70. doi: 10.1128/jb.169.2.764-770.1987. PubMed ID: 3027047 .
11. Keil H, Keil S, Pickup RW, Williams PA. Evolutionary conservation of genes coding for meta pathway enzymes within TOL plasmids pWW0 and pWW53.. J Bacteriol. 1985 Nov;164(2):887-95. doi: 10.1128/jb.164.2.887-895.1985. PubMed ID: 2997136 .
2. Yano H, Garruto CE, Sota M, Ohtsubo Y, Nagata Y, Zylstra GJ, Williams PA, Tsuda M. Complete sequence determination combined with analysis of transposition/site-specific recombination events to explain genetic organization of IncP-7 TOL plasmid pWW53 and related mobile genetic elements.. J Mol Biol. 2007 May 25;369(1):11-26. doi: 10.1016/j.jmb.2007.02.098. Epub 2007 Mar 14. PubMed ID: 17408691 .
3. Tsuda M, Genka H. Identification and characterization of Tn4656, a novel class II transposon carrying a set of toluene-degrading genes from TOL plasmid pWW53.. J Bacteriol. 2001 Nov;183(21):6215-24. doi: 10.1128/JB.183.21.6215-6224.2001. PubMed ID: 11591664 .
4. Sentchilo VS, Perebituk AN, Zehnder AJ, van der Meer JR. Molecular diversity of plasmids bearing genes that encode toluene and xylene metabolism in Pseudomonas strains isolated from different contaminated sites in Belarus.. Appl Environ Microbiol. 2000 Jul;66(7):2842-52. doi: 10.1128/AEM.66.7.2842-2852.2000. PubMed ID: 10877777 .
5. Gallegos MT, Williams PA, Ramos JL. Transcriptional control of the multiple catabolic pathways encoded on the TOL plasmid pWW53 of Pseudomonas putida MT53.. J Bacteriol. 1997 Aug;179(16):5024-9. doi: 10.1128/jb.179.16.5024-5029.1997. PubMed ID: 9260942 .
6. Assinder SJ, De Marco P, Osborne DJ, Poh CL, Shaw LE, Winson MK, Williams PA. A comparison of the multiple alleles of xylS carried by TOL plasmids pWW53 and pDK1 and its implications for their evolutionary relationship.. J Gen Microbiol. 1993 Mar;139(3):557-68. doi: 10.1099/00221287-139-3-557. PubMed ID: 8473862 .
7. Assinder SJ, de Marco P, Sayers JR, Shaw LE, Winson MK, Williams PA. Identical resolvases are encoded by Pseudomonas TOL plasmids pWW53 and pDK1.. Nucleic Acids Res. 1992 Oct 25;20(20):5476. doi: 10.1093/nar/20.20.5476. PubMed ID: 1331988 .
8. Osborne DJ, Pickup RW, Williams PA. The presence of two complete homologous meta pathway operons on TOL plasmid pWW53.. J Gen Microbiol. 1988 Nov;134(11):2965-75. doi: 10.1099/00221287-134-11-2965. PubMed ID: 3076178 .
9. Keil H, Keil S, Williams PA. Molecular analysis of regulatory and structural xyl genes of the TOL plasmid pWW53-4.. J Gen Microbiol. 1987 May;133(5):1149-58. doi: 10.1099/00221287-133-5-1149. PubMed ID: 3309179 .
10. Keil H, Saint CM, Williams PA. Gene organization of the first catabolic operon of TOL plasmid pWW53: production of indigo by the xylA gene product.. J Bacteriol. 1987 Feb;169(2):764-70. doi: 10.1128/jb.169.2.764-770.1987. PubMed ID: 3027047 .
11. Keil H, Keil S, Pickup RW, Williams PA. Evolutionary conservation of genes coding for meta pathway enzymes within TOL plasmids pWW0 and pWW53.. J Bacteriol. 1985 Nov;164(2):887-95. doi: 10.1128/jb.164.2.887-895.1985. PubMed ID: 2997136 .