Mobile element type:
Transposon
Name:
Tn4430
Synonyms:
Accession:
Tn4430-X07651.1
Family:
Tn3
Group:
Tn4430
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
B. thuringiensis
Date of Isolation:
1988
Country:
France
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGTACCGC CAGCATTTCG GAAAAAAACC ACGCTAAG |
| IRR | 4112-4149 | - | 38 | GGGGTACCGC CAGCATTTCG GAAAAAAACC ACGCTAAG |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | Tn4430 | 113-228 | + | 116 | TAATACAACA CAATATTAAT TGTGTTGTAT TAGGTGTTAT AATAAATATA AATCTAGGGG TTTAACGCAA CACAATTTAT CGATAAATAA ATACTTTTAG ACGCAACACA ATTTAT |
| res_site_IR1 | Tn4430 | 113-126 | + | 14 | TAATACAACA CAAT |
| res_site_IR2 | Tn4430 | 131-144 | - | 14 | TAATACAACA CAAT |
| res_site_DR1 | Tn4430 | 175-192 | + | 18 | TAACGCAACA CAATTTAT |
| res_site_DR2 | Tn4430 | 210-228 | + | 19 | TAGACGCAAC ACAATTTAT |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpI | Tn4430 | 247-1101 | + | Accessory Gene | Resolvase |
| tnpA | Tn4430 | 1120-4083 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpI | TnpI | Tn4430 | 284 | 247-1101 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | resolvase | ||||||||||
| Sequence Family: | TyrosineSite-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Tyrosine | ||||||||||
| Protein Sequence: | MDVAKQFSSY LKQENKTENT VQGYTSGIRQ YIKWFEGSYD RKLTKLYRQN ILEYISYLKN VKMLNAKSIN HKISSLAKFN EFLIQKGSQQ DQVILKTDMI KVQTVYASPT QIVELDVKKF LQSVLEDNNK RNYAIATLLA YTGVRISEAL SIKMNDFNLQ TGECIIRSGK GGKQRIVLLN SKVLSAIKDY LIDRKTYSTA HESPYLFISK KREKLDRTVV NRIFKSYSNV ITPHQLRHFF CTNAIEKGFS IHEVANQAGH SNIHTTLLYT NPNQLQLKNK MELL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn4430 | 987 | 1120-4083 | + |
|---|
| Class: | Transposase | ||||||||||
| Function: | transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MGVKQLLSEA QRNELMDLSR LTEWDLVTFR TFSKHDLHLI LKHRRGYNRL GFALRLVLIR YPGWSLTEYK DIPQYVVAYV TSRLRIPPEE FLVYAKRGNT LWEHLGEIRT EYGYQNFSSE YKETLLQFLV QQAMDNNNTL YLIEITISTL RKTKVILPAM YVIEDIVWEA KQQADQKVYS ILHDGLVQEQ KDQLDALLLP TINGKSPLAW LKDVPAQPSP ESFLKVIDRL QFVQKIGLTI DTTKINTNRL RQLARLGSKY EPYAFRRFNE VKRYSMLVSF LLEITQDLID YAIEIHDRLM MNLQTKGKKE QDEIQQANGK KLNEKILQFI TVCGTLIEAK ETGKDAFAAL DEVMSWNEMV ESVEEAKQLS RPLNYDYLDL LNTRYSYVRR YAPTLLRSLH FRATKSGEPV LQALDTIHEL NETGKRKVPH GAPLHFVSNR WQKHVYDDDG NINRHYYELA ALTELRNHIR SGDIFVSGSR HHKAFDDYLI PYDEWNEVSN IPNGLTAPLK AEDYITDRIN RLNEHLEWLS KNSEKLEGVD ISQGKLHVER LDRGTPEEAK AFSKLLHSML PRIKLTDLLI EVASWTGFHD QFIHASTNQS PDQEEQNIVL ATLMAMGTNI GLTKMAEATP GISYRQMANA SQWRMYDDAM VRAQSILVNF QKEQKLSSYW GDGTTSSSDG MRLSIAVRSL HADSNPHYGT GKGGTIYRFV SDQLSAYHVK VITTNARDAL HVLDGLLHHE TDLKIEEHYT DTAGYTDQVF ALTHLLGFRF APRIRDLADT KLFSIPGGEE YENVQALLKG KINVKLIKEN YEDIRRLAYS VQTGKVSSAL IMGKLGSYAR QNKLATALGE MGRIEKTLFT LDYISNKAVR RRVQKGLNKG EAINALARTI FFGQRGEFRE RALQDQLQRA SALNIIINAI SVWNTVYMEK AVEELKARGE FREDLMPYAW PLGWEHINFL GEYKFEGLHD TGQMNLRPLR IKEPFYS |
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1. Mahillon J, Lereclus D.
Structural and functional analysis of Tn4430: identification of an integrase-like protein involved in the co-integrate-resolution process.. EMBO J. 1988 May;7(5):1515-26. doi: 10.1002/j.1460-2075.1988.tb02971.x. PubMed ID:
2842151
.
2. Lereclus D, Menou G, Lecadet MM. Isolation of a DNA sequence related to several plasmids from Bacillus thuringiensis after a mating involving the Streptococcus faecalis plasmid pAM beta 1.. Mol Gen Genet. 1983;191(2):307-13. doi: 10.1007/BF00334831. PubMed ID: 6413823 .
2. Lereclus D, Menou G, Lecadet MM. Isolation of a DNA sequence related to several plasmids from Bacillus thuringiensis after a mating involving the Streptococcus faecalis plasmid pAM beta 1.. Mol Gen Genet. 1983;191(2):307-13. doi: 10.1007/BF00334831. PubMed ID: 6413823 .